Protein C activity is measured using the reagents, both with a partial thromboplastin time (aPTT) system as well as with a chromogenic substrate (pGlu-Pro-Arg-mNA) in a photometric method[1].
MCE has not independently confirmed the accuracy of these methods. They are for reference only.
分子量
532.55
Formula
C23H32N8O7
CAS 号
130835-45-9
Sequence
{pGlu}-Pro-Arg-MNA
Sequence Shortening
{pGlu}-PR-MNA
运输条件
Room temperature in continental US; may vary elsewhere.
储存方式
Please store the product under the recommended conditions in the Certificate of Analysis.
Solvent Solubility
In Vitro:;
H2O
Peptide Solubility and Storage Guidelines:
1.;;Calculate the length of the peptide.
2.;;Calculate the overall charge of the entire peptide according to the following table:
;
Contents
Assign value
Acidic amino acid
Asp (D), Glu (E), and the C-terminal -COOH.
-1
Basic amino acid
Arg (R), Lys (K), His (H), and the N-terminal -NH2
+1
Neutral amino acid
Gly (G), Ala (A), Leu (L), Ile (I), Val (V), Cys (C), Met (M), Thr (T), Ser (S), Phe (F), Tyr (Y), Trp (W), Pro (P), Asn (N), Gln (Q)
0
3.;;Recommended solution:
Overall charge of peptide
Details
Negative (lt;0)
1.;;Try to dissolve the peptide in water first. 2.;;If water fails, add NH4OH (lt;50 μL). 3.;;If the peptide still does not dissolve, add DMSO (50-100 μL) to solubilize the peptide.
Positive (gt;0)
1.;;Try to dissolve the peptide in water first. 2.;;If water fails, try dissolving the peptide in a 10%-30% acetic acid solution. 3.;;If the peptide still does not dissolve, try dissolving the peptide in a small amount of DMSO.
Zero (=0)
1.;;Try to dissolve the peptide in organic solvent (acetonitrile, methanol, etc.) first. 2.;;For very hydrophobic peptides, try dissolving the peptide in a small amount of DMSO, and then dilute the solution with water to the desired concentration.
参考文献
[1]. Girolami A, et al. Heterozygous protein-S deficiency: a study of a large kindred. Acta Haematol. 1990;84(3):162-8.
pGlu-Pro-Arg-MNA monoacetate is a chromogenic substrate.
体外研究 (In Vitro)
Protein C activity is measured using the reagents, both with a partial thromboplastin time (aPTT) system as well as with a chromogenic substrate (pGlu-Pro-Arg-mNA) in a photometric method[1].
MCE has not independently confirmed the accuracy of these methods. They are for reference only.
分子量
592.60
Formula
C25H36N8O9
CAS 号
2070009-26-4
Sequence
{pGlu}-Pro-Arg-MNA
Sequence Shortening
{pGlu}-PR-MNA
运输条件
Room temperature in continental US; may vary elsewhere.