4-Methylumbelliferyl-β-D-glucuronide hydrate is a fluorogenic substrat (λ_ex=362 nm , λ_em=445 nm).

4-Methylumbelliferyl-β-D-glucuronide hydrate is a fluorogenic substrat with λ_ex=362 nm and λ_em=445 nm. The half-saturation constants, K_m, that are calculated for sewage samples are close to the K_m values calculated based on experimental plots presented previously for freshwaters^[1].

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

370.31

C₁₆H₁₈O₁₀

881005-91-0

Room temperature in continental US; may vary elsewhere.

4deg;C, protect from light

*In solvent : -80deg;C, 6 months; -20deg;C, 1 month (protect from light)

DMSO : 150 mg/mL (405.07 mM; Need ultrasonic)

H₂O : 5.2 mg/mL (14.04 mM; Need ultrasonic and warming)

请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液；一旦配成溶液，请分装保存，避免反复冻融造成的产品失效。
储备液的保存方式和期限：-80°C, 6 months; -20°C, 1 month (protect from light)。-80°C 储存时，请在 6 个月内使用，-20°C 储存时，请在 1 个月内使用。

• [1]. George I, et al. Use of beta-D-galactosidase and beta-D-glucuronidase activities for quantitative detection of totaland fecal coliforms in wastewater. Can J Microbiol. 2001 Jul;47(7):670-5.

GLUase activity of E. coli cells is measured. Three millilitres of 4-Methylumbelliferyl-β-D-glucuronide hydrate (MUGlu) solution (55 mg of hydrated MUGlu and 20 mL of Triton X-100 in 50 mL of sterile water) is added to each flask (final concentration:165 mg/L). The incubation temperature is 44°C. One hundred microlitres of 2 M NaOH solution is added to each 2.9-mL aliquot to obtain a pH>10 before the fluorescence measurement^[1].

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

• [1]. George I, et al. Use of beta-D-galactosidase and beta-D-glucuronidase activities for quantitative detection of totaland fecal coliforms in wastewater. Can J Microbiol. 2001 Jul;47(7):670-5.