标签归档：rhodamine

Ubiquitin (human) (rec.) (Rhodamine 110) 泛素（人）（rec.）（罗丹明110） 品牌：South Bay

发表于由

Ubiquitin (human) (rec.) (Rhodamine 110)

泛素（人）（rec.）（罗丹明110）

品牌：South Bay
CAS No.：
储存条件：-80°C
纯度：>99% (LCMS)
产品编号

(生产商编号)

 等级 规格 运输包装 零售价(RMB) 库存情况 参考值

SBB-PS0001-C050

 – 50 µg – 4,000.00 – –


* 干冰运输、大包装及大批量的产品需酌情添加运输费用


* 零售价、促销产品折扣、运输费用、库存情况、产品及包装规格可能因各种原因有所变动，恕不另行通知，确切详情请联系上海金畔生物科技有限公司。

NEDD8 (human) (rec.) (Rhodamine 110) 神经前体细胞下调蛋白（人）（rec.）（罗丹明110） 品牌：South Bay

发表于由

NEDD8 (human) (rec.) (Rhodamine 110)

神经前体细胞下调蛋白（人）（rec.）（罗丹明110）

品牌：South Bay
CAS No.：
储存条件：-80°C
纯度：>97% (LCMS)
产品编号

(生产商编号)

 等级 规格 运输包装 零售价(RMB) 库存情况 参考值

SBB-PS0003-C050

 – 50 µg – 4,910.00 – –


* 干冰运输、大包装及大批量的产品需酌情添加运输费用


* 零售价、促销产品折扣、运输费用、库存情况、产品及包装规格可能因各种原因有所变动，恕不另行通知，确切详情请联系上海金畔生物科技有限公司。

Phalloidin, Rhodamine X conjugated 鬼笔环肽，罗丹明X缀合 品牌：FUJIFILM Wako

发表于由

Phalloidin, Rhodamine X conjugated

鬼笔环肽，罗丹明X缀合

品牌：FUJIFILM Wako
CAS No.：
储存条件：-20℃
纯度：–
产品编号

(生产商编号)

 等级 规格 运输包装 零售价(RMB) 库存情况 参考值

165-21641

 for Cellbiology 300tests – 咨询 – –


* 干冰运输、大包装及大批量的产品需酌情添加运输费用


* 零售价、促销产品折扣、运输费用、库存情况、产品及包装规格可能因各种原因有所变动，恕不另行通知，确切详情请联系上海金畔生物科技有限公司。

SUMO1 (human) (rec.) (Rhodamine 110) 小泛蛋白相关调节剂1（人）（rec.）（罗丹明110） 品牌：South Bay

发表于由

SUMO1 (human) (rec.) (Rhodamine 110)

小泛蛋白相关调节剂1（人）（rec.）（罗丹明110）

品牌：South Bay
CAS No.：
储存条件：-80°C
纯度：>97% (LCMS)
产品编号

(生产商编号)

 等级 规格 运输包装 零售价(RMB) 库存情况 参考值

SBB-PS0028-C050

 – 50 µg – 4,910.00 – –


* 干冰运输、大包装及大批量的产品需酌情添加运输费用


* 零售价、促销产品折扣、运输费用、库存情况、产品及包装规格可能因各种原因有所变动，恕不另行通知，确切详情请联系上海金畔生物科技有限公司。

SUMO2 (human) (rec.) (Rhodamine 110) 小泛蛋白相关调节剂2（人）（rec.）（罗丹明110） 品牌：South Bay

发表于由

SUMO2 (human) (rec.) (Rhodamine 110)

小泛蛋白相关调节剂2（人）（rec.）（罗丹明110）

品牌：South Bay
CAS No.：
储存条件：-80°C
纯度：>97% (LCMS)
产品编号

(生产商编号)

 等级 规格 运输包装 零售价(RMB) 库存情况 参考值

SBB-PS0029-C050

 – 50 µg – 4,910.00 – –


* 干冰运输、大包装及大批量的产品需酌情添加运输费用


* 零售价、促销产品折扣、运输费用、库存情况、产品及包装规格可能因各种原因有所变动，恕不另行通知，确切详情请联系上海金畔生物科技有限公司。

荧光染料Rhodamine 110(Synonyms: 罗丹明110 Rhodamine 110 chloride RH110)

发表于由

荧光染料Dye Reagents Rhodamine 110;(Synonyms: 罗丹明110; Rhodamine 110 chloride; RH110) 纯度: 98.65%

Rhodamine 110 是一种灵敏和选择性的底物 (substrate)，用于测定溶液或活细胞内的蛋白酶。激发波长为 498 nm，发射波长为 521 nm。

Rhodamine 110amp;;(Synonyms: 罗丹明110; Rhodamine 110 chloride; RH110)

Rhodamine 110 Chemical Structure

CAS No. : 13558-31-1

规格 价格 是否有货 数量
Free Sample (0.1-0.5 mg) ; Apply now ;
10;mM;*;1 mL in DMSO ￥550 In-stock
50 mg ￥500 In-stock
100 mg ￥700 In-stock
200 mg ; 询价 ;
500 mg ; 询价 ;

* Please select Quantity before adding items.

Rhodamine 110 相关产品

bull;相关化合物库:

  • Bioactive Compound Library Plus
  • Anti-Blood Cancer Compound Library

生物活性

Rhodamine 110 is a sensitive and selective substrate for assaying proteinases in solution or inside living cells. The excitation wavelength is 498 nm and the emission wavelength is 521 nm[1].

体外研究
(In Vitro)

Rhodamine 110 accumulates in mitochondria in a cationic form, which alters the pH in this cellular compartment. Rhodamine 110 accumulates in human lymphoblastoid cells and Friend leukemia cells. No cytotoxicity to human lymphoblastoid cells is observed below 10 μM, but Rhodamine 110 causes Friend leukemia cells to die at a concentrations above 100 μM[1].

MCE has not independently confirmed the accuracy of these methods. They are for reference only.
体内研究
(In Vivo)

Rhodamine 110 is less toxic than the parent molecule based on the intravenous LD50 acute toxicity values of 89.5 mg/kg and 140.0 mg/kg for Rhodamine B and Rhodamine 110, respectively. Both molecules induce liver and kidney enlargement after ingestion, and male rats show more significant increases than female rats after Rhodamine 110 exposure. In addition, testis weight increased in male rats dosed with Rhodamine 110. The pharmacokinetics of Rhodamine 110 are assessed following oral administration at two dosages (3 and 10 mg/kg) and intravenous administration at one dosage (3 mg/kg). Pharmacokinetic parameters are calculated using an extravascular input and IV-bolus input, noncompartmental model analysis conducted with WinNonlin Standard Edition. The pharmacokinetic parameters of Rhodamine 110 indicates that the maximum plasma concentrations (Cmax) of the two oral dosages are 283.4 and 657.0 ng/mL, which are reached at 140 and 210 min, respectively. This indicates that Rhodamine 110 absorption is not rapid after ingestion, as it took over 2 h to be absorbed from the intestines into the blood. The areas under the concentration–time curves (AUCs) for the two dosages are 138.1±20.3 and 444.0±170.8 h ng/mL. The pharmacokinetic data demonstrate that the AUC is proportional to the administered oral dose of Rhodamine 110 (3 mg/kg and 10 mg/kg). Furthermore, the clearance (Cl) of the two orally administered doses is 7.94 and 8.61 mL/min/kg, respectively[1].

MCE has not independently confirmed the accuracy of these methods. They are for reference only.
分子量

366.80

Formula

C20H15ClN2O3
CAS 号

13558-31-1
中文名称

罗丹明110
运输条件

Room temperature in continental US; may vary elsewhere.
储存方式

4deg;C, sealed storage, away from moisture and light

*In solvent : -80deg;C, 6 months; -20deg;C, 1 month (sealed storage, away from moisture and light)
溶解性数据
In Vitro:;

DMSO : 30 mg/mL (81.79 mM; Need ultrasonic and warming)

H2O : < 0.1 mg/mL (insoluble)

配制储备液
浓度 溶剂体积 质量 1 mg 5 mg 10 mg
1 mM 2.7263 mL 13.6314 mL 27.2628 mL
5 mM 0.5453 mL 2.7263 mL 5.4526 mL
10 mM 0.2726 mL 1.3631 mL 2.7263 mL

*

请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液；一旦配成溶液，请分装保存，避免反复冻融造成的产品失效。
储备液的保存方式和期限：-80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light)。-80°C 储存时，请在 6 个月内使用，-20°C 储存时，请在 1 个月内使用。
参考文献

  • [1]. Jiang SH, et al. Pharmacokinetics of Rhodamine 110 and Its Organ Distribution in Rats. J Agric Food Chem. 2017 Sep 6;65(35):7797-7804.
Animal Administration
[1]

Rats[1]
Adult male Sprague-Dawley rats (230±20 g) are used. After the rats recovered, Rhodamine 110 in polyethylene glycol 400 (0.3 and 1 mg/mL) is administered at 3 mg/kg and 10 mg/kg by oral gavage. Blood samples (150 μL) are collected from the right jugular vein 5, 15, 30, 60, 120, 180, 240, 300, 360, 480, and 720 min after drug administration. After each sampling, 100 μL of normal saline is administered via catheter to compensate for the loss of body fluid, and a 50 μL heparin solution (20 IU heparin/mL normal saline) is provided to prevent coagulation. Blood samples are centrifuged at 16,000g for 10 min at 4 °C to obtain plasma, which is stored at -20 °C until analysis. After surgery, Rhodamine 110 in polyethylene glycol 400 (1 mg/mL) is administered intravenously to rats at 3 mg/kg (n=6). A 150 μL blood sample is collected from the right jugular vein 5, 15, 30, 60, 120, 180, 240, 300, 360, 480, and 720 min after drug administration. Then, 100 μL of normal saline is administered via the right jugular vein to compensate for body fluid loss, and 50 μL of a heparin solution (20 IU heparin/mL normal saline) is provided to prevent blood clotting.

MCE has not independently confirmed the accuracy of these methods. They are for reference only.
参考文献

  • [1]. Jiang SH, et al. Pharmacokinetics of Rhodamine 110 and Its Organ Distribution in Rats. J Agric Food Chem. 2017 Sep 6;65(35):7797-7804.

荧光染料Rhodamine 110(Synonyms: 罗丹明110 Rhodamine 110 chloride RH110)

发表于由

荧光染料Dye Reagents Rhodamine 110;(Synonyms: 罗丹明110; Rhodamine 110 chloride; RH110) 纯度: 98.65%

Rhodamine 110 是一种灵敏和选择性的底物 (substrate)，用于测定溶液或活细胞内的蛋白酶。激发波长为 498 nm，发射波长为 521 nm。

Rhodamine 110(Synonyms: 罗丹明110; Rhodamine 110 chloride; RH110)

Rhodamine 110 Chemical Structure

CAS No. : 13558-31-1

规格 价格 是否有货 数量
Free Sample (0.1-0.5 mg) ; Apply now ;
10;mM;*;1 mL in DMSO ￥550 In-stock
50 mg ￥500 In-stock
100 mg ￥700 In-stock
200 mg ; 询价 ;
500 mg ; 询价 ;

* Please select Quantity before adding items.

Rhodamine 110 相关产品

bull;相关化合物库:

  • Bioactive Compound Library Plus
  • Anti-Blood Cancer Compound Library

生物活性

Rhodamine 110 is a sensitive and selective substrate for assaying proteinases in solution or inside living cells. The excitation wavelength is 498 nm and the emission wavelength is 521 nm[1].

体外研究
(In Vitro)

Rhodamine 110 accumulates in mitochondria in a cationic form, which alters the pH in this cellular compartment. Rhodamine 110 accumulates in human lymphoblastoid cells and Friend leukemia cells. No cytotoxicity to human lymphoblastoid cells is observed below 10 μM, but Rhodamine 110 causes Friend leukemia cells to die at a concentrations above 100 μM[1].

MCE has not independently confirmed the accuracy of these methods. They are for reference only.
体内研究
(In Vivo)

Rhodamine 110 is less toxic than the parent molecule based on the intravenous LD50 acute toxicity values of 89.5 mg/kg and 140.0 mg/kg for Rhodamine B and Rhodamine 110, respectively. Both molecules induce liver and kidney enlargement after ingestion, and male rats show more significant increases than female rats after Rhodamine 110 exposure. In addition, testis weight increased in male rats dosed with Rhodamine 110. The pharmacokinetics of Rhodamine 110 are assessed following oral administration at two dosages (3 and 10 mg/kg) and intravenous administration at one dosage (3 mg/kg). Pharmacokinetic parameters are calculated using an extravascular input and IV-bolus input, noncompartmental model analysis conducted with WinNonlin Standard Edition. The pharmacokinetic parameters of Rhodamine 110 indicates that the maximum plasma concentrations (Cmax) of the two oral dosages are 283.4 and 657.0 ng/mL, which are reached at 140 and 210 min, respectively. This indicates that Rhodamine 110 absorption is not rapid after ingestion, as it took over 2 h to be absorbed from the intestines into the blood. The areas under the concentration–time curves (AUCs) for the two dosages are 138.1±20.3 and 444.0±170.8 h ng/mL. The pharmacokinetic data demonstrate that the AUC is proportional to the administered oral dose of Rhodamine 110 (3 mg/kg and 10 mg/kg). Furthermore, the clearance (Cl) of the two orally administered doses is 7.94 and 8.61 mL/min/kg, respectively[1].

MCE has not independently confirmed the accuracy of these methods. They are for reference only.
分子量

366.80

Formula

C20H15ClN2O3
CAS 号

13558-31-1
中文名称

罗丹明110
运输条件

Room temperature in continental US; may vary elsewhere.
储存方式

4deg;C, sealed storage, away from moisture and light

*In solvent : -80deg;C, 6 months; -20deg;C, 1 month (sealed storage, away from moisture and light)
溶解性数据
In Vitro:;

DMSO : 30 mg/mL (81.79 mM; Need ultrasonic and warming)

H2O : < 0.1 mg/mL (insoluble)

配制储备液
浓度 溶剂体积 质量 1 mg 5 mg 10 mg
1 mM 2.7263 mL 13.6314 mL 27.2628 mL
5 mM 0.5453 mL 2.7263 mL 5.4526 mL
10 mM 0.2726 mL 1.3631 mL 2.7263 mL

*

请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液；一旦配成溶液，请分装保存，避免反复冻融造成的产品失效。
储备液的保存方式和期限：-80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light)。-80°C 储存时，请在 6 个月内使用，-20°C 储存时，请在 1 个月内使用。
参考文献

  • [1]. Jiang SH, et al. Pharmacokinetics of Rhodamine 110 and Its Organ Distribution in Rats. J Agric Food Chem. 2017 Sep 6;65(35):7797-7804.
Animal Administration
[1]

Rats[1]
Adult male Sprague-Dawley rats (230±20 g) are used. After the rats recovered, Rhodamine 110 in polyethylene glycol 400 (0.3 and 1 mg/mL) is administered at 3 mg/kg and 10 mg/kg by oral gavage. Blood samples (150 μL) are collected from the right jugular vein 5, 15, 30, 60, 120, 180, 240, 300, 360, 480, and 720 min after drug administration. After each sampling, 100 μL of normal saline is administered via catheter to compensate for the loss of body fluid, and a 50 μL heparin solution (20 IU heparin/mL normal saline) is provided to prevent coagulation. Blood samples are centrifuged at 16,000g for 10 min at 4 °C to obtain plasma, which is stored at -20 °C until analysis. After surgery, Rhodamine 110 in polyethylene glycol 400 (1 mg/mL) is administered intravenously to rats at 3 mg/kg (n=6). A 150 μL blood sample is collected from the right jugular vein 5, 15, 30, 60, 120, 180, 240, 300, 360, 480, and 720 min after drug administration. Then, 100 μL of normal saline is administered via the right jugular vein to compensate for body fluid loss, and 50 μL of a heparin solution (20 IU heparin/mL normal saline) is provided to prevent blood clotting.

MCE has not independently confirmed the accuracy of these methods. They are for reference only.
参考文献

  • [1]. Jiang SH, et al. Pharmacokinetics of Rhodamine 110 and Its Organ Distribution in Rats. J Agric Food Chem. 2017 Sep 6;65(35):7797-7804.

Rhodamine 123(Synonyms: 罗丹明123 RH-123 R-22420)

发表于由

Rhodamine 123;(Synonyms: 罗丹明123; RH-123; R-22420) 纯度: 99.73%

Rhodamine 123 (RH-123; R-22420) 是一种荧光染料（λex=503 nm, λem=527 nm）。

Rhodamine 123amp;;(Synonyms: 罗丹明123; RH-123; R-22420)

Rhodamine 123 Chemical Structure

CAS No. : 62669-70-9

规格 价格 是否有货 数量
10;mM;*;1 mL in DMSO ￥550 In-stock
5 mg ￥500 In-stock
10 mg ￥650 In-stock
25 mg ￥900 In-stock
50 mg ￥1500 In-stock
100 mg ￥2500 In-stock
200 mg ; 询价 ;
500 mg ; 询价 ;

* Please select Quantity before adding items.

Rhodamine 123 相关产品

bull;相关化合物库:

  • Bioactive Compound Library Plus
  • Anti-Cardiovascular Disease Compound Library

生物活性

Rhodamine 123 (RH-123; R-22420) is a fluorescent dye (λex=503 nm, λem=527 nm).

体外研究
(In Vitro)

The intensity of R123 fluorescence has a peak at concentration of 50 μM, and decreases to zero at higher concentrations due to self-quenching[1].

MCE has not independently confirmed the accuracy of these methods. They are for reference only.
分子量

380.82

Formula

C21H17ClN2O3
CAS 号

62669-70-9
中文名称

罗丹明123
运输条件

Room temperature in continental US; may vary elsewhere.
储存方式

4deg;C, sealed storage, away from moisture and light

*In solvent : -80deg;C, 6 months; -20deg;C, 1 month (sealed storage, away from moisture and light)
溶解性数据
In Vitro:;

DMSO : 100 mg/mL (262.59 mM; Need ultrasonic)

配制储备液
浓度 溶剂体积 质量 1 mg 5 mg 10 mg
1 mM 2.6259 mL 13.1296 mL 26.2591 mL
5 mM 0.5252 mL 2.6259 mL 5.2518 mL
10 mM 0.2626 mL 1.3130 mL 2.6259 mL

*

请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液；一旦配成溶液，请分装保存，避免反复冻融造成的产品失效。
储备液的保存方式和期限：-80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light)。-80°C 储存时，请在 6 个月内使用，-20°C 储存时，请在 1 个月内使用。
参考文献

  • [1]. M. Huang, et al. Mitochondrial Inner Membrane Electrophysiology Assessed by Rhodamine-123 Transport and Fluorescence. Ann Biomed Eng. 2007 Jul; 35(7): 1276–1285.
Kinase Assay
[1]

Measurements are made at room temperature with continuous stirring of the mitochondrial suspension using spectrophotometer equipped with a magnetic stirrer with fluorescent cation R123 as probe. Excitation and emission wavelengths are 503 nm and 527 nm, respectively. The incubation medium is the respiration buffer. R123 and sodium pyruvate are added to final concentrations of 50 nM and 10 mM, respectively. Isolated mitochondria maintain a steady membrane potential (±5%) throughout the duration of the recording[1].

MCE has not independently confirmed the accuracy of these methods. They are for reference only.
参考文献

  • [1]. M. Huang, et al. Mitochondrial Inner Membrane Electrophysiology Assessed by Rhodamine-123 Transport and Fluorescence. Ann Biomed Eng. 2007 Jul; 35(7): 1276–1285.

Rhodamine B(Synonyms: 罗丹明B Basic Violet 10 Brilliant Pink B Rhodamine O Tetraethylrhodamine)

发表于由

Rhodamine B;(Synonyms: 罗丹明B; Basic Violet 10; Brilliant Pink B; Rhodamine O; Tetraethylrhodamine) 纯度: 98.03%

Rhodamine B 是一种荧光染料，常用于染色纺织品、纸张、肥皂、皮革和药品。

Rhodamine Bamp;;(Synonyms: 罗丹明B; Basic Violet 10; Brilliant Pink B; Rhodamine O; Tetraethylrhodamine)

Rhodamine B Chemical Structure

CAS No. : 81-88-9

规格 价格 是否有货 数量
Free Sample (0.1-0.5 mg) ; Apply now ;
10;mM;*;1 mL in DMSO ￥550 In-stock
100 mg ￥500 In-stock
1 g ￥800 In-stock
5 g ; 询价 ;
10 g ; 询价 ;

* Please select Quantity before adding items.

Rhodamine B 相关产品

bull;相关化合物库:

  • FDA Approved amp; Pharmacopeial Drug Library
  • Inactive Ingredient Library
  • Tumorigenesis Related Compound Library
  • Bioactive Compound Library Plus

生物活性

Rhodamine B is a staining fluorescent dye, commonly used for dyeing textiles, paper, soap, leather, and drugs.

体外研究
(In Vitro)

Rhodamine B induces a concentration-dependent reduction of root meristem cells of A. cepa. mitotic activity. Rhodamine B induces various nuclear aberrations in A. cepa. root cells. In the 100 and 200 ppm rhodamine B groups, the frequencies of NBUDs and BN surpass those of the positive control (MMS) group. Rhodamine B-induced changes of H2O2 (a) and MDA (b) level increase in a concentration-dependent manner in A. cepa. roots[1].

MCE has not independently confirmed the accuracy of these methods. They are for reference only.
分子量

479.01

Formula

C28H31ClN2O3
CAS 号

81-88-9
中文名称

罗丹明B；玫瑰红B；蕊香红B；若丹明B；碱性玫瑰精
运输条件

Room temperature in continental US; may vary elsewhere.
储存方式

4deg;C, sealed storage, away from moisture and light

*In solvent : -80deg;C, 6 months; -20deg;C, 1 month (sealed storage, away from moisture and light)
溶解性数据
In Vitro:;

DMSO : 125 mg/mL (260.95 mM; Need ultrasonic)

H2O : 100 mg/mL (208.76 mM; Need ultrasonic)

配制储备液
浓度 溶剂体积 质量 1 mg 5 mg 10 mg
1 mM 2.0876 mL 10.4382 mL 20.8764 mL
5 mM 0.4175 mL 2.0876 mL 4.1753 mL
10 mM 0.2088 mL 1.0438 mL 2.0876 mL

*

请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液；一旦配成溶液，请分装保存，避免反复冻融造成的产品失效。
储备液的保存方式和期限：-80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light)。-80°C 储存时，请在 6 个月内使用，-20°C 储存时，请在 1 个月内使用。

In Vivo:

请根据您的实验动物和给药方式选择适当的溶解方案。以下溶解方案都请先按照 In Vitro 方式配制澄清的储备液，再依次添加助溶剂：

——为保证实验结果的可靠性，澄清的储备液可以根据储存条件，适当保存；体内实验的工作液，建议您现用现配，当天使用； 以下溶剂前显示的百
分比是指该溶剂在您配制终溶液中的体积占比；如在配制过程中出现沉淀、析出现象，可以通过加热和/或超声的方式助溶

  • 1.

    请依序添加每种溶剂：;10% DMSO ;; 40% PEG300 ;; 5% Tween-80 ;; 45% saline

    Solubility: ≥ 2.08 mg/mL (4.34 mM); Clear solution

    此方案可获得 ≥ 2.08 mg/mL (4.34 mM，饱和度未知) 的澄清溶液。

    以 1 mL 工作液为例，取 100 μL 20.8 mg/mL 的澄清 DMSO 储备液加到 400 μL PEG300 中，混合均匀；向上述体系中加入50 μL Tween-80，混合均匀；然后继续加入 450 μL生理盐水定容至 1 mL。

    将 0.9 g 氯化钠，完全溶解于 100 mL ddH₂O 中，得到澄清透明的生理盐水溶液

*以上所有助溶剂都可在 MCE 网站选购。
参考文献

  • [1]. Tan D, et al. Rhodamine B induces long nucleoplasmic bridges and other nuclear anomalies in Allium cepa root tip cells. Environ Sci Pollut Res Int. 2014 Mar;21(5):3363-70.

Rhodamine 6G(Synonyms: 罗丹明6G Basic Red 1)

发表于由

Rhodamine 6G;(Synonyms: 罗丹明6G; Basic Red 1) 纯度: ge;98.0%

Rhodamine 6G是罗丹明类似物，可用于Pgp外排测定试验中。它可用于MRP1-介导外排的动力学试验中，以及作为激光染料和具有线粒体探针的潜力。

Rhodamine 6Gamp;;(Synonyms: 罗丹明6G; Basic Red 1)

Rhodamine 6G Chemical Structure

CAS No. : 989-38-8

规格 价格 是否有货 数量
Free Sample (0.1-0.5 mg) ; Apply now ;
10;mM;*;1 mL in DMSO ￥550 In-stock
250 mg ￥500 In-stock
1 g ￥800 In-stock
5 g ￥1200 In-stock
10 g ; 询价 ;
50 g ; 询价 ;

* Please select Quantity before adding items.

Rhodamine 6G 相关产品

bull;相关化合物库:

  • FDA Approved amp; Pharmacopeial Drug Library
  • Clinical Compound Library Plus
  • Bioactive Compound Library Plus
  • Anti-Cancer Compound Library
  • Clinical Compound Library

生物活性

Rhodamine 6G is a rhodamine analog useful in Pgp efflux assays. It can be used in characterizing the kinetics of MRP1- mediated efflux, and as a laser dye and potential mitochondrial probe.

体外研究
(In Vitro)

Rhodamine 6G, also known as Rhodamine 590, is widely used as a lasing medium and as a fluorescence tracer. For use in dye lasers, it is dissolved in methanol, ethanol and a variety of other organic solvents. In environmental flow studies, the tracer medium is typically water. In ethanol, the absorption of rhodamine 6G ranges between 440 nm and 570 nm, with the peak at 530 nm. It is thus ideally suited for pumping by frequency doubled Nd:YAG lasers at 532 nm, copper vapor lasers at 511 nm, and argon-ion lasers at 514 nm. The resulting emission spectrum varies from about 510 nm to around 710 nm with the peak at 550 nm depending on the solvent and the dye concentration. However, the laser emission range is much narrower, from around 560 nm to 610 nm with the peak wavelength around 575 nm. Energy conversion efficiencies greater than 50% are achievable. In order to select the optimal solvent and dye concentration, good knowledge of their effects is a pre-requisite. Rhodamine 6G in DMSO shows a distinct behavior exhibiting only 41% of the fluorescence intensity of the methanol case with an 11 nm red-shifted wavelength. Relatively small changes of the fluorescence spectrum are observed for the different solvents; the highest fluorescence intensity is observed for methanol and lowest for DMSO. The shortest peak wavelength is found in methanol (568 nm) and the longest in DMSO (579 nm). Changing the dye concentration provides tunability between 550 nm in the dilute case and 620 nm at high concentration, at which point the fluorescence spectrum indicates the formation of rhodamine 6G aggregates[1]. Rhodamine 6G is a fluorescent dye capable of penetrating a living cell. Upon entering the cell, Rhodamine 6G binds to the inner membranes of mitochondria. Based on these observations, it has been proposed that Rhodamine dyes may be used for producing fluorescent images of mitochondria with low background noise and high resolution. Extremely low concentrations of Rhodamine 6G appear to selectively destroy malignant cells in culture, sparing the normal cell populations[2].

MCE has not independently confirmed the accuracy of these methods. They are for reference only.
体内研究
(In Vivo)

Melanoma-transplanted mice receiving Rhodamine 6G demonstrate prolonged survival, improved clinical parameters, inhibited tumor growth and metastases count, compared to their untreated counterparts. Twice-a-week 10-6M Rhodamine 6G regimen yield the most prominent results[2]. The Rhodamine-6G enters the circulatory system and labels leukocytes. It is possible to monitor changes in the interactions between leukocytes and the endothelium by determining the numbers of rolling and adhering leukocytes as well as the total flux of these cells[3].

MCE has not independently confirmed the accuracy of these methods. They are for reference only.
Clinical Trial

分子量

479.01

Formula

C28H31ClN2O3
CAS 号

989-38-8
中文名称

罗丹明6G
运输条件

Room temperature in continental US; may vary elsewhere.
储存方式

4deg;C, sealed storage, away from moisture and light

*In solvent : -80deg;C, 6 months; -20deg;C, 1 month (sealed storage, away from moisture and light)
溶解性数据
In Vitro:;

DMSO : 25 mg/mL (52.19 mM; Need ultrasonic)

H2O : 10 mg/mL (20.88 mM; ultrasonic and warming and heat to 60°C)

配制储备液
浓度 溶剂体积 质量 1 mg 5 mg 10 mg
1 mM 2.0876 mL 10.4382 mL 20.8764 mL
5 mM 0.4175 mL 2.0876 mL 4.1753 mL
10 mM 0.2088 mL 1.0438 mL 2.0876 mL

*

请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液；一旦配成溶液，请分装保存，避免反复冻融造成的产品失效。
储备液的保存方式和期限：-80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light)。-80°C 储存时，请在 6 个月内使用，-20°C 储存时，请在 1 个月内使用。

In Vivo:

请根据您的实验动物和给药方式选择适当的溶解方案。以下溶解方案都请先按照 In Vitro 方式配制澄清的储备液，再依次添加助溶剂：

——为保证实验结果的可靠性，澄清的储备液可以根据储存条件，适当保存；体内实验的工作液，建议您现用现配，当天使用； 以下溶剂前显示的百
分比是指该溶剂在您配制终溶液中的体积占比；如在配制过程中出现沉淀、析出现象，可以通过加热和/或超声的方式助溶

  • 1.

    请依序添加每种溶剂：;10% DMSO ;; 40% PEG300 ;; 5% Tween-80 ;; 45% saline

    Solubility: 2.5 mg/mL (5.22 mM); Suspended solution; Need ultrasonic

    此方案可获得 2.5 mg/mL (5.22 mM) 的均匀悬浊液，悬浊液可用于口服和腹腔注射。

    以 1 mL 工作液为例，取 100 μL 25.0 mg/mL 的澄清 DMSO 储备液加到 400 μL PEG300 中，混合均匀；向上述体系中加入50 μL Tween-80，混合均匀；然后继续加入 450 μL生理盐水定容至 1 mL。

    将 0.9 g 氯化钠，完全溶解于 100 mL ddH₂O 中，得到澄清透明的生理盐水溶液

  • 2.

    请依序添加每种溶剂：;10% DMSO ;; 90% (20% SBE-β-CD in saline)

    Solubility: 2.5 mg/mL (5.22 mM); Suspended solution; Need ultrasonic

    此方案可获得 2.5 mg/mL (5.22 mM) 的均匀悬浊液，悬浊液可用于口服和腹腔注射。

    以 1 mL 工作液为例，取 100 μL 25.0 mg/mL 的澄清 DMSO 储备液加到 900 μL 20% 的 SBE-β-CD 生理盐水水溶液中，混合均匀。

    将 2 g 磺丁基醚 β-环糊精加入 5 mL 生理盐水中，再用生理盐水定容至 10 mL，完全溶解，澄清透明
*以上所有助溶剂都可在 MCE 网站选购。
参考文献

  • [1]. Zehentbauer FM, et al. Fluorescence spectroscopy of Rhodamine 6G: concentration and solvent effects. Spectrochim Acta A Mol Biomol Spectrosc. 2014;121:147-51.

    [2]. Kutushov M, et al. Low concentrations of Rhodamine 6G selectively destroy tumor cells and improve survival of melanoma transplanted mice. Neoplasma. 2013;60(3):262-73.

    [3]. Jain RK, et al. Measuring leukocyte-endothelial interactions in mice. Cold Spring Harb Protoc. 2013 Jun 1;2013(6):561-3.
Cell Assay
[2]

Malignant cells and normal control cultures are seeded in equal (protein adjusted) cell amounts into 6-well tissue culture plates. The cells are pulsed with 25µCi/mL of 3H-Thymidine and immediately treated with Rhodamine 6G at the fixed concentration of 1 μM for 24h, 48h, 72h or 5 days (120h). Following 24h, 48h, 72h or 5 days, the excessive radioactive material is ished out with PBS. The cell samples are transferred into polystyrene vials containing 4 ml scintillation liquid, and their radioactivity counted in a β-counter. Total cell protein is assessed by Bradford’s assay[2].

MCE has not independently confirmed the accuracy of these methods. They are for reference only.
Animal Administration
[2]

Mice: C57Bl mice are implanted with B16-F10 melanoma and treated with Rhodamine 6G (1, 0.1, 0.01 μM) at different dosage/time regimens. Viability and proliferation of cultured tumor cells are analyzed[2].

MCE has not independently confirmed the accuracy of these methods. They are for reference only.
参考文献

  • [1]. Zehentbauer FM, et al. Fluorescence spectroscopy of Rhodamine 6G: concentration and solvent effects. Spectrochim Acta A Mol Biomol Spectrosc. 2014;121:147-51.

    [2]. Kutushov M, et al. Low concentrations of Rhodamine 6G selectively destroy tumor cells and improve survival of melanoma transplanted mice. Neoplasma. 2013;60(3):262-73.

    [3]. Jain RK, et al. Measuring leukocyte-endothelial interactions in mice. Cold Spring Harb Protoc. 2013 Jun 1;2013(6):561-3.