NBD-LLLLpY

发表于2024年10月25日由Whatman官网

NBD-LLLLpY;

NBD-LLLLpY Y 是一种酶促形成的核内肽，用于选择性杀伤人类诱导的多能干细胞。

NBD-LLLLpY Chemical Structure

规格		是否有货
100 mg	;	询价	;
250 mg	;	询价	;
500 mg	;	询价	;

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生物活性	NBD-LLLLpY is an enzymatically forming intranuclear peptide for selectively killing human induced pluripotent stem cells.
分子量	947.97
Formula	C₄₂H₆₂N₉O₁₄P
运输条件	Room temperature in continental US; may vary elsewhere.
储存方式	Please store the product under the recommended conditions in the Certificate of Analysis.
参考文献	[1]. Liu S, et al. Enzymatically Forming Intranuclear Peptide Assemblies for Selectively Killing Human Induced Pluripotent Stem Cells. J Am Chem Soc. 2021 Sep 29;143(38):15852-15862.

IKKγ NBD Inhibitory Peptide

发表于2024年8月1日由Whatman官网

IKKγ NBD Inhibitory Peptide;

IKKγ NBD Inhibitory Peptide是 NEMO 结合域的多肽，对应着 NEMO 氨基端α螺旋区，可以阻断 TNFα 诱导的 NF-kB 激活。

IKKγ NBD Inhibitory Peptide Chemical Structure

CAS No. : 372146-18-4

规格		是否有货
100 mg	;	询价	;
250 mg	;	询价	;
500 mg	;	询价	;

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IKKgamma; NBD Inhibitory Peptide 的其他形式现货产品:

IKKgamma; NBD Inhibitory Peptide TFA

生物活性

IKKγ NBD Inhibitory Peptide is a NEMO-binding domain peptide (NBD peptide) corresponding to the NEMO amino-terminal alpha-helical region, which is shown to block TNF-alpha-induced NF-kB activation^[1].

分子量

3693.30

Formula

C₁₇₀H₂₅₉N₄₉O₄₂S₁

CAS 号

372146-18-4

Sequence Shortening

DRQIKIWFQNRRMKWKKTALDWSWLQTE

运输条件

Room temperature in continental US; may vary elsewhere.

储存方式

Please store the product under the recommended conditions in the Certificate of Analysis.

Solvent Solubility

In Vitro:;

H₂O

Peptide Solubility and Storage Guidelines:

1.;;Calculate the length of the peptide.

2.;;Calculate the overall charge of the entire peptide according to the following table:

;	Contents	Assign value
Acidic amino acid	Asp (D), Glu (E), and the C-terminal -COOH.	-1
Basic amino acid	Arg (R), Lys (K), His (H), and the N-terminal -NH₂	+1
Neutral amino acid	Gly (G), Ala (A), Leu (L), Ile (I), Val (V), Cys (C), Met (M), Thr (T), Ser (S), Phe (F), Tyr (Y), Trp (W), Pro (P), Asn (N), Gln (Q)	0

3.;;Recommended solution:

Overall charge of peptide	Details
Negative (lt;0)	1.;;Try to dissolve the peptide in water first. 2.;;If water fails, add NH₄OH (lt;50 μL). 3.;;If the peptide still does not dissolve, add DMSO (50-100 μL) to solubilize the peptide.
Positive (gt;0)	1.;;Try to dissolve the peptide in water first. 2.;;If water fails, try dissolving the peptide in a 10%-30% acetic acid solution. 3.;;If the peptide still does not dissolve, try dissolving the peptide in a small amount of DMSO.
Zero (=0)	1.;;Try to dissolve the peptide in organic solvent (acetonitrile, methanol, etc.) first. 2.;;For very hydrophobic peptides, try dissolving the peptide in a small amount of DMSO, and then dilute the solution with water to the desired concentration.

参考文献

[1]. Zhao J, et al. Development of novel NEMO-binding domain mimetics for inhibiting IKK/NF-κB activation. PLoS Biol. 2018 Jun 11;16(6):e2004663.

NBD-Cl(Synonyms: NBD chloride)

发表于2024年4月23日由Whatman官网

NBD-Cl;(Synonyms: NBD chloride) 纯度: 99.14%

NBD-Cl 是一种非荧光剂，与巯基或氨基反应后高度荧光。

NBD-Cl Chemical Structure

CAS No. : 10199-89-0

规格	价格	是否有货	数量
Free Sample (0.1-0.5 mg)	;	Apply now	;
10;mM;*;1 mL in DMSO	￥660	In-stock
500 mg	￥600	In-stock
1 g	￥1000	In-stock
5 g	;	询价	;
10 g	;	询价	;

* Please select Quantity before adding items.

NBD-Cl 相关产品

bull;相关化合物库:

Bioactive Compound Library Plus

生物活性

NBD-Cl is a nonfluorescent reagent which becomes highly fluorescent after reaction with thiol or amino groups^[1].

体外研究
(In Vitro)

NBD-Cl (NBD chloride) forms highly fluorescent derivatives for detection of all the protein amino acids. In addition, NBD-Cl provides a simple and sensitive method for determination of N-terminal amino acids. The differences in intensity and color of fluorescence can be used to advantage to identify prolyl peptides^[1].

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

分子量

199.55

Formula

C₆H₂ClN₃O₃

CAS 号

10199-89-0

运输条件

Room temperature in continental US; may vary elsewhere.

储存方式

4deg;C, protect from light

*In solvent : -80deg;C, 6 months; -20deg;C, 1 month (protect from light)

溶解性数据

In Vitro:;

DMSO : ≥ 100 mg/mL (501.13 mM)

H₂O : 0.67 mg/mL (3.36 mM; Need ultrasonic)

* “≥” means soluble, but saturation unknown.

配制储备液

浓度溶剂体积质量	1 mg	5 mg	10 mg

1 mM	5.0113 mL	25.0564 mL	50.1128 mL
5 mM	1.0023 mL	5.0113 mL	10.0226 mL
10 mM	0.5011 mL	2.5056 mL	5.0113 mL

请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液；一旦配成溶液，请分装保存，避免反复冻融造成的产品失效。
储备液的保存方式和期限：-80°C, 6 months; -20°C, 1 month (protect from light)。-80°C 储存时，请在 6 个月内使用，-20°C 储存时，请在 1 个月内使用。

In Vivo:

请根据您的实验动物和给药方式选择适当的溶解方案。以下溶解方案都请先按照 In Vitro 方式配制澄清的储备液，再依次添加助溶剂：

——为保证实验结果的可靠性，澄清的储备液可以根据储存条件，适当保存；体内实验的工作液，建议您现用现配，当天使用；以下溶剂前显示的百
分比是指该溶剂在您配制终溶液中的体积占比；如在配制过程中出现沉淀、析出现象，可以通过加热和/或超声的方式助溶

1.

请依序添加每种溶剂：;10% DMSO ;; 90% (20% SBE-β-CD in saline)

Solubility: ≥ 2.5 mg/mL (12.53 mM); Clear solution

此方案可获得 ≥ 2.5 mg/mL (12.53 mM，饱和度未知) 的澄清溶液。

以 1 mL 工作液为例，取 100 μL 25.0 mg/mL 的澄清 DMSO 储备液加到 900 μL 20% 的 SBE-β-CD 生理盐水水溶液中，混合均匀。

将 2 g 磺丁基醚 β-环糊精加入 5 mL 生理盐水中，再用生理盐水定容至 10 mL，完全溶解，澄清透明

*以上所有助溶剂都可在 MCE 网站选购。

参考文献

[1]. Fager RS, et al. The use of NBD chloride (7 chloro-4-nitrobenzo-2-oxa-1,3-diazole) in detecting amino acids and as an N-terminal reagent. Anal Biochem. 1973 May;53(1):290-4.

Kinase Assay ^[1]	Fluorescence Measurements^[1] Fluorescence spectra are recorded on a Hitachi MPF-24 Fluorescence Spectrophotometer. The emission spectrum is determined at the optimal wavelength of 464 nm. Thin-layer chromatography is carried out on Brinkmann glass supported analytical silica G thin-layer plates without fluorescent indicator. The NBD derivatives are visualized on thin-layer plates either by examination under an ultraviolet lamp with a long wavelength filter or by using the same lamp with a 2×2-in. Corning 5-57 filter and viewing the spots through a 2×2-in. Corning 3-68 filter. The former method produces a more intense fluorescence, but the latter produces a much greater contrast appearing as white spots against a dark background. NBD-Cl (NBD chloride) is allowed to react with amino acids and peptides under the conditions. Into 3 mL Pyrex test tubes are pipette the following: (1) 2.0 mL of ethanol, (2) 0.1 mL NBD-Cl solution in ethanol 1.41 pg/mL, (3) 0.1 mL ethanol saturated with sodium acetate, (4) 0.1 mL of amino acid solution 400-600μg/mL, and the mixture incubated at 75°C for 20 min. For reaction with peptides, 1.5 mL of ethanol, 0.1 mL ethanolic sodium acetate, 0.4 mL ethanolic NBD-Cl, 1.41 μg/mL, are added to 0.4 ml of the peptide and incubated under the same conditions. MCE has not independently confirmed the accuracy of these methods. They are for reference only.
参考文献	[1]. Fager RS, et al. The use of NBD chloride (7 chloro-4-nitrobenzo-2-oxa-1,3-diazole) in detecting amino acids and as an N-terminal reagent. Anal Biochem. 1973 May;53(1):290-4.

Kinase Assay
^[1]

Fluorescence Measurements^[1]
Fluorescence spectra are recorded on a Hitachi MPF-24 Fluorescence Spectrophotometer. The emission spectrum is determined at the optimal wavelength of 464 nm. Thin-layer chromatography is carried out on Brinkmann glass supported analytical silica G thin-layer plates without fluorescent indicator. The NBD derivatives are visualized on thin-layer plates either by examination under an ultraviolet lamp with a long wavelength filter or by using the same lamp with a 2×2-in. Corning 5-57 filter and viewing the spots through a 2×2-in. Corning 3-68 filter. The former method produces a more intense fluorescence, but the latter produces a much greater contrast appearing as white spots against a dark background. NBD-Cl (NBD chloride) is allowed to react with amino acids and peptides under the conditions. Into 3 mL Pyrex test tubes are pipette the following: (1) 2.0 mL of ethanol, (2) 0.1 mL NBD-Cl solution in ethanol 1.41 pg/mL, (3) 0.1 mL ethanol saturated with sodium acetate, (4) 0.1 mL of amino acid solution 400-600μg/mL, and the mixture incubated at 75°C for 20 min. For reaction with peptides, 1.5 mL of ethanol, 0.1 mL ethanolic sodium acetate, 0.4 mL ethanolic NBD-Cl, 1.41 μg/mL, are added to 0.4 ml of the peptide and incubated under the same conditions.

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

参考文献

[1]. Fager RS, et al. The use of NBD chloride (7 chloro-4-nitrobenzo-2-oxa-1,3-diazole) in detecting amino acids and as an N-terminal reagent. Anal Biochem. 1973 May;53(1):290-4.

NBD-F(Synonyms: 4-Fluoro-7-nitrobenzofurazan)

发表于2024年4月18日由Whatman官网

NBD-F;(Synonyms: 4-Fluoro-7-nitrobenzofurazan) 纯度: ge;98.0%

NBD-F (4-Fluoro-7-nitrobenzofurazan) 是一种用于氨基酸分析 (amino acid analysis) 的前荧光试剂。NBD-F 与一级或二级胺反应生成荧光产物，用于分析氨基酸和低分子量胺。

NBD-Famp;;(Synonyms: 4-Fluoro-7-nitrobenzofurazan)

NBD-F Chemical Structure

CAS No. : 29270-56-2

规格	价格	是否有货	数量
10;mM;*;1 mL in DMSO	￥1540	In-stock
50 mg	￥1800	In-stock
100 mg	￥2600	In-stock
200 mg	;	询价	;
500 mg	;	询价	;

* Please select Quantity before adding items.

NBD-F 相关产品

bull;相关化合物库:

Bioactive Compound Library Plus

生物活性

NBD-F (4-Fluoro-7-nitrobenzofurazan) is a pro-fluorescent reagent which is developed for amino acid analysis. NBD-F reacts with primary or secondary amines to produce a fluorescent product and used for analysis of amino acids and low molecular weight amines^[1].

体外研究
(In Vitro)

As the percentage of the organic phase changes, the retention time of NBD-F remains relatively stable, while the retention times of the derivatization products changes. The pH of the mobile phase affects the separation of the NBD-F and the derivatization products^[1]. NBD-F is a fluorescent derivatization reagent that is originally developed for amino acid analysis, and recently applied to the analysis of other amino acid derivatives such as N-methyl-D-aspartic acid and glutathione. The use of NBD-F appears to have several advantages in that the derivatization procedure is simple and its derivatives are highly stable^[2].

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

分子量

183.10

Formula

C₆H₂FN₃O₃

CAS 号

29270-56-2

中文名称

4-氟-7-硝基-2,1,3-苯并氧杂噁二唑

运输条件

Room temperature in continental US; may vary elsewhere.

储存方式

4deg;C, protect from light

*In solvent : -80deg;C, 6 months; -20deg;C, 1 month (protect from light)

溶解性数据

In Vitro:;

DMSO : 100 mg/mL (546.15 mM; Need ultrasonic)

配制储备液

浓度溶剂体积质量	1 mg	5 mg	10 mg

1 mM	5.4615 mL	27.3075 mL	54.6150 mL
5 mM	1.0923 mL	5.4615 mL	10.9230 mL
10 mM	0.5461 mL	2.7307 mL	5.4615 mL

In Vivo:

请根据您的实验动物和给药方式选择适当的溶解方案。以下溶解方案都请先按照 In Vitro 方式配制澄清的储备液，再依次添加助溶剂：

1.

请依序添加每种溶剂：;10% DMSO ;; 40% PEG300 ;; 5% Tween-80 ;; 45% saline

Solubility: ≥ 2.5 mg/mL (13.65 mM); Clear solution

此方案可获得 ≥ 2.5 mg/mL (13.65 mM，饱和度未知) 的澄清溶液。

以 1 mL 工作液为例，取 100 μL 25.0 mg/mL 的澄清 DMSO 储备液加到 400 μL PEG300 中，混合均匀；向上述体系中加入50 μL Tween-80，混合均匀；然后继续加入 450 μL生理盐水定容至 1 mL。

将 0.9 g 氯化钠，完全溶解于 100 mL ddH₂O 中，得到澄清透明的生理盐水溶液

*以上所有助溶剂都可在 MCE 网站选购。

参考文献

[1]. Wu X, et al. Determination of amino acid neurotransmitters in rat hippocampi by HPLC-UV using NBD-F as a derivative. Biomed Chromatogr. 2014 Apr;28(4):459-62.

[2]. Ishikawa T, et al. Development of an LC-MS/MS method for the analysis of free sphingoid bases using 4-fluoro-7-nitrobenzofurazan (NBD-F). Lipids. 2014 Mar;49(3):295-304.

Kinase Assay ^[1]	An accurately weighed quantity (0.0183 g) of NBD-F is transferred into a 1 mL centrifuge tube, dissolved in acetonitrile and made up to volume with the same solvent to produce stock solutions of 0.1 M. The solution is protected from light and stored at -20°C until analyzed. A 100 μL aliquot of mixed amino acids solution or sample supernatant, 175 μL of borate buffer solution, 200 μL of acetonitrile and 25 μL of NBD-F working solution are mixed in a 1.5 mL centrifuge tube. The well-mixed solution is allowed to react at 60°C in the water bath for 7 min, excluding light. NBD-F reacts with amino group and enables amino acids to be detected with UV detection. After cooling to room temperature, 10 μL of the solution is injected into the equilibrated HPLC system^[1]. MCE has not independently confirmed the accuracy of these methods. They are for reference only.
参考文献	[1]. Wu X, et al. Determination of amino acid neurotransmitters in rat hippocampi by HPLC-UV using NBD-F as a derivative. Biomed Chromatogr. 2014 Apr;28(4):459-62. [2]. Ishikawa T, et al. Development of an LC-MS/MS method for the analysis of free sphingoid bases using 4-fluoro-7-nitrobenzofurazan (NBD-F). Lipids. 2014 Mar;49(3):295-304.

Kinase Assay
^[1]

An accurately weighed quantity (0.0183 g) of NBD-F is transferred into a 1 mL centrifuge tube, dissolved in acetonitrile and made up to volume with the same solvent to produce stock solutions of 0.1 M. The solution is protected from light and stored at -20°C until analyzed. A 100 μL aliquot of mixed amino acids solution or sample supernatant, 175 μL of borate buffer solution, 200 μL of acetonitrile and 25 μL of NBD-F working solution are mixed in a 1.5 mL centrifuge tube. The well-mixed solution is allowed to react at 60°C in the water bath for 7 min, excluding light. NBD-F reacts with amino group and enables amino acids to be detected with UV detection. After cooling to room temperature, 10 μL of the solution is injected into the equilibrated HPLC system^[1].

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

参考文献

[1]. Wu X, et al. Determination of amino acid neurotransmitters in rat hippocampi by HPLC-UV using NBD-F as a derivative. Biomed Chromatogr. 2014 Apr;28(4):459-62.

[2]. Ishikawa T, et al. Development of an LC-MS/MS method for the analysis of free sphingoid bases using 4-fluoro-7-nitrobenzofurazan (NBD-F). Lipids. 2014 Mar;49(3):295-304.

NBD-X, SE

发表于2024年4月8日由Whatman官网

NBD-X, SE;

NBD-X、SE 是一种受体荧光团，可用于创建环境敏感的生物偶联物。

NBD-X, SE Chemical Structure

CAS No. : 145195-58-0

规格		是否有货
100 mg	;	询价	;
250 mg	;	询价	;
500 mg	;	询价	;

* Please select Quantity before adding items.

生物活性	NBD-X, SE, the acceptor fluorophore, can be used to create environment sensitive bioconjugates^[1].
分子量	391.34
Formula	C₁₆H₁₇N₅O₇
CAS 号	145195-58-0
运输条件	Room temperature in continental US; may vary elsewhere.
储存方式	Please store the product under the recommended conditions in the Certificate of Analysis.
参考文献	[1]. A Karlström, et al. Dual labeling of a binding protein allows for specific fluorescence detection of native protein. Anal Biochem. 2001 Aug 1;295(1):22-30.

NBD-amine 7-氨基-4-硝基-2,1,3-苯并恶苯并恶二唑品牌：Chemodex

发表于2024年2月23日由Whatman官网

NBD-amine

7-氨基-4-硝基-2,1,3-苯并恶苯并恶二唑

品牌：Chemodex
CAS No.：10199-91-4
储存条件：-20°C
纯度：>99% (HPLC)

产品编号 (生产商编号)	等级	规格	运输包装	零售价(RMB)	库存情况	参考值
CDX-A0176-M100	–	100 mg	–	1,880.00	–	–

* 干冰运输、大包装及大批量的产品需酌情添加运输费用

* 零售价、促销产品折扣、运输费用、库存情况、产品及包装规格可能因各种原因有所变动，恕不另行通知，确切详情请联系上海金畔生物科技有限公司。

NBD-amine 7-氨基-4-硝基-2,1,3-苯并恶苯并恶二唑品牌：Chemodex

发表于2024年2月22日由Whatman官网

NBD-amine

7-氨基-4-硝基-2,1,3-苯并恶苯并恶二唑

品牌：Chemodex
CAS No.：10199-91-4
储存条件：-20°C
纯度：>99% (HPLC)

产品编号 (生产商编号)	等级	规格	运输包装	零售价(RMB)	库存情况	参考值
CDX-A0176-M500	–	500 mg	–	7,550.00	–	–

* 干冰运输、大包装及大批量的产品需酌情添加运输费用

NBD-azide 4-叠氮基-4-硝基-2,1,3-苯并恶苯并恶二唑品牌：Chemodex

发表于2024年2月21日由Whatman官网

NBD-azide

4-叠氮基-4-硝基-2,1,3-苯并恶苯并恶二唑

品牌：Chemodex
CAS No.：10199-90-3
储存条件：-20°C
纯度：>99% (HPLC)

产品编号 (生产商编号)	等级	规格	运输包装	零售价(RMB)	库存情况	参考值
CDX-A0177-M100	–	100 mg	–	1,150.00	–	–

* 干冰运输、大包装及大批量的产品需酌情添加运输费用

NBD-azide 4-叠氮基-4-硝基-2,1,3-苯并恶苯并恶二唑品牌：Chemodex

发表于2024年2月21日由Whatman官网

NBD-azide

4-叠氮基-4-硝基-2,1,3-苯并恶苯并恶二唑

品牌：Chemodex
CAS No.：10199-90-3
储存条件：-20°C
纯度：>99% (HPLC)

产品编号 (生产商编号)	等级	规格	运输包装	零售价(RMB)	库存情况	参考值
CDX-A0177-M500	–	500 mg	–	4,440.00	–	–

* 干冰运输、大包装及大批量的产品需酌情添加运输费用

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标签归档：nbd

NBD-LLLLpY

IKKγ NBD Inhibitory Peptide

NBD-Cl(Synonyms: NBD chloride)

NBD-Cl 相关产品

bull;相关化合物库:

NBD-F(Synonyms: 4-Fluoro-7-nitrobenzofurazan)

NBD-F 相关产品

bull;相关化合物库:

NBD-X, SE

NBD-amine 7-氨基-4-硝基-2,1,3-苯并恶苯并恶二唑品牌：Chemodex

NBD-amine

7-氨基-4-硝基-2,1,3-苯并恶苯并恶二唑

NBD-amine 7-氨基-4-硝基-2,1,3-苯并恶苯并恶二唑品牌：Chemodex

NBD-amine

7-氨基-4-硝基-2,1,3-苯并恶苯并恶二唑

NBD-azide 4-叠氮基-4-硝基-2,1,3-苯并恶苯并恶二唑品牌：Chemodex

NBD-azide

4-叠氮基-4-硝基-2,1,3-苯并恶苯并恶二唑

NBD-azide 4-叠氮基-4-硝基-2,1,3-苯并恶苯并恶二唑品牌：Chemodex

NBD-azide

4-叠氮基-4-硝基-2,1,3-苯并恶苯并恶二唑

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