Schisandrin (Schizandrin), a dibenzocyclooctadiene lignan, is isolated from the fruit of Schisandra chinensis Baill. Schisandrin exhibits antioxidant, hepatoprotective, anti-cancer and anti-inflammatory activities. Schisandrin also can reverses memory impairment in rats[1][2][3].
体外研究 (In Vitro)
Schisandrin (10-100 μM; pretreated for 2 h) inhibits LPS-stimulated NO production, iNOS protein and mRNA expression in a dose-dependent manner in RAW 264.7 cells[1]. Schisandrin (25-100 μM; pretreated for 2 h) inhibits prostaglandin E2 production, COX-2 protein and mRNA expression in LPS-stimulated RAW 264.7 macrophages[1]. Schisandrin inhibits the SK-HEP-1, SNU-638, and T47D cells proliferation, with IC50s of 42.0, 53.1, and 40.0 μM, respectively[3]. Schisandrin (10-60 μM; 48 h) enhances Doxorubicin -induced apoptosis and selectively reverses MCF-7/DOX resistance[4].
MCE has not independently confirmed the accuracy of these methods. They are for reference only.
Cell Viability Assay[1]
Cell Line:
RAW 264.7 macrophages
Concentration:
12.5, 25, 50, 100 μM
Incubation Time:
Pretreated for 2 h and then incubated with LPS (1 μg/mL) for 18 h
Result:
Markedly decreased iNOS protein expression a dose-dependent manner. Significantly inhibited COX-2 protein expression.
体内研究 (In Vivo)
Schisandrin (10-100 mg/kg; a single i.p.) inhibits acetic acid-induced peritoneal vascular permeability and reduces the carrageenan-induced paw edema of mice[1]. Schisandrin (1-10 mg/kg; a single p.o.) significantly reverses the Scopolamine-induced impairment of spatial memory and passive avoidance response, and enhances tremors induced by Oxotremorine in rats[2].
MCE has not independently confirmed the accuracy of these methods. They are for reference only.
Animal Model:
Male ICR mice (4-weeks) were treated with λ-carrageenan by intraperitoneal injection into the right hind paw[1]
Dosage:
100, 200 mg/kg
Administration:
A single i.p.
Result:
Inhibited paw edema by 33.43 % (100 mg/kg) and 57.38 % (200 mg/kg) at 3 h.
分子量
432.51
Formula
C24H32O7
CAS 号
7432-28-2
中文名称
五味子素；五味子醇甲
运输条件
Room temperature in continental US; may vary elsewhere.
[1]. Guo LY, et, al. Anti-inflammatory effects of schisandrin isolated from the fruit of Schisandra chinensis Baill. Eur J Pharmacol. 2008 Sep 4;591(1-3):293-9.
[2]. Egashira N, et, al. Schizandrin reverses memory impairment in rats. Phytother Res. 2008 Jan;22(1):49-52.
[3]. Min HY, et, al. Antiproliferative effects of dibenzocyclooctadiene lignans isolated from Schisandra chinensis in human cancer cells. Bioorg Med Chem Lett. 2008 Jan 15;18(2):523-6.
[4]. Zhang ZL, et, al. Schisandrin A reverses doxorubicin-resistant human breast cancer cell line by the inhibition of P65 and Stat3 phosphorylation. Breast Cancer. 2018 Mar;25(2):233-242.
Schisandrin C (Schizandrin-C) 是从五味子中分离得到的一种植物化学木脂素 (lignan)。Schisandrin C 具有多种生物活性，包括抗癌、抗炎和抗氧化作用。Schisandrin C 可用于癌症、阿尔茨海默病、肝病的研究。Schisandrin C 诱导细胞凋亡 (apoptosis)。
Schisandrin C (Schizandrin-C) is a phytochemical lignan isolated from Schizandra chinensis[1]. Schisandrin C has diverse biological activities, including anticancer, anti-inflammatory and antioxidant effects. Schisandrin C can be used for cancer, alzheimer’s disease, and liver diseases research[2][3]. Schisandrin C induces cell apoptosis[1].
体外研究 (In Vitro)
Schisandrin C (25-100 μM; 48 hours) down-regulates expression levels of Bcl-2, Bcl-xL and a concomitant degradation of poly(ADP-ribose) polymerase (PARP). It also activates caspase-3 and -9 in U937 cells[2].Schisandrin C (25-100 μM; 48 hours) induces apoptosis in a dose-dependent manner. And the fragmentation of genomic DNA is also increased in U937 cells[1].Schisandrin C (25-100 μM; 72 hours) induces G1 arrest, it down-regulates cyclin D1, cyclin E, cyclin-dependent kinase (Cdk) 4 and E2Fs expression, and also exhibits inhibition of pRB, and up-regulation of the Cdk inhibitor p21(WAF1/CIP1)[1].
MCE has not independently confirmed the accuracy of these methods. They are for reference only.
Western Blot Analysis[1]
Cell Line:
U937 cells
Concentration:
25 μM,50 μM,100 μM
Incubation Time:
48 hours
Result:
Decreased the expression of apoptosis related proteins.
Apoptosis Analysis[1]
Cell Line:
U937 cells
Concentration:
25 μM,50 μM,100 μM
Incubation Time:
48 hours
Result:
Induced cell apoptosis.
Cell Cycle Analysis[1]
Cell Line:
U937 cells
Concentration:
25 μM,50 μM,100 μM
Incubation Time:
48 hours
Result:
Induced growth inhibition and G1 arrest of U937 cells.
体内研究 (In Vivo)
Schisandrin C (lateral ventricle injection (i.c.v.); 15-150 μg/kg; 5 days) reduces Aβ1-42-induced memory deficits in the Y-maze test. Neurons in the hippocampus of SCH-C (15 μg/kg)-treated group returned to normal level, and and SCH-C group (150 μg/kg) has slight neuroprotective effects Aβ1-42-induced group. SCH-C (15 μg/kg) recoveres the activities of SOD and GSHPx and the ratios of GSH, decreases the levels of ChEtotal in the brain of the Aβ1–42-induced amnesic mice simultaneously[3].
MCE has not independently confirmed the accuracy of these methods. They are for reference only.
Animal Model:
Aβ1-42-induced Alzheimer’s disease mice[3]
Dosage:
15-150 μg/kg
Administration:
Lateral ventricle injection (i.c.v.); 15-150 μg/kg; 5 days
Result:
Exhibited potent neuroprotective effects linking to anti-ChEtotal activities and anti-oxidative mechanisms in Aβ1-42-induced amnestic mice.
分子量
384.42
Formula
C22H24O6
CAS 号
61301-33-5
中文名称
五味子丙素
运输条件
Room temperature in continental US; may vary elsewhere.
[1]. Park C, et al. Induction of G1 arrest and apoptosis by schisandrin C isolated from Schizandra chinensis Baill in human leukemia U937 cells. Int J Mol Med. 2009 Oct;24(4):495-502.
[2]. Oh SY, et al. Anti-inflammatory effects of gomisin N, gomisin J, and schisandrin C isolated from the fruit of Schisandra chinensis. Biosci Biotechnol Biochem. 2010;74(2):285-91.
[3]. Xin Mao, et al.Schisandrin C Ameliorates Learning and Memory Deficits by Aβ 1-42 -induced Oxidative Stress and Neurotoxicity in Mice. Phytother Res. 2015 Sep;29(9):1373-1380.
