Recombinant Proteinase K;(Synonyms: 重组蛋白酶 K)
Recombinant Proteinase K 是一种丝氨酸蛋白酶 (serine protease),它能裂解脂肪族和芳香族氨基酸的羧基端肽键。Recombinant Proteinase K 可用于消化蛋白质和清除核酸制剂中的污染。
Recombinant Proteinase K Chemical Structure
规格 |
|
是否有货 |
|
100 mg |
; |
询价 |
; |
250 mg |
; |
询价 |
; |
500 mg |
; |
询价 |
; |
* Please select Quantity before adding items.
生物活性 |
Recombinant Proteinase K is a serine protease that cleaves the carboxy-terminated peptide bonds of aliphatic and aromatic amino acids. Recombinant Proteinase K can be used to digest proteins and remove contamination from nucleic acid preparations[1].
|
体外研究 (In Vitro) |
Proteinase K digestion[1]1. Cells are resuspended in lysis bufferand finally lysed on ice for 15 minutes.2. The crude lysates are obtained by centrifugation at 4°C for 15 minutes. Proteinase K is added to a 20 µl aliquot to get the final concentrations of 2, 4 and 8 µg/ml. Reactions are carried out at 37°C for 30 minutes.
MCE has not independently confirmed the accuracy of these methods. They are for reference only.
|
中文名称 |
|
运输条件 |
Room temperature in continental US; may vary elsewhere.
|
储存方式 |
Powder |
-80deg;C |
2 years |
|
-20deg;C |
1 year |
In solvent |
-80deg;C |
6 months |
|
-20deg;C |
1 month |
|
参考文献 |
|