标签归档：se

荧光染料BDY FL-X, SE

发表于由

荧光染料Dye Reagents BDY FL-X, SE;

BODIPY FL-X,SE 是一种用于胺标记 (amine) 的荧光绿色染料，荧光量子产率高，对 pH 变化相对不敏感。BODIPY FL-X,SE 可作为 FAM, Cy2 或 FITC 的替代。λabs: 504 nm; λem:510 nm.

BDY FL-X, SE

BDY FL-X, SE Chemical Structure

CAS No. : 217190-09-5

规格 是否有货
100 mg ; 询价 ;
250 mg ; 询价 ;
500 mg ; 询价 ;

* Please select Quantity before adding items.

生物活性

BODIPY FL-X,SE is a fluorescent green dye for the labeling of amine, shows a high fluorescence quantum yield and is relatively insensitive to pH change. BODIPY FL-X,SE can be used as an alternative for FAM, Cy2 or FITC. λabs: 504 nm; λem:510 nm[1].

体外研究
(In Vitro)

BODIPY FL-X, succinimidyl ester dye stock solutions are prepared in DMSO. Then, it is diluted in 0.1 M sodium bicarbonate buffer, pH 8.3, to a final concentration of 10 μM dye.

MCE has not independently confirmed the accuracy of these methods. They are for reference only.
分子量

502.32

Formula

C24H29BF2N4O5
CAS 号

217190-09-5
运输条件

Room temperature in continental US; may vary elsewhere.
储存方式

Please store the product under the recommended conditions in the Certificate of Analysis.
参考文献

  • [1]. K P Top, et al.Green/red dual fluorescence detection of total protein and alkaline phosphate-conjugated probes on blotting membranes. Electrophoresis. 2001 Mar;22(5):896-905.

    [2]. Karen Martin, et al. Simultaneous red/green dual fluorescence detection on electroblots using BODIPY TR-X succinimidyl ester and ELF 39 phosphate.Proteomics. 2002 May;2(5):499-512.

荧光染料TFAX 546,SE triethylammonium

发表于由

荧光染料Dye Reagents TFAX 546,SE triethylammonium;

TFAX 546,SE triethylammonium 是一种胺反应黄色荧光染料，能与蛋白质和抗体形成明亮和光稳定的偶联物。

TFAX 546,SE triethylammonium

TFAX 546,SE triethylammonium Chemical Structure

规格 是否有货
100 mg ; 询价 ;
250 mg ; 询价 ;
500 mg ; 询价 ;

* Please select Quantity before adding items.

生物活性

TFAX 546,SE triethylammonium, an amine reactive yellow fluorescent dye, can forms bright and photostable conjugates with proteins and antibodies[1].

分子量

1159.61

Formula

C50H62Cl3N5O14S3
运输条件

Room temperature in continental US; may vary elsewhere.
储存方式

Please store the product under the recommended conditions in the Certificate of Analysis.
参考文献

  • [1]. Arik J Hone, et al. Alexa Fluor 546-ArIB[V11L;V16A]is a potent ligand for selectively labeling α7 nicotinic acetylcholine receptors. J Neurochem. 2010 Aug; 114(4): 994–1006.

荧光染料Cy5.5-SE(Synonyms: Cyanine5.5 NHS ester)

发表于由

荧光染料Dye Reagents Cy5.5-SE;(Synonyms: Cyanine5.5 NHS ester) 纯度: 96.69%

Cy5.5-SE (Cyanine5.5 NHS ester) 是一种花青染料，可标记多肽、蛋白和寡核苷酸中的氨基。

Cy5.5-SEamp;;(Synonyms: Cyanine5.5 NHS ester)

Cy5.5-SE Chemical Structure

CAS No. : 442912-55-2

规格 价格 是否有货 数量
5 mg ￥6500 In-stock
10 mg ￥10500 询价
50 mg ; 询价 ;
100 mg ; 询价 ;

* Please select Quantity before adding items.

生物活性

Cy5.5-SE (Cyanine5.5 NHS ester) is a cyanine dye, labeling amino-groups in peptides, proteins, and oligonucleotides.

分子量

1014.13

Formula

C45H47N3O16S4
CAS 号

442912-55-2
运输条件

Room temperature in continental US; may vary elsewhere.
储存方式

-20deg;C, sealed storage, away from moisture and light

*In solvent : -80deg;C, 6 months; -20deg;C, 1 month (sealed storage, away from moisture and light)

荧光染料TFAX 488,SE dilithium

发表于由

荧光染料Dye Reagents TFAX 488,SE dilithium;

TFAX 488,SE dilithium 是一种绿色荧光染料，在很宽的范围内表现出pH不敏感性 (pH: 4-10)。TFAX 488,SE dilithium 与蛋白质或抗体 (如山羊抗鼠 IgG、链霉亲和素) 产生明亮、光稳定的结合物。

TFAX 488,SE dilithium

TFAX 488,SE dilithium Chemical Structure

CAS No. : 222164-96-7

规格 是否有货
500 μg 询价

* Please select Quantity before adding items.

生物活性

TFAX 488,SE dilithium is a green fluorescent dye and exhibits pH-insensitivity over a very broad range (pH in the 4-10). TFAX 488,SE dilithium yields exceptionally bright, photostable conjugates with proteins or antibodies (such as goat anti-mouse IgG, streptavidin)[1].

体外研究
(In Vitro)

Excitation maximum=485±11 nm; emission maximum=530±15 nm[1].

MCE has not independently confirmed the accuracy of these methods. They are for reference only.
分子量

643.41

Formula

C26H23Li2N3O13S2
CAS 号

222164-96-7
运输条件

Room temperature in continental US; may vary elsewhere.
储存方式

Please store the product under the recommended conditions in the Certificate of Analysis.
参考文献

  • [1]. Panchuk-Voloshina N, et al. Alexa dyes, a series of new fluorescent dyes that yield exceptionally bright, photostable conjugates. J Histochem Cytochem. 1999;47(9):1179-1188.

荧光染料Janelia Fluor® 526, SE(Synonyms: JF526, SE; JF526, NHS)

发表于由

荧光染料Dye Reagents Janelia Fluor® 526, SE;(Synonyms: JF526, SE; JF526, NHS)

Janelia Fluor® 526, SE (JF526,SE) 是一种含有 NHS 酯基的荧光黄色荧光染料。JF526 是一种用于荧光配体的多功能支架，包括用于遗传编码的自标记蛋白质标签和用于内源性结构的染色。Janelia Fluor® 产品在美国专利下获得许可，来自 Howard Hughes Medical Institute 的 No.9,933,417，10,018,624，10,161,932 和其他专利。

Janelia Fluor® 526, SE(Synonyms: JF526, SE; JF526, NHS)

Janelia Fluor® 526, SE Chemical Structure

规格 是否有货
100 mg ; 询价 ;
250 mg ; 询价 ;
500 mg ; 询价 ;

* Please select Quantity before adding items.

生物活性

Janelia Fluor® 526, SE (JF526,SE) is a fluorogenic yellow fluorescent dye that contains NHS ester group. JF526 is a versatile scaffold for fluorogenic ligands, including labels for genetically encoded self-labeling protein tags and stains for endogenous structures[1]. Janelia Fluor® products are licensed under U.S. Pat. Nos. 9,933,417, 10,018,624 and 10,161,932 and other patents from Howard Hughes Medical Institute.

体外研究
(In Vitro)

Excitation maximum=526 nm; emission maximum=550 nm[1].

MCE has not independently confirmed the accuracy of these methods. They are for reference only.
分子量

659.49

Formula

C31H19F6N3O7
运输条件

Room temperature in continental US; may vary elsewhere.
储存方式

Please store the product under the recommended conditions in the Certificate of Analysis.
参考文献

  • [1]. Zheng Q, et al. Rational Design of Fluorogenic and Spontaneously Blinking Labels for Super-Resolution Imaging [published correction appears in ACS Cent Sci. 2020 Oct 28;6(10):1844]. ACS Cent Sci. 2019;5(9):1602-1613.

荧光染料Cy5.5-SE(Synonyms: Cyanine5.5 NHS ester)

发表于由

荧光染料Dye Reagents Cy5.5-SE;(Synonyms: Cyanine5.5 NHS ester) 纯度: 96.69%

Cy5.5-SE (Cyanine5.5 NHS ester) 是一种花青染料，可标记多肽、蛋白和寡核苷酸中的氨基。

Cy5.5-SE(Synonyms: Cyanine5.5 NHS ester)

Cy5.5-SE Chemical Structure

CAS No. : 442912-55-2

规格 价格 是否有货 数量
5 mg ￥6500 In-stock
10 mg ￥10500 询价
50 mg ; 询价 ;
100 mg ; 询价 ;

* Please select Quantity before adding items.

生物活性

Cy5.5-SE (Cyanine5.5 NHS ester) is a cyanine dye, labeling amino-groups in peptides, proteins, and oligonucleotides.

分子量

1014.13

Formula

C45H47N3O16S4
CAS 号

442912-55-2
运输条件

Room temperature in continental US; may vary elsewhere.
储存方式

-20deg;C, sealed storage, away from moisture and light

*In solvent : -80deg;C, 6 months; -20deg;C, 1 month (sealed storage, away from moisture and light)

荧光染料OG 488, SE(Synonyms: Oregon green 488 succinimidyl ester)

发表于由

荧光染料Dye Reagents OG 488, SE;(Synonyms: Oregon green 488 succinimidyl ester) 纯度: 95.08%

OG 488, SE (Oregon green 488 succinimidyl ester) 是一种荧光 pH 指示剂，是一种基于荧光素的染料，在生物化学和神经科学领域具有许多应用。

OG 488, SEamp;;(Synonyms: Oregon green 488 succinimidyl ester)

OG 488, SE Chemical Structure

CAS No. : 198139-51-4

规格 价格 是否有货 数量
5 mg ￥2300 In-stock
10 mg ￥3600 In-stock
25 mg ￥7200 询价
50 mg ; 询价 ;
100 mg ; 询价 ;

* Please select Quantity before adding items.

OG 488, SE 相关产品

bull;相关化合物库:

  • Bioactive Compound Library Plus

生物活性

OG 488, SE (Oregon green 488 succinimidyl ester), a fluorescent pH indicator, has many applications in biochemistry and neurosciences[1].

分子量

509.37

Formula

C25H13F2NO9
CAS 号

198139-51-4
运输条件

Room temperature in continental US; may vary elsewhere.
储存方式
Powder -20deg;C 3 years
4deg;C 2 years
In solvent -80deg;C 6 months
-20deg;C 1 month
溶解性数据
In Vitro:;

DMSO : 125 mg/mL (245.40 mM; Need ultrasonic)

配制储备液
浓度 溶剂体积 质量 1 mg 5 mg 10 mg
1 mM 1.9632 mL 9.8160 mL 19.6321 mL
5 mM 0.3926 mL 1.9632 mL 3.9264 mL
10 mM 0.1963 mL 0.9816 mL 1.9632 mL

*

请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液；一旦配成溶液，请分装保存，避免反复冻融造成的产品失效。
储备液的保存方式和期限：-80°C, 6 months; -20°C, 1 month。-80°C 储存时，请在 6 个月内使用，-20°C 储存时，请在 1 个月内使用。

In Vivo:

请根据您的实验动物和给药方式选择适当的溶解方案。以下溶解方案都请先按照 In Vitro 方式配制澄清的储备液，再依次添加助溶剂：

——为保证实验结果的可靠性，澄清的储备液可以根据储存条件，适当保存；体内实验的工作液，建议您现用现配，当天使用； 以下溶剂前显示的百
分比是指该溶剂在您配制终溶液中的体积占比；如在配制过程中出现沉淀、析出现象，可以通过加热和/或超声的方式助溶

  • 1.

    请依序添加每种溶剂：;10% DMSO ;; 40% PEG300 ;; 5% Tween-80 ;; 45% saline

    Solubility: ≥ 1.25 mg/mL (2.45 mM); Clear solution

    此方案可获得 ≥ 1.25 mg/mL (2.45 mM，饱和度未知) 的澄清溶液。

    以 1 mL 工作液为例，取 100 μL 12.5 mg/mL 的澄清 DMSO 储备液加到 400 μL PEG300 中，混合均匀；向上述体系中加入50 μL Tween-80，混合均匀；然后继续加入 450 μL生理盐水定容至 1 mL。

    将 0.9 g 氯化钠，完全溶解于 100 mL ddH₂O 中，得到澄清透明的生理盐水溶液

  • 2.

    请依序添加每种溶剂：;10% DMSO ;; 90% (20% SBE-β-CD in saline)

    Solubility: ≥ 1.25 mg/mL (2.45 mM); Clear solution

    此方案可获得 ≥ 1.25 mg/mL (2.45 mM，饱和度未知) 的澄清溶液。

    以 1 mL 工作液为例，取 100 μL 12.5 mg/mL 的澄清 DMSO 储备液加到 900 μL 20% 的 SBE-β-CD 生理盐水水溶液中，混合均匀。

    将 2 g 磺丁基醚 β-环糊精加入 5 mL 生理盐水中，再用生理盐水定容至 10 mL，完全溶解，澄清透明
*以上所有助溶剂都可在 MCE 网站选购。
参考文献

  • [1]. Angel Orte, et al. Absorption and emission study of 2′,7′-difluorofluorescein and its excited-state buffer-mediated proton exchange reactions. J Phys Chem A. 2005 Feb 10;109(5):734-47.

荧光染料OG 488, SE(Synonyms: Oregon green 488 succinimidyl ester)

发表于由

荧光染料Dye Reagents OG 488, SE;(Synonyms: Oregon green 488 succinimidyl ester) 纯度: 95.08%

OG 488, SE (Oregon green 488 succinimidyl ester) 是一种荧光 pH 指示剂，是一种基于荧光素的染料，在生物化学和神经科学领域具有许多应用。

OG 488, SE(Synonyms: Oregon green 488 succinimidyl ester)

OG 488, SE Chemical Structure

CAS No. : 198139-51-4

规格 价格 是否有货 数量
5 mg ￥2300 In-stock
10 mg ￥3600 In-stock
25 mg ￥7200 询价
50 mg ; 询价 ;
100 mg ; 询价 ;

* Please select Quantity before adding items.

OG 488, SE 相关产品

bull;相关化合物库:

  • Bioactive Compound Library Plus

生物活性

OG 488, SE (Oregon green 488 succinimidyl ester), a fluorescent pH indicator, has many applications in biochemistry and neurosciences[1].

分子量

509.37

Formula

C25H13F2NO9
CAS 号

198139-51-4
运输条件

Room temperature in continental US; may vary elsewhere.
储存方式
Powder -20deg;C 3 years
4deg;C 2 years
In solvent -80deg;C 6 months
-20deg;C 1 month
溶解性数据
In Vitro:;

DMSO : 125 mg/mL (245.40 mM; Need ultrasonic)

配制储备液
浓度 溶剂体积 质量 1 mg 5 mg 10 mg
1 mM 1.9632 mL 9.8160 mL 19.6321 mL
5 mM 0.3926 mL 1.9632 mL 3.9264 mL
10 mM 0.1963 mL 0.9816 mL 1.9632 mL

*

请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液；一旦配成溶液，请分装保存，避免反复冻融造成的产品失效。
储备液的保存方式和期限：-80°C, 6 months; -20°C, 1 month。-80°C 储存时，请在 6 个月内使用，-20°C 储存时，请在 1 个月内使用。

In Vivo:

请根据您的实验动物和给药方式选择适当的溶解方案。以下溶解方案都请先按照 In Vitro 方式配制澄清的储备液，再依次添加助溶剂：

——为保证实验结果的可靠性，澄清的储备液可以根据储存条件，适当保存；体内实验的工作液，建议您现用现配，当天使用； 以下溶剂前显示的百
分比是指该溶剂在您配制终溶液中的体积占比；如在配制过程中出现沉淀、析出现象，可以通过加热和/或超声的方式助溶

  • 1.

    请依序添加每种溶剂：;10% DMSO ;; 40% PEG300 ;; 5% Tween-80 ;; 45% saline

    Solubility: ≥ 1.25 mg/mL (2.45 mM); Clear solution

    此方案可获得 ≥ 1.25 mg/mL (2.45 mM，饱和度未知) 的澄清溶液。

    以 1 mL 工作液为例，取 100 μL 12.5 mg/mL 的澄清 DMSO 储备液加到 400 μL PEG300 中，混合均匀；向上述体系中加入50 μL Tween-80，混合均匀；然后继续加入 450 μL生理盐水定容至 1 mL。

    将 0.9 g 氯化钠，完全溶解于 100 mL ddH₂O 中，得到澄清透明的生理盐水溶液

  • 2.

    请依序添加每种溶剂：;10% DMSO ;; 90% (20% SBE-β-CD in saline)

    Solubility: ≥ 1.25 mg/mL (2.45 mM); Clear solution

    此方案可获得 ≥ 1.25 mg/mL (2.45 mM，饱和度未知) 的澄清溶液。

    以 1 mL 工作液为例，取 100 μL 12.5 mg/mL 的澄清 DMSO 储备液加到 900 μL 20% 的 SBE-β-CD 生理盐水水溶液中，混合均匀。

    将 2 g 磺丁基醚 β-环糊精加入 5 mL 生理盐水中，再用生理盐水定容至 10 mL，完全溶解，澄清透明
*以上所有助溶剂都可在 MCE 网站选购。
参考文献

  • [1]. Angel Orte, et al. Absorption and emission study of 2′,7′-difluorofluorescein and its excited-state buffer-mediated proton exchange reactions. J Phys Chem A. 2005 Feb 10;109(5):734-47.

荧光染料6-TAMRA-SE(Synonyms: 6-TAMRA-NHS ester 6-Carboxytetramethylrhodamine N-succinimidyl ester)

发表于由

荧光染料Dye Reagents 6-TAMRA-SE;(Synonyms: 6-TAMRA-NHS ester; 6-Carboxytetramethylrhodamine N-succinimidyl ester) 纯度: ge;95.0%

6-TAMRA-SE (6-TAMRA-NHS ester) 是一种带有胺反应基的荧光染料。6-TAMRA-SE 是用于 DNA 自动测序的传统荧光团之一。

6-TAMRA-SEamp;;(Synonyms: 6-TAMRA-NHS ester; 6-Carboxytetramethylrhodamine N-succinimidyl ester)

6-TAMRA-SE Chemical Structure

CAS No. : 150810-69-8

规格 价格 是否有货 数量
10;mM;*;1 mL in DMSO ￥1625 In-stock
1 mg ￥600 In-stock
5 mg ￥1400 In-stock
10 mg ￥2000 In-stock
50 mg ￥7500 In-stock
100 mg ; 询价 ;
200 mg ; 询价 ;

* Please select Quantity before adding items.

6-TAMRA-SE 相关产品

bull;相关化合物库:

  • Bioactive Compound Library Plus

生物活性

6-TAMRA-SE (6-TAMRA-NHS ester) is a fluorescent dye carrying the amine reactive group. 6-TAMRA-SE is one of the traditional fluorophores used for automated DNA sequencing[1][2][3].

体外研究
(In Vitro)

6-TAMRA-SE (6-TAMRA-NHS ester) is an amine-reactive form of tetramethylrhodamine. 6-TAMRA-SE (soluble in DMF and DMSO, absorption=546, emission=576 measured in MeOH ϵ=9.5×104ϵ=9.5×104)[1][3].

MCE has not independently confirmed the accuracy of these methods. They are for reference only.
分子量

527.52

Formula

C29H25N3O7
CAS 号

150810-69-8
中文名称

6-羧基四甲基罗丹明琥珀酰亚胺酯
运输条件

Room temperature in continental US; may vary elsewhere.
储存方式

-20deg;C, sealed storage, away from moisture and light

*In solvent : -80deg;C, 6 months; -20deg;C, 1 month (sealed storage, away from moisture and light)
溶解性数据
In Vitro:;

DMSO : 100 mg/mL (189.57 mM; Need ultrasonic)

配制储备液
浓度 溶剂体积 质量 1 mg 5 mg 10 mg
1 mM 1.8957 mL 9.4783 mL 18.9566 mL
5 mM 0.3791 mL 1.8957 mL 3.7913 mL
10 mM 0.1896 mL 0.9478 mL 1.8957 mL

*

请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液；一旦配成溶液，请分装保存，避免反复冻融造成的产品失效。
储备液的保存方式和期限：-80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light)。-80°C 储存时，请在 6 个月内使用，-20°C 储存时，请在 1 个月内使用。

In Vivo:

请根据您的实验动物和给药方式选择适当的溶解方案。以下溶解方案都请先按照 In Vitro 方式配制澄清的储备液，再依次添加助溶剂：

——为保证实验结果的可靠性，澄清的储备液可以根据储存条件，适当保存；体内实验的工作液，建议您现用现配，当天使用； 以下溶剂前显示的百
分比是指该溶剂在您配制终溶液中的体积占比；如在配制过程中出现沉淀、析出现象，可以通过加热和/或超声的方式助溶

  • 1.

    请依序添加每种溶剂：;10% DMSO ;; 40% PEG300 ;; 5% Tween-80 ;; 45% saline

    Solubility: 2.5 mg/mL (4.74 mM); Suspended solution; Need ultrasonic

    此方案可获得 2.5 mg/mL (4.74 mM) 的均匀悬浊液，悬浊液可用于口服和腹腔注射。

    以 1 mL 工作液为例，取 100 μL 25.0 mg/mL 的澄清 DMSO 储备液加到 400 μL PEG300 中，混合均匀；向上述体系中加入50 μL Tween-80，混合均匀；然后继续加入 450 μL生理盐水定容至 1 mL。

    将 0.9 g 氯化钠，完全溶解于 100 mL ddH₂O 中，得到澄清透明的生理盐水溶液

*以上所有助溶剂都可在 MCE 网站选购。
参考文献

  • [1]. Wang Y, et al. RNA molecules that specifically and stoichiometrically bind aminoglycoside antibiotics with high affinities. Biochemistry. 1996 Sep 24;35(38):12338-46.

    [2]. Tessmar J, et al. Toward the development of biomimetic polymers by protein immobilization: PEGylation of insulin as a model reaction. Tissue Eng. 2004;10(3-4):441-453.

    [3]. Thomas TP, et al. Investigation of tumor cell targeting of a dendrimer nanoparticle using a double-clad optical fiber probe. J Biomed Opt. 2008;13(1):014024.

荧光染料6-TAMRA-SE(Synonyms: 6-TAMRA-NHS ester 6-Carboxytetramethylrhodamine N-succinimidyl ester)

发表于由

荧光染料Dye Reagents 6-TAMRA-SE;(Synonyms: 6-TAMRA-NHS ester; 6-Carboxytetramethylrhodamine N-succinimidyl ester) 纯度: ge;95.0%

6-TAMRA-SE (6-TAMRA-NHS ester) 是一种带有胺反应基的荧光染料。6-TAMRA-SE 是用于 DNA 自动测序的传统荧光团之一。

6-TAMRA-SE(Synonyms: 6-TAMRA-NHS ester; 6-Carboxytetramethylrhodamine N-succinimidyl ester)

6-TAMRA-SE Chemical Structure

CAS No. : 150810-69-8

规格 价格 是否有货 数量
10;mM;*;1 mL in DMSO ￥1625 In-stock
1 mg ￥600 In-stock
5 mg ￥1400 In-stock
10 mg ￥2000 In-stock
50 mg ￥7500 In-stock
100 mg ; 询价 ;
200 mg ; 询价 ;

* Please select Quantity before adding items.

6-TAMRA-SE 相关产品

bull;相关化合物库:

  • Bioactive Compound Library Plus

生物活性

6-TAMRA-SE (6-TAMRA-NHS ester) is a fluorescent dye carrying the amine reactive group. 6-TAMRA-SE is one of the traditional fluorophores used for automated DNA sequencing[1][2][3].

体外研究
(In Vitro)

6-TAMRA-SE (6-TAMRA-NHS ester) is an amine-reactive form of tetramethylrhodamine. 6-TAMRA-SE (soluble in DMF and DMSO, absorption=546, emission=576 measured in MeOH ϵ=9.5×104ϵ=9.5×104)[1][3].

MCE has not independently confirmed the accuracy of these methods. They are for reference only.
分子量

527.52

Formula

C29H25N3O7
CAS 号

150810-69-8
中文名称

6-羧基四甲基罗丹明琥珀酰亚胺酯
运输条件

Room temperature in continental US; may vary elsewhere.
储存方式

-20deg;C, sealed storage, away from moisture and light

*In solvent : -80deg;C, 6 months; -20deg;C, 1 month (sealed storage, away from moisture and light)
溶解性数据
In Vitro:;

DMSO : 100 mg/mL (189.57 mM; Need ultrasonic)

配制储备液
浓度 溶剂体积 质量 1 mg 5 mg 10 mg
1 mM 1.8957 mL 9.4783 mL 18.9566 mL
5 mM 0.3791 mL 1.8957 mL 3.7913 mL
10 mM 0.1896 mL 0.9478 mL 1.8957 mL

*

请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液；一旦配成溶液，请分装保存，避免反复冻融造成的产品失效。
储备液的保存方式和期限：-80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light)。-80°C 储存时，请在 6 个月内使用，-20°C 储存时，请在 1 个月内使用。

In Vivo:

请根据您的实验动物和给药方式选择适当的溶解方案。以下溶解方案都请先按照 In Vitro 方式配制澄清的储备液，再依次添加助溶剂：

——为保证实验结果的可靠性，澄清的储备液可以根据储存条件，适当保存；体内实验的工作液，建议您现用现配，当天使用； 以下溶剂前显示的百
分比是指该溶剂在您配制终溶液中的体积占比；如在配制过程中出现沉淀、析出现象，可以通过加热和/或超声的方式助溶

  • 1.

    请依序添加每种溶剂：;10% DMSO ;; 40% PEG300 ;; 5% Tween-80 ;; 45% saline

    Solubility: 2.5 mg/mL (4.74 mM); Suspended solution; Need ultrasonic

    此方案可获得 2.5 mg/mL (4.74 mM) 的均匀悬浊液，悬浊液可用于口服和腹腔注射。

    以 1 mL 工作液为例，取 100 μL 25.0 mg/mL 的澄清 DMSO 储备液加到 400 μL PEG300 中，混合均匀；向上述体系中加入50 μL Tween-80，混合均匀；然后继续加入 450 μL生理盐水定容至 1 mL。

    将 0.9 g 氯化钠，完全溶解于 100 mL ddH₂O 中，得到澄清透明的生理盐水溶液

*以上所有助溶剂都可在 MCE 网站选购。
参考文献

  • [1]. Wang Y, et al. RNA molecules that specifically and stoichiometrically bind aminoglycoside antibiotics with high affinities. Biochemistry. 1996 Sep 24;35(38):12338-46.

    [2]. Tessmar J, et al. Toward the development of biomimetic polymers by protein immobilization: PEGylation of insulin as a model reaction. Tissue Eng. 2004;10(3-4):441-453.

    [3]. Thomas TP, et al. Investigation of tumor cell targeting of a dendrimer nanoparticle using a double-clad optical fiber probe. J Biomed Opt. 2008;13(1):014024.