0.05mol/L Tris-HCl Buffer Solution (pH7.6) 0.05mol/L Tris-HCl缓冲溶液(pH7.6) 品牌:FUJIFILM Wako


0.05mol/L Tris-HCl Buffer Solution (pH7.6)

0.05mol/L Tris-HCl缓冲溶液(pH7.6)

品牌:FUJIFILM Wako
CAS No.:
储存条件:暗所
纯度:
产品编号

(生产商编号)

等级 规格 运输包装 零售价(RMB) 库存情况 参考值

208-14691

for Pathology Research 1L 580.00


* 干冰运输、大包装及大批量的产品需酌情添加运输费用


* 零售价、促销产品折扣、运输费用、库存情况、产品及包装规格可能因各种原因有所变动,恕不另行通知,确切详情请联系上海金畔生物科技有限公司。

日本同仁化学Hampton蛋白结晶试剂盒Tris Buffer/HR2-589/HR2-725| DOJINDO

上海金畔生物科技有限公司代理日本同仁化学 DOJINDO代理商全线产品,欢迎访问官网了解更多信息

上海金畔生物代理Hampton research品牌蛋白结晶试剂耗材工具等,我们将竭诚为您服务,欢迎访问Hampton research官网或者咨询我们获取更多相关Hampton research品牌产品信息。

Products > Optimize Reagents > Optimize – Buffers > Tris Buffer

Tris Buffer

Hampton蛋白结晶试剂盒Tris Buffer/HR2-589/HR2-725

CAT NO

HR2-589

NAME

1.0 M Tris

DESCRIPTION

100 mL

PRICE

$60.00

CAT NO

HR2-725

NAME

1.0 M Tris pH 8.5

DESCRIPTION

100 mL

PRICE

$60.00

应用

  • Crystallization grade Tris for formulating screens or for optimization
  • 结晶级 Tris 用于配制筛选或优化

特征

  • Sterile filtered
  • Formulated in Type 1+ ultrapure water: 18.2 megaohm-cm resistivity at 25°C, < 5 ppb Total Organic Carbon, bacteria free (<1 Bacteria (CFU/ml)), pyrogen free (<0.03 Endotoxin (EU/ml)), RNase-free (< 0.01 ng/mL) and DNase-free (< 4 pg/µL)
  • 无菌过滤
    在 1+ 型超纯水中配制:25°C 时电阻率为 18.2 兆欧厘米,总有机碳 < 5 ppb,无细菌(<1 细菌 (CFU/ml)),无热原(<0.03 内毒素 (EU/ml)) , 无 RNase (< 0.01 ng/mL) 和无 DNase (< 4 pg/µL)

描述Tris

Synonyms: Trizma base or 2-Amino-2-(hydroxymethyl)-1,3-propanediol or THAM or Tris base or Tris(hydroxymethyl)aminomethane or Trometamol
C4H11NO3
NH2C(CH2OH)3
Mr 121.14
CAS Number [77-86-1]
EC Number 201-064-4
Beilstein Registry Number 741883
Merck 14,9772
RTECS TY2900000
MDL Number MFCD00004679
PubChem Substance ID 24889854
Purity 99.8 – 100.1%
pKa = 8.1 at 25°C

Useful pH range of Tris: 7.0 – 9.0

Titrate HR2-589 to the desired pH using HCl
HR2-589 Measured pH Range: 10.9 – 11.1 at 25°C
HR2-589 Measured Conductivity Range: 193.3 – 236.7 µS/cm at 25°C
HR2-589 Measured Refractive Index: 1.34991 – 1.35058 at 20°C

HR2-725 is titrated to pH 8.5 using HCl
HR2-725 Measured Conductivity Range: 18.1 – 22.7 mS/cm at 25°C
HR2-725 Measured Refractive Index: 1.35310 – 1.35369 at 20°C

同义词: Trizma 碱或 2-Amino-2-(羟甲基)-1,3-丙二醇或 THAM 或 Tris 碱或 Tris(羟甲基)氨基甲烷或氨丁三醇
C4H11NO3
NH2C(CH2OH)3
121.14 先生
CAS 编号 [77-86-1]
欧盟编号 201-064-4
贝尔斯坦注册号 741883
默克 14,9772
RTECS TY2900000
MDL 编号 MFCD00004679
PubChem 物质 ID 24889854
纯度 99.8 – 100.1%
pKa = 8.1 在 25°C

Tris 的有用 pH 值范围:7.0 – 9.0

使用 HCl 将 HR2-589 滴定至所需的 pH 值
HR2-589 测得的 pH 范围:25°C 时为 10.9 – 11.1
HR2-589 测量的电导率范围:25°C 时为 193.3 – 236.7 µS/cm
HR2-589 实测折射率:20°C 时为 1.34991 – 1.35058

使用 HCl 将 HR2-725 滴定至 pH 8.5
HR2-725 测量的电导率范围:25°C 时为 18.1 – 22.7 mS/cm
HR2-725 实测折射率:20°C 时为 1.35310 – 1.35369

Insoluble Matter 0.005% max
DNase None Detected
RNase None Detected
Protease None Detected
Heavy Metals 5 ppm max
As  ≤ 5 ppm max
Ca  ≤ 5 ppm max
Cu  ≤ 5 ppm max
Fe  ≤ 5 ppm max
Pb  ≤ 5 ppm max
Mg ≤ 5 ppm max

Trizma is a registered trademark of Sigma-Aldrich Co.

支持材料

Hampton蛋白结晶试剂盒Tris Buffer/HR2-589/HR2-725 HR2-589 Tris SDSHampton蛋白结晶试剂盒Tris Buffer/HR2-589/HR2-725 HR2-725 Tris pH 8.5 SDSHampton蛋白结晶试剂盒Tris Buffer/HR2-589/HR2-725 Titration Table

相关项目

  • Hydrochloric acid
  • StockOptions Tris Buffer Kit
  • Individual StockOptions Tris Reagents
  • 盐酸
    StockOptions Tris 缓冲套件
    个人股票期权 Tris 试剂

参考

1. Purification and crystallization of Pseudomonas aeruginosa chloramphenicol acetyltransferase. Tian, Y; Beaman, TW; Roderick, SL; Proteins 28, 298- 300, 1997.

1. 铜绿假单胞菌氯霉素乙酰转移酶的纯化与结晶。 田,Y; 比曼,台湾; 罗德里克,SL; 蛋白质 28, 298-300, 1997。

日本同仁化学Hampton蛋白结晶试剂盒TRIS hydrochloride Buffer/HR2-579/HR2-727| DOJINDO

上海金畔生物科技有限公司代理日本同仁化学 DOJINDO代理商全线产品,欢迎访问官网了解更多信息

上海金畔生物代理Hampton research品牌蛋白结晶试剂耗材工具等,我们将竭诚为您服务,欢迎访问Hampton research官网或者咨询我们获取更多相关Hampton research品牌产品信息。

Products > Optimize Reagents > Optimize – Buffers > TRIS hydrochloride Buffer

TRIS hydrochloride Buffer

Hampton蛋白结晶试剂盒TRIS hydrochloride Buffer/HR2-579/HR2-727

CAT NO

HR2-579

NAME

1.0 M TRIS hydrochloride

DESCRIPTION

100 mL

PRICE

$63.00

CAT NO

HR2-727

NAME

1.0 M TRIS hydrochloride pH 8.5

DESCRIPTION

100 mL

PRICE

$63.00

支持材料

Hampton蛋白结晶试剂盒TRIS hydrochloride Buffer/HR2-579/HR2-727 HR2-579 TRIS hydrochloride SDSHampton蛋白结晶试剂盒TRIS hydrochloride Buffer/HR2-579/HR2-727 HR2-727 TRIS hydrochloride pH 8.5 SDSHampton蛋白结晶试剂盒TRIS hydrochloride Buffer/HR2-579/HR2-727 Titration Table

应用

  • Crystallization grade TRIS hydrochloride buffer for formulating screens or for optimization
  • 用于配制筛选或优化的结晶级 TRIS 盐酸盐缓冲液

特征

  • Sterile filtered
  • Formulated in Type 1+ ultrapure water: 18.2 megaohm-cm resistivity at 25°C, < 5 ppb Total Organic Carbon, bacteria free (<1 Bacteria (CFU/ml)), pyrogen free (<0.03 Endotoxin (EU/ml)), RNase-free (< 0.01 ng/mL) and DNase-free (< 4 pg/µL)
  • 无菌过滤
    在 1+ 型超纯水中配制:25°C 时电阻率为 18.2 兆欧厘米,总有机碳 < 5 ppb,无细菌(<1 细菌 (CFU/ml)),无热原(<0.03 内毒素 (EU/ml)) , 无 RNase (< 0.01 ng/mL) 和无 DNase (< 4 pg/µL)

描述TRIS hydrochloride

Synonyms: TRIS HCl or Trizma hydrochloride or Tris(hydroxymethyl)aminomethane hydrochloride
NH2C(CH2OH)3 • HCl
C4H11NO3 • HCl
Mr 157.60
[1185-53-1]
EC Number 214-684-5
Beilstein Registry Number 3675235
MDL Number MFCD00012590
PubChem Substance ID 24900398
Purity ≥ 99.0%

Titrate HR2-579 to the desired pH using NaOH
HR2-579 Measured pH Range: 3.9 – 4.0 at 25°C
HR2-579 Measured Conductivity Range: 52.3 – 53.7 mS/cm at 25°C
HR2-579 Measured Refractive Index Range: 1.35840 – 1.35891 at 20°C

HR2-727 has been titrated to pH 8.5 using NaOH
HR2-727 Measured Conductivity Range: 59.8 – 61.7 mS at 25°C
HR2-727 Measured Refractive Index Range: 1.35938 – 1.36019 at 20°C

Use StockOptions Tris Hydrochloride kit (HR2-237) for a set of 21 Tris hydrochloride reagents titrated to pH 7.0 to 9.0 in 0.1 pH increments.

同义词:TRIS HCl 或 Trizma 盐酸盐或 Tris(羟甲基)氨基甲烷盐酸盐
NH2C(CH2OH)3 • HCl
C4H11NO3 • HCl
157.60 先生
[1185-53-1]
欧盟编号 214-684-5
贝尔斯坦注册号 3675235
MDL 编号 MFCD00012590
PubChem 物质 ID 24900398
纯度≥99.0%

使用 NaOH 将 HR2-579 滴定至所需的 pH 值
HR2-579 测量的 pH 范围:25°C 时 3.9 – 4.0
HR2-579 测量的电导率范围:25°C 时为 52.3 – 53.7 mS/cm
HR2-579 测量的折射率范围:20°C 时为 1.35840 – 1.35891

HR2-727 已使用 NaOH 滴定至 pH 8.5
HR2-727 测得的电导率范围:25°C 时为 59.8 – 61.7 mS
HR2-727 测量的折射率范围:20°C 时为 1.35938 – 1.36019

将 StockOptions Tris 盐酸试剂盒 (HR2-237) 用于一套 21 种 Tris 盐酸试剂,以 0.1 的 pH 增量滴定至 pH 7.0 至 9.0。

Al  ≤0.0005%
As ≤0.0001%
Ba ≤0.0005%
Bi  ≤0.0005%
Ca ≤0.001%
Cd ≤0.0005%
Co ≤0.0005%
Cr  ≤0.0005%
Cu ≤0.0005%
Fe  ≤0.0005%
K   ≤0.005%
Li   ≤0.0005%
Mg ≤0.0005%
Mn ≤0.0005%
Mo ≤0.0005%
Na ≤0.005%
Ni  ≤0.0005%
Pb  ≤0.0005%
SO4 ≤0.005%
Sr  ≤0.0005%
Zn  ≤0.0005%

TRIS hydrchoride

相关项目

  • Sodium hydroxide
  • StockOptions Tris Hydrochloride Buffer Kit
  • Individual StockOptions Tris Hydrochloride Reagents
  • 氢氧化钠
    StockOptions Tris 盐酸缓冲液试剂盒
    个别股票期权 Tris 盐酸盐试剂

日本同仁化学Hampton蛋白结晶试剂盒BIS-TRIS Buffer| DOJINDO

上海金畔生物科技有限公司代理日本同仁化学 DOJINDO代理商全线产品,欢迎访问官网了解更多信息

上海金畔生物代理Hampton research品牌蛋白结晶试剂耗材工具等,我们将竭诚为您服务,欢迎访问Hampton research官网或者咨询我们获取更多相关Hampton research品牌产品信息。

Products > Optimize Reagents > Optimize – Buffers > BIS-TRIS Buffer

BIS-TRIS Buffer

应用

  • Crystallization grade BIS-TRIS buffer for formulating screens or for optimization
  • 用于配制筛选或优化的结晶级 BIS-TRIS 缓冲液

特征

  • Sterile filtered
  • Formulated in Type 1+ ultrapure water: 18.2 megaohm-cm resistivity at 25°C, < 5 ppb Total Organic Carbon, bacteria free (<1 Bacteria (CFU/ml)), pyrogen free (<0.03 Endotoxin (EU/ml)), RNase-free (< 0.01 ng/mL) and DNase-free (< 4 pg/µL)
  • 无菌过滤
    在 1+ 型超纯水中配制:25°C 时电阻率为 18.2 兆欧厘米,总有机碳 < 5 ppb,无细菌(<1 细菌 (CFU/ml)),无热原(<0.03 内毒素 (EU/ml)) , 无 RNase (< 0.01 ng/mL) 和无 DNase (< 4 pg/µL)

描述 BIS-TRIS

Synonyms: 2,2-Bis(hydroxymethyl)-2,2′,2″”-nitrilotriethanol, or 2-Bis(2-hydroxyethyl)amino-2-(hydroxymethyl)-1,3-propanediol, or Bis(2-hydroxyethyl)amino-tris(hydroxymethyl)methane.
C8H19NO5
Mr 209.24
CAS Number [6976-37-0]
EC Number 230-237-7
Beilstein Registry Number 2205275
MDL Number MFCD00002853
Purity ≥ 98.0%
pKa = 6.4 at 20°C
pKa = 6.5 at 25°C

HR2-781 is titrated to pH 5.5 using HCl
Measured Conductivity Range of HR2-781: 38.4 – 43.1 mS/cm at 25°C
Measured Refractive Index of HR2-781: 1.37069 – 1.37150 at 20°C

HR2-783 is titrated to pH 6.5 using HCl
Measured Conductivity Range of HR2-783: 22.8 – 28.7 mS/cm at 25°C
Measured Refractive Index of HR2-783: 1.36817 – 1.36926 at 20°C

同义词:2,2-双(羟甲基)-2,2′,2″”-次氮基三乙醇,或 2-双(2-羟乙基)氨基-2-(羟甲基)-1,3-丙二醇,或双(2- 羟乙基)氨基-三(羟甲基)甲烷。
C8H19NO5
209.24 先生
CAS 编号 [6976-37-0]
欧盟编号 230-237-7
贝尔斯坦注册号 2205275
MDL 编号 MFCD00002853
纯度≥98.0%
pKa = 6.4 在 20°C
pKa = 6.5 在 25°C

使用 HCl 将 HR2-781 滴定至 pH 5.5
HR2-781 的测量电导率范围:25°C 时为 38.4 – 43.1 mS/cm
HR2-781 的实测折射率:20°C 时为 1.37069 – 1.37150

HR2-783 使用 HCl 滴定至 pH 6.5
HR2-783 的测量电导率范围:22.8 – 28.7 mS/cm 在 25°C
HR2-783 的实测折射率:20°C 时为 1.36817 – 1.36926

Al  ≤0.0005%
Ca ≤0.0005%
Cl  ≤0.05%
Cu ≤0.0005%
Fe  ≤0.0005%
K   ≤0.005%
Mg ≤0.0005%
NH4 ≤0.05%
Na  ≤0.005%
Pb  ≤0.001%
SO4 ≤0.05%
Zn  ≤0.0005%

BIS-TRIS

Hampton蛋白结晶试剂盒BIS-TRIS Buffer

CAT NO

HR2-781

NAME

1.0 M BIS-TRIS pH 5.5

DESCRIPTION

100 mL

PRICE

$169.00

CAT NO

HR2-783

NAME

1.0 M BIS-TRIS pH 6.5

DESCRIPTION

100 mL

PRICE

$169.00

相关项目

  • Hydrochloric acid
  • StockOptions Bis-Tris Buffer Kit
  • Individual StockOptions Bis-Tris Reagents
  • 盐酸
    StockOptions Bis-Tris 缓冲套件
    个别股票期权 Bis-Tris 试剂

参考

1. Atomic structure of the degraded procapsid particle of the bacteriophage G4: induced structural changes in the presence of calcium ions and functional implications. McKenna, R; Bowman, BR; Ilag, LL; Rossmann, MG; Fane, BA. J Mol Biol 256, 736- 750, 1996.

2. Expression, crystallization and preliminary X-ray diffraction study of FtsY, the docking protein of the signal recognition particle of E. coli. Montoya, G; Svensson, C; Luirink, J; Sinning, I. Proteins 28, 285- 288, 1997.

1. 噬菌体 G4 降解前衣壳颗粒的原子结构:在钙离子存在下诱导结构变化和功能影响。 麦肯纳,R; 鲍曼,BR; 伊拉格,LL; 罗斯曼,MG; 法恩,文学士。 分子生物学杂志 256, 736-750, 1996。

2.大肠杆菌信号识别颗粒对接蛋白FtsY的表达、结晶和初步X射线衍射研究。 蒙托亚,G; 斯文森,C; 卢林克,J; Sinning, I. 蛋白质 28, 285-288, 1997。

日本同仁化学Hampton蛋白结晶试剂盒BIS-TRIS propane Buffer| DOJINDO

上海金畔生物科技有限公司代理日本同仁化学 DOJINDO代理商全线产品,欢迎访问官网了解更多信息

上海金畔生物代理Hampton research品牌蛋白结晶试剂耗材工具等,我们将竭诚为您服务,欢迎访问Hampton research官网或者咨询我们获取更多相关Hampton research品牌产品信息。

Products > Optimize Reagents > Optimize – Buffers > BIS-TRIS propane Buffer

BIS-TRIS propane Buffer

应用

  • Crystallization grade BIS-TRIS propane pH 7.0 for formulating screens or for optimization
  • 结晶级 BIS-TRIS 丙烷 pH 7.0,用于配制筛选或优化

特征

  • Sterile filtered
  • Formulated in Type 1+ ultrapure water: 18.2 megaohm-cm resistivity at 25°C, < 5 ppb Total Organic Carbon, bacteria free (<1 Bacteria (CFU/ml)), pyrogen free (<0.03 Endotoxin (EU/ml)), RNase-free (< 0.01 ng/mL) and DNase-free (< 4 pg/µL)
  • 无菌过滤
    在 1+ 型超纯水中配制:25°C 时电阻率为 18.2 兆欧厘米,总有机碳 < 5 ppb,无细菌(<1 细菌 (CFU/ml)),无热原(<0.03 内毒素 (EU/ml)) , 无 RNase (< 0.01 ng/mL) 和无 DNase (< 4 pg/µL)

描述 BIS-TRIS propane

Synonyms: 1,3-Bis[tris(hydroxymethyl)methylamino]propane
C11H26N2O6
CH2[CH2NHC(CH2OH)3]2
Mr 282.34
CAS Number [64431-96-5]
EC Number 264-899-3
Beilstein Registry Number 1786109
MDL Number MFCD00004689
PubChem Substance ID 24891934
Purity ≥ 99.0%
pKa1 = 6.8 at 25°C
pKa2 = 9.0 at 25°C

HR2-795 is titrated to pH 7.0 using HCl
Measured Conductivity Range: 41.9 – 43.5 mS/cm at 25°C
Measured Refractive Index Range: 1.39110 – 1.39222 at 20°C

别名:1,3-双[三(羟甲基)甲氨基]丙烷
C11H26N2O6
CH2[CH2NHC(CH2OH)3]2
282.34 先生
CAS 编号 [64431-96-5]
欧盟编号 264-899-3
贝尔斯坦注册号 1786109
MDL 编号 MFCD00004689
PubChem 物质 ID 24891934
纯度≥99.0%
pKa1 = 6.8 在 25°C
pKa2 = 9.0 在 25°C

HR2-795 使用 HCl 滴定至 pH 7.0
测得的电导率范围:25°C 时 41.9 – 43.5 mS/cm
测得的折射率范围:20°C 时为 1.39110 – 1.39222

BIS-TRIS propane

Hampton蛋白结晶试剂盒BIS-TRIS propane Buffer

CAT NO

HR2-795

NAME

1.0 M BIS-TRIS propane pH 7.0

DESCRIPTION

100 mL

PRICE

$108.00

相关项目

  • Individual StockOptions Bis-Tris Propane Reagents
  • Citric acid BIS-TRIS propane Buffer
  • 个别股票期权 Bis-Tris 丙烷试剂
    柠檬酸 BIS-TRIS 丙烷缓冲液

参考

1. Two-fold symmetry of crystalline DNA-EcoRI endonuclease recognition complexes. Grable, J; Frederick, CA; Samudzi, C; Jen-Jacobson, L; Lesser, D; Greene, P; Boyer, HW; Itakura, K; Rosenberg, JM. J Biomol Struct Dyn 1, 1149- 1160, 1984.

2. Non-native Local Interactions in Protein Folding and Stability: Introducing a Helical Tendency in the AII beta-Sheet alpha-Spectrin SH3 Domain. Prieto, J; Wilmans, M; Jimenez, MA; Rico, M; Serrano, L. J Mol Biol 268, 760- 778, 1997.

1. 结晶 DNA-EcoRI 核酸内切酶识别复合物的双重对称性。 格拉布尔,J; 加利福尼亚州弗雷德里克; 萨穆兹,C; 仁-雅各布森,L; 较小,D; 格林,P; 博耶,HW; 板仓,K; 罗森伯格,JM。 J Biomol Struct Dyn 1, 1149-1160, 1984。

2. 蛋白质折叠和稳定性中的非天然局部相互作用:在 AII β-片 α-Spectrin SH3 域中引入螺旋趋势。 普列托,J; 威尔曼斯,M; 马萨诸塞州希门尼斯; 里科,M; Serrano, L. J Mol Biol 268, 760-778, 1997。

荧光染料Tris(4-aminophenyl)methane(Synonyms: Leucopararosaniline)

荧光染料Dye Reagents Tris(4-aminophenyl)methane;(Synonyms: Leucopararosaniline)

Tris(4-aminophenyl)methane 是一种三苯甲烷类染料。Tris(4-aminophenyl)methane 是一种稍弱的 HCV 解旋酶 (HCV helicase) 抑制剂。

Tris(4-aminophenyl)methaneamp;;(Synonyms: Leucopararosaniline)

Tris(4-aminophenyl)methane Chemical Structure

CAS No. : 548-61-8

规格 价格 是否有货 数量
10;mM;*;1 mL in DMSO ¥495 In-stock
100 mg ¥450 In-stock
250 mg ¥880 In-stock
500 mg ¥1200 In-stock
1 g ; 询价 ;
5 g ; 询价 ;

* Please select Quantity before adding items.

Tris(4-aminophenyl)methane 相关产品

bull;相关化合物库:

  • Bioactive Compound Library Plus
  • Anti-Infection Compound Library
  • Antiviral Compound Library
  • Anti-Hepatitis C Virus Compound Library

生物活性

Tris(4-aminophenyl)methane is a triphenylmethane dye. Tris(4-aminophenyl)methane is a weak HCV helicase inhibitor.

IC50 Target

HCV helicase[1]

体外研究
(In Vitro)

Tris(4-aminophenyl)methane (Compound 8) shows weak HCV helicase inhibition (30% inhibition at 100 μM)[1]. To preserve RNA in a biological sample for analysis, the sample is incubated with an RNA preservative capable of precipitating RNA in an aqueous solution, such as a triphenylmethane dye (e.g., methyl green, crystal violet, pararosaniline, or Tris(4-aminophenyl)methane), cresyl violet, or cobalt ions. RNA preservation may be used in an immunostaining assay and other histochemical methods[2].

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

分子量

289.37

Formula

C19H19N3

CAS 号

548-61-8

运输条件

Room temperature in continental US; may vary elsewhere.

储存方式

4deg;C, protect from light

*In solvent : -80deg;C, 6 months; -20deg;C, 1 month (protect from light)

溶解性数据
In Vitro:;

DMSO : 50 mg/mL (172.79 mM; Need ultrasonic)

配制储备液
浓度 溶剂体积 质量 1 mg 5 mg 10 mg
1 mM 3.4558 mL 17.2789 mL 34.5578 mL
5 mM 0.6912 mL 3.4558 mL 6.9116 mL
10 mM 0.3456 mL 1.7279 mL 3.4558 mL

*

请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液;一旦配成溶液,请分装保存,避免反复冻融造成的产品失效
储备液的保存方式和期限:-80°C, 6 months; -20°C, 1 month (protect from light)。-80°C 储存时,请在 6 个月内使用,-20°C 储存时,请在 1 个月内使用。

In Vivo:

请根据您的实验动物和给药方式选择适当的溶解方案。以下溶解方案都请先按照 In Vitro 方式配制澄清的储备液,再依次添加助溶剂:

——为保证实验结果的可靠性,澄清的储备液可以根据储存条件,适当保存;体内实验的工作液,建议您现用现配,当天使用; 以下溶剂前显示的百
分比是指该溶剂在您配制终溶液中的体积占比;如在配制过程中出现沉淀、析出现象,可以通过加热和/或超声的方式助溶

  • 1.

    请依序添加每种溶剂:;10% DMSO ;; 40% PEG300 ;; 5% Tween-80 ;; 45% saline

    Solubility: ≥ 2.5 mg/mL (8.64 mM); Clear solution

    此方案可获得 ≥ 2.5 mg/mL (8.64 mM,饱和度未知) 的澄清溶液。

    以 1 mL 工作液为例,取 100 μL 25.0 mg/mL 的澄清 DMSO 储备液加到 400 μL PEG300 中,混合均匀;向上述体系中加入50 μL Tween-80,混合均匀;然后继续加入 450 μL生理盐水定容至 1 mL。

    将 0.9 g 氯化钠,完全溶解于 100 mL ddH₂O 中,得到澄清透明的生理盐水溶液

  • 2.

    请依序添加每种溶剂:;10% DMSO ;; 90% (20% SBE-β-CD in saline)

    Solubility: ≥ 2.5 mg/mL (8.64 mM); Clear solution

    此方案可获得 ≥ 2.5 mg/mL (8.64 mM,饱和度未知) 的澄清溶液。

    以 1 mL 工作液为例,取 100 μL 25.0 mg/mL 的澄清 DMSO 储备液加到 900 μL 20% 的 SBE-β-CD 生理盐水水溶液中,混合均匀。

    将 2 g 磺丁基醚 β-环糊精加入 5 mL 生理盐水中,再用生理盐水定容至 10 mL,完全溶解,澄清透明
*以上所有助溶剂都可在 MCE 网站选购。
参考文献
  • [1]. Chen CS, et al. Structure-based discovery of triphenylmethane derivatives as inhibitors of hepatitis C virushelicase. J Med Chem. 2009 May 14;52(9):2716-23.

荧光染料Tris(4-aminophenyl)methane(Synonyms: Leucopararosaniline)

荧光染料Dye Reagents Tris(4-aminophenyl)methane;(Synonyms: Leucopararosaniline)

Tris(4-aminophenyl)methane 是一种三苯甲烷类染料。Tris(4-aminophenyl)methane 是一种稍弱的 HCV 解旋酶 (HCV helicase) 抑制剂。

Tris(4-aminophenyl)methane(Synonyms: Leucopararosaniline)

Tris(4-aminophenyl)methane Chemical Structure

CAS No. : 548-61-8

规格 价格 是否有货 数量
10;mM;*;1 mL in DMSO ¥495 In-stock
100 mg ¥450 In-stock
250 mg ¥880 In-stock
500 mg ¥1200 In-stock
1 g ; 询价 ;
5 g ; 询价 ;

* Please select Quantity before adding items.

Tris(4-aminophenyl)methane 相关产品

bull;相关化合物库:

  • Bioactive Compound Library Plus
  • Anti-Infection Compound Library
  • Antiviral Compound Library
  • Anti-Hepatitis C Virus Compound Library

生物活性

Tris(4-aminophenyl)methane is a triphenylmethane dye. Tris(4-aminophenyl)methane is a weak HCV helicase inhibitor.

IC50 Target

HCV helicase[1]

体外研究
(In Vitro)

Tris(4-aminophenyl)methane (Compound 8) shows weak HCV helicase inhibition (30% inhibition at 100 μM)[1]. To preserve RNA in a biological sample for analysis, the sample is incubated with an RNA preservative capable of precipitating RNA in an aqueous solution, such as a triphenylmethane dye (e.g., methyl green, crystal violet, pararosaniline, or Tris(4-aminophenyl)methane), cresyl violet, or cobalt ions. RNA preservation may be used in an immunostaining assay and other histochemical methods[2].

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

分子量

289.37

Formula

C19H19N3

CAS 号

548-61-8

运输条件

Room temperature in continental US; may vary elsewhere.

储存方式

4deg;C, protect from light

*In solvent : -80deg;C, 6 months; -20deg;C, 1 month (protect from light)

溶解性数据
In Vitro:;

DMSO : 50 mg/mL (172.79 mM; Need ultrasonic)

配制储备液
浓度 溶剂体积 质量 1 mg 5 mg 10 mg
1 mM 3.4558 mL 17.2789 mL 34.5578 mL
5 mM 0.6912 mL 3.4558 mL 6.9116 mL
10 mM 0.3456 mL 1.7279 mL 3.4558 mL

*

请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液;一旦配成溶液,请分装保存,避免反复冻融造成的产品失效
储备液的保存方式和期限:-80°C, 6 months; -20°C, 1 month (protect from light)。-80°C 储存时,请在 6 个月内使用,-20°C 储存时,请在 1 个月内使用。

In Vivo:

请根据您的实验动物和给药方式选择适当的溶解方案。以下溶解方案都请先按照 In Vitro 方式配制澄清的储备液,再依次添加助溶剂:

——为保证实验结果的可靠性,澄清的储备液可以根据储存条件,适当保存;体内实验的工作液,建议您现用现配,当天使用; 以下溶剂前显示的百
分比是指该溶剂在您配制终溶液中的体积占比;如在配制过程中出现沉淀、析出现象,可以通过加热和/或超声的方式助溶

  • 1.

    请依序添加每种溶剂:;10% DMSO ;; 40% PEG300 ;; 5% Tween-80 ;; 45% saline

    Solubility: ≥ 2.5 mg/mL (8.64 mM); Clear solution

    此方案可获得 ≥ 2.5 mg/mL (8.64 mM,饱和度未知) 的澄清溶液。

    以 1 mL 工作液为例,取 100 μL 25.0 mg/mL 的澄清 DMSO 储备液加到 400 μL PEG300 中,混合均匀;向上述体系中加入50 μL Tween-80,混合均匀;然后继续加入 450 μL生理盐水定容至 1 mL。

    将 0.9 g 氯化钠,完全溶解于 100 mL ddH₂O 中,得到澄清透明的生理盐水溶液

  • 2.

    请依序添加每种溶剂:;10% DMSO ;; 90% (20% SBE-β-CD in saline)

    Solubility: ≥ 2.5 mg/mL (8.64 mM); Clear solution

    此方案可获得 ≥ 2.5 mg/mL (8.64 mM,饱和度未知) 的澄清溶液。

    以 1 mL 工作液为例,取 100 μL 25.0 mg/mL 的澄清 DMSO 储备液加到 900 μL 20% 的 SBE-β-CD 生理盐水水溶液中,混合均匀。

    将 2 g 磺丁基醚 β-环糊精加入 5 mL 生理盐水中,再用生理盐水定容至 10 mL,完全溶解,澄清透明
*以上所有助溶剂都可在 MCE 网站选购。
参考文献
  • [1]. Chen CS, et al. Structure-based discovery of triphenylmethane derivatives as inhibitors of hepatitis C virushelicase. J Med Chem. 2009 May 14;52(9):2716-23.

Bis-Tris

生化分析试剂 Biochemical Assay Reagents
Bis-Tris;

Bis-Tris 是一种常用的缓冲液。

Bis-Tris

Bis-Tris Chemical Structure

CAS No. : 6976-37-0

规格 价格 是否有货 数量
Free Sample (0.1-0.5 mg) ; Apply now ;
25 g ¥500 In-stock
50 g ; 询价 ;

* Please select Quantity before adding items.

Bis-Tris 相关产品

bull;相关化合物库:

  • Bioactive Compound Library Plus

生物活性

Bis-Tris is a commonly used buffer[1].

分子量

209.24

Formula

C8H19NO5

CAS 号

6976-37-0

运输条件

Room temperature in continental US; may vary elsewhere.

储存方式
Powder -20deg;C 3 years
4deg;C 2 years
In solvent -80deg;C 6 months
-20deg;C 1 month
溶解性数据
In Vitro:;

H2O : 500 mg/mL (2389.60 mM; Need ultrasonic)

配制储备液
浓度 溶剂体积 质量 1 mg 5 mg 10 mg
1 mM 4.7792 mL 23.8960 mL 47.7920 mL
5 mM 0.9558 mL 4.7792 mL 9.5584 mL
10 mM 0.4779 mL 2.3896 mL 4.7792 mL

*

请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液;一旦配成溶液,请分装保存,避免反复冻融造成的产品失效
储备液的保存方式和期限:-80°C, 6 months; -20°C, 1 month。-80°C 储存时,请在 6 个月内使用,-20°C 储存时,请在 1 个月内使用。

参考文献
  • [1]. Oh, Soon Jin, et al. Structure and phosphodiesterase activity of Bis-Tris coordinated lanthanide(III) complexes.

SeraCare (KPL) 5460-0016 10X TRIS-GLYCINE-SDS BUFFER, 5 L         旧货号51-11-02 10X Tris-甘氨酸-SDS缓冲液 1 Bottle x 5 L

上海金畔生物科技有限公司提供SeraCare (KPL) 5460-0016 10X TRIS-GLYCINE-SDS BUFFER, 5 L         旧货号51-11-02 10X Tris-甘氨酸-SDS缓冲液 1 Bottle x 5 L,欢迎访问官网了解更多产品信息和订购

品牌 货号 产品名称 规格 价格

SeraCare (KPL) 5460-0016 10X TRIS-GLYCINE-SDS BUFFER, 5 L         旧货号51-11-02 10X Tris-甘氨酸-SDS缓冲液 1 Bottle x 5 L 3386.00

SeraCare (KPL) 5460-0015 10X TRIS-GLYCINE-SDS BUFFER, 1 L         旧货号51-11-01 10X Tris-甘氨酸-SDS缓冲液 1 Bottle x 1.0 L 1513.00

SeraCare (KPL) 5460-0012 10X TRIS-GLYCINE TRANSFER BUFFER, 5 L    旧货号51-10-02 10X Tris-甘氨酸转膜液 1 Bottle x 5 L 3386.00

SeraCare (KPL) 5460-0011 10X TRIS-GLYCINE TRANSFER BUFFER, 1 L    旧货号51-10-01 10X Tris-甘氨酸转膜液 1 Bottle x 1.0 L 1082.00

SeraCare (KPL) 5450-0012 SILVER ENHANCER KIT FOR MEMBRANE         旧货号50-22-02 WB 银离子增强剂 1 x 250 mL Silver Enhancer MB Solution A, 1x 250 mL, Silver Enhancer MB Solution B 5153.00

SeraCare (KPL) 5450-0011 HRP GOAT ANTI-MOUSE IGG (H+L), 1.0 ML    旧货号474-1806 HRP标记抗小鼠二抗 1 Vial x 1.0 mL 2027.00

SeraCare (KPL) 5450-0010 HRP GOAT ANTI-RABBIT IGG (H+L), 1.0 ML   旧货号474-1506 HRP标记抗兔二抗 1 Vial x 1.0 mL 2027.00

SeraCare (KPL) 5450-0009 HRP GOAT ANTI-HUMAN IGG (H+L), 1.0 ML    旧货号474-1006 HRP标记抗人二抗 1 Vial x 1.0 mL 2027.00

SeraCare (KPL) 5430-0055 PHOSPHAGLO AP SUBSTRATE, 100 ML          旧货号55-60-04 WB AP 底物 1 Bottle x 100 mL 5446.00

SeraCare (KPL) 5430-0054 PHOSPHAGLO AP SUBSTRATE, 30 ML           旧货号55-60-03 WB AP 底物 1 Bottle x 30 mL 2043.00

SeraCare (KPL) 5430-0053 PHOSPHAGLO RESERVE AP SUBSTRATE, 100 ML  旧货号55-60-02 WB AP 高灵敏底物 1 Bottle x 100 mL 8090.00

SeraCare (KPL) 5430-0052 PHOSPHAGLO RESERVE AP SUBSTRATE, 30 ML   旧货号55-60-01 WB AP 高灵敏底物 1 Bottle x 30 mL 2800.00

SeraCare (KPL) 5430-0051 LUMIGLO RESERVE™ CHEMI. SUBSTRATE, 50 ML 旧货号54-71-02 LumiGLO 高灵敏度化学发光底物 1 x 100mL 20X Wash Solution, 1 x 17 mL LumiGLO Reserve Chemiluminescent Substarte A, 1 x 34 mL LumiGLO Reserve Chemiluminescent Substrate B 6493.00

SeraCare (KPL) 5430-0050 LUMIGLO RESERVE CHEMI. SUBSTRATE,600 CM2 旧货号54-71-01 LumiGLO 高灵敏度化学发光底物 2 x 100 mL Wash Buffer, 1 x 10mL Solution A, & 1 x 20mL Solution B 4071.00

SeraCare (KPL) 5430-0049 LUMIGLO RESERVE CHEMI. SUBSTRATE,2400CM2 旧货号54-71-00 LumiGLO 高灵敏度化学发光底物 2 x 200 mL Wash Buffer, 1 x 40mL Solution A, & 1 x 80mL Solution B 10856.00

SeraCare (KPL) 5430-0042 LUMIGLO CHEMI. SUBSTRATE, 60 ML          旧货号54-61-02 LumiGLO 化学发光底物 2*30 mL 1220.00

SeraCare (KPL) 5430-0041 LUMIGLO CHEMI. SUBSTRATE KIT, 720 ML     旧货号54-61-01 LumiGLO 化学发光底物 720ml 6682.00

SeraCare (KPL) 5430-0040 LUMIGLO CHEMI. SUBSTRATE, 240 ML         旧货号54-61-00 LumiGLO 化学发光底物 1 X 120 mL LumiGLO Chemiluminescent Substrate A & 1 x 120 mL LumiGLO Chemiluminescent Substrate B 3109.00

SeraCare (KPL) 5420-0040 FIREPHOS™ MEMBRANE AP SUBS.100ml *DISC   100ml 1193.00

SeraCare (KPL) 5420-0038 BCIP/NBT AP SUBSTRATE, 100 ML kit        旧货号50-81-18 WB AP BCIP/NBT 1组分显色底物 1 Bottle x 100 mL 1014.00

Hampton HR2-725 1.0 M Tris pH 8.5 100 ml

上海金畔生物科技有限公司提供Hampton HR2-725 1.0 M Tris pH 8.5 100 ml,欢迎访问官网了解更多产品信息和订购

品牌 货号 产品名称 规格 价格

Hampton HR2-725 1.0 M Tris pH 8.5 100 ml 710.00

Hampton HR2-589 1.0 M Tris 100 ml 710.00

Hampton HR2-821 2.0 M Magnesium sulfate heptahydrate 100 ml 830.00

Hampton HR4-126 Replacement #11 Blade 5/pk 139.00

Hampton HR4-326 Four Color Mounting Clay (4 x 4 ounce blocks) 16 oz 139.00

Hampton HR2-519 24% w/v Polyethylene glycol 3,500 200 ml 840.00

Hampton HR2-731 1.0 M Sodium acetate trihydrate pH 4.6 100 ml 480.00

Hampton HR2-789 1.0 M Sodium acetate trihydrate pH 4.5 100 ml 480.00

Hampton HR2-569 1.0 M Sodium acetate trihydrate 100 ml 480.00

Hampton HR2-429-06 Proti-Ace™ Reagent #06 (pack of 3 tubes) 1/ea 967.00

Hampton HR2-429-05 Proti-Ace™ Reagent #05 (pack of 3 tubes) 1/ea 967.00

Hampton HR2-429-04 Proti-Ace™ Reagent #04 (pack of 3 tubes) 1/ea 967.00

Hampton HR2-429-03 Proti-Ace™ Reagent #03 (pack of 3 tubes) 1/ea 967.00

Hampton HR2-429-02 Proti-Ace™ Reagent #02 (pack of 3 tubes) 1/ea 967.00

Hampton HR2-429-01 Proti-Ace™ Reagent #01 (pack of 3 tubes) 1/ea 967.00

Hampton HR2-429-07 Proti-Ace™ Dilution Buffer (pack of 3 tubes) 1/ea 967.00

Hampton HR2-432-06 Proti-Ace™ 2 Reagent #06 (pack of 3 tubes) 1/ea 967.00

Hampton HR2-432-05 Proti-Ace™ 2 Reagent #05 (pack of 3 tubes) 1/ea 967.00

Hampton HR2-432-04 Proti-Ace™ 2 Reagent #04 (pack of 3 tubes) 1/ea 967.00

Hampton HR2-432-03 Proti-Ace™ 2 Reagent #03 (pack of 3 tubes) 1/ea 967.00