标签归档：photo

荧光染料DiAzKs(Synonyms: H-L-Photo-lysine)

发表于由

荧光染料Dye Reagents DiAzKs;(Synonyms: H-L-Photo-lysine) 纯度: ge;98.0%

DiAzKs (H-L-Photo-lysine) 是一种含有重氮基的赖氨酸氨基酸，也是一种光交联剂。DiAzKs 可以选择性的插入到蛋白质中，并用于在体外和活细胞中交联蛋白质-蛋白质相互作用。DiAzKs 用作紫外光激活的光交联探针。

DiAzKs(Synonyms: H-L-Photo-lysine)

DiAzKs Chemical Structure

CAS No. : 1253643-88-7

规格 价格 是否有货 数量
5 mg ￥1650 In-stock
10 mg ￥2600 In-stock
50 mg ￥6500 In-stock
100 mg ; 询价 ;
200 mg ; 询价 ;

* Please select Quantity before adding items.

DiAzKs 相关产品

bull;相关化合物库:

  • Bioactive Compound Library Plus

生物活性

DiAzKs (H-L-Photo-lysine) is a diazirine-containing lysine amino acid and is a photo-cross-linker. DiAzKs can site-selective incorporated into proteins and is used to crosslink protein-protein interactions in vitro and in living cells. DiAzKs acts as a UV light-activated photo-crosslinking probe[1][2][3].

体外研究
(In Vitro)

Photo-lysine, which is readily incorporated into proteins by native mammalian translation machinery, can be used to capture and identify proteins that recognize lysine post-translational modifications (PTMs), including ‘readers’ and ‘erasers’ of histone modifications[2].

MCE has not independently confirmed the accuracy of these methods. They are for reference only.
分子量

272.30

Formula

C11H20N4O4
CAS 号

1253643-88-7
运输条件

Room temperature in continental US; may vary elsewhere.
储存方式
Powder -20deg;C 3 years
4deg;C 2 years
In solvent -80deg;C 6 months
-20deg;C 1 month
溶解性数据
In Vitro:;

H2O : 5 mg/mL (18.36 mM; Need ultrasonic)

DMSO : 2 mg/mL (7.34 mM; Need ultrasonic)

配制储备液
浓度 溶剂体积 质量 1 mg 5 mg 10 mg
1 mM 3.6724 mL 18.3621 mL 36.7242 mL
5 mM 0.7345 mL 3.6724 mL 7.3448 mL
10 mM 0.3672 mL 1.8362 mL 3.6724 mL

*

请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液；一旦配成溶液，请分装保存，避免反复冻融造成的产品失效。
储备液的保存方式和期限：-80°C, 6 months; -20°C, 1 month。-80°C 储存时，请在 6 个月内使用，-20°C 储存时，请在 1 个月内使用。

In Vivo:

请根据您的实验动物和给药方式选择适当的溶解方案。以下溶解方案都请先按照 In Vitro 方式配制澄清的储备液，再依次添加助溶剂：

——为保证实验结果的可靠性，澄清的储备液可以根据储存条件，适当保存；体内实验的工作液，建议您现用现配，当天使用； 以下溶剂前显示的百
分比是指该溶剂在您配制终溶液中的体积占比；如在配制过程中出现沉淀、析出现象，可以通过加热和/或超声的方式助溶

  • 1.

    请依序添加每种溶剂：;10% DMSO ;; 40% PEG300 ;; 5% Tween-80 ;; 45% saline

    Solubility: ≥ 0.67 mg/mL (2.46 mM); Clear solution

    此方案可获得 ≥ 0.67 mg/mL (2.46 mM，饱和度未知) 的澄清溶液。

    以 1 mL 工作液为例，取 100 μL 6.7 mg/mL 的澄清 DMSO 储备液加到 400 μL PEG300 中，混合均匀；向上述体系中加入50 μL Tween-80，混合均匀；然后继续加入 450 μL生理盐水定容至 1 mL。

    将 0.9 g 氯化钠，完全溶解于 100 mL ddH₂O 中，得到澄清透明的生理盐水溶液

  • 2.

    请依序添加每种溶剂：;10% DMSO ;; 90% (20% SBE-β-CD in saline)

    Solubility: ≥ 0.67 mg/mL (2.46 mM); Clear solution

    此方案可获得 ≥ 0.67 mg/mL (2.46 mM，饱和度未知) 的澄清溶液。

    以 1 mL 工作液为例，取 100 μL 6.7 mg/mL 的澄清 DMSO 储备液加到 900 μL 20% 的 SBE-β-CD 生理盐水水溶液中，混合均匀。

    将 2 g 磺丁基醚 β-环糊精加入 5 mL 生理盐水中，再用生理盐水定容至 10 mL，完全溶解，澄清透明
*以上所有助溶剂都可在 MCE 网站选购。
参考文献

  • [1]. Ai HW, et al. Probing protein-protein interactions with a genetically encoded photo-crosslinking amino acid. Chembiochem. 2011 Aug 16;12(12):1854-7.

    [2]. Chatterjee A, et al. Efficient viral delivery system for unnatural amino acid mutagenesis in mammalian cells. Proc Natl Acad Sci U S A. 2013 Jul 16;110(29):11803-8.

    [3]. Yang T, et al. Photo-lysine captures proteins that bind lysine post-translational modifications. Nat Chem Biol. 2016 Feb;12(2):70-2.

Photo-lysine hydrochloride

发表于由

生化分析试剂 Biochemical Assay Reagents
Photo-lysine hydrochloride;

Photo-lysine hydrochloride，一种新的基于赖氨酸光反应性的氨基酸，它捕获结合赖氨酸翻译后修饰的蛋白质。

Photo-lysine hydrochloride

Photo-lysine hydrochloride Chemical Structure

规格 价格 是否有货 数量
5 mg ￥12000 In-stock
10 mg ￥21600 In-stock
20 mg ￥36000 询价
50 mg ; 询价 ;
100 mg ; 询价 ;

* Please select Quantity before adding items.

生物活性

Photo-lysine hydrochloride, a new lysine-based photo-reactive amino acid, captures proteins that bind lysine post-translational modifications.

体外研究
(In Vitro)

Photo-lysine is designed and synthesized by incorporating a photo-cross-linker (diazirine) into the side chain of natural lysine. Photo-lysine, which is readily incorporated into proteins by native mammalian translation machinery, can be used to capture and identify proteins that recognize lysine post-translational modifications (PTMs), including ‘readers’ and ‘erasers’ of histone modifications. Photo-lysine can be incorporated into MDH2 and mediate photo-cross-linking to fix protein-protein interactions in cells. UV irradiation of cells in the presence of photo-lysine induced robust cross-linking of HSP90β and HSP60. Photo-lysine has higher efficiency than photo-leucine for photo-cross-linking of the two chaperone proteins. Photo-lysine enables capture of the heterodimer of proteins Ku70 and Ku80 within a protein complex. Photo-lysine enables identification of histone- and chromatin-binding proteins[1].

MCE has not independently confirmed the accuracy of these methods. They are for reference only.
分子量

245.11

Formula

C6H14Cl2N4O2
运输条件

Room temperature in continental US; may vary elsewhere.
储存方式

4deg;C, protect from light, stored under nitrogen

*In solvent : -80deg;C, 6 months; -20deg;C, 1 month (protect from light, stored under nitrogen)
溶解性数据
In Vitro:;

H2O : ≥ 40 mg/mL (163.19 mM)

* “≥” means soluble, but saturation unknown.

配制储备液
浓度 溶剂体积 质量 1 mg 5 mg 10 mg
1 mM 4.0798 mL 20.3990 mL 40.7980 mL
5 mM 0.8160 mL 4.0798 mL 8.1596 mL
10 mM 0.4080 mL 2.0399 mL 4.0798 mL

*

请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液；一旦配成溶液，请分装保存，避免反复冻融造成的产品失效。
储备液的保存方式和期限：-80°C, 6 months; -20°C, 1 month (protect from light, stored under nitrogen)。-80°C 储存时，请在 6 个月内使用，-20°C 储存时，请在 1 个月内使用。
参考文献

  • [1]. Yang T, et al. Photo-lysine captures proteins that bind lysine post-translational modifications. Nat Chem Biol. 2016 Feb;12(2):70-2.

Photo-lysine(Synonyms: Photo lysine)

发表于由

生化分析试剂 Biochemical Assay Reagents
Photo-lysine;(Synonyms: Photo lysine)

Photo-lysine，一种新的基于赖氨酸光反应性的氨基酸，它捕获结合赖氨酸翻译后修饰的蛋白质。

Photo-lysine(Synonyms: Photo lysine)

Photo-lysine Chemical Structure

CAS No. : 1863117-91-2

规格 价格 是否有货
5 mg ￥12000 询问价格 货期
10 mg ￥21600 询问价格 货期
20 mg ￥36000 询问价格 货期

* Please select Quantity before adding items.

Photo-lysine 的其他形式现货产品:

Photo-lysine hydrochloride

生物活性

Photo-lysine, a new lysine-based photo-reactive amino acid, captures proteins that bind lysine post-translational modifications.

体外研究
(In Vitro)

Photo-lysine is designed and synthesized by incorporating a photo-cross-linker (diazirine) into the side chain of natural lysine. Photo-lysine, which is readily incorporated into proteins by native mammalian translation machinery, can be used to capture and identify proteins that recognize lysine post-translational modifications (PTMs), including ‘readers’ and ‘erasers’ of histone modifications. Photo-lysine can be incorporated into MDH2 and mediate photo-cross-linking to fix protein-protein interactions in cells. UV irradiation of cells in the presence of photo-lysine induced robust cross-linking of HSP90β and HSP60. Photo-lysine has higher efficiency than photo-leucine for photo-cross-linking of the two chaperone proteins. Photo-lysine enables capture of the heterodimer of proteins Ku70 and Ku80 within a protein complex. Photo-lysine enables identification of histone- and chromatin-binding proteins[1].

MCE has not independently confirmed the accuracy of these methods. They are for reference only.
分子量

172.19

Formula

C6H12N4O2
CAS 号

1863117-91-2
运输条件

Room temperature in continental US; may vary elsewhere.
储存方式

Please store the product under the recommended conditions in the Certificate of Analysis.
参考文献

  • [1]. Yang T, et al. Photo-lysine captures proteins that bind lysine post-translational modifications. Nat Chem Biol. 2016 Feb;12(2):70-2.

DiAzKs hydrochloride(Synonyms: H-L-Photo-lysine hydrochloride)

发表于由

DiAzKs hydrochloride;(Synonyms: H-L-Photo-lysine hydrochloride) 纯度: ge;98.0%

DiAzKs (H-L-Photo-lysine) hydrochloride 是一种含有重氮基的赖氨酸氨基酸，也是一种光交联剂。DiAzKs hydrochloride 可以选择性的插入到蛋白质中，并用于在体外和活细胞中交联蛋白质-蛋白质相互作用。DiAzKs hydrochloride 用作紫外光激活的光交联探针。

DiAzKs hydrochlorideamp;;(Synonyms: H-L-Photo-lysine hydrochloride)

DiAzKs hydrochloride Chemical Structure

CAS No. : 2421187-79-1

规格 价格 是否有货 数量
10;mM;*;1 mL in DMSO ￥1050 In-stock
5 mg ￥1650 In-stock
10 mg ￥2600 In-stock
50 mg ￥6500 In-stock
100 mg ; 询价 ;
200 mg ; 询价 ;

* Please select Quantity before adding items.

DiAzKs hydrochloride 相关产品

bull;相关化合物库:

  • Bioactive Compound Library Plus

生物活性

DiAzKs (H-L-Photo-lysine) hydrochloride is a diazirine-containing lysine amino acid and is a photo-cross-linker. DiAzKs hydrochloride can site-selective incorporated into proteins and is used to crosslink protein-protein interactions in vitro and in living cells. DiAzKs hydrochloride acts as a UV light-activated photo-crosslinking probe[1][2][3].

体外研究
(In Vitro)

Photo-lysine, which is readily incorporated into proteins by native mammalian translation machinery, can be used to capture and identify proteins that recognize lysine post-translational modifications (PTMs), including ‘readers’ and ‘erasers’ of histone modifications[2].

MCE has not independently confirmed the accuracy of these methods. They are for reference only.
分子量

308.76

Formula

C11H21ClN4O4
CAS 号

2421187-79-1
运输条件

Room temperature in continental US; may vary elsewhere.
储存方式

4deg;C, sealed storage, away from moisture

*In solvent : -80deg;C, 6 months; -20deg;C, 1 month (sealed storage, away from moisture)
溶解性数据
In Vitro:;

DMSO : 250 mg/mL (809.69 mM; Need ultrasonic)

H2O : 50 mg/mL (161.94 mM; Need ultrasonic)

配制储备液
浓度 溶剂体积 质量 1 mg 5 mg 10 mg
1 mM 3.2388 mL 16.1938 mL 32.3876 mL
5 mM 0.6478 mL 3.2388 mL 6.4775 mL
10 mM 0.3239 mL 1.6194 mL 3.2388 mL

*

请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液；一旦配成溶液，请分装保存，避免反复冻融造成的产品失效。
储备液的保存方式和期限：-80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)。-80°C 储存时，请在 6 个月内使用，-20°C 储存时，请在 1 个月内使用。

In Vivo:

请根据您的实验动物和给药方式选择适当的溶解方案。以下溶解方案都请先按照 In Vitro 方式配制澄清的储备液，再依次添加助溶剂：

——为保证实验结果的可靠性，澄清的储备液可以根据储存条件，适当保存；体内实验的工作液，建议您现用现配，当天使用； 以下溶剂前显示的百
分比是指该溶剂在您配制终溶液中的体积占比；如在配制过程中出现沉淀、析出现象，可以通过加热和/或超声的方式助溶

  • 1.

    请依序添加每种溶剂：;10% DMSO ;; 40% PEG300 ;; 5% Tween-80 ;; 45% saline

    Solubility: ≥ 2.08 mg/mL (6.74 mM); Clear solution

    此方案可获得 ≥ 2.08 mg/mL (6.74 mM，饱和度未知) 的澄清溶液。

    以 1 mL 工作液为例，取 100 μL 20.8 mg/mL 的澄清 DMSO 储备液加到 400 μL PEG300 中，混合均匀；向上述体系中加入50 μL Tween-80，混合均匀；然后继续加入 450 μL生理盐水定容至 1 mL。

    将 0.9 g 氯化钠，完全溶解于 100 mL ddH₂O 中，得到澄清透明的生理盐水溶液

  • 2.

    请依序添加每种溶剂：;10% DMSO ;; 90% (20% SBE-β-CD in saline)

    Solubility: ≥ 2.08 mg/mL (6.74 mM); Clear solution

    此方案可获得 ≥ 2.08 mg/mL (6.74 mM，饱和度未知) 的澄清溶液。

    以 1 mL 工作液为例，取 100 μL 20.8 mg/mL 的澄清 DMSO 储备液加到 900 μL 20% 的 SBE-β-CD 生理盐水水溶液中，混合均匀。

    将 2 g 磺丁基醚 β-环糊精加入 5 mL 生理盐水中，再用生理盐水定容至 10 mL，完全溶解，澄清透明
*以上所有助溶剂都可在 MCE 网站选购。
参考文献

  • [1]. Ai HW, et al. Probing protein-protein interactions with a genetically encoded photo-crosslinking amino acid. Chembiochem. 2011 Aug 16;12(12):1854-7.

    [2]. Chatterjee A, et al. Efficient viral delivery system for unnatural amino acid mutagenesis in mammalian cells. Proc Natl Acad Sci U S A. 2013 Jul 16;110(29):11803-8.

    [3]. Yang T, et al. Photo-lysine captures proteins that bind lysine post-translational modifications. Nat Chem Biol. 2016 Feb;12(2):70-2.

NETZSCH耐驰Photo-DSC 204 F1 Phoenix®光量热系统

发表于由
NETZSCH耐驰Photo-DSC 204 F1 Phoenix®光量热系统

NETZSCH耐驰Photo-DSC 204 F1 Phoenix®光量热系统

产品编号：
市场价：￥0.00元
会员价：￥0.00元
品牌：NETZSCH耐驰
生产厂家：NETZSCH耐驰

  • 产品概览
  • 技术参数
  • 订购信息
  • 相关资料
  • 相关产品

NETZSCH耐驰Photo-DSC 204 F1 Phoenix®光量热系统

光固化体系的主要优点在于快速的反应时间。单体溶液（通常没有溶剂）在低温下可能仅在几秒之内就已完成固化。在双固化粘合剂或涂料领域，经常结合使用热固化与光固化两种方式。

针对这一特定领域，耐驰公司最新推出了 Photo-DSC 204 F1 Phoenix®，能够工作于 -100℃ … 200℃ 的温度范围内。该仪器有着真空密闭的测量单元，吹扫气流量则由内置的质量流量计（MFC）来调节控制。

Photo-DSC 204 F1 能够使用大多数标准的用于阳离子或辐射固化体系的实验室与工业灯管。对于粘合剂的分析，我们推荐 DELO 公司制造 DELOLUX04 。

DSC 204 F1 Phoenix® 经过改造的自动提盖装置保证了两根光导管与样品和参比间的距离固定。这确保了测量结果的高度的重复性。

对于其他高于 200℃ 的常规 DSC 分析，可以对标准的提盖装置进行改造，或者也可使用手动提盖。

对于需要频繁或重复测样的场合，建议配备自动进样器 ASC（一次可装载多至 64 个样品）

 

Photo-DSC204F1 – 技术参数（配备 DELOLUX 04）

  • 温度范围：-100℃ … 200℃
  • 坩埚：敞口 Al 坩埚
  • 灯管型号：DELOLUX 04 ( Hg )
  • 功率：< 9900 mW/cm2
  • 波长范围：315 … 500 nm
  • 照射时间：0.1 … 1000 s
  • 孔径：8, 4, 2 mm
  • 滤光片：取决于应用