6-TAMRA-SE (6-TAMRA-NHS ester) 是一种带有胺反应基的荧光染料。6-TAMRA-SE 是用于 DNA 自动测序的传统荧光团之一。
6-TAMRA-SE Chemical Structure
CAS No. : 150810-69-8
规格
价格
是否有货
数量
10;mM;*;1 mL in DMSO
￥1625
In-stock
1 mg
￥600
In-stock
5 mg
￥1400
In-stock
10 mg
￥2000
In-stock
50 mg
￥7500
In-stock
100 mg
;
询价
;
200 mg
;
询价
;
* Please select Quantity before adding items.
6-TAMRA-SE 相关产品
bull;相关化合物库:
Bioactive Compound Library Plus
生物活性
6-TAMRA-SE (6-TAMRA-NHS ester) is a fluorescent dye carrying the amine reactive group. 6-TAMRA-SE is one of the traditional fluorophores used for automated DNA sequencing[1][2][3].
体外研究 (In Vitro)
6-TAMRA-SE (6-TAMRA-NHS ester) is an amine-reactive form of tetramethylrhodamine. 6-TAMRA-SE (soluble in DMF and DMSO, absorption=546, emission=576 measured in MeOH ϵ=9.5×104ϵ=9.5×104)[1][3].
MCE has not independently confirmed the accuracy of these methods. They are for reference only.
分子量
527.52
Formula
C29H25N3O7
CAS 号
150810-69-8
中文名称
6-羧基四甲基罗丹明琥珀酰亚胺酯
运输条件
Room temperature in continental US; may vary elsewhere.
储存方式
-20deg;C, sealed storage, away from moisture and light
*In solvent : -80deg;C, 6 months; -20deg;C, 1 month (sealed storage, away from moisture and light)
溶解性数据
In Vitro:;
DMSO : 100 mg/mL (189.57 mM; Need ultrasonic)
配制储备液
浓度溶剂体积质量
1 mg
5 mg
10 mg
1 mM
1.8957 mL
9.4783 mL
18.9566 mL
5 mM
0.3791 mL
1.8957 mL
3.7913 mL
10 mM
0.1896 mL
0.9478 mL
1.8957 mL
*
请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液；一旦配成溶液，请分装保存，避免反复冻融造成的产品失效。 储备液的保存方式和期限：-80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light)。-80°C 储存时，请在 6 个月内使用，-20°C 储存时，请在 1 个月内使用。
In Vivo:
请根据您的实验动物和给药方式选择适当的溶解方案。以下溶解方案都请先按照 In Vitro 方式配制澄清的储备液，再依次添加助溶剂：
[1]. Wang Y, et al. RNA molecules that specifically and stoichiometrically bind aminoglycoside antibiotics with high affinities. Biochemistry. 1996 Sep 24;35(38):12338-46.
[2]. Tessmar J, et al. Toward the development of biomimetic polymers by protein immobilization: PEGylation of insulin as a model reaction. Tissue Eng. 2004;10(3-4):441-453.
[3]. Thomas TP, et al. Investigation of tumor cell targeting of a dendrimer nanoparticle using a double-clad optical fiber probe. J Biomed Opt. 2008;13(1):014024.
6-TAMRA-SE (6-TAMRA-NHS ester) 是一种带有胺反应基的荧光染料。6-TAMRA-SE 是用于 DNA 自动测序的传统荧光团之一。
6-TAMRA-SE Chemical Structure
CAS No. : 150810-69-8
规格
价格
是否有货
数量
10;mM;*;1 mL in DMSO
￥1625
In-stock
1 mg
￥600
In-stock
5 mg
￥1400
In-stock
10 mg
￥2000
In-stock
50 mg
￥7500
In-stock
100 mg
;
询价
;
200 mg
;
询价
;
* Please select Quantity before adding items.
6-TAMRA-SE 相关产品
bull;相关化合物库:
Bioactive Compound Library Plus
生物活性
6-TAMRA-SE (6-TAMRA-NHS ester) is a fluorescent dye carrying the amine reactive group. 6-TAMRA-SE is one of the traditional fluorophores used for automated DNA sequencing[1][2][3].
体外研究 (In Vitro)
6-TAMRA-SE (6-TAMRA-NHS ester) is an amine-reactive form of tetramethylrhodamine. 6-TAMRA-SE (soluble in DMF and DMSO, absorption=546, emission=576 measured in MeOH ϵ=9.5×104ϵ=9.5×104)[1][3].
MCE has not independently confirmed the accuracy of these methods. They are for reference only.
分子量
527.52
Formula
C29H25N3O7
CAS 号
150810-69-8
中文名称
6-羧基四甲基罗丹明琥珀酰亚胺酯
运输条件
Room temperature in continental US; may vary elsewhere.
储存方式
-20deg;C, sealed storage, away from moisture and light
*In solvent : -80deg;C, 6 months; -20deg;C, 1 month (sealed storage, away from moisture and light)
溶解性数据
In Vitro:;
DMSO : 100 mg/mL (189.57 mM; Need ultrasonic)
配制储备液
浓度溶剂体积质量
1 mg
5 mg
10 mg
1 mM
1.8957 mL
9.4783 mL
18.9566 mL
5 mM
0.3791 mL
1.8957 mL
3.7913 mL
10 mM
0.1896 mL
0.9478 mL
1.8957 mL
*
请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液；一旦配成溶液，请分装保存，避免反复冻融造成的产品失效。 储备液的保存方式和期限：-80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light)。-80°C 储存时，请在 6 个月内使用，-20°C 储存时，请在 1 个月内使用。
In Vivo:
请根据您的实验动物和给药方式选择适当的溶解方案。以下溶解方案都请先按照 In Vitro 方式配制澄清的储备液，再依次添加助溶剂：
[1]. Wang Y, et al. RNA molecules that specifically and stoichiometrically bind aminoglycoside antibiotics with high affinities. Biochemistry. 1996 Sep 24;35(38):12338-46.
[2]. Tessmar J, et al. Toward the development of biomimetic polymers by protein immobilization: PEGylation of insulin as a model reaction. Tissue Eng. 2004;10(3-4):441-453.
[3]. Thomas TP, et al. Investigation of tumor cell targeting of a dendrimer nanoparticle using a double-clad optical fiber probe. J Biomed Opt. 2008;13(1):014024.
6-TAMRA has been a widely used fluorophore for preparing bioconjugates, especially fluorescent antibody and avidin derivatives used in immunochemistry. Under the name TAMRA, the carboxylic acid of 6-TAMRA has also achieved prominence as a dye for oligonucleotide labeling and automated DNA sequencing applications.
分子量
430.45
Formula
C25H22N2O5
CAS 号
91809-67-5
运输条件
Room temperature in continental US; may vary elsewhere.
5(6)-TAMRA contains a carboxylic acid that can be used to react with primary amines via carbodiimide activation of the carboxylic acid; bright, orange-fluorescent dye produces conjugates with absorption/emission maxima of ~555/580 nm.
分子量
430.45
Formula
C50H44N4O10
CAS 号
98181-63-6
运输条件
Room temperature in continental US; may vary elsewhere.
[1]. Fuchs SM, et al. Pathway for polyarginine entry into mammalian cells. Biochemistry. 2004 Mar 9;43(9):2438-44.
[2]. Gerber D, et al. Insertion and organization within membranes of the delta-endotoxin pore-forming domain, helix 4-loop-helix 5, and inhibition of its activity by a mutant helix 4 peptide. J Biol Chem. 2000 Aug 4;275(31):23602-7.
[3]. Vinayak R, et al. Automated, solid-phase coupling of rhodamine dye acids to 5′ amino oligonucleotides. Nucleic Acids Symp Ser. 2000;(44):257-8.
5-TAMRA-SE is an amine-reactive fluorescent agent, its conjugates yield bright, pH-insensitive orange-red fluorescence (approximate excitation/emission maxima ~546/579) with good photostability.
分子量
527.52
Formula
C29H25N3O7
CAS 号
150810-68-7
中文名称
5-羧基四甲基罗丹明琥珀酰亚胺酯
运输条件
Room temperature in continental US; may vary elsewhere.
储存方式
4deg;C, protect from light, stored under nitrogen
*In solvent : -80deg;C, 6 months; -20deg;C, 1 month (protect from light, stored under nitrogen)
溶解性数据
In Vitro:;
DMF : 10 mg/mL (18.96 mM; Need ultrasonic)
DMSO : 5 mg/mL (9.48 mM; Need ultrasonic and warming)
配制储备液
浓度溶剂体积质量
1 mg
5 mg
10 mg
1 mM
1.8957 mL
9.4783 mL
18.9566 mL
5 mM
0.3791 mL
1.8957 mL
3.7913 mL
10 mM
0.1896 mL
0.9478 mL
1.8957 mL
*
请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液；一旦配成溶液，请分装保存，避免反复冻融造成的产品失效。 储备液的保存方式和期限：-80°C, 6 months; -20°C, 1 month (protect from light, stored under nitrogen)。-80°C 储存时，请在 6 个月内使用，-20°C 储存时，请在 1 个月内使用。
In Vivo:
请根据您的实验动物和给药方式选择适当的溶解方案。以下溶解方案都请先按照 In Vitro 方式配制澄清的储备液，再依次添加助溶剂：
请依序添加每种溶剂：;10% DMF ;; 90% (20% SBE-β-CD in saline)
Solubility: ≥ 1 mg/mL (1.90 mM); Clear solution
*以上所有助溶剂都可在 MCE 网站选购。
参考文献
[1]. Kierzek R, et al.5′-Amino Pyrene Provides a Sensitive, Nonperturbing Fluorescent Probe of RNA Secondary and Tertiary Structure Formation. J Am Chem Soc (1993) 115:4985-4985
[2]. Schmidt T, et al. Local Stoichiometries Determined by Counting Individual Molecules. Anal Chem (1996) 68:4397-4397
5(6)-TAMRA SE is the amine-reactive, mixed isomer form of TAMRA, which is a dye for oligonucleotide labeling and automated DNA sequencing applications.
分子量
527.53
Formula
C58H50N6O14
CAS 号
246256-50-8
中文名称
5(6)-羧基四甲基罗丹明琥珀酰亚胺酯
运输条件
Room temperature in continental US; may vary elsewhere.
储存方式
-20deg;C, protect from light, stored under nitrogen
*In solvent : -80deg;C, 6 months; -20deg;C, 1 month (protect from light, stored under nitrogen)
溶解性数据
In Vitro:;
DMSO : 10 mg/mL (18.96 mM; ultrasonic and warming and heat to 60°C)
配制储备液
浓度溶剂体积质量
1 mg
5 mg
10 mg
1 mM
1.8956 mL
9.4781 mL
18.9563 mL
5 mM
0.3791 mL
1.8956 mL
3.7913 mL
10 mM
0.1896 mL
0.9478 mL
1.8956 mL
*
请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液；一旦配成溶液，请分装保存，避免反复冻融造成的产品失效。 储备液的保存方式和期限：-80°C, 6 months; -20°C, 1 month (protect from light, stored under nitrogen)。-80°C 储存时，请在 6 个月内使用，-20°C 储存时，请在 1 个月内使用。
参考文献
[1]. Fuller ME, et al. Application of a vital fluorescent staining method for simultaneous, near-real-time concentration monitoring of two bacterial strains in an Atlantic coastal plain aquifer in Oyster, Virginia. Appl Environ Microbiol. 2004 Mar;70(3):1680-7.
[2]. Brunner A, et al. Labelling peptides with fluorescent probes for incorporation into degradable polymers. Eur J Pharm Biopharm. 1998 May;45(3):265-73.
[3]. Jiang M, et al. Design and synthesis of new acid cleavable linkers for DNA sequencing by synthesis. Nucleosides Nucleotides Nucleic Acids. 2014;33(12):774-85.
5-TAMRA (5-Carboxytetramethylrhodamine), SE and its conjugates yield bright, pH-insensitive orange-red fluorescence (approximate excitation/emission maxima ~546/579) with good photostability.
分子量
430.45
Formula
C25H22N2O5
CAS 号
91809-66-4
运输条件
Room temperature in continental US; may vary elsewhere.
