4-Methylumbelliferyl phosphate (4-MUP) is a fluorogenic substrate of alkaline phosphatases^[1].

4-Methylumbelliferyl phosphate monoester previously described as one of a series of 4-methylumbelliferone conjugates is later found to be the PlP2-pyrophosphate diester and is not hydrolysed by serum phosphomonoesterases . 4-Methylumbelliferyl phosphate has a significant advantage over currently available substrates in that its useful concentration range extends to about 0.1 μM^[1].
If the concentration of 4-Methylumbelliferyl phosphate is held constant and the pH is varied, the activity of the enzyme passes through a maximum^[1].

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

256.15

C₁₀H₉O₆P

3368-04-5

4-甲基伞形酮磷酸酯

Room temperature in continental US; may vary elsewhere.

-20deg;C, protect from light

*In solvent : -80deg;C, 6 months; -20deg;C, 1 month (protect from light)

In Vitro:;

H₂O : 20.83 mg/mL (81.32 mM; Need ultrasonic)

*

请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液；一旦配成溶液，请分装保存，避免反复冻融造成的产品失效。
储备液的保存方式和期限：-80°C, 6 months; -20°C, 1 month (protect from light)。-80°C 储存时，请在 6 个月内使用，-20°C 储存时，请在 1 个月内使用。

• [1]. Fernley HN, et al. Kinetic behaviour of calf-intestinal alkaline phosphatase with 4-methylumbelliferyl phosphate. Biochem J. 1965 Oct;97(1):95-103.