3x DYKDDDDK Tag

发表于2024年10月21日由Whatman官网

3x DYKDDDDK Tag;

3x DYKDDDDK Tag 是研究抗体 (Abs) 不可用的蛋白质的功能和定位的有用工具。通常，它也以 3X 标志形式 (3x DYKDDDDK Tag TFA) 用于纯化低丰度蛋白。

3x DYKDDDDK Tag Chemical Structure

CAS No. : 921765-76-6

规格		是否有货
100 mg	;	询价	;
250 mg	;	询价	;
500 mg	;	询价	;

* Please select Quantity before adding items.

生物活性	3x DYKDDDDK Tag is a useful tool for investigating the function and localization of proteins whose antibodies (Abs) are not available. Often it is also used in a 3X FLAG format (3x DYKDDDDK Tag) for purifying difficult proteins that accumulate in low abundance ^[1]^[2].
体外研究 (In Vitro)	HEK293 cells transiently transfected with HARS-expressing plasmids were harvested and lysed in CelLytic M buffer containing mammalian protease inhibitor cocktail for 20 min at 4 °C. FLAG-tagged HARS is purified by binding to an anti-DYDDDDK resin per manufacturer protocol and eluted by competition with 3X-DYKDDDDK peptide in buffer containing 50 mM Tris-HCl pH 7.4, and 150 mM NaCl (purification of human HARS)^[2]. MCE has not independently confirmed the accuracy of these methods. They are for reference only.
分子量	3002.88
Formula	D₁₅K₆Y₃
CAS 号	921765-76-6
Sequence Shortening	DYKDDDDKDYKDDDDKDYKDDDDK
运输条件	Room temperature in continental US; may vary elsewhere.
储存方式	Please store the product under the recommended conditions in the Certificate of Analysis.
参考文献	[1]. Mishra V. Affinity Tags for Protein Purification. Curr Protein Pept Sci. 2020;21(8):821-830. [2]. Abbott JA, et al. The Usher Syndrome Type IIIB Histidyl-tRNA Synthetase Mutation Confers Temperature Sensitivity. Biochemistry. 2017;56(28):3619-3631.

EpiSight™ BisulTaq™ DNA Polymerase, recombinant, Solution Ver.2 EpiSight™BisulTaq™DNA聚合酶，重组溶液Ver.2 品牌：FUJIFILM Wako

发表于2024年7月28日由Whatman官网

EpiSight™ BisulTaq™ DNA Polymerase, recombinant, Solution Ver.2

EpiSight™BisulTaq™DNA聚合酶，重组溶液Ver.2

品牌：FUJIFILM Wako
CAS No.：
储存条件：-20℃
纯度：–

产品编号 (生产商编号)	等级	规格	运输包装	零售价(RMB)	库存情况	参考值
293-78701	for Genetic Research	20 tests	–		–	–

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3x DYKDDDDK Tag TFA

发表于2024年7月21日由Whatman官网

3x DYKDDDDK Tag TFA;

DYKDDDDK peptide (FLAG) 是研究抗体 (Abs) 不可用的蛋白质的功能和定位的有用工具。通常，它也以 3X 标志形式 (3x DYKDDDDK Tag TFA) 用于纯化低丰度蛋白。

3x DYKDDDDK Tag TFA Chemical Structure

规格		是否有货
100 mg	;	询价	;
250 mg	;	询价	;
500 mg	;	询价	;

* Please select Quantity before adding items.

生物活性	DYKDDDDK peptide (FLAG) is a useful tool for investigating the function and localization of proteins whose antibodies (Abs) are not available. Often it is also used in a 3X FLAG format (3x DYKDDDDK Tag TFA) for purifying difficult proteins that accumulate in low abundance ^[1]^[2].
体外研究 (In Vitro)	HEK293 cells transiently transfected with HARS-expressing plasmids were harvested and lysed in CelLytic M buffer containing mammalian protease inhibitor cocktail for 20 min at 4 °C. FLAG-tagged HARS is purified by binding to an anti-DYDDDDK resin per manufacturer protocol and eluted by competition with 3X-DYKDDDDK peptide in buffer containing 50 mM Tris-HCl pH 7.4, and 150 mM NaCl (purification of human HARS)^[2]. MCE has not independently confirmed the accuracy of these methods. They are for reference only.
分子量	3116.90
Formula	D₁₅K₆Y₃
Sequence Shortening	DYKDDDDKDYKDDDDKDYKDDDDK
运输条件	Room temperature in continental US; may vary elsewhere.
储存方式	Please store the product under the recommended conditions in the Certificate of Analysis.
参考文献	[1]. Mishra V. Affinity Tags for Protein Purification. Curr Protein Pept Sci. 2020;21(8):821-830. [2]. Abbott JA, et al. The Usher Syndrome Type IIIB Histidyl-tRNA Synthetase Mutation Confers Temperature Sensitivity. Biochemistry. 2017;56(28):3619-3631.

日本同仁化学Hampton蛋白结晶试剂盒CrystalWand™ Magnetic/HR4-729| DOJINDO

发表于2024年6月13日由Whatman官网

上海金畔生物科技有限公司代理日本同仁化学 DOJINDO代理商全线产品，欢迎访问官网了解更多信息

上海金畔生物代理Hampton research品牌蛋白结晶试剂耗材工具等，我们将竭诚为您服务，欢迎访问Hampton research官网或者咨询我们获取更多相关Hampton research品牌产品信息。

Products > Cryocrystallography > Cryo Tools > CrystalWand™ Magnetic

CrystalWand™ Magnetic

CAT NO

HR4-729

NAME

CrystalWand Magnetic, Straight

DESCRIPTION

each

PRICE

$83.00

应用

CrystalCap Magnetic & CrystalCap Copper Magnetic handling tool
CrystalCap Magnetic 和 CrystalCap Copper 磁性处理工具

描述

The CrystalWand Magnetic is designed to be used exclusively with the CrystalCap, CrystalCap ALS, and CrystalCap SPINE systems during transfer of the caps from the vial to the goniometer head and from the goniometer to the vial. The 6 1/2” (165 mm) long chrome plated steel wand features a plastic housing enclosing a spring tensioned plunger that when depressed, moves a non-magnetic steel platform away from the magnet housed in the end of the wand. This causes the steel CrystalCap to detach from the wand and be placed readily into the vial. When the platform is retracted, the wand securely holds the steel CrystalCap Magnetic.

CrystalWand Magnetic 专为 CrystalCap、CrystalCap ALS 和 CrystalCap SPINE 系统而设计，可在将瓶盖从样品瓶转移到测角仪头部以及从测角仪转移到样品瓶期间使用。 6 1/2 英寸（165 毫米）长的镀铬钢棒具有一个塑料外壳，其中包含一个弹簧拉紧的柱塞，当按下该柱塞时，将一个非磁性钢平台从棒末端的磁铁移开。这会导致钢 CrystalCap 从棒上分离并轻松放入小瓶中。当平台缩回时，魔杖会牢固地固定钢 CrystalCap Magnetic。

HR4-729 CrystalWand Magnetic

日本同仁化学Hampton蛋白结晶试剂盒CrystalCap™| DOJINDO

发表于2024年6月13日由Whatman官网

上海金畔生物科技有限公司代理日本同仁化学 DOJINDO代理商全线产品，欢迎访问官网了解更多信息

上海金畔生物代理Hampton research品牌蛋白结晶试剂耗材工具等，我们将竭诚为您服务，欢迎访问Hampton research官网或者咨询我们获取更多相关Hampton research品牌产品信息。Products > Cryocrystallography > CrystalCap Systems > CrystalCap™

CrystalCap™

Applications

Cryocrystallography

Features

SSRL style sample mount for cryocrystallography
CrystalCap attaches magnetically to CrystalCap Vial and Magnetic Goniometer Base
Conical shape with ledge compatible with SSRL style grippers, auto mounters and sample handlers

Description

The CrystalCap is a magnetic sample mount (also known as a cap, pin or goniometer base) designed for cryocrystallography systems that accept SSRL style sample mounts. The CrystalCap attaches to a magnetic CrystalCap Vial and Magnetic Goniometer Base. The tip of the CrystalCap accepts a Mounted CryoLoop™.

The CrystalCap Vial is a 1.8 ml cryo vial featuring two small vents. A ring magnet is molded into the open end of the vial so that when the cap is positioned in the vial, the ring magnet holds the cap on the vial during cryogenic storage.The HR4-904 CrystalCap Vial does not have a magnet on the bottom of the vial.

The conical shape with ledge is compatible with SSRL style grippers, auto mounters and sample handlers.

Compatible with the following Synchrotron Radiation Beamlines

North & South America
• The Advanced Light Source, Berkeley, California ALS 4.2.2, ALS 11.3.1, ALS 12.2.2, ALS 12.3.2
• The Advanced Photon Source, Argonne, Illinois APS 14-BM-C BioCARS, APS 14-ID-B BioCARS
• Cornell High Energy, Synchrotron Source, Ithaca, New York CHESS A1, CHESS F1
• Canadian Light Source, Saskatchewan, Canada, CLS 08ID-1, CLS 08B1-1
• Stanford Synchrotron Radiation Laboratory, Menlo Park, California SSRL BL7-1, SSRL BL9-1, SSRL BL9-2, SSRL BL11-1, SSRL BL12-2, SSRL BL14-1

Asia & Australia
• Shanghai Synchrotron Radiation Facility, Shanghai, China SSRF BL17U1
• National Synchrotron Radiation Research Center, Taiwan NSRRC BL13B1, NSRRC BL13C1
• Pohang Accelerator Laboratory, Pohang, South Korea PAL 2D, PAL 5C, PAL 7A
• Photon Factory, Tsukuba, Japan PF BL-5A, PF BL-17A, PF AR-NW12A
• Super Photon ring-8 GeV, Japan SPRING-8 BL12B2, SPRING-8 BL24XU, SPRING-8 BL26B1, SPRING-8 BL26B2, SPRING-8 BL32B2, SPRING-8 BL32XU, SPRING-8 BL38B1, SPRING-8 BL41XU, SPRING-8 BL44B2, SPRING-8 BL44XU

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cart quote

Support Material(s)

CrystalCap Drawing with Dimensions

Synchrotron Beamline Compatibility

References

日本同仁化学Hampton蛋白结晶试剂盒CrystalCap™ ALS| DOJINDO

发表于2024年6月12日由Whatman官网

上海金畔生物科技有限公司代理日本同仁化学 DOJINDO代理商全线产品，欢迎访问官网了解更多信息

CrystalCap™ ALS

Applications

Cryocrystallography

Features

ALS style crystal mount for cryocrystallography
CrystalCap ALS attaches magnetically to CrystalCap Vial and Magnetic Goniometer Base
Ledge free conical shape compatible with ALS style grippers, auto mounters and sample handlers

Description

The CrystalCap ALS is a magnetic sample mount (also known as a cap, pin or goniometer base) designed for cryocrystallography systems that accept ALS style sample mounts. The CrystalCap ALS attaches to a magnetic CrystalCap Vial and magnetic goniometer base. The tip of the CrystalCap accepts a Mounted CryoLoop™.

CrystalCap ALS (HR4-779) is supplied without a vial (HR4-904).

The CrystalCap Vial is a 1.8 ml cryo vial featuring two small vents. A ring magnet is molded into the open end of the vial so that when the cap is positioned in the vial, the ring magnet holds the cap on the vial during cryogenic storage. The HR4-904 CrystalCap Vial does not have a magnet on the bottom of the vial.

The ledge free conical shape of the CrystalCap ALS is compatible with ALS style grippers, auto mounters and sample handlers.

ALS style sample mounters accept the CrystalCap ALS.

Compatible with the following Synchrotron Radiation Beamlines

North & South America
• The Advanced Light Source, Berkeley, California ALS 4.2.2, ALS 5.0.1, ALS 5.0.2, ALS 5.0.3, ALS 8.2.1, ALS 8.3.1, ALS 11.3.1, ALS 12.2.2, ALS 12.3.2, ALS 12.3.1-APX
• The Advanced Photon Source, Argonne, Illinois APS 14-BM-C BioCARS, APS 14-ID-B BioCARS, APS 17-ID IMCA-CAT, APS 19-BM, APS 19-ID, APS 22-BM SER-CAT, APS 22-ID SER-CAT, PS 23-BM-B GA/CA, APS 23-ID-B GA/CA, APS 23-ID-D GA/CA, APS 24-ID-C NE-CAT, APS 24-ID-E NE-CAT, APS 31-ID LR-CAT
• Center for Advanced Microstructures and Devices, Baton Rouge, Louisiana CAMD GCPCC
• Cornell High Energy, Synchrotron Source, Ithaca, New York CHESS A1, CHESS F1
• Canadian Light Source, Saskatchewan, Canada, CLS 08ID-1, CLS 08B1-1
• The Brazilian Synchrotron, Light Laboratory, Sao Paulo, Brazil LNLS D03B-MX1, LNLS W01B-MX2
• Stanford Synchrotron Radiation Laboratory, Menlo Park, California SSRL BL7-1, SSRL BL9-1, SSRL BL9-2, SSRL BL11-1, SSRL BL12-2, SSRL BL14-1

Europe
• Kurchatov Center for Synchrotron Radiation and Nanotechnology, Moscow, Russia KCSRNT K4.4
• Max-Lab, Lund University, Sweden MAX II I711, MAX II I911-2, MAX II I911-3
• MPG/DESY, Hamburg, Germany MPGDESY BW6
• SOLEIL, Saint-Aubin, France SOLEIL PROXIMA1, SOLEIL PROXIMA2

Asia & Australia
• Shanghai Synchrotron Radiation Facility, Shanghai, China SSRF BL17U1
• National Synchrotron Radiation Research Center, Taiwan NSRRC BL13B1, NSRRC BL13C1
• Pohang Accelerator Laboratory, Pohang, South Korea PAL 2D, PAL 5C, PAL 7A
• Photon Factory, Tsukuba, Japan PF BL-5A, PF BL-17A, PF AR-NW12A • Super Photon ring-8 GeV, Japan SPRING-8 BL12B2, SPRING-8 BL24XU, SPRING-8 BL26B1, SPRING-8 BL26B2, SPRING-8 BL32B2, SPRING-8 BL32XU, SPRING-8 BL38B1, SPRING-8 BL41XU, SPRING-8 BL44B2, SPRING-8 BL44XU

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