Myelin Basic Protein(87-99) TFA Chemical Structure
规格
是否有货
100 mg
;
询价
;
250 mg
;
询价
;
500 mg
;
询价
;
* Please select Quantity before adding items.
生物活性
Myelin Basic Protein(87-99) TFA is an encephalitogenic peptide that induces basic protein-specific T cell proliferation. Myelin Basic Protein(87-99) TFA causes a Th1 polarization in peripheral blood mononuclear cells with is implicated of multiple sclerosis (MS)[1][2].
Room temperature in continental US; may vary elsewhere.
储存方式
Please store the product under the recommended conditions in the Certificate of Analysis.
参考文献
[1]. R E Jones, et al. The synthetic 87-99 peptide of myelin basic protein is encephalitogenic in Buffalo rats. J Neuroimmunol. 1992 Apr;37(3):203-12.
[2]. George Deraos, et al. Citrullination of linear and cyclic altered peptide ligands from myelin basic protein (MBP(87-99)) epitope elicits a Th1 polarized response by T cells isolated from multiple sclerosis patients: implications in triggering disease. J Med Chem. 2008 Dec 25;51(24):7834-42.
Myelin Oligodendrocyte Glycoprotein Peptide (35-55), mouse, rat 是中枢神经鞘的一个次要成分。Myelin Oligodendrocyte Glycoprotein Peptide (35-55), mouse, rat引发复发缓解性神经系统疾病，伴有广泛的斑块样脱髓鞘。还能诱导强效的 T细胞和 B 细胞反应，同时是高度脑源性的。
Myelin Oligodendrocyte Glycoprotein Peptide (35-55), mouse, rat Chemical Structure
CAS No. : 149635-73-4
规格
价格
是否有货
1 mg
￥1500
询问价格 货期
5 mg
￥4500
询问价格 货期
10 mg
￥7600
询问价格 货期
* Please select Quantity before adding items.
Myelin Oligodendrocyte Glycoprotein Peptide (35-55), mouse, rat 的其他形式现货产品:
Myelin Oligodendrocyte Glycoprotein Peptide (35-55), mouse, rat TFA
生物活性
Myelin Oligodendrocyte Glycoprotein Peptide (35-55), mouse, rat is a minor component of CNS myelin. Myelin Oligodendrocyte Glycoprotein Peptide (35-55), mouse, rat produces a relapsing-remitting neurological disease with extensive plaque-like demyelination. Myelin Oligodendrocyte Glycoprotein Peptide (35-55), mouse, rat induces strong T and B cell responses and is highly encephalitogenic[1][2][3].
体内研究 (In Vivo)
Myelin Oligodendrocyte Glycoprotein Peptide (35-55), mouse, rat (MOG) is a highly autoantigenic molecule capable of inducing severe demyelinating disease[2].
MCE has not independently confirmed the accuracy of these methods. They are for reference only.
Mice receiving 50 μg and 100 μg of peptide has more dramatic weight losses during the course of the disease than mice injected with 150 μg.
分子量
2581.95
Formula
C118H177N35O29S
CAS 号
149635-73-4
Sequence Shortening
MEVGWYRSPFSRVVHLYRNGK
运输条件
Room temperature in continental US; may vary elsewhere.
储存方式
Please store the product under the recommended conditions in the Certificate of Analysis.
Solvent Solubility
In Vitro:;
H2O
Peptide Solubility and Storage Guidelines:
1.;;Calculate the length of the peptide.
2.;;Calculate the overall charge of the entire peptide according to the following table:
;
Contents
Assign value
Acidic amino acid
Asp (D), Glu (E), and the C-terminal -COOH.
-1
Basic amino acid
Arg (R), Lys (K), His (H), and the N-terminal -NH2
+1
Neutral amino acid
Gly (G), Ala (A), Leu (L), Ile (I), Val (V), Cys (C), Met (M), Thr (T), Ser (S), Phe (F), Tyr (Y), Trp (W), Pro (P), Asn (N), Gln (Q)
0
3.;;Recommended solution:
Overall charge of peptide
Details
Negative (lt;0)
1.;;Try to dissolve the peptide in water first. 2.;;If water fails, add NH4OH (lt;50 μL). 3.;;If the peptide still does not dissolve, add DMSO (50-100 μL) to solubilize the peptide.
Positive (gt;0)
1.;;Try to dissolve the peptide in water first. 2.;;If water fails, try dissolving the peptide in a 10%-30% acetic acid solution. 3.;;If the peptide still does not dissolve, try dissolving the peptide in a small amount of DMSO.
Zero (=0)
1.;;Try to dissolve the peptide in organic solvent (acetonitrile, methanol, etc.) first. 2.;;For very hydrophobic peptides, try dissolving the peptide in a small amount of DMSO, and then dilute the solution with water to the desired concentration.
参考文献
[1]. Ichikawa M, et al. Analysis of the fine B cell specificity during the chronic/relapsing course of a multiple sclerosis-like disease in Lewis rats injected with the encephalitogenic myelin oligodendrocyte glycoprotein peptide 35-55. J Immunol. 1996 Jul 15;157(2):919-26.
[2]. Slavin A, et al. Induction of a multiple sclerosis-like disease in mice with an immunodominant epitope of myelin oligodendrocyte glycoprotein. Autoimmunity. 1998;28(2):109-20.
[3]. Zhang GX, et al. T cell and antibody responses in remitting-relapsing experimental autoimmune encephalomyelitis in (C57BL/6 x SJL) F1 mice. J Neuroimmunol. 2004 Mar;148(1-2):1-10.
Myelin Basic Protein (MHP4-14) 是一种合成肽，包含髓鞘碱性蛋白的残基4-14，是一种非常有选择性的PKC底物 (Km=7 μM)。Myelin Basic Protein 不被环腺苷酸依赖性蛋白激酶、酪蛋白激酶 I 和 II、Ca2+/钙调素依赖性蛋白激酶II或磷酸化酶激酶磷酸化，可常规用于测定粗组织提取物中低背景的蛋白激酶C。
Myelin Basic Protein Chemical Structure
CAS No. : 126768-94-3
规格
是否有货
100 mg
;
询价
;
250 mg
;
询价
;
500 mg
;
询价
;
* Please select Quantity before adding items.
Myelin Basic Protein 的其他形式现货产品:
Myelin Basic Protein TFA
生物活性
Myelin Basic Protein (MHP4-14), a synthetic peptide comprising residues 4-14 of myelin basic protein, is a very selective PKC substrate (Km=7 μM). Myelin Basic Protein is not phosphorylated by cyclic AMP-dependent protein kinase, casein kinases I and II, Ca2+/calmodulin-dependent protein kinase II, or phosphorylase kinase, and can be routinely used for the assay of protein kinase C with low background in the crude tissue extracts[1][2].
体外研究 (In Vitro)
Once MBP4-14 is phosphorylated, it is not dephosphorylated by okadaic acid-sensitive phosphatases (protein phosphatases 1, 2A and 3) or other protein phosphatases such as calcineurin and/or PP 2C present in hippocampal homogenates. Therefore, MBP4-14 can be used for PKC assay in crude extracts of neural tissue[2].
MCE has not independently confirmed the accuracy of these methods. They are for reference only.
分子量
1390.59
Formula
C60H103N21O17
CAS 号
126768-94-3
Sequence
Gln-Lys-Arg-Pro-Ser-Gln-Arg-Ser-Lys-Tyr-Leu
Sequence Shortening
QKRPSQRSKYL
运输条件
Room temperature in continental US; may vary elsewhere.
储存方式
Please store the product under the recommended conditions in the Certificate of Analysis.
Solvent Solubility
In Vitro:;
H2O
Peptide Solubility and Storage Guidelines:
1.;;Calculate the length of the peptide.
2.;;Calculate the overall charge of the entire peptide according to the following table:
;
Contents
Assign value
Acidic amino acid
Asp (D), Glu (E), and the C-terminal -COOH.
-1
Basic amino acid
Arg (R), Lys (K), His (H), and the N-terminal -NH2
+1
Neutral amino acid
Gly (G), Ala (A), Leu (L), Ile (I), Val (V), Cys (C), Met (M), Thr (T), Ser (S), Phe (F), Tyr (Y), Trp (W), Pro (P), Asn (N), Gln (Q)
0
3.;;Recommended solution:
Overall charge of peptide
Details
Negative (lt;0)
1.;;Try to dissolve the peptide in water first. 2.;;If water fails, add NH4OH (lt;50 μL). 3.;;If the peptide still does not dissolve, add DMSO (50-100 μL) to solubilize the peptide.
Positive (gt;0)
1.;;Try to dissolve the peptide in water first. 2.;;If water fails, try dissolving the peptide in a 10%-30% acetic acid solution. 3.;;If the peptide still does not dissolve, try dissolving the peptide in a small amount of DMSO.
Zero (=0)
1.;;Try to dissolve the peptide in organic solvent (acetonitrile, methanol, etc.) first. 2.;;For very hydrophobic peptides, try dissolving the peptide in a small amount of DMSO, and then dilute the solution with water to the desired concentration.
参考文献
[1]. Farrar YJ, et al. A phosphatase resistant substrate for the assay of protein kinase C in crude tissue extracts. Biochem Biophys Res Commun. 1991;180(2):694-701.
[2]. Yasuda I, et al. A synthetic peptide substrate for selective assay of protein kinase C. Biochem Biophys Res Commun. 1990;166(3):1220-1227.
[1]. Lei Bao, et al. The critical role of IL-12p40 in initiating, enhancing, and perpetuating pathogenic events in murine experimental autoimmune neuritis. Brain Pathol. 2002 Oct;12(4):420-9.
[2]. L P Zou, et al. P0 protein peptide 180-199 together with pertussis toxin induces experimental autoimmune neuritis in resistant C57BL/6 mice. J Neurosci Res. 2000 Dec 1;62(5):717-21.
[1]. Lei Bao, et al. The critical role of IL-12p40 in initiating, enhancing, and perpetuating pathogenic events in murine experimental autoimmune neuritis. Brain Pathol. 2002 Oct;12(4):420-9.
[2]. L P Zou, et al. P0 protein peptide 180-199 together with pertussis toxin induces experimental autoimmune neuritis in resistant C57BL/6 mice. J Neurosci Res. 2000 Dec 1;62(5):717-21.
