TMA-DPH is a hydrophobic fluorescent membrane probe (Ex=355 nm; Em=430 nm).

In contact with cells, TMA-DPH is instantaneously incorporated into the plasma membrane. TMA-DPH is concentrated in lysosomes and highly acidic late endosomes^[1]. The TMA-DPH fluorescence lifetime remains constant, 6.2±0.2 ns, below the same critical concentration of 2 μM, but decreases significantly with higher concentrations. This decrease, however, slows down above 5 μM. The TMA-DPH fluorescence anisotropy displays a similar evolution to the fluorescence lifetime. It is first constant, 0.283±0.003, again until 2 μM, and then decreases rapidly to 0.270±0.003 with 5 μM, continuing to fall, but at a lower rate with higher concentrations^[2].

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

461.62

C₂₈H₃₁NO₃S

115534-33-3

Room temperature in continental US; may vary elsewhere.

-20deg;C, sealed storage, away from moisture and light

*该产品在溶液状态不稳定，建议您现用现配，即刻使用。

• [1]. Illinger D, et al. The kinetic aspects of intracellular fluorescence labeling with TMA-DPH support the maturation model for endocytosis in L929 cells. J Cell Biol. 1994 May;125(4):783-94.

  [2]. Illinger D, et al. A comparison of the fluorescence properties of TMA-DPH as a probe for plasma membrane and for endocytic membrane. Biochim Biophys Acta. 1995 Oct 4;1239(1):58-66.

L929 cells in 2 mL DM10F are allowed to adhere on microscope slide flasks. For the observation of plasma membrane labeling, cells are incubated for a short time (10 s) at room temperature with TMA-DPH 2×10^-6 M in PBS or in DM10F from a 4×10^-3 M stock solution in dimethylformamide. The unwashed slide is then transferred to the microscope and observed. For the labeling of internalized membrane, the cells are incubated in slide flasks at 37°C, with TMA-DPH 2×10^-6 M in DM10F for the desired time, and then washed by gently shaking the slide in PBS for a few seconds^[1].

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

• [1]. Illinger D, et al. The kinetic aspects of intracellular fluorescence labeling with TMA-DPH support the maturation model for endocytosis in L929 cells. J Cell Biol. 1994 May;125(4):783-94.

  [2]. Illinger D, et al. A comparison of the fluorescence properties of TMA-DPH as a probe for plasma membrane and for endocytic membrane. Biochim Biophys Acta. 1995 Oct 4;1239(1):58-66.