标签归档：met

[Des-Tyr1]-Met-Enkephalin

发表于由

[Des-Tyr1]-Met-Enkephalin;

[Des-Tyr1]-Met-Enkephalin 是一种四肽，是脑啡肽的降解产物。

[Des-Tyr1]-Met-Enkephalinamp;;

[Des-Tyr1]-Met-Enkephalin Chemical Structure

CAS No. : 61370-88-5

规格 是否有货
100 mg ; 询价 ;
250 mg ; 询价 ;
500 mg ; 询价 ;

* Please select Quantity before adding items.

生物活性

[Des-Tyr1]-Met-Enkephalin, a tetrapeptide, is a degradationproduct of enkephalins[1].

体外研究
(In Vitro)

Tetrapeptide H-gily-gly-phe-met-oh shows very littleactivity using the mouse[1].

MCE has not independently confirmed the accuracy of these methods. They are for reference only.
分子量

410.49

Formula

C18H26N4O5S
CAS 号

61370-88-5
Sequence Shortening

GGFM
运输条件

Room temperature in continental US; may vary elsewhere.
储存方式

Please store the product under the recommended conditions in the Certificate of Analysis.
参考文献

  • [1]. J M Hambrook, et al. Mode of deactivation of the enkephalins by rat and human plasma and rat brain homogenates. Nature. 1976 Aug 26;262(5571):782-3.

N-Formyl-Met-Leu-Phe-Lys(Synonyms: fMLFK)

发表于由

N-Formyl-Met-Leu-Phe-Lys;(Synonyms: fMLFK)

N-Formyl-Met-Leu-Phe-Lys 是一种多肽，为有效、选择性的 FPR1 激动剂，对 FPR1，FPR2 和 FPR2-D2817.32G 的 EC50 值分别为 3.5 nM，6.7 μM 和 0.88 μM。

N-Formyl-Met-Leu-Phe-Lysamp;;(Synonyms: fMLFK)

N-Formyl-Met-Leu-Phe-Lys Chemical Structure

CAS No. : 67247-11-4

规格 是否有货
100 mg ; 询价 ;
250 mg ; 询价 ;
500 mg ; 询价 ;

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生物活性

N-Formyl-Met-Leu-Phe-Lys (fMLFK) is a peptide, acts as a potent and selective agonist of FPR1, with EC50s of 3.5 nM, 6.7 μM and 0.88 μM for FPR1, FPR2 and FPR2-D2817.32G, respectively[1].

IC50 Target

EC50: 3.5 nM (FPR1), 6.7 μM (FPR2), 0.88 μM (FPR2-D2817.32G)[1]
分子量

565.73

Formula

C27H43N5O6S
CAS 号

67247-11-4
Sequence

Formyl-Met-Leu-Phe-Lys
Sequence Shortening

Formyl-MLFK
运输条件

Room temperature in continental US; may vary elsewhere.
储存方式

Please store the product under the recommended conditions in the Certificate of Analysis.
Solvent Solubility
In Vitro:;

H2O

Peptide Solubility and Storage Guidelines:

1.;;Calculate the length of the peptide.

2.;;Calculate the overall charge of the entire peptide according to the following table:

; Contents Assign value
Acidic amino acid Asp (D), Glu (E), and the C-terminal -COOH. -1
Basic amino acid Arg (R), Lys (K), His (H), and the N-terminal -NH2 +1
Neutral amino acid Gly (G), Ala (A), Leu (L), Ile (I), Val (V), Cys (C), Met (M), Thr (T), Ser (S), Phe (F), Tyr (Y), Trp (W), Pro (P), Asn (N), Gln (Q) 0

3.;;Recommended solution:

Overall charge of peptide Details
Negative (lt;0) 1.;;Try to dissolve the peptide in water first.
2.;;If water fails, add NH4OH (lt;50 μL).
3.;;If the peptide still does not dissolve, add DMSO (50-100 μL) to solubilize the peptide.
Positive (gt;0) 1.;;Try to dissolve the peptide in water first.
2.;;If water fails, try dissolving the peptide in a 10%-30% acetic acid solution.
3.;;If the peptide still does not dissolve, try dissolving the peptide in a small amount of DMSO.
Zero (=0) 1.;;Try to dissolve the peptide in organic solvent (acetonitrile, methanol, etc.) first.
2.;;For very hydrophobic peptides, try dissolving the peptide in a small amount of DMSO, and then dilute the solution with water to the desired concentration.
参考文献

  • [1]. He HQ, et al. Structural determinants for the interaction of formyl peptide receptor 2 with peptide ligands. J Biol Chem. 2014 Jan 24;289(4):2295-306.

Met e 1 (shrimp) (rec.) 原肌球蛋白1（虾）（rec.） 品牌：AbFrontier

发表于由

Met e 1 (shrimp) (rec.)

原肌球蛋白1（虾）（rec.）

品牌：AbFrontier
CAS No.：
储存条件：-80℃
纯度：–
产品编号

(生产商编号)

 等级 规格 运输包装 零售价(RMB) 库存情况 参考值

YIF-LF-P0501

 – 0.1 mg –

Met e 1 (shrimp) (rec.) 原肌球蛋白1（虾）（rec.） 品牌：AbFrontier

 – –


* 干冰运输、大包装及大批量的产品需酌情添加运输费用


* 零售价、促销产品折扣、运输费用、库存情况、产品及包装规格可能因各种原因有所变动，恕不另行通知，确切详情请联系上海金畔生物科技有限公司。

Met e 1 (shrimp) (rec.) 原肌球蛋白1（虾）（rec.） 品牌：AbFrontier

发表于由

Met e 1 (shrimp) (rec.)

原肌球蛋白1（虾）（rec.）

品牌：AbFrontier
CAS No.：
储存条件：-80℃
纯度：–
产品编号

(生产商编号)

 等级 规格 运输包装 零售价(RMB) 库存情况 参考值

YIF-LF-P0501A

 – 0.5 mg –

Met e 1 (shrimp) (rec.) 原肌球蛋白1（虾）（rec.） 品牌：AbFrontier

 – –


* 干冰运输、大包装及大批量的产品需酌情添加运输费用


* 零售价、促销产品折扣、运输费用、库存情况、产品及包装规格可能因各种原因有所变动，恕不另行通知，确切详情请联系上海金畔生物科技有限公司。

Met e 1 (shrimp) (rec.) 原肌球蛋白1（虾）（rec.） 品牌：AbFrontier

发表于由

Met e 1 (shrimp) (rec.)

原肌球蛋白1（虾）（rec.）

品牌：AbFrontier
CAS No.：
储存条件：-80℃
纯度：–
产品编号

(生产商编号)

 等级 规格 运输包装 零售价(RMB) 库存情况 参考值

YIF-LF-P0501B

 – 1 mg –

Met e 1 (shrimp) (rec.) 原肌球蛋白1（虾）（rec.） 品牌：AbFrontier

 – –


* 干冰运输、大包装及大批量的产品需酌情添加运输费用


* 零售价、促销产品折扣、运输费用、库存情况、产品及包装规格可能因各种原因有所变动，恕不另行通知，确切详情请联系上海金畔生物科技有限公司。

N-Formyl-Met-Leu-Phe(Synonyms: fMLP N-Formyl-MLF)

发表于由

N-Formyl-Met-Leu-Phe;(Synonyms: fMLP; N-Formyl-MLF) 纯度: 99.81%

N-Formyl-Met-Leu-Phe (fMLP; N-Formyl-MLF) 是一种趋化肽和N-甲酰基肽受体 (FPR) 的特异性配体。报道显示N-Formyl-Met-Leu-Ph 可抑制 TNF-alpha 的分泌。

N-Formyl-Met-Leu-Pheamp;;(Synonyms: fMLP; N-Formyl-MLF)

N-Formyl-Met-Leu-Phe Chemical Structure

CAS No. : 59880-97-6

规格 价格 是否有货 数量
5 mg ￥500 In-stock
10 mg ￥900 In-stock
50 mg ￥1900 In-stock
100 mg ; 询价 ;
200 mg ; 询价 ;

* Please select Quantity before adding items.

N-Formyl-Met-Leu-Phe 相关产品

bull;相关化合物库:

  • Bioactive Compound Library Plus
  • Peptidomimetic Library
  • Peptide Library

生物活性

N-Formyl-Met-Leu-Phe (fMLP; N-Formyl-MLF) is a chemotactic peptide and a specific ligand of N-formyl peptide receptor (FPR). N-Formyl-Met-Leu-Ph is reported to inhibit TNF-alpha secretion.

IC50 Target

TNF-alpha[1]
体外研究
(In Vitro)

Binding of N-Formyl-Met-Leu-Phe to its specific cell surface receptor, N-formyl peptide receptor (FPR), triggers different cascades of biochemical events, eventually leading to cellular activation. FPR is a chemoattractant receptor belonging to the G protein-coupled receptor family. N-Formyl-Met-Leu-Phe promotes osteoblastic commitment and suppresses adipogenic commitment under osteoblastic differentiation conditions. N-Formyl-Met-Leu-Phe stimulates osteogenesis is associated with increased expression of osteogenic markers and mineralization. N-Formyl-Met-Leu-Phe inhibits expression of peroxisome proliferator-activated receptor-γ1. N-Formyl-Met-Leu-Phe-stimulated osteogenic differentiation is mediated via FPR1-phospholipase C/phospholipase D-Ca2+-calmodulin-dependent kinase II-ERK-CREB signaling pathways[1]. N-Formyl-Met-Leu-Phe, a bacterial-derived peptide, induced proinflammatory cytokine gene expression in human peripheral blood monocytes. Bacterial products LPS and N-Formyl-Met-Leu-Phe synergistically induce inflammatory response via multiple signaling pathways. TLR4, IKKβ-IκBα, and NF-κB signaling pathways are involved in the synergistic induction of TNF-α via p65 nuclear translocation-dependent mechanisms[2].

MCE has not independently confirmed the accuracy of these methods. They are for reference only.
体内研究
(In Vivo)

N-Formyl-Met-Leu-Phe promotes bone formation in zebrafish and rabbits. Extensive skeletal development is evident at 5 dpf in over 80% of N-Formyl-Met-Leu-Phe-treated zebrafish. Treatment with N-Formyl-Met-Leu-Phe results in increased expression of Runx2. Bone marrow spaces are widely formed, and connective tissue covering bone is dense, like periosteum, in N-Formyl-Met-Leu-Phe-treated calvaria[1]. N-Formyl-Met-Leu-Phe mediate release of calprotectin from PMN in vitro. It induces release of calprotectin from PMN in a dose dependent manner. A minimum of 10% of total PMN calprotectin is retained at concentrations of 0.1-10.0 nM of N-Formyl-Met-Leu-Phe[3].

MCE has not independently confirmed the accuracy of these methods. They are for reference only.
分子量

437.55

Formula

C21H31N3O5S
CAS 号

59880-97-6
Sequence

Formyl-Met-Leu-Phe
Sequence Shortening

Formyl-MLF
运输条件

Room temperature in continental US; may vary elsewhere.
储存方式
Powder -80deg;C 2 years
-20deg;C 1 year
In solvent -80deg;C 6 months
-20deg;C 1 month
溶解性数据
In Vitro:;

DMSO : ≥ 82.5 mg/mL (188.55 mM)

H2O : < 0.1 mg/mL (ultrasonic) (insoluble)

* “≥” means soluble, but saturation unknown.

配制储备液
浓度 溶剂体积 质量 1 mg 5 mg 10 mg
1 mM 2.2855 mL 11.4273 mL 22.8545 mL
5 mM 0.4571 mL 2.2855 mL 4.5709 mL
10 mM 0.2285 mL 1.1427 mL 2.2855 mL

*

请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液；一旦配成溶液，请分装保存，避免反复冻融造成的产品失效。
储备液的保存方式和期限：-80°C, 6 months; -20°C, 1 month。-80°C 储存时，请在 6 个月内使用，-20°C 储存时，请在 1 个月内使用。

In Vivo:

请根据您的实验动物和给药方式选择适当的溶解方案。以下溶解方案都请先按照 In Vitro 方式配制澄清的储备液，再依次添加助溶剂：

——为保证实验结果的可靠性，澄清的储备液可以根据储存条件，适当保存；体内实验的工作液，建议您现用现配，当天使用； 以下溶剂前显示的百
分比是指该溶剂在您配制终溶液中的体积占比；如在配制过程中出现沉淀、析出现象，可以通过加热和/或超声的方式助溶

  • 1.

    请依序添加每种溶剂：;10% DMSO ;; 40% PEG300 ;; 5% Tween-80 ;; 45% saline

    Solubility: ≥ 2.08 mg/mL (4.75 mM); Clear solution

    此方案可获得 ≥ 2.08 mg/mL (4.75 mM，饱和度未知) 的澄清溶液。

    以 1 mL 工作液为例，取 100 μL 20.8 mg/mL 的澄清 DMSO 储备液加到 400 μL PEG300 中，混合均匀；向上述体系中加入50 μL Tween-80，混合均匀；然后继续加入 450 μL生理盐水定容至 1 mL。

    将 0.9 g 氯化钠，完全溶解于 100 mL ddH₂O 中，得到澄清透明的生理盐水溶液

  • 2.

    请依序添加每种溶剂：;10% DMSO ;; 90% corn oil

    Solubility: ≥ 2.08 mg/mL (4.75 mM); Clear solution

    此方案可获得 ≥ 2.08 mg/mL (4.75 mM，饱和度未知) 的澄清溶液，此方案不适用于实验周期在半个月以上的实验。

    以 1 mL 工作液为例，取 100 μL 20.8 mg/mL 的澄清 DMSO 储备液加到 900 μL玉米油中，混合均匀。

*以上所有助溶剂都可在 MCE 网站选购。
参考文献

  • [1]. Shin MK, et al. N-formyl-methionyl-leucyl-phenylalanine (fMLP) promotes osteoblast differentiation via the N-formyl peptide receptor 1-mediated signaling pathway in human mesenchymal stem cells from bone marrow. J Biol Chem. 2011 May 13;286(19):17133-43.

    [2]. Chen LY, et al. Synergistic induction of inflammation by bacterial products lipopolysaccharide and fMLP: an important microbial pathogenic mechanism. J Immunol. 2009 Feb 15;182(4):2518-24.

    [3]. Hetland G, et al. Chemotaxins C5a and fMLP induce release of calprotectin (leucocyte L1 protein) from polymorphonuclear cells in vitro. Mol Pathol. 1998 Jun;51(3):143-8.
Cell Assay
[2]

Cells are cotransfected with either a dominant negative form of IκBα or a dominant negative form of IKKβ together with the NF-κB-dependent luciferase reporter plasmid. The plasmid pCMVβ is used as a control for transfection efficiency and this is monitored via the expression of β-galactosidase. Cells are transiently transfected with plasmids using DEAE-dextran. The transfected cells are cultivated for 48 h before a 6-h incubation in medium ±N-Formyl-Met-Leu-Phe, LPS, or N-Formyl-Met-Leu-Phe/LPS. Luciferase activity is determined by using the luciferase assay kit and a Monolight 3010 luminometer[2].

MCE has not independently confirmed the accuracy of these methods. They are for reference only.
Animal Administration
[2]

Mice: N-Formyl-Met-Leu-Phe is prepared in sterile PBS. Under the anesthesia, mice are intranasally treated with LPS (0.3 mg/kg) or N-Formyl-Met-Leu-Phe (0.5 mg/kg) or N-Formyl-Met-Leu-Phe and LPS in 50 μL of sterile PBS (control), BAL is performed by cannulating the trachea with sterilized PBS, and cells from BAL fluid are stained with Wright-Giemsa stain after cytocentrifuge. For TNF-α protein release, BAL fluid is collected and secreted TNF-α is measured by ELISA as described above[2].

MCE has not independently confirmed the accuracy of these methods. They are for reference only.
参考文献

  • [1]. Shin MK, et al. N-formyl-methionyl-leucyl-phenylalanine (fMLP) promotes osteoblast differentiation via the N-formyl peptide receptor 1-mediated signaling pathway in human mesenchymal stem cells from bone marrow. J Biol Chem. 2011 May 13;286(19):17133-43.

    [2]. Chen LY, et al. Synergistic induction of inflammation by bacterial products lipopolysaccharide and fMLP: an important microbial pathogenic mechanism. J Immunol. 2009 Feb 15;182(4):2518-24.

    [3]. Hetland G, et al. Chemotaxins C5a and fMLP induce release of calprotectin (leucocyte L1 protein) from polymorphonuclear cells in vitro. Mol Pathol. 1998 Jun;51(3):143-8.

Tyr-Gly-Gly-Phe-Met-OH(Synonyms: 蛋氨酸脑啡肽 Met-Enkephalin Methionine enkephalin)

发表于由

Tyr-Gly-Gly-Phe-Met-OH;(Synonyms: 蛋氨酸脑啡肽; Met-Enkephalin; Methionine enkephalin) 纯度: 98.07%

Tyr-Gly-Gly-Phe-Met-OH 通过结合阿片样物质受体 (opioid receptor)，可调控人类免疫功能，抑制肿瘤生长。

Tyr-Gly-Gly-Phe-Met-OHamp;;(Synonyms: 蛋氨酸脑啡肽; Met-Enkephalin; Methionine enkephalin)

Tyr-Gly-Gly-Phe-Met-OH Chemical Structure

CAS No. : 58569-55-4

规格 价格 是否有货 数量
Free Sample (0.1-0.5 mg) ; Apply now ;
10;mM;*;1 mL in Water ￥550 In-stock
10 mg ￥500 In-stock
25 mg ￥950 In-stock
50 mg ￥1600 In-stock
100 mg ￥2600 In-stock
200 mg ; 询价 ;
500 mg ; 询价 ;

* Please select Quantity before adding items.

Tyr-Gly-Gly-Phe-Met-OH 相关产品

bull;相关化合物库:

  • Bioactive Compound Library Plus
  • GPCR/G Protein Compound Library
  • Neuronal Signaling Compound Library
  • Anti-Cancer Compound Library
  • Peptidomimetic Library
  • Neurotransmitter Receptor Compound Library
  • Rare Diseases Drug Library
  • Peptide Library

生物活性

Tyr-Gly-Gly-Phe-Met-OH regulates human immune function and inhibits tumor growth via binding to the opioid receptor.

IC50 Target

Opioid Receptor[1].
体外研究
(In Vitro)

Methionine enkephalin (MENK), an endogenous neuropeptide has a crucial role in both neuroendocrine and immune systems. MENK is believed to have an immunoregulatory activity to have cancer biotherapy activity by binding to the opioid receptors on immune and cancer cells. MENK may also change the tumor microenvironment by binding to opioid receptor on or in cancer cells. All of these mechanisms of action have biologic significance and potential for use in cancer immunotherapy. Furthermore, they reveal a relationship between the endocrine and immune systems[1].

MCE has not independently confirmed the accuracy of these methods. They are for reference only.
分子量

573.66

Formula

C27H35N5O7S
CAS 号

58569-55-4
Sequence

Tyr-Gly-Gly-Phe-Met
Sequence Shortening

YGGFM
中文名称

蛋氨酸脑啡肽
运输条件

Room temperature in continental US; may vary elsewhere.
储存方式
Powder -80deg;C 2 years
-20deg;C 1 year
In solvent -80deg;C 6 months
-20deg;C 1 month
溶解性数据
In Vitro:;

DMSO : ≥ 40 mg/mL (69.73 mM)

H2O : 6.67 mg/mL (11.63 mM; Need ultrasonic)

* “≥” means soluble, but saturation unknown.

配制储备液
浓度 溶剂体积 质量 1 mg 5 mg 10 mg
1 mM 1.7432 mL 8.7160 mL 17.4319 mL
5 mM 0.3486 mL 1.7432 mL 3.4864 mL
10 mM 0.1743 mL 0.8716 mL 1.7432 mL

*

请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液；一旦配成溶液，请分装保存，避免反复冻融造成的产品失效。
储备液的保存方式和期限：-80°C, 6 months; -20°C, 1 month。-80°C 储存时，请在 6 个月内使用，-20°C 储存时，请在 1 个月内使用。
参考文献

  • [1]. Zhao D, et al. Methionine enkephalin, its role in immunoregulation and cancer therapy. Int Immunopharmacol. 2016 Feb 23. pii: S1567-5769(16)30050-9.

[Sar9,Met(O2)11]-Substance P TFA

发表于由

[Sar9,Met(O2)11]-Substance P TFA; 纯度: 99.68%

[Sar9,Met(O2)11]-Substance P TFA 是一种选择性的速激肽 NK1 受体激动剂。

[Sar9,Met(O2)11]-Substance P TFAamp;;

[Sar9,Met(O2)11]-Substance P TFA Chemical Structure

规格 价格 是否有货 数量
1 mg ￥990 In-stock
5 mg ￥3900 In-stock
10 mg ￥6900 In-stock
25 mg ￥14000 In-stock
50 mg ; 询价 ;
100 mg ; 询价 ;

* Please select Quantity before adding items.

[Sar9,Met(O2)11]-Substance P TFA 相关产品

bull;相关化合物库:

  • Bioactive Compound Library Plus
  • Peptide Library

生物活性

[Sar9,Met(O2)11]-Substance P TFA is a tachykinin NK1 receptor selective agonist.

IC50 Target

NK1 receptor[1]
体外研究
(In Vitro)

[Sar9,Met(O2)11]-Substance P and septide (10-100 pmol per rat, i.c.v.) are equipotent in increasing mean arterial blood pressure (MAP) and heart rate (HR), yet they have dissimilar time-course. Both agonists increase dose-dependently face washing and sniffing while [Sar9,Met(O2)11]-Substance P is the sole to produce grooming[1].

MCE has not independently confirmed the accuracy of these methods. They are for reference only.
分子量

1507.68

Formula

C66H101N18F3O17S
Sequence

Arg-Pro-Lys-Pro-Gln-Gln-Phe-Phe-{Sar}-Leu-Met[O2]-NH2
Sequence Shortening

RPKPQQFF-{Sar}-LM[O2]-NH2
运输条件

Room temperature in continental US; may vary elsewhere.
储存方式

Sealed storage, away from moisture

Powder -80deg;C 2 years
-20deg;C 1 year

*In solvent : -80deg;C, 6 months; -20deg;C, 1 month (sealed storage, away from moisture)
溶解性数据
In Vitro:;

H2O : 100 mg/mL (66.33 mM; Need ultrasonic)

配制储备液
浓度 溶剂体积 质量 1 mg 5 mg 10 mg
1 mM 0.6633 mL 3.3164 mL 6.6327 mL
5 mM 0.1327 mL 0.6633 mL 1.3265 mL
10 mM 0.0663 mL 0.3316 mL 0.6633 mL

*

请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液；一旦配成溶液，请分装保存，避免反复冻融造成的产品失效。
储备液的保存方式和期限：-80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)。-80°C 储存时，请在 6 个月内使用，-20°C 储存时，请在 1 个月内使用。
参考文献

  • [1]. Cellier E, et al. Characterization of central and peripheral effects of septide with the use of five tachykinin NK1 receptor antagonists in the rat. Br J Pharmacol. 1999 Jun;127(3):717-28.

Phe-Met-Arg-Phe amide trifluoroacetate

发表于由

Phe-Met-Arg-Phe amide trifluoroacetate; 纯度: ge;98.0%

Phe-Met-Arg-Phe amide trifluoroacetate 是一种神经肽，在肽能神经元中激活 K+ 电流，ED50 为 23 nM，这种作用具有剂量依赖性。

Phe-Met-Arg-Phe amide trifluoroacetateamp;;

Phe-Met-Arg-Phe amide trifluoroacetate Chemical Structure

CAS No. : 159237-99-7

规格 价格 是否有货 数量
5 mg ￥950 In-stock
10 mg ￥1550 In-stock
25 mg ￥3100 In-stock
50 mg ; 询价 ;
100 mg ; 询价 ;

* Please select Quantity before adding items.

Phe-Met-Arg-Phe amide trifluoroacetate 相关产品

bull;相关化合物库:

  • Bioactive Compound Library Plus
  • Peptide Library

生物活性

Phe-Met-Arg-Phe amide trifluoroacetate is an activator of K+ current, with ED50 of 23 nM in the peptidergic caudodorsal neurons.

IC50 Target

ED50: 23 nM (K+ current)[1]
体外研究
(In Vitro)

In the molluscan central nervous system, Phe-Met-Arg-Phe amide (FMRFa) acts on K+ channels in sensory, motor-, and neuroendocrine neurones. Phe-Met-Arg-Phe amide activates a novel K+ current that is characterized by a combined voltage- and receptor-dependent gating mechanism, with both factors being necessary for opening of the channels[1]. Phe-Met-Arg-Phe amide (1 μM) significantly inhibits glucose stimulated (300 mg/dL) insulin release (p<0.005) and somatostatin release (p<0.01) from the isolated perfused pancreas. Phe-Met-Arg-Phe amide (FMRF-NH2) (1 and 10 μM) is without effect on glucagon secretion, either in low glucose (50 mg/dL), high glucose (300 mg/dL), or during arginine stimulation (5 mM)[2].

MCE has not independently confirmed the accuracy of these methods. They are for reference only.
体内研究
(In Vivo)

Phe-Met-Arg-Phe amide (FMRFamide) stimulates growth hormone secretion in conscious OVX rats. The presence of Phe-Met-Arg-Phe amide-like immunoreactivity in neuronal elements in the hypothalamus suggested a role for this in the hypothalamic control of the anterior pituitary function. The injection of 200 ng (313.8 picomoles) of FMRFamide (in 2 uL) produces a significantly increased plasma GH 15 min after injection. The GH-increasing effect of 400-800 ng (627-1255 picomoles) of FMRFamide is already developed after 5 min and lasted up to 30 min[3].

MCE has not independently confirmed the accuracy of these methods. They are for reference only.
分子量

826.81

Formula

C33H44F6N8O8S
CAS 号

159237-99-7
Sequence

Phe-Met-Arg-Phe-NH2
Sequence Shortening

FMRF-NH2
运输条件

Room temperature in continental US; may vary elsewhere.
储存方式

Sealed storage, away from moisture

Powder -80deg;C 2 years
-20deg;C 1 year

*In solvent : -80deg;C, 6 months; -20deg;C, 1 month (sealed storage, away from moisture)
溶解性数据
In Vitro:;

DMSO : 100 mg/mL (120.95 mM; Need ultrasonic)

H2O : 20 mg/mL (24.19 mM; Need ultrasonic)

配制储备液
浓度 溶剂体积 质量 1 mg 5 mg 10 mg
1 mM 1.2095 mL 6.0473 mL 12.0947 mL
5 mM 0.2419 mL 1.2095 mL 2.4189 mL
10 mM 0.1209 mL 0.6047 mL 1.2095 mL

*

请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液；一旦配成溶液，请分装保存，避免反复冻融造成的产品失效。
储备液的保存方式和期限：-80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)。-80°C 储存时，请在 6 个月内使用，-20°C 储存时，请在 1 个月内使用。

In Vivo:

请根据您的实验动物和给药方式选择适当的溶解方案。以下溶解方案都请先按照 In Vitro 方式配制澄清的储备液，再依次添加助溶剂：

——为保证实验结果的可靠性，澄清的储备液可以根据储存条件，适当保存；体内实验的工作液，建议您现用现配，当天使用； 以下溶剂前显示的百
分比是指该溶剂在您配制终溶液中的体积占比；如在配制过程中出现沉淀、析出现象，可以通过加热和/或超声的方式助溶

  • 1.

    请依序添加每种溶剂：;10% DMSO ;; 40% PEG300 ;; 5% Tween-80 ;; 45% saline

    Solubility: ≥ 2.5 mg/mL (3.02 mM); Clear solution

    此方案可获得 ≥ 2.5 mg/mL (3.02 mM，饱和度未知) 的澄清溶液。

    以 1 mL 工作液为例，取 100 μL 25.0 mg/mL 的澄清 DMSO 储备液加到 400 μL PEG300 中，混合均匀；向上述体系中加入50 μL Tween-80，混合均匀；然后继续加入 450 μL生理盐水定容至 1 mL。

    将 0.9 g 氯化钠，完全溶解于 100 mL ddH₂O 中，得到澄清透明的生理盐水溶液

  • 2.

    请依序添加每种溶剂：;10% DMSO ;; 90% (20% SBE-β-CD in saline)

    Solubility: ≥ 2.5 mg/mL (3.02 mM); Clear solution

    此方案可获得 ≥ 2.5 mg/mL (3.02 mM，饱和度未知) 的澄清溶液。

    以 1 mL 工作液为例，取 100 μL 25.0 mg/mL 的澄清 DMSO 储备液加到 900 μL 20% 的 SBE-β-CD 生理盐水水溶液中，混合均匀。

    将 2 g 磺丁基醚 β-环糊精加入 5 mL 生理盐水中，再用生理盐水定容至 10 mL，完全溶解，澄清透明
  • 3.

    请依序添加每种溶剂：;10% DMSO ;; 90% corn oil

    Solubility: ≥ 2.5 mg/mL (3.02 mM); Clear solution

    此方案可获得 ≥ 2.5 mg/mL (3.02 mM，饱和度未知) 的澄清溶液，此方案不适用于实验周期在半个月以上的实验。

    以 1 mL 工作液为例，取 100 μL 25.0 mg/mL 的澄清 DMSO 储备液加到 900 μL玉米油中，混合均匀。

*以上所有助溶剂都可在 MCE 网站选购。
参考文献

  • [1]. Kits KS, et al. Phe-Met-Arg-Phe-amide activates a novel voltage-dependent K+ current through a lipoxygenasepathway in molluscan neurones. J Gen Physiol. 1997 Nov;110(5):611-28.

    [2]. Sorenson RL, et al. Phe-met-arg-phe-amide (FMRF-NH2) inhibits insulin and somatostatin secretion and anti-FMRF-NH2 sera detects pancreatic polypeptide cells in the rat islet. Peptides. 1984 Jul-Aug;5(4):777-82.

    [3]. Ottlecz A, et al. Phe-Met-Arg-Phe-amide (FMRFamide) stimulated growth hormone secretion in conscious OVX rats. Neuropeptides. 1987 Feb-Mar;9(2):161-7.

Cell Biolabs MET-5144 Kanamycin Competitive ELISA Kit 卡那霉素竞争ELISA检测试剂盒 96 assays

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