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LBIS® 小鼠瘦素 ELISA 试剂盒 LBIS® Leptin-Mouse

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LBIS® 小鼠瘦素 ELISA 试剂盒
LBIS® Leptin-Mouse

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LBIS® Leptin-MouseLBIS® 小鼠瘦素 ELISA 试剂盒 LBIS® Leptin-Mouse

LBIS® 小鼠瘦素 ELISA 试剂盒

　　瘦素（Leptin）是一种分子量为16KD的蛋白质类激素。主要由白色脂肪组织产生，其它组织如褐色脂肪组织、胎盘、卵巢、骨骼肌、胃底部、乳腺上皮细胞、骨髓、下垂体、肝脏等也能检测到。其在大鼠和小鼠中有97％的同源性。瘦素在突变种肥胖小鼠中发现，是通过调节食欲和新陈代谢来参与至能量平衡的一种重要的激素。血液中瘦素的浓度会因为空腹和低热量饮食而降低，但进食后并不会有明显增加，只有肥胖即脂肪组织量增大的情况下才会增加，可反映身体脂肪量。瘦素进入大脑，通过抑制体内下丘脑神经肽Y（NPY）和刺鼠基因相关蛋白（AgRP）表达神经的受体，另外活化体内 α-MSH 表达神经，起到降低食欲，减少进食的作用。但是，对于严重肥胖的人，虽然他们血液中瘦素的浓度很高，但对瘦素有抗性，瘦素并不能发挥抑制食欲的作用。全身性脂肪萎缩病引起的瘦素降低或缺乏，会造成胰岛素抗性、糖尿病、脂肪肝、高甘油三酯血症等疾病。除此之外，瘦素也被认为与对动脉粥样硬化的免疫反应、能量平衡和性周期相关，与肺泡表面活性剂的产生也有关系。

◆特点

　• 短时间测定（总的反应时间：３小时）

　• 微量样品（标准操作：10 μL）可测

　• 使用对环境无害的防腐剂

　• 全部试剂均为液体，可直接使用

　• 精密的测定精度和高再现性

◆构成

 

组成

状态

容量

(A)抗体固相化 96 孔板（干燥孔板）

洗净后使用

96 wells（8×12）/1 块

(B)瘦素标准溶液（小鼠）（5,000 pg/mL）

稀释后使用

500 μL/1 瓶

(C)缓冲液

即用

60 mL/1 瓶

(D)生物素结合抗瘦素抗体

稀释后使用

200 μL/1 瓶

(E)过氧化物・抗生物素蛋白结合物

稀释后使用

200 μL/1 瓶

(F)显色液（TMB）

即用

12 mL/1 瓶

(H)反应终止液（1M H2SO4）※小心轻放

即用

12 mL/1 瓶

( I )浓缩洗净液（10×）

稀释后使用

100 mL/1 瓶

封板膜

3 张

使用说明书

1 份

◆交叉反应

　※交叉反应是浓度为 3,000 pg/mL 时的数据

动物种类

对象物质

反应性和反应率（％）

Mouse

Leptin

100

α-MSH

―

IFN-γ

―

MCH

―

TNF-α

―

Rat

Leptin

31.5

Human

Lepin

＋

＋：存在交叉反应

―：不存在交叉反应

◆样品信息

小鼠的血清、血浆

10 μL/well（标准操作）

※样品量在10～50 μL范围内可以配制

 

◆测量范围

20.6～5,000 pg/mL（样品量50 μL）

103~25,000 pg/mL（样品量10 μL）

◆Validation data

精度测试（组内变异）

样品

A

B

C

1

3818

846

405

2

3810

856

405

3

3979

842

394

4

4047

851

392

5

4046

856

420

mean

3940

850

403

SD

118

6.18

11.2

CV(%)

3.0

0.73

2.8

单位：pg/mL

重复性测试（组间变异）

测量日/样品

D

E

第０天

5007

1052

第１天

5126

1063

第２天

5069

1027

第３天

5000

1000

mean

5051

1035

SD

59.3

28.0

CV(%)

1.2

2.7

单位：pg/mL n=2

加标回收测试

样品F

添加量

实测值

回收量

回收率(%)

0.00

994.9

–

–

337

1334.0

339.1

101

1061

2099.0

1104

104

1238

2284.0

1289

104

单位：pg/mL n=2

样品G

添加量

实测值

回收量

回收率(%)

0.00

1996

–

–

2731

4741

2745

101

4682

6444

4448

95.0

5462

7717

5721

105

单位：pg/mL n=2

稀释直线性测试

用稀释缓冲液分5次连续稀释2个血清样品的测量结果，线性回归方程的R2在0.9992~0.9997之间。

欲了解更多相关产品信息，请点击文字：LBIS® 疾病相关动物模型ELISA试剂盒系列


相关资料


LBIS® 小鼠瘦素 ELISA 试剂盒 LBIS® Leptin-Mouse LBIS® 小鼠瘦素 ELISA 试剂盒 LBIS® Leptin-Mouse LBIS® 小鼠瘦素 ELISA 试剂盒 LBIS® Leptin-Mouse
说明书

ELISA试剂盒选择指南①②

 ELISA试剂盒选择指③④

参考文献



 1.

Inhibition of Gastric Inhibitory Polypeptide Receptor Signaling in Adipose Tissue Reduces Insulin Resistance and Hepatic Steatosis in High-Fat Diet-Fed Mice. Joo E, Harada N, Yamane S, Fukushima T, Taura D, Iwasaki K, Sankoda A, Shibue K, Harada T, Suzuki K, Hamasaki A, Inagaki N. Diabetes. 2017 Apr;66(4):868-879 

 2.

DNA Methylation Suppresses Leptin Gene in 3T3-L1 Adipocytes. Kuroda M, Tominaga A, Nakagawa K, Nishiguchi M, Sebe M, Miyatake Y, Kitamura T, Tsutsumi R, Harada N, Nakaya Y, Sakaue H. PLoS One. 2016 Aug 5;11(8):e0160532.

 3.

Sodium alginate prevents progression of non-alcoholic steatohepatitis and liver carcinogenesis in obese and diabetic mice. Miyazaki T, Shirakami Y, Kubota M, Ideta T, Kochi T, Sakai H, Tanaka T, Moriwaki H, Shimizu M. Oncotarget. 2016 Mar 1;7(9):10448-58.

 4.

Comparison of two Kampo medicines in a diet-induced mouse obesity model. Fengying Gao, Satoru Yokoyama, Makoto Fujimoto, Koichi Tsuneyama, Ikuo Saiki, Yutaka Shimada andYoshihiro Hayakawa. Traditional & Kampo Medicine, Volume 2, Issue 2, pages 60–66, September 2015

 5.

Effect of Keishibukuryogan on Genetic and Dietary Obesity Models. Fengying Gao, Satoru Yokoyama, Makoto Fujimoto, Koichi Tsuneyama, Ikuo Saiki, Yutaka Shimada, and Yoshihiro Hayakawa. Evid Based Complement Alternat Med. 2015; 2015: 801291.

 6.

Overexpression of the adiponectin gene mimics the metabolic and stress resistance effects of calorie restriction, but not the anti-tumor effect. Kamohara R, Yamaza H, Tsuchiya T, Komatsu T, Park S, Hayashi H, Chiba T, Mori R, Otabe S, Yamada K, Nagayasu T, Shimokawa I. Exp Gerontol. 2015 Apr;64:46-54.

 7.

Preventive effects of astaxanthin on diethylnitrosamine-induced liver tumorigenesis in C57/BL/KsJ-db/db obese mice. Ohno T, Shimizu M, Shirakami Y, Miyazaki T, Ideta T, Kochi T, Kubota M, Sakai H, Tanaka T, Moriwaki H. Hepatol Res. Jul 2015.

 8.

Sudachitin, a polymethoxylated flavone, improves glucose and lipid metabolism by increasing mitochondrial biogenesis in skeletal muscle. Tsutsumi R, Yoshida T, Nii Y, Okahisa N, Iwata S,Tsukayama M, Hashimoto R, Taniguchi Y, Sakaue H, Hosaka T, Shuto E, Sakai T. 

Nutrition & Metabolism, 11:32, Jul 2014.

 9.

Type 2 diabetic conditions in Otsuka Long-Evans Tokushima Fatty rats are ameliorated by 5-aminolevulinic acid. Sato T, Yasuzawa T, Uesaka A, Izumi Y, Kamiya A, Tsuchiya K, Kobayashi Y, Kuwahata M, Kido Y. Nutr Res. Vol.34(6), p544-551, Jun 2014.

10.

Inhibitory Effects of Japanese Herbal Medicines Sho-saiko-to and Juzen-taiho-to on Nonalcoholic Steatohepatitis in Mice. Takahashi Y, Soejima Y, Kumagai A, Watanabe M, Uozaki H, Fukusato T. PLoS One. 2014 Jan 22;9(1):e87279.

11.

Down-Regulation of Hepatic Stearoyl-CoA Desaturase-1 Expression by Fucoxanthin via Leptin Signaling in Diabetic/Obese KK-A y Mice. Beppu F., Hosokawa M., Yim M-J., Shinoda T., Miyashita K. Lipids, Vol.48(5), p449-455, May 2013.

12.

Dietary Combination of Fish Oil and Taurine Decreases Fat Accumulation and Ameliorates Blood Glucose Levels in Type 2 Diabetic/Obese KK-Ay Mice. N. Mikami., M. Hosokawa., K. Miyashita. Journal of Food Science, Vol. 77(6), pH114-H120, Jun 2012.

13.

Effects of Gametophytes of Ecklonia Kurome on the Levels of Glucose and Triacylglycerol in db/db, Prediabetic C57BL/6J and IFN-γ KO Mice. F. Dwiranti., M. Hiraoka., T. Taguchi., Y. Konishi., M. Tominaga., A. Tominaga. Int J B 64 iomed Sci, Vol.8, No.1, Mar 2012.

14.

Overexpression of FoxO1 in the Hypothalamus and Pancreas Causes Obesity and Glucose Intolerance. H.-J. Kim., M. Kobayashi., T. Sasaki., O. Kikuchi., K. Amano., T. Kitazumi., Y.-S. Lee., H. Yokota-Hashimoto., V. Y. Susanti., Y. Ido Kitamura., J. Nakae., and T. Kitamura. Endocrinology, Vol.153, No.2, p659-671, Feb 2012.

15.

Spirulina improves non-alcoholic steatohepatitis, visceral fat macrophage aggregation, and serum leptin in a mouse model of metabolic syndrome. M. Fujimoto., K. Tsuneyama., T. Fujimoto., C. Selmid., 

M. E. Gershwin., Y. Shimada. Digestive and Liver Disease, 2012.

16.

Prevention mechanisms of glucose intolerance and obesity by cacao liquor procyanidin extract in high-fat diet-fed C57BL/6 mice. Y. Yamashita., M. Okabe., M. Natsume., H. Ashida. Archives of 

Biochemistry and Biophysics, 2012.

17.

Preventive Effects of Curcumin on the Development of Azoxymethane-Induced Colonic Preneoplastic Lesions in Male C57BL/KsJ-db/db Obese Mice. M. Kubota., M. Shimizu., H. Sakai., Y. Yasuda., D. Terakura., A. Baba., T. Ohno., H. Tsurumi., T. Tanaka., H. Moriwaki. Nutrition and Cancer, Vol. 64(1), 2012.

18.

Caffeic Acid Phenethyl Ester Suppresses the Production of Adipocytokines, Leptin, Tumor Necrosis Factor -Alpha and Resistin, during Differentiation to Adipocytes in 3T3-L1 Cells. S, Juman., N, Yasui., H, Okuda., A,Ueda., H, Negishi., T, Miki. and K, Ikeda. Biological and Pharmaceutical Bulletin Vol. 34 (2011) , No.4, 490.

19.

Mate Tea(Ilex paraguariensis)Promotes Satiety and Body Weight Lowering in Mice:Involvement of Glucagon-Like Peptide-1. G, M, E, Hussein., H, Matsuda., S, Nakamura., M, Hamao., T, Akiyama., K, Tamura., and M, Yoshikawa. Biol.Pharm.Bull. Vol.34(12), p1849-1855, 2011.

产品列表
产品编号 产品名称 产品规格 产品等级 备注
631-10389  （AKRLP-011）LBIS® Mouse Leptin ELISA kit
LBIS®小鼠瘦素 ELISA试剂盒 		 96 tests – –

LBIS® 胰高血糖素样肽-1（GLP-1)（活性） ELISA试剂盒 LBIS® GLP-1(active) ELISA KIT

发表于由

LBIS® 胰高血糖素样肽-1（GLP-1)（活性） ELISA试剂盒
LBIS® GLP-1(active) ELISA KIT

  • 产品特性
  • 相关资料
  • Q&A
  • 参考文献

LBIS® GLP-1(active) ELISA KITLBIS® 胰高血糖素样肽-1（GLP-1)（活性） ELISA试剂盒 LBIS® GLP-1(active) ELISA KIT

LBIS® 胰高血糖素样肽-1（GLP-1)（活性） ELISA 试剂盒

LBIS® 胰高血糖素样肽-1（GLP-1)（活性） ELISA试剂盒 LBIS® GLP-1(active) ELISA KIT


胰高血糖素样肽-1（Glucagon-like peptide-1，GLP-1）是胰高血糖素前驱体的一部分。胰高血糖素前驱体于胰脏、小肠下部以及下丘脑中表达。该前驱体的构造中含有与糖代谢有关的各种各样的生理活性物质（胰高血糖素，肠高血糖素，胃泌酸调节素，GLP-1，GLP-2）的氨基酸序列。根据表达部位加工酶的特异性，胰脏主要分泌胰高血糖素，而小肠下部主要分泌肠高血糖素和胃泌酸调节素。GLP-1 和 GLP-2 则存在于胰高血糖素前驱体后半的结构中。GLP-1由37个氨基酸组成，有２种生物活性形式，分别为 GLP-1（7-37）和GLP-1（7-36）酰胺。两者都存在于小肠下部、胰脏和下丘脑中，GLP-1（7-36）酰胺在下丘脑中占免疫反应 GLP-1（IR-GLP-1）总量的 55-94%，在小肠中占 27-73%。但在胰脏中只有极少量存在。大部分哺乳类（如人类、大鼠、小鼠、牛、猪、狗等）的 GLP-1 结构相似。

 

GLP-1: hdeferhaegtftsdvssylegqaakefiawlvkgrg　　

GLP 1(7-37): haegtftsdvssylegqaakefiawlvkgrg

GLP 1(7-36) amide: haegtftsdvssylegqaakefiawlvkgr-NH2

 

GLP-1 与小肠上部分泌的 GIP 统称为肠促胰素。该类激素是葡萄糖浓度依赖性方式促进胰岛素分泌。同时具有抑制胃肠道蠕动和胃液分泌、抑制胰高血糖素的释放、促进生长抑素的分泌、使食欲减退，促进肠上皮细胞生长、以及外周组织促进非胰岛素依赖性的葡萄糖的消耗，并促进细胞的生长的作用。有报告指出该类激素与垂体激素的分泌也有关系。

GLP-1（7-36）酰胺在生物体内代谢迅速，DPP-IV（dipeptidyl peptidase IV）会使其失去N-末端的两个氨基酸变为 GLP-1（9-36）酰胺，GLP-1（7-37）变为 GLP-1（9-37）后会失去活性。有报告指出，体外实验中，在犬的血浆中 GLP-1（7-36）酰胺的半衰期是为 61±9 分，GLP-1（7-37）为 132±16 分。因此 GLP-1 的测定，取样的时候有必要使用 DPP-IV 抑制剂。

此外，肠促胰素中的 GIP 则是有力促进 GLP-1 分泌的激素。回肠中 GLP-1 的分泌不是食物直接刺激肠道而产生的，而是由于胆碱能和肽类的刺激所产生的。

◆特点

●　短时间测定（完全反应时间：５小时）

●　微量样品（标准操作用量：10 μL）可测

●　使用对环境无害的防腐剂

●　全部试剂均为液体，可直接使用

●　精密的测定精度和高再现性

◆构成

 

组成

状态

容量

(A) 抗体固相化 96 孔板

洗净后使用

96   wells（8×12）/1 块

(B) GLP-1标准溶液（500 pg/mL）

稀释后使用

200 μL/1 瓶

(C) 缓冲液

即用

60 mL/1 瓶

(D)生物素结合抗GLP-1抗体

稀释后使用

100 μL/1 瓶

(E) 过氧化物・抗生物素蛋白结合物

稀释后使用

100 μL/1 瓶

(F) 显色液（TMB）

即用

12 mL/1 瓶

(H) 反应终止液（1M H2SO4）※小心轻放

即用

12 mL/1 瓶

( I ) 浓缩洗净液（10×）

稀释后使用

100 mL/1 瓶

封板膜

4 张

使用说明书

1 份

◆交叉反应

※交差率是 1,000 pg/mL 浓度时的数据

动物类型

对象物质

反应性和反应率（％）

Mouse/Rat

GLP-1(7-36)amide

100

GLP-1(7-37)

<   0.1

GLP-1(1-37)

―

GLP-1(9-36)amide

―

GLP-2

―

Glucagon(1-29)

―

Insulin

―

Secretin

―

GIP

―

VIP

―

GRF

―

Bovine

Glucagon(1-29)

―

VIP

―

Porcine

Glucagon(1-29)

―

VIP

―

―：不存在交叉反应

◆样品信息

 

小鼠和大鼠的血清及血浆

10 μL/well（标准操作方法）

※测量中由于酶（DPP-IV 等）的影响，采血时请注意防止 GLP-1（7-36）酰胺的分解，再使用。

 

 

◆测定范围

 

1.56～50.0 pg/mL 【0.47～15.16 pmol/L(分子量3298）】（标准曲线范围）

7.8～250 pg/mL（样品量 10 μL）

3.9～125 pg/mL（样品量 20 μL）

◆Validation data

 

精度测试（组内变异）

 

样品

A

B

1

23.7

6.44

2

23.2

5.97

3

23.4

6.39

4

24.0

5.87

5

24.1

6.44

mean

23.7

6.22

SD

0.35

0.28

CV(%)

1.5

4.5

单位：pg/mL

 

 

重复性测试（组间变异）

 

测量日/样品

E

F

第０天

25.1

6.31

第１天

25.1

6.16

第２天

25.0

6.24

第３天

25.0

6.37

mean

25.0

6.27

SD

0.03

0.09

CV(%)

0.13

1.4

单位：pg/mL n=4

 

 

加标回收测试

 

样品C

添加量

实测值

回收量

回收率(%)

0.00

3.93

–

–

3.26

7.28

3.35

103

6.51

10.3

6.37

97.8

8.14

12.1

8.17

100

 

样品D

添加量

实测值

回收量

回收率(%)

0.00

11.8

–

–

7.16

19.1

7.30

102

14.3

25.5

13.7

95.8

21.5

32.4

20.6

95.8

 

 

稀释直线性测试

 

用稀释缓冲液分三次连续稀释2个血清样品的结果，直线回归方程的R2在0.997~0.9999之间。

相关资料


LBIS® 胰高血糖素样肽-1（GLP-1)（活性） ELISA试剂盒 LBIS® GLP-1(active) ELISA KIT LBIS® 胰高血糖素样肽-1（GLP-1)（活性） ELISA试剂盒 LBIS® GLP-1(active) ELISA KIT

ELISA试剂盒选择指南①②

 ELISA试剂盒选择指③④

参考文献

1.

Elevated hepatic DPP4 activity promotes insulin resistance and non-alcoholic fatty liver disease. Baumeier C, Schluter L, Saussenthaler S, Laeger T, Rodiger M, Alaze SA, Fritsche L, Haring HU, Stefan N, Fritsche A, Schwenk RW, Schurmann A. Mol Metab. 2017 Oct;6(10):1254-1263.

 2.

Recombinant Mouse Osteocalcin Secreted by Lactococcus lactis Promotes Glucagon-Like Peptide-1 Induction in STC-1 Cells. Namai F, Shigemori S, Sudo K, Sato T, Yamamoto Y, Nigar S, Ogita T, Shimosato T. Curr Microbiol. 2017 Sep 13.

 3.

A proliferative probiotic Bifidobacterium strain in the gut ameliorates progression of metabolic disorders via microbiota modulation and acetate elevation. Aoki R, Kamikado K, Suda W, Takii H, Mikami Y, Suganuma N, Hattori M, Koga Y. Sci Rep. 2017 Mar 2;7:43522.

 4.

Effect of miglitol on the suppression of nonalcoholic steatohepatitis development and improvement of the gut environment in a rodent model. Kishida Y, Okubo H, Ohno H, Oki K, Yoneda M. J Gastroenterol. 2017 Mar 27.

 5.

Fermented vegetable and fruit extract (OM-XŪ) stimulates murine gastrointestinal tract cells and RAW264. 7 cells in vitro and regulates liver gene expression in vivo. Wakame K, Nakata A, Sato K, Mihara Y, Takahata M, Miyake Y, Okada M, Shimomiya Y, and Komatsu K. Integr Mol Med, 2017 http://www.omx.co.jp/files/attachments/8e3f7824b69bece18acca14baf314b11.PDF

 6.

Gut commensal Bacteroides acidifaciens prevents obesity and improves insulin sensitivity in mice. Yang JY, Lee YS, Kim Y, Lee SH, Ryu S, Fukuda S, Hase K, Yang CS, Lim HS, Kim MS, Kim HM, Ahn SH, Kwon BE, Ko HJ, Kweon MN. Mucosal Immunol. 2017 Jan;10(1):104-116


 7.

An extract from pork bones containing osteocalcin improves glucose metabolism in mice by oral administration. Mizokami A, Wang D, Tanaka M, Gao J, Takeuchi H, Matsui T, Hirata M. Biosci Biotechnol Biochem. 2016 Jul 27:1-8.

 8.

Catecholamines Facilitate Fuel Expenditure and Protect Against Obesity via a Novel Network of the Gut-Brain Axis in Transcription Factor Skn-1-deficient Mice. Ushiama S, Ishimaru Y, Narukawa M, Yoshioka M, Kozuka C, Watanabe N, Tsunoda M, Osakabe N, Asakura T, Masuzaki H, Abe K. EBioMedicine. 2016 Jun;8:60-71.

 9.

Dipeptidyl peptidase 4 inhibitor reduces intimal hyperplasia in rabbit autologous jugular vein graft under poor distal runoff. Koyama A, Komori K, Otsuka R, Kajikuri J, Itoh T. J Vasc Surg. 2016 May;63(5):1360-70.

10.

Dipeptidyl peptidase-4 inhibitor, linagliptin, ameliorates endothelial dysfunction and atherogenesis in normoglycemic apolipoprotein-E deficient mice. Salim HM, Fukuda D, Higashikuni Y, Tanaka K, Hirata Y, Yagi S, Soeki T, Shimabukuro M, Sata M. Vascul Pharmacol. 2016 Apr;79:16-23.

11.

Intestinal Bile Acid Composition Modulates Prohormone Convertase 1/3 (PC1/3) Expression and Consequent GLP-1 Production in Male Mice. Morimoto K, Watanabe M, Sugizaki T, Irie J, Itoh H. Endocrinology. 2016 Mar;157(3):1071-81.

12.

Total gastrectomy-induced reductions in food intake and weight are counteracted by rikkunshito by attenuating glucagon-like peptide-1 elevation in rats. Taguchi M, Dezaki K, Koizumi M, Kurashina K, Hosoya Y, Lefor AK, Sata N, Yada T. Surgery. 2016 Jan 13. pii: S0039-6060(15)01029-6


13.

DPP-4 inhibition has beneficial effects on the heart after myocardial infarction. Akihiko Kubota, Hiroyuki Takano, Haixiu Wang, Hiroshi Hasegawa, Hiroyuki Tadokoro, Masanori Hirose, Yuka Kobaraa, Tomoko Yamada-Inagawa, Issei Komuro, Yoshio Kobayashi. Journal of Molecular and Cellular Cardiology, Volume 91, Feb. 2016, Pages 72–80

14.

The dipeptidyl peptidase IV inhibitor vildagliptin suppresses development of neuropathy in diabetic rodents: Effects on peripheral sensory nerve function, structure and molecular changes. Tsuboi K, Mizukami H, Inaba W, Baba M, Yagihashi S. J Neurochem. Volume 136, Issue 4, pages 859–870, Feb. 2016

15.

Intestinal Bile Acid Composition Modulates Prohormone Convertase 1/3 (PC1/3) Expression and Consequent GLP-1 Production in male mice. Morimoto K, Watanabe M, Sugizaki T, Irie JI, Itoh H. Endocrinology. 2016 Jan 20:en20151551.

16.

Hypoxia decreases glucagon-like peptide-1 secretion from the GLUTag cell line. Kihira Y, Burentogtokh A, Itoh M, Izawa-Ishizawa Y, Ishizawa K, Ikeda Y, Tsuchiya K, Tamaki T. Biol Pharm Bull. Vol.38(4), p514-21, 2015.

17.

Dietary obacunone supplementation stimulates muscle hypertrophy, and suppresses hyperglycemia and obesity through the TGR5 and PPARγ pathway. Horiba T, Katsukawa M, Mita M, Sato R. Biochem Biophys Res Commun. Vol.463(4), p846-52, Aug 2015.

18.

Combination of DPP-4 inhibitor and PPARγ agonist exerts protective effects on pancreatic β-cells in diabetic db/db mice through the augmentation of IRS-2 expression. Hirukawa H, Kaneto H, Shimoda M, Kimura T, Okauchi S, Obata A, Kohara K, Hamamoto S, Tawaramoto K, Hashiramoto M, Kaku K. Mol Cell Endocrinol. Jun 2015.

19.

Glucagon-like peptide-1 is specifically involved in sweet taste transmission. Takai S, Yasumatsu K, Inoue M, Iwata S, Yoshida R, Shigemura N, Yanagawa Y, Drucker DJ, Margolskee RF, Ninomiya Y. FASEB J. Vol.29(6), p2268-80, Jun 2015.

20.

Dipeptidyl-peptidase-4 inhibitor, alogliptin, attenuates arterial inflammation and neointimal formation after injury in low-density lipoprotein (LDL) receptor-deficient mice. Akita K, Isoda K, Shimada K, Daida H. J Am Heart Assoc. Vol.13;4(3):e001469, Mar 2015.

21.

Effects of sleeve gastrectomy and gastric banding on the hypothalamic feeding center in an obese rat model. Kawasaki T1, Ohta M, Kawano Y, Masuda T, Gotoh K, Inomata M, Kitano S.  Surg Today. 2015 Feb 28.

22.

Duodenal-jejunal bypass improves diabetes and liver steatosis via enhanced glucagon-like peptide-1 elicited by bile acids. Kashihara H, Shimada M, Kurita N, Sato H, Yoshikawa K, Higashijima J, Chikakiyo M, Nishi M, Takasu C. J Gastroenterol Hepatol. Vol.30(2), p308-15, Feb 2015.

23.

Mosapride citrate improves nonalcoholic steatohepatitis with increased fecal lactic acid bacteria and plasma glucagon-like peptide-1 level in a rodent model. Okubo H, Nakatsu Y, Sakoda H, Kushiyama A, Fujishiro M, Fukushima T1, Matsunaga Y, Ohno H, Yoneda M, Kamata H, Shinjo T, Iwashita M, Nishimura F, Asano T. Am J Physiol Gastrointest Liver Physiol. Vol.15;308(2), G151-8, Jan 2015.

24.

Glucagon-like peptide-1 production in the GLUTag cell line is impaired by free fatty acids via endoplasmic reticulum stress. Hayashi H, Yamada R, Das SS, Sato T, Takahashi A, Hiratsuka M, Hirasawa N. Metabolism. Vol.63(6), p800-11. Jun 2014.

25.

MK-0626, a selective DPP-4 inhibitor, attenuates hepatic steatosis in ob/ob mice. Ohyama T, Sato K, Yamazaki Y, Hashizume H, Horiguchi N, Kakizaki S, Mori M, Kusano M, Yamada M. World J Gastroenterol.Vol.20(43), p16227-35, Nov 2014.

26.

Oral administration of osteocalcin improves glucose utilization by stimulating glucagon-like peptide-1 secretion. Mizokami A, Yasutake Y, Higashi S, Kawakubo-Yasukochi T, Chishaki S, Takahashi I, Takeuchi H, Hirata M. Bone. 16;69C:68-79. Sep 2014.

27.

Dietary sweet potato (Ipomoea batatas L.) leaf extract attenuates hyperglycaemia by enhancing the secretion of glucagon-like peptide-1 (GLP-1) . Nagamine R, Ueno S, Tsubata M, Yamaguchi K, Takagaki K, Hira T, Hara H, Tsuda T. Food Funct, Vol.5(9), p2309-2316, Aug 2014.

28.

Duodenal-jejunal bypass improves diabetes and liver steatosis via enhanced glucagon-like peptide-1 elicited by bile acids. Kashihara H, Shimada M, Kurita N, Sato H, Yoshikawa K, Higashijima J, Chikakiyo M, Nishi M, Takasu C. Journal of Gastroenterology and Hepatology, Aug 2014.

29.

Deletion of Hypoxia-Inducible Factor-1α in Adipocytes Enhances Glucagon-Like Peptide-1 Secretion and Reduces Adipose Tissue Inflammation. Kihira Y, Miyake M, Hirata M, Hoshina Y, Kato K, Shirakawa H, Sakaue H, Yamano N, Izawa-Ishizawa Y, Ishizawa K, Ikeda Y, Tsuchiya K, Tamaki T, Tomita S. PLoS One. 2014 Apr 4;9(4):e93856

30.

Glucagon-like peptide-1 production in the GLUTag cell line is impaired by free fatty acids via endoplasmic reticulum stress. Hayashi H., Yamada R., Shankar Das S., Sato T., Takahashi A., Hiratsuka M., Hirasawa N. Metabolism – Clinical and Experimental, 2014.

31.

Dipeptidyl peptidase-4 inhibitor ameliorates early renal injury through its anti-inflammatory action in a rat model of type 1 diabetes. Kodera AR, Shikata K., Takatsuka T., Oda K., Miyamoto S., Kajitani N., Hirota D., Ono T., Usui HK., Makino H. Biochemical and Biophysical Research Communications, Vol.443(3), p828-833, Jan 2014.

32.

Novel insight into the distribution of L-cells in the rat intestinal tract. Hansen CF., Vrang N., Sangild PT., Jelsing J. Am J Transl Res, Vol.5(3), p347-358, 2013.

33.

Cinnamtannin A2, a Tetrameric Procyanidin, Increases GLP-1 and Insulin Secretion in Mice. Yamashita Y, Okabe M, Natsume M, Ashida H. Bioscience, Biotechnology, and Biochemistry , Vol.77(4), 2013

34.

The protective roles of GLP-1R signaling in diabetic nephropathy:possible mechanism and therapeutic potential. H.Fujita, T.Morii, H.Fujishima, T.Sato, T.Shimizu, M.Hosoba, K.Tsukiyama, T.Narita, T.Takahashi, D.J.Drucker, Y.Seino, and Y.Yamada. Kidney International, 2013

35.

Beneficial effects of vildagliptin combined with miglitol on glucose tolerance and islet morphology in diet-controlled db/db mice. K.Ishibashi., A.Hara., Y.Fujitani., T.Uchida., K.Komiya., M.Tamaki., H.Abe., T.Ogihara., A.Kanazawa., R.Kawamori and H.Watada. Biochem Biophys Res Commun, Vol.440(4), p570-575, Nov 2013.

36.

Vildagliptin preserves the mass and function of pancreatic β cells via the developmental regulation and suppression of oxidative and endoplasmic reticulum stress in a mouse model of diabetes. Hamamoto S, Kanda Y, Shimoda M, Tatsumi F, Kohara K, Tawaramoto K, Hashiramoto M and Kaku K. Diabetes, Obesity and Metabolism,Vol.15(2), p153-163, Feb 2013.

37.

Osteocalcin Induces Release of Glucagon-Like Peptide-1 and Thereby Stimulates Insulin Secretion in Mice. Mizokami A, Yasutake Y, Gao J, Matsuda M, Takahashi I, Takeuchi H and Hirata M. PLoS ONE 8(2): e57375. Feb 2013

38.

DPP4 inhibitor vildagliptin preserves β-cell mass through amelioration of endoplasmic reticulum stress in C/EBPB transgenic mice. Shinobu Shimizu, Tetsuya Hosooka, Tomokazu Matsuda, Shun-ichiro Asahara, Maki Koyanagi-Kimura, Ayumi Kanno, Alberto Bartolome, Hiroaki Etoh, Megumi Fuchita, Kyoko Teruyama, Hiroaki Takahashi, Hiroyuki Inoue, Yusuke Mieda, Naoko Hashimoto, Susumu Seino, and Yoshiaki Kido. J Mol Endocrinol, Vol.49, p125-135, Oct 2012.

39.

Effects of long-term treatment with the dipeptidyl peptidase-4 inhibitor vildagliptin on islet endocrine cells in non-obese type 2 diabetic Goto-Kakizaki rats. Inaba W, Mizukami H, Kamata K, Takahashi K, Tsuboi K and Yagihashi S. European Journal of Pharmacology,Vol.691(1-3), p297-306, Sep 2012.

40.

The DPP4 inhibitor linagliptin delays the onset of diabetes and preserves β-cell mass in non-obese diabetic mice. Jacob Jelsing, Niels Vrang, Soren B van Witteloostuijn, Michael Mark and Thomas Klein. J Endocrinol, Vol.214, p381-387, Sep 2012.

41.

Neural and humoral changes associated with the adjustable gastric band: insights from a rodent model. J Kampe, A Stefanidis, S H Lockie, W A Brown, J B Dixon, A Odoi, S J Spencer, J Raven and B J Oldfield. International Journal of Obesity, 27 Mar 2012.

42.

Neural and humoral changes associated with the adjustable gastric band: insights from a rodent model. J Kampe, A Stefanidis, S H Lockie, W A Brown, J B Dixon, A Odoi, S J Spencer, J Raven and B J Oldfield. International Journal of Obesity, 27 Mar 2012.

43.

Mate Tea(Ilex paraguariensis)Promotes Satiety and Body Weight Lowering in Mice:Involvement of Glucagon-Like Peptide-1. G, M, E, Hussein., H, Matsuda., S, Nakamura., M, Hamao., T, Akiyama., K, Tamura., and M, Yoshikawa. Biol.Pharm.Bull. Vol.34(12), p1849-1855, 2011.

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5-Hydroxy-eicosapentaenoic acid is an endogenous GPR119 agonist and enhances glucose-dependent insulin secretion. R, Kogure., K, Toyama., S, Hiyamuta., I, Kojima., S, Takeda.  Biochemical and Biophysical Research Communications Vol.416(1-2), p58-63, 2011.

45.

GLP-1 Secretion in Response to Oral and Luminal Palatinose (Isomaltulose) in Rats. T,Hira.,M,Muramatsu.,M,Okuno.and H,Hara. J Nutr Sci Vitaminol, Vol.57, p30-35, 2011.

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Trehalose prevents adipocyte hypertrophy and mitigates insulin resistance. C,Arai.,N,Arai.,A,Mizote.,K,Kohno.,K,Iwaki.,T,Hanaya.,S,Arai.,S,Ushio.,S,Fukuda. Nutrition Research, Vol.30(12), p840-848, 2010.

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Imaging exocytosis of single glucagon-like peptide-1 containing granules in a murine enteroendocrine cell line with total internal reflection fluorescent microscopy. Ohara-Imaizumi,M.,Aoyagi,K.,Akimoto,Y.,Nakamichi,Y.,Nishiwaki,C.,Kawakami,H.and Nagamatsu,S. Biochemical and Biophysical Reseach Communications, Vol.390, p16-20, 2009.

产品列表
产品编号 产品名称 产品规格 产品等级 备注
637-15129 （AKMGP-011） LBIS® GLP-1(Active) ELISA Kit
LBIS® 胰高血糖素样肽-1（GLP-1）（活性） ELISA试剂盒 		 96 tests – –

LBIS® 小鼠 C-肽 ELISA 试剂盒（U型） LBIS® C-Peptide-Mouse (U type)

发表于由

LBIS® 小鼠 C-肽 ELISA 试剂盒（U型）
LBIS® C-Peptide-Mouse (U type)

  • 产品特性
  • 相关资料
  • Q&A
  • 参考文献

LBIS® C-Peptide-Mouse (U type)LBIS® 小鼠 C-肽 ELISA 试剂盒（U型） LBIS® C-Peptide-Mouse (U type)

LBIS® 小鼠 C-肽 ELISA 试剂盒（U 型）

　　胰岛素是细胞中的单链胰岛素原合成后，形成二硫键，通过酶分解激活，裂解成Ｃ肽与胰岛素。小鼠、大鼠的胰岛素的氨基酸序列相同，但Ｃ肽部分稍有不同。小鼠Ｃ肽１是 29 个氨基酸，２是 31 个的单链肽。Ｃ肽是从胰岛素原分离后，与胰岛素一同分泌生成的。长期以来，人们一直认为Ｃ肽没有生物活性，仅在合成胰岛素过程中，保证Ａ链和Ｂ链正确折叠以及二硫键正确配对时起作用。近年，随着研究的不断深入，证明了Ｃ肽具有多种生物学作用。首先，10－9M 的Ｃ肽能与内皮细胞、肾小管上皮细胞和成纤维细胞表面的Ｇ蛋白偶联受体结合。激活细胞中钙离子依赖性的信号、激活 Na＋-K＋-ATPase、促进内皮细胞的 NO 合成、与受体的结合有立体结构特异性；与胰岛素、胰岛素原、IGF-I、-II、NPY 之间无交叉反应。而且通过对缺少Ｃ肽的Ⅰ型糖尿病患者注射Ｃ肽类药物，能起到增强骨骼肌以及皮肤的血液循环、降低肾小球超滤的风险、抑制白蛋白从尿液中的排泄、改善神经机能的作用。但对身体健康的人来说，这类药物没有此等功效。因此，建议Ⅰ型糖尿病患者可以在注射胰岛素的同时，投放Ｃ肽，有利于防止并发症的发生。

　　Ｃ肽的Ｃ末端的五肽（27-31）在与受体的结合中起着重要的作用，缺少这部分的 Des（27-31）Ｃ肽就会失去它的作用。这种五肽可以完全取代Ｃ肽和受体的结合，激活 Na+-K+ATPase。有报告指出新生大鼠中 Des（27-31）Ｃ肽的存在量约占Ｃ肽总量的37%，而在成年大鼠中只占8.5%。

　　Ｃ肽在血液中的寿命是胰岛素的好几倍。在临床上，可以通过测量Ｃ肽在血液中的浓度来观察胰岛素的合成和分泌功能。且Ｃ肽在尿液中多量排除，一定程度上与血液中Ｃ肽的平均值相关，所以也可以通过尿液来检测。

　　作为人工胰岛中胰岛素分泌的指标，Ｃ肽测量是有效的。由于在培养液中经常添加胰岛素，如果要测量培养后培养液中的胰岛素的话，就不能很好地区别出分泌的胰岛素和添加的胰岛素，必须减掉培养开始时胰岛素的量。这种情况下，如果分泌的胰岛素量过少，测量误差的影响会增大，从而不能做出正确的判断。这时候，如果测量Ｃ肽，因为Ｃ肽与胰岛素是等摩尔分泌的，所以能够正确判断分泌的胰岛素。

　　本公司的整套产品能够识别Ｃ肽１、２的交叉部分，可以测量Ｃ肽的总量。

◆特点

●　短时间测定（完全反应时间：５小时）

●　（标准用量 10 μL）可测

●　使用对环境无害的防腐剂

●　全部试剂均为液体，可直接使用

●　精密的测定密度和高再现性

◆构成

组成

状态

容量

(A) 抗体固相化   96 孔板

洗净后使用

96 wells（8×12）/1 块

(B) 标准溶液（6,000 pg/mL）

稀释后使用

500 μL/1 瓶

(C) 缓冲液

即用

60 mL/1 瓶

(D) 抗C-肽抗体生物素结合

稀释后使用

100 μL/1 瓶

(E) 过氧化物・抗生物素蛋白结合物

稀释后使用

100 μL/1 瓶

(F) 显色液（TMB）

即用

12 mL/1 瓶

(H) 反应停止液（1M   H2SO4）※小心轻放

即用

12 mL/1 瓶

( I ) 浓缩洗净液（10×）

稀释后使用

100 mL/1 瓶

封板膜

4 张

使用说明书

1 份

◆样本

小鼠的血清或血浆

10 μL/well（用本品配备的缓冲液稀释后、50 μL 分注在孔板中。）

◆测定范围

46.9~3,000 pg/mL（标准曲线范围）

234.5~15,000 pg/mL（检体量 10 μL 的时候）

◆Validation data

 

精度测试（组内变异）

 

样本

A

B

1

976

238

2

969

230

3

965

230

4

1023

235

5

977

231

6

1018

228

7

1038

229

8

995

225

mean

995

231

SD

27.7

4.10

CV(%)

2.78

1.78

单位：pg/mL

重复性测试（组间变异）

 

测量日/检体

C

D

E

第0天

1502

301

60.9

第1天

1500

302

63.8

第2天

1499

301

62.2

第3天

1501

300

58.8

mean

1500

301

61.4

SD

1.12

0.66

2.13

CV(%)

0.07

0.22

3.46

单位：pg/mL, n=4

加标回收测试

 

样本F

添加量

实测值

回收量

回收率(%)

0.00

300

–

–

265

551

250

94

398

683

382

96

531

827

527

99

单位：pg/mL, n=2

样本G

添加量

实测值

回收量

回收率(%)

0.00

58.2

–

–

28.9

86.7

28.5

99

38.6

98.2

40.0

104

77.4

139

80.8

104

单位：pg/mL, n=2

稀释直线性测试

用稀释缓冲液分三次连续稀释２个血清检体的结果，直线回归方程的 R2 是 1.00 。

欲了解更多相关产品信息，请点击文字：LBIS® 疾病相关动物模型ELISA试剂盒系列


相关资料


LBIS® 小鼠 C-肽 ELISA 试剂盒（U型） LBIS® C-Peptide-Mouse (U type) LBIS® 小鼠 C-肽 ELISA 试剂盒（U型） LBIS® C-Peptide-Mouse (U type) LBIS® 小鼠 C-肽 ELISA 试剂盒（U型） LBIS® C-Peptide-Mouse (U type)
说明书

ELISA试剂盒选择指南①②

 ELISA试剂盒选择指③④

参考文献


 1.

Inhibition of Gastric Inhibitory Polypeptide Receptor Signaling in Adipose Tissue Reduces Insulin Resistance and Hepatic Steatosis in High-Fat Diet-Fed Mice. Joo E, Harada N, Yamane S, Fukushima T, Taura D, Iwasaki K, Sankoda A, Shibue K, Harada T, Suzuki K, Hamasaki A, Inagaki N. Diabetes. 2017 Apr;66(4):868-879.


 2.

Inhibition of Gastric Inhibitory Polypeptide Receptor Signaling in Adipose Tissue Reduces Insulin

Resistance and Hepatic Steatosis in High Fat Diet-Fed Mice. Joo E, Harada N, Yamane S, Fukushima

T, Taura D, Iwasaki K, Sankoda A, Shibue K, Harada T, Suzuki K, Hamasaki A, Inagaki N. Diabetes.

2017 Jan 17.

 3.

Insulin Release from the Beta Cells in Acatalasemic Mice Is Highly Susceptible to Alloxan-Induced Oxidative Stress. Kazunori Takemoto, Wakana Doi, Ken Kataoka, Kohji Ishihara, Da-Hong Wang, Hitoshi Sugiyama, Noriyoshi Masuoka. Journal of Diabetes Mellitus, 2015, 5, 81-89

 4.

Effect of Burdock Root and the Fermented Product on Alloxan-Induced Mouse Hyperglycemia

Wakana Doi, Yumi Asada, Ayaka Ohno, Yoshiko Okuda, Shota Masuda, Ayano Matsumoto,

Chihiro Mori, Takaya Agarie, Kohji Ishihara, Takayuki Murakami & Noriyoshi Masuoka Journal of

Food Research; Vol. 4, No. 4; 201

 5.

Tissue Complex of Adult Pancreatic Duct and Vascular Endothelial Cells Promotes In Vitro Differentiation into Insulin-Producing Cells. Jun Kanamune, Chongmun Kim, Yasuhiro Iwanaga, Jorge David Rivas-Carrillo, Shoichiro Sumi, Shinji Uemoto and Kazuyuki Yokokawa. J Stem Cell Res Dev 2015, 2: 005

 6.

Anti-diabetic effect of purple corn extract on C57BL/KsJ db/db mice. Bo Huang, Zhiqiang Wang, Jong Hyuk Park, Ok Hyun Ryu, Moon Ki Choi, Jae-Yong Lee, Young-Hee Kang, and Soon Sung Lim. Nutr Res Pract. 2015 Feb;9(1):22-29.

 7.

Xanthohumol Improves Diet-induced Obesity and Fatty Liver by Suppressing Sterol Regulatory Element-binding Protein (SREBP) Activation. Miyata S, Inoue J, Shimizu M, Sato R. J Biol Chem. 2015 Aug 14;290(33):20565-79.

 8.

Effect of Aspergillus awamori-Fermented Burdock Root on Mouse Diabetes Induced by

Alloxan—Prevention of Cell Apoptosis. Kazunori Takemoto, Wakana Doi, Ayumi Zukeran, Junji Inoue, Kohji Ishihara, Noriyoshi Masuoka. Food and Nutrition Sciences, Vol.5 No.16(2014), Article ID:49228,7 pages

 9.

Engineering of pseudoislets: effect on insulin secretion activity by cell number, cell population, and microchannel networks. Kojima N, Takeuchi S, Sakai Y. Transplant Proc. Vol.46(4), p1161-65, May 2014.

10.

Evaluation of 7-O-galloyl-d-sedoheptulose, isolated from Corni Fructus, in the adipose tissue of type 2 diabetic db/db mice. Park CH., Tanaka T., Yokozawa T. Fitoterapia, Vol.89, p131-142, Sep 2013.

11.

Periaortic adipose tissue-specific activation of the renin-angiotensin system contributes to atherosclerosis development in uninephrectomized apoE-/- mice. Kawahito H., Yamada H., Irie D., Kato T., Akakabe Y., Kishida S., Takata H., Wakana N., Ogata T., Ikeda K., Ueyama T., Matoba S., Mori Y., Matsubara H. American Journal of Physiology – Heart and Circulatory Physiology, Vol.305, p667-675, Sep 2013.

12.

Effect of vitamin E on alloxan-induced mouse diabetes. Kamimura W., Doi W., Takemoto K., Ishihara K., Wang D-H., Sugiyama H., Oda S., Masuoka N. Clinical Biochemistry, Vol.46(9), p795-798, Jun 2013.

13.

Evaluation of 7-O-galloyl-d-sedoheptulose, isolated from Corni Fructus, in the adipose tissue of type 2 diabetic db/db mice. C.H.Park, T.Tanaka, T.Yokozawa. Fitoterapia,  Vol.89, p131-42, Sep 2013.

14.

Therapeutic approach for type 1 diabetes mellitus using the novel immunomodulator FTY720 (fingolimod) in combination with once-daily injection of insulin glargine in non-obese diabetic mice. T.Tsuji, M.Inoue, Y.Yoshida, T.Fujita, Y.Kaino, T.Kohno. Journal of Diabetes Investigation, Vol.3(2), p132-137, Apr 2012.

15.

Effect of vitamin E on alloxan-induced mouse diabetes. Kamimura W, Doi W, Takemoto K, Ishihara K, Wang D-H, Sugiyama H, Oda S, Masuoka N. Clinical Biochemistry, Mar 2013.

16.

Intramedullary Cavity as an Implant Site for Bioartificial Pancreas: An In Vivo Study on Diabetic Canine. Y, Kai-Chiang., W, Chang-Chin., S, Shoichiro., K, Tzong-Fu., L, Sheng-Chuan., L, Feng-Huei. Transplantation, Vol. 90(6), p604-611, Sep 2010.

17.

The in vivo performance of bioartificial pancreas in bone marrow cavity: A case report of a spontaneous diabetic feline. K, C, Yang., C, C, Wu., S, C, Lin., S, Sumi., F, H, Lin. Biochemical and Biophysical Research Communications, Vol.393(3), p362-364, Mar 2010.

18.

In vitro reprogramming of adult hepatocytes into insulin-producing cells without viral vectors . H, Motoyama., S, Ogawa., A, Kubo., S, Miwa., J, Nakayama., Y, Tagawa., S, Miyagawa. Biochemical and Biophysical Research Communications, Vol.385(1),  p123-128, Jul 2009.

19.

Efficient differentiation of insulin-producing cells from skin-derived stem cells. Guo,W.,Miao,C.,Liu,S.,Qiu,Z.,Li,J., and Duan, E. Cell Proliferation, Vol.42(1), p49-62, 2009.

20.

Enrichment of Putative Pancreatic Progenitor Cells From Mice by Sorting for Prominin1(CD133)and PDGFRb. Yuichi Hori,Miki Fukomoto,Yoshikazu Kuroda. Stem Cells;0:2008-0192v1,2008

21.

Possibility of insulin-producing cells derived from mouse embryonic stem cells for diabetes treatment. T, Ibii., H, Shimada., S, Miura., E, Fukuma., H, Sato., H, Iwata. Journal of Bioscience and Bioengineering,Vol.103(2), p140-146, Feb 2007.

22.

The dual function of hepatic SOCS3 in insulin resistance in vivo. Torisu, T., Sato, N., Yoshiga, D., Kobayashi, T., Yoshioka, T., Mori, H., Iida, M. and Yoshimura, A. Genes to Cells, 12, p143-154, 2007.

23.

Prolonged remission of diabetes by regeneration of bold italic beta cells in diabetic mice treated with recombinant adenoviral vector expressing glucagon-like peptide-1. Liu, M.J., Shin, S., Li, N., Shigehara, T., Lee, Y.S., Yoon, J.W., and Jun H.S. Molecular Therapy 15: p86-93, 2007.

24.

Possibility of insulin-producing cells derived from mouse embryonic stem cells for diabetes treatment. Ibii, T., Shimada, H, Miura, S., Fukuma, E.,Sato, H.,and Iwata,H. J Bioscience Bioengineering 103: p140-146, 2007.

25.

A human b-cell line for transplantation therapy to control type 1 diabetes. Narushima, M., Kobayashi, N., Okitsu, T., Tanaka, Y., Li, S.A., Chen, Y., Miki, A., Tanaka, K., Nakaji, S., Takei, K., Gutierrez, A.S., Rivas-Carrillo, J.D., Navarro-Alvarez, N., Jun, H.S., Westerman, K.A., Noguchi, H., Lakey, J.R.T.,, Leboulch, P., Tanaka, N., and Yoon, J.W. Nature Biotechnology, Vol. 23(10), p1274-1282, 2005.

产品列表
产品编号 产品名称 产品规格 产品等级 备注
635-07239 （AKRCP-031）LBIS® Mouse C-peptide ELISA kit (U-type)
LBIS®小鼠 C-肽 ELISA试剂盒（U型） 		 96 tests – –

LBIS® 小鼠胰岛素 ELISA 试剂盒（RTU） LBIS® Mouse Insulin ELISA KIT(RTU)

发表于由

LBIS® 小鼠胰岛素 ELISA 试剂盒（RTU）
LBIS® Mouse Insulin ELISA KIT(RTU)

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  • 相关资料
  • Q&A
  • 参考文献

LBIS® Mouse Insulin ELISA KIT(RTU)LBIS® 小鼠胰岛素 ELISA 试剂盒（RTU） LBIS® Mouse Insulin ELISA KIT(RTU)

LBIS® 小鼠胰岛素 ELISA 试剂盒（RTU）

LBIS® 小鼠胰岛素 ELISA 试剂盒（RTU） LBIS® Mouse Insulin ELISA KIT(RTU)

　　

胰岛素是由胰脏内的胰岛β细胞分泌，分子量约 5800，等电点在 5.4 左右的一种蛋白质激素。

　　A6-A11、A7-B7、A20-B-19 之间形成二硫键，在酸性溶液或者不含 Zn 离子的中性水溶液中形成二聚体，在含锌离子的中性溶液中，则形成含２个 Zn 离子的六聚体。

　　肝脏、肌肉、脂肪组织是主要的靶组织，分别有以下的作用。

肝脏：促进糖原、蛋白质、脂肪酸合成、促进糖类的摄取和利用、抑制糖异生。

肌肉：糖类、氨基酸、Ｋ细胞膜通透性增大、促进糖原、蛋白质的合成、抑制蛋白质分解。

脂肪组织：葡萄糖细胞膜通透性增大、促进脂肪酸的合成。

　　胰岛素是细胞内的合成单链胰岛素原通过二硫键结合一起形成的。在酶分解作用下被激活，Ｃ肽和胰岛素分离。

◆特点


●　测量范围广（100～12,000 pg/mL）

●　短时间测定（总的反应时间：２小时50分钟）

●　微量样品（标准操作：10 μL）可测

●　使用对环境无害的防腐剂

●　全部试剂均为液体，可直接使用

●　精密的测定精度和高再现性

●　有效期限为12个月

◆构成

组成部分

状态

容量

(A) 抗体固相化 96 孔板

洗净后使用

96 wells（8×12）/1 块

(B)胰岛素标准溶液（小鼠）

①12,000　②4,800　③2,000　④800　

⑤300　⑥100　（pg/mL）

稀释后使用

各100 μL/1 瓶

(C) 缓冲液

即用

60 mL/1 瓶

(D) 生物素结合抗胰岛素抗体

稀释后使用

12 μL/1 瓶

(E) 过氧化物・抗生物素蛋白结合物

稀释后使用

12 μL/1 瓶

(F) 显色液（TMB）

即用

12 mL/1 瓶

(H) 反应终止液（1M H2SO4）※小心轻放

即用

12 mL/1 瓶

( I ) 浓缩洗净液（10×）

稀释后使用

100 mL/1 瓶

封板膜

3 张

使用说明书

1 份

 

◆样品信息

小鼠的血清•血浆•培养液

10 μL/well（标准操作）

※血浆采血建议使用肝素处理血液

◆测量范围

100～12,000 pg/mL（标准曲线范围）

◆Validation data

 

精度测试（组内变异）

 

样品

A

B

C

1

844

1559

5348

2

831

1584

5419

3

829

1555

5377

4

826

1591

5329

5

833

1599

5299

6

841

1525

5304

mean

834

1569

5346

SD

7.04

27.9

45.9

CV(%)

0.84

1.8

0.86

单位：pg/mL

重复性测试（组间变异）

 

测量日/样品

D

E

F

第0天

442

3510

6919

第1天

441

3494

6878

第2天

441

3500

6836

第3天

435

3533

6827

mean

440

3510

6865

SD

3.45

17.0

42.0

CV(%)

0.78

0.48

0.61

单位：pg/mL n=3

  

加标回收测试

 

样品G

添加量

实测值

回收量

回收率(%)

0.00

322

–

–

150

466

144

96.0

300

613

291

97.0

600

917

595

99.2

1200

1558

1266

106

单位：pg/mL n=3



样品H

添加量

实测值

回收量

回收率(%)

0.00

1672

–

–

500

2162

490

98.0

1500

3202

1530

102

3000

4573

2901

96.7

4500

6001

4329

96.2

单位：pg/mL n=3

 

稀释直线性测试

 

用稀释缓冲液分4次连续稀释2个血清样品的测量结果，直线回归方程的R2在0.9988~0.9998之间。

相关资料


LBIS® 小鼠胰岛素 ELISA 试剂盒（RTU） LBIS® Mouse Insulin ELISA KIT(RTU) LBIS® 小鼠胰岛素 ELISA 试剂盒（RTU） LBIS® Mouse Insulin ELISA KIT(RTU) LBIS® 小鼠胰岛素 ELISA 试剂盒（RTU） LBIS® Mouse Insulin ELISA KIT(RTU)
说明书

ELISA试剂盒选择指南①②

 ELISA试剂盒选择指③④

参考文献

 1.

Deficiency of COX7RP, a mitochondrial supercomplex assembly promoting factor, lowers blood glucose level in mice. Shiba S, Ikeda K, Horie-Inoue K, Nakayama A, Tanaka T, Inoue S. Sci Rep. 2017 Aug 8;7(1):7606.

 2.

Long-term dietary nitrite and nitrate deficiency causes the metabolic syndrome, endothelial dysfunction and cardiovascular death in mice. Kina-Tanada M, Sakanashi M, Tanimoto A, Kaname T, Matsuzaki T, Noguchi K, Uchida T, Nakasone J, Kozuka C, Ishida M, Kubota H, Taira Y, Totsuka Y, Kina SI, Sunakawa H, Omura J, Satoh K, Shimokawa H, Yanagihara N, Maeda S, Ohya Y, Matsushita M, Masuzaki H, Arasaki A, Tsutsui M. Diabetologia. 2017 Jun;60(6):1138-1151. 

 3.

α-Mangostin ameliorates hepatic steatosis and insulin resistance by inhibition C-C chemokine receptor 2. Kim HM, Kim YM, Huh JH, Lee ES, Kwon MH, Lee BR, Ko HJ, Chung CH. PLoS One. 2017 Jun 9;12(6):e0179204

 4.

Long-term dietary nitrite and nitrate deficiency causes the metabolic syndrome, endothelial dysfunction and cardiovascular death in mice. Kina-Tanada M, Sakanashi M, Tanimoto A, Kaname T, Matsuzaki T, Noguchi K, Uchida T, Nakasone J, Kozuka C, Ishida M, Kubota H, Taira Y, Totsuka Y, Kina SI, Sunakawa H, Omura J, Satoh K, Shimokawa H, Yanagihara N, Maeda S, Ohya Y, Matsushita M, Masuzaki H, Arasaki A, Tsutsui M. Diabetologia. 2017 Jun;60(6):1138-1151.

 5.

Anti-diabetic effects of luteolin and luteolin-7-O-glucoside on KK-A(y) mice. Zang Y, Igarashi K, Li Y. Biosci Biotechnol Biochem. 2016 Aug;80(8):1580-6.

 6.

Insulin-Inducible SMILE Inhibits Hepatic Gluconeogenesis. Lee JM, Seo WY, Han HS, Oh KJ, Lee YS, Kim DK, Choi S, Choi BH, Harris RA, Lee CH, Koo SH, Choi HS Diabetes. 2016 Jan;65(1):62-73.

 7.

Metabolomics-based search for therapeutic agents for non-alcoholic steatohepatitis Yoshihiko Terashima, Shin Nishiumi , Akihiro Minami, Yuki Kawano, Namiko Hoshi, Takeshi Azuma, Masaru Yoshida, Archives of Biochemistry and Biophysics, Vol.555–556, p55-65, Aug 2014.

 8.

Indirect Effects of Glucagon-Like Peptide-1 Receptor Agonist Exendin-4 on the Peripheral Circadian Clocks in Mice. Hitoshi Ando,Kentarou Ushijima,Akio Fujimura PLoS One. 2013 Nov 15;8(11):e81119.

 9.

Effects of Two Types of Non-Digestible Carbohydrates on Energy Metabolism in Mice. Akiyama, Takashi; Nakatani, Sachie; Kobata, Kenji; Wada, Masahiro Journal of Chitin and Chitosan Science, Vol.2, Number 3, p223-232(10), Sep 2014.

10.

Cinnamtannin A2, a Tetrameric Procyanidin, Increases GLP-1 and Insulin Secretion in Mice. Yamashita Y, Okabe M, Natsume M, Ashida H. Bioscience, Biotechnology, and Biochemistry , Vol.77(4), 2013.

产品列表
产品编号 产品名称 产品规格 产品等级 备注
633-23919  （AKRIN-011RU）LBIS® Mouse Insulin ELISA Kit(RTU) 
LBIS® 小鼠胰岛素 ELISA试剂盒（RTU）		 96 tests – –

LBIS® 大鼠胰岛素 ELISA 试剂盒（H 型） LBIS® Insulin-Rat (H type)

发表于由

LBIS® 大鼠胰岛素 ELISA 试剂盒（H 型）
LBIS® Insulin-Rat (H type)

  • 产品特性
  • 相关资料
  • Q&A
  • 参考文献

LBIS® Insulin-Rat (H type)LBIS® 大鼠胰岛素 ELISA 试剂盒（H 型） LBIS® Insulin-Rat (H type)

LBIS® 大鼠胰岛素 ELISA 试剂盒（H 型）

　　胰岛素是由胰脏内的胰岛β细胞分泌，分子量约 5800，等电点在 5.4 左右的一种蛋白质激素。

　　A6-A11、A7-B7、A20-B-19 之间形成二硫键，在酸性溶液或者不含Zn离子的中性水溶液中形成二聚体，在含锌离子的中性溶液中，则形成含２个 Zn 离子的六聚体。

　　肝脏、肌肉、脂肪组织是主要的靶组织，分别有以下的作用。

肝脏：促进糖原、蛋白质、脂肪酸合成、促进糖类的摄取和利用、抑制糖异生。

肌肉：糖类、氨基酸、Ｋ细胞膜通透性增大、促进糖原、蛋白质的合成、抑制蛋白质分解。

脂肪组织：葡萄糖细胞膜通透性增大、促进脂肪酸的合成。

　　胰岛素是细胞内的合成单链胰岛素原通过二硫键结合一起形成的。在酶分解作用下被激活，Ｃ肽和胰岛素分离。

◆特点

 

●　有色缓冲液（蓝色）、容易确认分装后的孔

●　短时间测定（总的反应时间：３小时）

●　微量样品（标准操作：10 μL）可测

●　使用对环境无害的防腐剂

●　全部试剂均为液体，可直接使用

●　精密的测定精度和高再现性

●　操作简便，不需要特别的预处理

 

 

◆构成

 

组成

状态

容量

(A)   抗体固相化 96 孔板

洗净后使用

96   wells（8×12）/1 块

(B)   胰岛素标准溶液（大鼠）（200 ng/mL）

稀释后使用

300 μL/1 瓶

(C)   缓冲液（蓝色）

即用

60 mL/1 瓶

(D)  生物素结合抗胰岛素抗体

稀释后使用

200 μL/1 瓶

(E)   过氧化物・抗生物素蛋白结合物

稀释后使用

200 μL/1 瓶

(F)   显色液（TMB）

即用

12 mL/1 瓶

(H)  反应终止液（1M   H2SO4）※小心轻放

即用

12 mL/1 瓶

( I ) 浓缩洗净液（10×）

稀释后使用

100 mL/1 瓶

封板膜

3 张

使用说明书

1 份

◆样品信息

大鼠的血清•血浆•培养液

10 μL/well（标准操作）

※血浆采血建议使用肝素处理血液



◆测量范围

0.5～100 ng/mL（标准曲线范围）

◆Validation data

精度测试（组内变异）

 

样品

A

B

C

1

2.73

12.9

86.7

2

2.78

12.6

86.2

3

2.78

12.8

85.6

4

2.78

12.6

85.2

5

2.73

12.6

85.9

mean

2.76

12.7

85.9

SD

0.025

0.147

0.575

CV(%)

0.9

1.2

0.7

单位：ng/mL

 


重复性测试（组间变异）

 

测量日/样品

D

E

F

第０天

3.34

25.5

70.3

第１天

3.28

25.7

70.3

第２天

3.15

25.2

71.6

mean

3.26

25.5

70.7

SD

0.097

0.280

0.765

CV(%)

3.0

1.1

1.1

单位：ng/mL n=2

 

加标回收测试

 

样品G

添加量

实测值

回收量

回收率(%)

0.00

31.3

–

–

20.0

51.2

19.9

99.5

40.0

73.1

41.8

105

60.0

94.3

63.0

105

单位：ng/mL n=2


样品H

添加量

实测值

回收量

回收率(%)

0.00

1.36

–

–

1.21

2.58

1.22

101

2.46

3.75

2.39

97.2

3.46

4.64

3.28

94.8

单位：ng/mL n=2

 

稀释直线性测试

 

用稀释缓冲液分3次连续稀释2个血清样品的测量结果，直线回归方程的R2在0.9966~0.9995之间。

相关资料


LBIS® 大鼠胰岛素 ELISA 试剂盒（H 型） LBIS® Insulin-Rat (H type) LBIS® 大鼠胰岛素 ELISA 试剂盒（H 型） LBIS® Insulin-Rat (H type) LBIS® 大鼠胰岛素 ELISA 试剂盒（H 型） LBIS® Insulin-Rat (H type)
说明书

ELISA试剂盒选择指南①②

 ELISA试剂盒选择指③④

参考文献


1.

Duodenal-jejunal bypass improves diabetes and liver steatosis via enhanced glucagon-like peptide-1 elicited by bile acids. Kashihara H, Shimada M, Kurita N, Sato H, Yoshikawa K, Higashijima J, Chikakiyo M, Nishi M, Takasu C. J Gastroenterol Hepatol. Vol.30(2), p308-15, Feb 2015.

2.

Improving Effects of Narazuke Lees on Fatty Liver of Rats Induced by High-Fat and High-Cholesterol Diets. Nakasa T, Yamagami S, Tanaka T, Tanaka H, Hariu H, Okinaka O. Food Science and Technology Research, Vol.20 (2014), No. 4, p849-857.

3.

Duodenal-jejunal bypass improves diabetes and liver steatosis via enhanced glucagon-like peptide-1 elicited by bile acids. Kashihara H, Shimada M, Kurita N, Sato H, Yoshikawa K, Higashijima J, Chikakiyo M, Nishi M, Takasu C. J Gastroenterol Hepatol. Aug 2014.

4.

Effect of diets with different fat contents on the development of diabetes in female Zucker diabetic fatty rat with leptin mutation. Kohlerova, Renata; Sznapkova, Martina; Slavkovsky, Rastislav; Jiroutova, Alena. Acta Veterinaria Brno, Vol.82(3), p289-296, 2013.

5.

Regulation of oxidative stress and inflammation by hepatic adiponectin receptor 2 in an animal model of nonalcoholic steatohepatitis. Matsunami,T.,Sato,Y.,Ariga,S.,Sato,T.,Kashimura,H.,Hasegawa,Y.,Yukawa, M. Int J Clin Exp Pathol. Vol.3(5), p472-481, 2010.

产品列表
产品编号 产品名称 产品规格 产品等级 备注
637-10629 （AKRIN-010H）LBIS® Rat Insulin ELISA Kit(H-type)
LBIS® 大鼠胰岛素 ELISA试剂盒（H型） 		 96 tests – –

LBIS® 大鼠胰岛素 ELISA 试剂盒（T 型） LBIS® Insulin-Rat-T

发表于由

LBIS® 大鼠胰岛素 ELISA 试剂盒（T 型）
LBIS® Insulin-Rat-T

  • 产品特性
  • 相关资料
  • Q&A
  • 参考文献

LBIS® Insulin-Rat-TLBIS® 大鼠胰岛素 ELISA 试剂盒（T 型） LBIS® Insulin-Rat-T

LBIS® 大鼠胰岛素 ELISA 试剂盒（T 型）

　　胰岛素是由胰脏内的胰岛β细胞分泌，分子量约 5800，等电点在 5.4 左右的一种蛋白质激素。

　　A6-A11、A7-B7、A20-B-19 之间形成二硫键，在酸性溶液或者不含 Zn 离子的中性水溶液中形成二聚体，在含锌离子的中性溶液中，则形成含２个 Zn 离子的六聚体。

肝脏、肌肉、脂肪组织是主要的靶组织，分别有以下的作用。

肝脏：促进糖原、蛋白质、脂肪酸合成、促进糖类的摄取和利用、抑制糖异生。

肌肉：糖类、氨基酸、Ｋ细胞膜通透性增大、促进糖原、蛋白质的合成、抑制蛋白质分解。

脂肪组织：葡萄糖细胞膜通透性增大、促进脂肪酸的合成。

胰岛素是细胞内的合成单链胰岛素原通过二硫键结合一起形成的。在酶分解作用下被激活，Ｃ肽和胰岛素分离。

◆特点

●　短时间测定（总的反应时间：３小时）

●　微量样品（标准操作：10 μL）可测

●　使用对环境无害的防腐剂

●　全部试剂均为液体，可直接使用

●　精密的测定精度和高再现性

●　操作简便，不需要特别的预处理

●　有效期限为12个月

◆构成

组成

状态

容量

(A) 抗体固相化 96 孔板

洗净后使用

96   wells（8×12）/1 块

(B) 胰岛素标准溶液（大鼠）（200 ng/mL）

稀释后使用

25 μL/1 瓶

(C) 缓冲液

即用

60 mL/1 瓶

(D) 生物素结合抗胰岛素抗体

稀释后使用

10 μL/1 瓶

(E) 过氧化物・抗生物素蛋白结合物

稀释后使用

20 μL/1 瓶

(F) 显色液（TMB）

即用

12 mL/1 瓶

(H) 反应终止液（1M H2SO4）※小心轻放

即用

12 mL/1 瓶

( I ) 浓缩洗净液（10×）

稀释后使用

100 mL/1 瓶

封板膜

3 张

使用说明书

1 份

 

◆样品信息

大鼠的血清•血浆•培养液

10 μL/well（标准操作）

※血浆采血建议使用肝素处理血液

◆测量范围

0.156～10 ng/mL（标准曲线范围）

◆Validation data

精度测试（组内变异）

 

样品

A

B

C

D

1

0.589

1.211

2.600

4.991

2

0.568

1.228

2.600

4.971

3

0.568

1.228

2.532

5.036

4

0.557

1.211

2.538

5.026

5

0.557

1.253

2.582

4.925

6

0.578

1.220

2.563

4.880

7

0.578

1.228

2.618

5.031

8

0.536

1.228

2.618

4.885

mean

0.566

1.226

2.581

4.968

SD

0.0165

0.0131

0.0340

0.0645

CV(%)

2.92

1.07

1.32

1.30

单位：ng/mL

 

 

重复性测试（组间变异）

 

测量日/样品

E

F

G

第０天

6.74

3.31

1.16

第１天

6.69

3.25

1.22

第２天

6.23

3.21

1.21

mean

6.55

3.25

1.20

SD

0.2792

0.0479

0.0325

CV(%)

4.3

1.5

2.7

单位：ng/mL n=5

 

 

加标回收测试

 

样品H

添加量

理论值

实测值

回收率(%)

0

–

0.996

–

0.500

1.496

1.484

99.2

1.000

1.996

2.048

103

2.000

2.996

2.779

92.7

单位：ng/mL

 

样品I

添加量

理论值

实测值

回收率(%)

0

–

1.086

–

0.500

1.586

1.562

98.5

1.000

2.086

2.061

98.8

2.000

3.086

2.753

89.2

单位：ng/mL

 

样品J

添加量

理论值

实测值

回收率(%)

0

–

1.160

–

0.500

1.660

1.637

98.6

1.000

2.160

2.054

95.1

2.000

3.166

2.963

93.6

单位：ng/mL

 

 

稀释直线性测试

 

用稀释缓冲液分4次连续稀释2个血清样品的测量结果，直线回归方程的R2在0.9983~0.9992之间。

相关资料


LBIS® 大鼠胰岛素 ELISA 试剂盒（T 型） LBIS® Insulin-Rat-T LBIS® 大鼠胰岛素 ELISA 试剂盒（T 型） LBIS® Insulin-Rat-T LBIS® 大鼠胰岛素 ELISA 试剂盒（T 型） LBIS® Insulin-Rat-T
说明书

ELISA试剂盒选择指南①②

 ELISA试剂盒选择指③④

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67.

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68.

Oral administration of corn Zein hydrolysate stimulates GLP-1 and GIP secretion and improves glucose tolerance in male normal rats and Goto-Kakizaki rats. N.Higuchi, T.Hira, N.Yamada and H.Hara. Endocrinology, Jun 2013.

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Hypothalamic Brain-Derived Neurotrophic Factor Regulates Glucagon Secretion Mediated by Pancreatic Efferent Nerves. Gotoh K, Masaki T, Chiba S, Ando H, Fujiwara K, Shimasaki T, Mitsutomi K, Katsuragi I, Kakuma T, Sakata T, Yoshimatsu H. Journal of Neuroendocrinology, Vol.25(3), p302-311, Mar 2013.

72.

Effects of electrical microstimulation of peripheral sympathetic nervous fascicle on glucose uptake in rats. Sato D, Shinzawa G, Kusunoki M, Matsui T, Sasaki H, Feng Z, Nishina A, Nakamura T. Journal of Artificial Organs, Mar 2013.

73.

Improvement of erectile function by Korean red ginseng (Panax ginseng) in a male rat model of metabolic syndrome. Kim S-D, Kim Y-J, Huh J-S, Kim S-W and Sohn D-W. Asian Journal of Andrology , Feb 2013.

74.

Quercetin intake during lactation modulates the AMP-activated protein kinase pathway in the livers of adult male rat offspring programmed by maternal protein restriction. Sato S., Mukai Y., Saito T. The Journal of Nutritional Biochemistry, Vol.24(1), p118-123, Jan 2013.

75.

Reduction of reactive oxygen species ameliorates metabolism-secretion coupling in islets of diabetic GK rats by suppressing lactate overproduction. Sasaki M, Fujimoto S, Sato Y, Nishi Y, Mukai E, Yamano G, Sato H, Tahara Y, Ogura K, Nagashima K and Inagaki N. Diabetes, January 24, 2013 , In press.

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Derangement of ghrelin secretion after long-term high-fat diet feeding in rats. Sugiishi A, Kimura M, Kamiya R, Ueki S, Yoneya M, Saito Y, Saito H. Hepatology Research, 2013, In press.

 

77.

Proteomic and bioinformatic analysis of membrane proteome in type 2 diabetic mouse liver. Kim G-H, Park E C, Yun S-H, Hong Y, Lee D-G, Shin E-Y, Jung J, Kim Y H, Lee K-B, Jang I-S, Lee Z-W, Chung Y-H, Choi J-S, Cheong C, Kim S, Kim S II. PROTEOMICS, 2013, In press.

78.

A Novel Rat Model of Type 2 Diabetes: The Zucker Fatty Diabetes Mellitus ZFDM Rat. Yokoi N, Hoshino M, Hidaka S, Yoshida E, Beppu M, Hoshikawa R, Sudo K, Kawada A, Takagi S and Seino S. Journal of Diabetes Research, Vol.2013 (2013)

79.

Urinary cystatin C as a biomarker for diabetic nephropathy and its immunohistochemical localization in kidney in Zucker diabetic fatty (ZDF) rats. Togashi Y, Miyamoto Y. Experimental and Toxicologic Pathology,Available online 12 Jul 2012.

80.

Artemisia campestris leaf extract alleviates early diabetic nephropathy in rats by inhibiting protein oxidation and nitric oxide end products. Mediha S, Hamadi F, Nejla S, Yassine C, Mohamed M, Najiba Z. Pathology – Research and Practice, Vol.208(3), p157-162, Mar 2012.

81.

Fenugreek with reduced bitterness prevents diet-induced metabolic disorders in rats. Muraki E, Chiba H, Taketani K, Hoshino S, Tsuge N, Tsunoda N and Kasono K. Lipids in Health and Disease, Vol.11(58), 2012.

82.

Oral Ingestion of Aloe vera Phytosterols Alters Hepatic Gene Expression Profiles and Ameliorates Obesity-Associated Metabolic Disorders in Zucker Diabetic Fatty Rats. E. Misawa., M. Tanaka., K. Nomaguchi., K. Nabeshima., M. Yamada., T. Toida., and K. Iwatsuki. J. Agric. Food Chem., 2012, 60 (11), pp 2799-2806

83.

 Myocardial Infarction-Prone Watanabe Heritable Hyperlipidemic Rabbits with Mesenteric Fat Accumulation Are a Novel Animal Model for Metabolic Syndrome. M. Shiomi., T. Kobayashi., N. Kuniyoshi., S. Yamada., T. Ito. Pathobiology 2012;Vol. 79 No. 6 P329-338

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High-fat diet-induced reduction of peroxisome proliferator-activated receptor-γ coactivator-1α messenger RNA levels and oxidative capacity in the soleus muscle of rats with metabolic syndrome. F. Nagatomo., H. Fujino., H. Kondo., I. Takeda., K. Tsuda., A. Ishihara. Nutrition Research, Vol. 32, Issue 2, February 2012, Pages 144-151

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The effects of running exercise on oxidative capacity and PGC-1α mRNA levels in the soleus muscle of rats with metabolic syndrome. F. Nagatomo., H. Fujino., H. Kondo., M. Kouzaki., N. Gu., I. Takeda., K. Tsuda., and A. Ishihara. The Journal of Physiological Sciences, Vol. 62, Number 2 (2012), 105-114

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A Comparative Study of Gastric Banding and Sleeve Gastrectomy in an Obese Diabetic Rat Model. T. Masuda., M. Ohta., T. Hirashita., Y. Kawano., H. Egucji., K. Yada., Y. Iwashita., S. Kitano. Obesity Surgery, Published online:27 August 2011

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Site dependency of fatty acid composition in adipose triacylglycerol in rats and its absence as a result of high-fat feeding. D. Sato., T. Nakamura., K. Tsutsumi., G. Shinzawa., T. Karimata., T. Okawa., Z. Fengc., and M. Kusunoki. Metabolism.Article in Press

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Dietary fructo-oligosaccharides improve insulin sensitivity along with the suppression of adipocytokine secretion from mesenteric fat cells in rats. A. Shinoki., and H. Hara. British Journal of Nutrition.Published online :02 June 2011.

89.

Food restriction improves glucose and lipid metabolism through Sirt1 expression: A study using a new rat model with obesity and severe hypertension. K. Takemori.,T. Kimura.,N. Shirasaka.,T. Inoue.,K. Masuno., and H. Ito. Life Sciences.Vol.88, Issues 25-26, 1088-1094. 2011

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Enhanced Urinary Bladder, Liver and Colon Carcinogenesis in Zucker Diabetic Fatty Rats in a Multiorgan Carcinogenesis Bioassay: Evidence for Mechanisms Involving Activation of PI3K Signaling and Impairment of P53 on Urinary Bladder Carcinogenesis. N. Ishii., M. Wei., A. Kakehashi., K. Doi., S. Yamano., M. Inaba., and H.Wanibuchi. Journal of Toxicologic Pathology .Vol. 24 (2011) , No. 1 pp.25

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Maternal low-protein diet suppresses vascular and renal endothelial nitric oxide synthase phosphorylation in rat offspring independent of a postnatal fructose diet. S. Sato.,Y. Mukai., and T. Norikura. Journal of Developmental Origins of Health and Disease (2011), 2: 168-175

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Combined Effects of Short-term Calorie Restriction and Exercise on Insulin Action in Normal Rats. H,Y,Jiang.,T,Koike.,P,Li.,Z,H,Wang.,Y,Kawata.,Y,Oshida. Horm Metab Res 2010; 42(13): 950-954

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Dietary Hesperidin Exerts Hypoglycemic and Hypolipidemic Effects in Streptozocin-Induce Marginal Type 1 Diabetic Rats. Akiyama,S., Katsumata,S., Suzuki,K., Ishimi,Y.,Wu,J., and Uehara,M.. J Clin Biochem Nutr.January;46(1):87-92.2010

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产品编号 产品名称 产品规格 产品等级 备注
631-01479 （AKRIN-010T） LBIS® Rat Insulin ELISA Kit(T-type)
LBIS® 大鼠胰岛素 ELISA试剂盒（T型） 		 96 tests – –

LBIS® 小鼠胰岛素 ELISA 试剂盒（T 型） LBIS® Insulin-Mouse-T

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LBIS® 小鼠胰岛素 ELISA 试剂盒（T 型）
LBIS® Insulin-Mouse-T

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  • 参考文献

LBIS® Insulin-Mouse-TLBIS® 小鼠胰岛素 ELISA 试剂盒（T 型） LBIS® Insulin-Mouse-T

LBIS® 小鼠胰岛素 ELISA 试剂盒（T 型）

LBIS® 小鼠胰岛素 ELISA 试剂盒（T 型） LBIS® Insulin-Mouse-T

　　

胰岛素是由胰脏内的胰岛β细胞分泌，分子量约 5800，等电点在 5.4 左右的一种蛋白质激素。

　　A6-A11、A7-B7、A20-B-19 之间形成二硫键，在酸性溶液或者不含 Zn 离子的中性水溶液中形成二聚体，在含锌离子的中性溶液中，则形成含２个 Zn 离子的六聚体。

肝脏、肌肉、脂肪组织是主要的靶组织，分别有以下的作用。

肝脏：促进糖原、蛋白质、脂肪酸合成、促进糖类的摄取和利用、抑制糖异生。

肌肉：糖类、氨基酸、Ｋ细胞膜通透性增大、促进糖原、蛋白质的合成、抑制蛋白质分解。

脂肪组织：葡萄糖细胞膜通透性增大、促进脂肪酸的合成。

胰岛素是细胞内的合成单链胰岛素原通过二硫键结合一起形成的。在酶分解作用下被激活，Ｃ肽和胰岛素分离。

◆特点

●　短时间测定（总的反应时间：３小时）

●　微量样品（标准操作：10 μL）可测

●　使用对环境无害的防腐剂

●　全部试剂均为液体，可直接使用

●　精密的测定精度和高再现性

●　操作简便，不需要特别的预处理

●　有效期限为 12 个月

◆构成

组成部分

状态

容量

(A) 抗体固相化 96孔板

洗净后使用

96 wells（8×12）/1 块

(B) 胰岛素标准溶液（小鼠）（200 ng/mL）

稀释后使用

25 μL/1 瓶

(C) 缓冲液

即用

60 mL/1 瓶

(D) 生物素结合抗胰岛素抗体

稀释后使用

10 μL/1 瓶

(E) 过氧化物・抗生物素蛋白结合物

稀释后使用

20 μL/1 瓶

(F) 显色液（TMB）

即用

12 mL/1 瓶

(H) 反应终止液（1M   H2SO4）※小心轻放

即用

12 mL/1 瓶

( I ) 浓缩洗净液（10×）

稀释后使用

100 mL/1 瓶

封板膜

3 张

使用说明书

1 份

◆样品信息

小鼠的血清、血浆、培养液

10 μL/well（标准操作）

※血浆采血建议使用肝素处理血液

 

◆测量范围

0.156～10 ng/mL（标准曲线范围）

◆Validation data

精度测试（组内变异）

样品

A

B

C

D

mean

0.882

1.15

3.67

5.25

SD

0.0245

0.0213

0.0649

0.0792

CV(%)

2.78

1.87

1.77

1.51

单位：ng/mL n=10

 

重复性测试（组间变异）

测量日/样品

E

F

G

第０天

5.253

1.224

0.513

第１天

5.322

1.312

0.523

第２天

5.365

1.269

0.512

第３天

5.362

1.281

0.535

mean

5.326

1.272

0.521

SD

0.0520

0.0366

0.0109

CV(%)

3.31

3.76

4.65

单位：ng/mL n=3

 

加标回收测试

 

样品H

添加量

实测值

回收量

回收率(%)

0

1.350

–

–

0.25

1.593

0.243

97.2

0.50

1.841

0.491

98.2

0.75

2.065

0.715

95.3

1.00

2.299

0.949

94.9

单位：ng/mL n=3

 

样品I

添加量

实测值

回收量

回收率(%)

0

1.941

–

–

0.50

2.496

0.505

101

0.75

2.728

0.737

98.3

1.00

2.955

0.964

96.4

1.50

3.431

1.440

96.0

单位：ng/mL n=3

 

稀释直线性测试

 

用稀释缓冲液分３次连续稀释３个血清样品的测量结果，直线回归方程的 R2在 0.993~0.999 之间。

相关资料


LBIS® 小鼠胰岛素 ELISA 试剂盒（T 型） LBIS® Insulin-Mouse-T LBIS® 小鼠胰岛素 ELISA 试剂盒（T 型） LBIS® Insulin-Mouse-T LBIS® 小鼠胰岛素 ELISA 试剂盒（T 型） LBIS® Insulin-Mouse-T
说明书

ELISA试剂盒选择指南①②

 ELISA试剂盒选择指③④

参考文献



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Paraventricular NUCB2/Nesfatin-1 Supports Oxytocin and Vasopressin Neurons to Control Feeding Behavior and Fluid Balance in Male Mice. Nakata M, Gantulga D, Santoso P, Zhang B, Masuda C, Mori M, Okada T, Yada T. Endocrinology. 2016 Jun;157(6):2322-32.

43.

Oxytocin Protects against Stress-Induced Cell Death in Murine Pancreatic β-Cells. Watanabe S, Wei FY, Matsunaga T, Matsunaga N, Kaitsuka T, Tomizawa K. Sci Rep. 2016 May 4;6:25185.

44.

Loss of circadian rhythm of circulating insulin concentration induced by high-fat diet intake is associated with disrupted rhythmic expression of circadian clock genes in the liver. Honma K, Hikosaka M, Mochizuki K, Goda T. Metabolism. 2016 Apr;65(4):482-91.

45.

Protective effect of vitamin E against alloxan-induced mouse hyperglycemia. Takemoto K, Doi W, Masuoka N. Biochim Biophys Acta. 2016 Apr;1862(4):647-50.

46.

Castration influences intestinal microflora and induces abdominal obesity in high-fat diet-fed mice. Harada N, Hanaoka R, Horiuchi H, Kitakaze T, Mitani T, Inui H, Yamaji R. Sci Rep. 2016 Mar 10;6:23001.

47.

Pharmacological Inhibition of Monoacylglycerol O-Acyltransferase 2 Improves Hyperlipidemia, Obesity, and Diabetes by Change in Intestinal Fat Utilization. Take K, Mochida T, Maki T, Satomi Y, Hirayama M, Nakakariya M, Amano N, Adachi R, Sato K, Kitazaki T, Takekawa S. PLoS One. 2016 Mar 3;11(3):e0150976.

48.

GADD34-deficient mice develop obesity, nonalcoholic fatty liver disease, hepatic carcinoma and insulin resistance Naomi Nishio and Ken-ichi Isobe Sci Rep. 2015; 5: 13519.

49.

Patterns of Brain Activation and Meal Reduction Induced by Abdominal Surgery in Mice and Modulation by Rikkunshito Lixin Wang, Sachiko Mogami, Seiichi Yakabi, Hiroshi Karasawa, Chihiro Yamada, Koji Yakabi, Tomohisa Hattori, and Yvette Taché PLoS One. 2015; 10(9): e0139325.

50.

Hot water extracts of edible Chrysanthemum morifolium Ramat. exert antidiabetic effects in obese diabetic KK-Ay mice Yamamoto J, Tadaishi M, Yamane T, Oishi Y, Shimizu M, Kobayashi-Hattoria K. Bioscience, Biotechnology, and Biochemistry, Vol.79(7), 2015.

51.

Dietary obacunone supplementation stimulates muscle hypertrophy, and suppresses hyperglycemia and obesity through the TGR5 and PPARγ pathway. Horiba T, Katsukawa M, Mita M, Sato R. Biochem Biophys Res Commun. Vol.463(4), p846-52, Aug. 2015.

52.

Hepatic STAMP2 alleviates high fat diet-induced hepatic steatosis and insulin resistance. Kim HY, Park SY, Lee MH, Rho JH, Oh YJ, Jung HU, Yoo SH, Jeong NY, Lee HJ, Suh S, Seo SY, Cheong J, Jeong JS, Yoo YH. J Hepatol. Vol.63(2), p477-85, Aug 2015.

53.

Optogenetic control of insulin secretion by pancreatic β-cells in vitro and in vivo. Kushibiki T, Okawa S, Hirasawa T, Ishihara M. Gene Ther. Vol.22(7), p553-9, Jul 2015.

54.

Preventive effects of astaxanthin on diethylnitrosamine-induced liver tumorigenesis in C57/BL/KsJ-db/db obese mice. Ohno T, Shimizu M, Shirakami Y, Miyazaki T, Ideta T, Kochi T, Kubota M, Sakai H, Tanaka T, Moriwaki H. Hepatol Res. Jul 2015

55.

Effects of liquid konjac on parameters related to obesity in diet-induced obese mice. Aoe S, Kudo H, Sakurai S. Biosci Biotechnol Biochem. Vol.79(7), p1141-6, Jul 2015.

56.

Alteration of gut microbiota by vancomycin and bacitracin improves insulin resistance via glucagon-like peptide 1 in diet-induced obesity. Hwang I, Park YJ, Kim YR, Kim YN, Ka S, Lee HY, Seong JK, Seok YJ, Kim JB. FASEB J. Vol.29(6), p2397-411, Jun 2015.

57.

PRMT4 is involved in insulin secretion via the methylation of histone H3 in pancreatic β cells. Kim JK, Lim Y, Lee JO, Lee YS, Won NH, Kim H, Kim HS. J Mol Endocrinol.Vol.54(3), p315-24, Jun 2015.

58.

Hepatic NPC1L1 overexpression ameliorates glucose metabolism in diabetic mice via suppression of gluconeogenesis. Kurano M, Hara M, Satoh H, Tsukamoto K. Metabolism. Vol.64(5), p588-96, May 2015.

59.

Chronic high intake of quercetin reduces oxidative stress and induces expression of the antioxidant enzymes in the liver and visceral adipose tissues in mice. Kobori M, Takahashi Y, Akimoto Y, Sakurai M, Matsunaga I, Nishimuro H, Ippoushi K, Oike H, Ohnishi-Kameyama M. Journal of Functional Foods, Vol.15, p551–560, May 2015.

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Effects of quercetin derivatives from mulberry leaves: Improved gene expression related hepatic lipid and glucose metabolism in short-term high-fat fed mice. Sun X, Yamasaki M, Katsube T, Shiwaku K. Nutr Res Pract. Vol.9(2), p137-43, Apr 2015.

61.

Insulin Release from the Beta Cells in Acatalasemic Mice Is Highly Susceptible to Alloxan-Induced Oxidative Stress. Kazunori Takemoto, Wakana Doi, Ken Kataoka, Kohji Ishihara, Da-Hong Wang., Hitoshi Sugiyama, Noriyoshi Masuoka. JDM, Vol.5 No.2, May 2015

62.

Titanium dioxide nanoparticles increase plasma glucose via reactive oxygen species-induced insulin resistance in mice. Hu H, Guo Q, Wang C, Ma X, He H, Oh Y, Feng Y, Wu Q, Gu N. J Appl Toxicol. Mar 2015 .

63.

Compensatory hyperinsulinemia in high-fat diet-induced obese mice is associated with enhanced insulin translation in islets. Kanno A, Asahara S, Masuda K, Matsuda T, Kimura-Koyanagi M, Seino S, Ogawa W, Kido Y.  Biochem Biophys Res Commun. Vol.13;458(3), p681-6. Mar 2015.

64.

Optogenetic control of insulin secretion by pancreatic β-cells in vitro and in vivo. Kushibiki T, Okawa S, Hirasawa T, Ishihara M. Gene Ther. Mar 2015.

65.

Compensatory hyperinsulinemia in high-fat diet-induced obese mice is associated with enhanced insulin translation in islets. Kanno A, Asahara S, Masuda K, Matsuda T, Kimura-Koyanagi M, Seino S, Ogawa W, Kido Y. Biochem Biophys Res Commun. Vol.458(3), p681-6, Mar 2015.

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Essential role of mitochondrial Ca2+ uniporter in the generation of mitochondrial pH gradient and metabolism-secretion coupling in insulin-releasing cells. Quan X, Nguyen TT, Choi SK, Xu S, Das R, Cha SK, Kim N, Han J, Wiederkehr A, Wollheim CB, Park KS. J Biol Chem. Vol.290(7), p4086-96, Feb 2015.

67.

Endogenous Interleukin 18 Suppresses Hyperglycemia and Hyperinsulinemia during the Acute Phase of Endotoxemia in Mice. Yamashita H, Aoyama-Ishikawa M, Takahara M, Yamauchi C, Inoue T, Miyoshi M, Maeshige N, Usami M, Nakao A, Kotani J. Surg Infect (Larchmt). 2015 Feb;16(1):90-6.

68.

 Hot water extracts of edible Chrysanthemum morifolium Ramat. exert antidiabetic effects in obese diabetic KK-Ay mice. Junpei Yamamoto, Miki Tadaishi, Takumi Yamane, Yuichi Oishi, Makoto Shimizu & Kazuo Kobayashi-Hattoria. Bioscience, Biotechnology, and Biochemistry, Published online: 10 Feb 2015

69.

Alteration of gut microbiota by vancomycin and bacitracin improves insulin resistance via glucagon-like peptide 1 in diet-induced obesity. Hwang I, Park YJ, Kim YR1, Kim YN, Ka S, Lee HY, Seong JK, Seok YJ, Kim JB. FASEB J. Feb 2015.

70.

Titanium dioxide nanoparticles increase plasma glucose via reactive oxygen species-induced insulin resistance in mice. Hu H, Guo Q, Wang C, Ma X, He H, Oh Y, Feng Y, Wu Q, Gu N. J Appl Toxicol. Mar 2015.

71.

Ashitaba (Angelica keiskei) extract prevents adiposity in high-fat diet-fed C57BL/6 mice. Zhang T, Yamashita Y, Yasuda M, Yamamoto N, Ashida H. Food Funct. Vol.6(1), p134-144, Jan 2015.

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Dietary nitrite supplementation improves insulin resistance in type 2 diabetic KKAy mice Ohtake K, Nakano G, Ehara N, Sonoda K, Ito J, Uchida H, Kobayashi J. Nitric Oxide, Vol.44, p31–38, Jan 2015.

73.

Salicornia herbacea prevents weight gain and hepatic lipid accumulation in obese ICR mice fed a high-fat diet. Pichiah PT, Cha YS. J Sci Food Agric. Dec 2014.

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Salacia reticulata has therapeutic effects on obesity. Shimada T, Nakayama Y, Harasawa Y, Matsui H, Kobayashi H, Sai Y, Miyamoto K, Tomatsu S, Aburada M. J Nat Med. Vol.68(4), p668-676, Oct 2014.Salicornia herbacea prevents weight gain and hepatic lipid accumulation in obese ICR mice fed a high-fat diet. Pichiah PT1, Cha YS. J Sci Food Agric. Dec 2014.

75.

Ghrelin administered spinally increases the blood glucose level in mice. Sim Y-B., Park S-H., Kim S-S., Kim C-H., Kim S-J., Lim S-M., Jung J-S., Suh H-W. Peptides, Vol.54, p162-165, Apr 2014.

76.

Chronic exposure to valproic acid promotes insulin release, reduces KATP channel current and does not affect Ca2+ signaling in mouse islets. Manaka K., Nakata M., Shimomura K., Rita RS., Maejima Y., Yoshida M., Dezaki K., Kakei M., Yada T. The Journal of Physiological Sciences, Vol.64(1), p77-83, Jan 2014.

77.

Impaired Lipid and Glucose Homeostasis in Hexabromocyclododecane-Exposed Mice Fed a High-Fat Diet. Yanagisawa R., Koike E., Win-Shwe TT., Yamamoto M. and Takano H. ENVIRONMENTAL HEALTH PERSPECTIVES, Jan 2014.

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Lipid-Lowering Effects of Pediococcus acidilactici M76 Isolated from Korean Traditional Makgeolli in High Fat Diet-Induced Obese Mice. Moon Y-J., Baik S-H. and Cha Y-S. Nutrients, Vol.6(3), p1016-1028, 2014.

79.

Azilsartan, an angiotensin II type 1 receptor blocker, restores endothelial function by reducing vascular inflammation and by increasing the phosphorylation ratio Ser1177/Thr497 of endothelial nitric oxide synthase in diabetic mice. Matsumoto S., Shimabukuro M., Fukuda D., Soeki T., Yamakawa K., Masuzaki H. and Sata M. Cardiovascular Diabetology, 13:30, 2014.

80.

Intake of mulberry 1-deoxynojirimycin prevents diet-induced obesity through increases in adiponectin in mice. T.Tsuduki, I.Kikuchi, T.Kimura, K.Nakagawa, T.Miyazawa. Food Chemistry, Vol.139(1-4), p16-23, Aug 2013.

81.

Chronic treatment with novel GPR40 agonists improve whole-body glucose metabolism based on the glucose-dependent insulin secretion. H.Tanaka, S.Yoshida, H.Oshima, H.Minoura, K.Negoro, T.Yamazaki, S.Sakuda, F.Iwasaki, T.Matsui and M. Shibasaki. JPET, Jul 2013.

82.

Contribution of insulin signaling to the regulation of pancreatic beta-cell mass during the catch-up growth period in a low birth weight mouse model. Y.Yoshida, M.Fuchita, M.Kimura-Koyanagi, A.Kanno, T.Matsuda, S.Asahara, N.Hashimoto, T.Isagawa, W.Ogawa, H.Aburatani, T.Noda, S.Seino, M.Kasuga, Y.Kido. Diabetology International, Jul 2013.

83.

Differential contribution of insulin and amino acids to the mTORC1-autophagy pathway in the liver and muscle. T.Naito, A.Kuma and N.Mizushima. The Journal of Biological Chemistry, Jun 2013.

84.

Apelin Inhibits Diet-Induced Obesity by Enhancing Lymphatic and Blood Vessel Integrity. M.Sawane, K.Kajiya, H.Kidoya, M.Takagi, F.Muramatsu and N.Takakura. Diabetes, Vol.62(6), p1970-1980, Jun 2013.

85.

Ras-related C3 botulinum toxin substrate 1 (RAC1) regulates glucose-stimulated insulin secretion via modulation of F-actin. S.Asahara, Y.Shibutani, K.Teruyama, H.Y.Inoue, Y.Kawada, H.Etoh, T.Matsuda, M.Kimura-Koyanagi, N.Hashimoto, M.Sakahara, W.Fujimoto, H.Takahashi, S.Ueda, T.Hosooka, T.Satoh, H.Inoue, M.Matsumoto, A.Aiba, M.Kasuga, Y.Kido. Diabetologia, Vol.56(5), p1088-1097, May 2013.

86.

Effects of hydrophilic statins on renal tubular lipid accumulation in diet-induced obese mice. K.Gotoh, T.Masaki, S.Chiba, H.Ando, K.Fujiwara, T.Shimasaki, Y.Tawara, I.Toyooka, K.Shiraishi, K.Mitsutomi, M.Anai, E.Itateyama, J.Hiraoka, K.Aoki, N.Fukunaga, T.Nawata, T.Kakuma. Obesity Research & Clinical Practice, May 2013.

87.

Amyloid-β Induces Hepatic Insulin Resistance In Vivo via JAK2. Y.Zhang, B.Zhou, B.Deng, F.Zhang, J.Wu, Y.Wang, Y.Le and Q.Zhai. Diabetes, Vol.62(4), p1159-1166, Apr 2013．

88.

Histidine augments the suppression of hepatic glucose production by central insulin action

Kimura K, Nakamura Y, Inaba Y, Matsumoto M, Kido Y, Asahara S, Matsuda T, Watanabe H, Maeda A, Inagaki F, Mukai C, Takeda K, Akira S, Ota T, Nakabayashi H, Kaneko S, Kasuga M and Inoue H.

Diabetes, Vol.62(4), p1003-1004, Apr 2013

89.

Improved transplantation outcome through delivery of DNA encoding secretion signal peptide-linked glucagon-like peptide-1 into mouse islets

Chae H Y, Lee M, Hwang H J, Kim H A, Kang J G, Kim C S, Lee S J, Ihm S-H.

Transplant International, Vol.26(4), p443-452, Apr 2013.

90.

Histidine augments the suppression of hepatic glucose production by central insulin action

K.Kimura, Y.Nakamura, Y.Inaba, M.Matsumoto, Y.Kido, S.Asahara, T.Matsuda, H.Watanabe, A.Maeda, F.Inagaki, C.Mukai, K.Takeda, S.Akira, T.Ota, H.Nakabayashi, S.Kaneko, M.Kasuga and H.Inoue. Diabetes, Mar 2013.

91.

Wogonin ameliorates hyperglycemia and dyslipidemia via PPARα activation in db/db mice without adverse side effects. Bak E-J, Kim J-H, Lee D-E, Choi Y-H, Kim J M, Woo G-H, Cha J-H, Yoo Y-J. Clinical Nutrition, Available online 26, Mar 2013.

92.

Extracellular Signal-Regulated Kinase in the Ventromedial Hypothalamus Mediates Leptin-Induced Glucose Uptake in Red-Type Skeletal Muscle. Toda C, Shiuchi T, Kageyama H, Okamoto S, Coutinho E A, Sato T, Okamatsu-Ogura Y, Yokota S, Takagi K, Tang L, Saito K, Shioda S and Minokoshi Y. Diabetes Mar 2013.

93.

Effect of vitamin E on alloxan-induced mouse diabetes. Kamimura W, Doi W, Takemoto K, Ishihara K, Wang D-H, Sugiyama H, Oda S, Masuoka N. Clinical Biochemistry, Mar 2013.

94.

Ablation of Rnf213 retards progression of diabetes in the Akita mouse. Kobayashi H, Yamazaki S, Takashima S, Liu W, Okuda H, Yan J, Fujii Y, Hitomi T, Harada K H, Habu T, Koizumi A. Biochemical and Biophysical Research Communications, Vol.432(3), p519-525, Mar 2013.

 

95.

Hypothalamic ATF3 is involved in regulating glucose and energy metabolism in mice. Lee Y-S, Sasaki T, Kobayashi M, Kikuchi O, Kim H-J, Yokota-Hashimoto H, Shimpuku M, Susanti V-Y, Ido-Kitamura Y, Kimura K, Inoue H, Tanaka-Okamoto M, Ishizaki H, Miyoshi J, Ohya S, Tanaka Y, Kitajima S, Kitamura T. Diabetologia, Mar 2013.

96.

Ras-related C3 botulinum toxin substrate 1 (RAC1) regulates glucose-stimulated insulin secretion via modulation of F-actin. S. Asahara, Y. Shibutani, K. Teruyama, H. Y. Inoue, Y. Kawada, H. Etoh, T. Matsuda, M. Kimura-Koyanagi, N. Hashimoto, M. Sakahara, W. Fujimoto, H. Takahashi, S. Ueda, T. Hosooka, T. Satoh, H. Inoue, M. Matsumoto, A. Aiba, M. Kasuga, Y. Kido. Diabetologia, Feb 2013.

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Toll-like receptor 2 and palmitic acid cooperatively contribute to the development of nonalcoholic steatohepatitis through inflammasome activation in mice. Miura K, Yang L, Rooijen N, Brenner D A, Ohnishi H, Seki E. Hepatology, Vol.57(2), p577-589, Feb 2013.

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Transcriptional Regulatory Factor X6 (Rfx6) Increases Gastric Inhibitory Polypeptide (GIP) Expression in Enteroendocrine K-cells and Is Involved in GIP Hypersecretion in High Fat Diet-induced Obesity*. K.Suzuki, N.Harada, S.Yamane, Y.Nakamura, K.Sasaki, D.Nasteska, E.Joo, K.Shibue, T.Harada, A.Hamasaki, K.Toyoda, K.Nagashima and N.Inagaki. The Journal of Biological Chemistry, Vol.288, p1929-1938, Jan 2013.

99.

Improved hypothermic short-term storage of isolated mouse islets by adding serum to preservation solutions. Yasuko Kimura, Teru Okitsu, Liu Xibao, Hiroki Teramae, Atsuhito Okonogi, Kentaro Toyoda, Shinji Uemoto and Masanori Fukushima. Islets, Vol.5(1), Jan 2013.

100.

Anti-diabetic effect of amorphastilbol through PPARα/γ dual activation in db/db mice. Lee W, Ham J, Kwon H C, Kim Y K, Kim S-N. Biochemical and Biophysical Research Communications, Jan 2013.

101.

Transcriptional Regulatory Factor X6 (Rfx6) Increases Gastric Inhibitory Polypeptide (GIP) Expression in Enteroendocrine K-cells and Is Involved in GIP Hypersecretion in High Fat Diet-induced Obesity. Suzuki K, Harada N, Yamane S, Nakamura Y, Sasaki K, Nasteska D, Joo E, Shibue K, Harada T, Hamasaki A,Toyoda K, Nagashima K and Inagaki N. The Journal of Biological Chemistry, Vol.288, p1929-1938, Jan 2013.

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Apelin inhibits diet-induced obesity by enhancing lymphatic and blood vessel integrity. Sawane M, Kajiya K, Kidoya H, Takagi M, Muramatsu F and Takakura N. Diabetes, 2013.

103.

Beneficial effects of Allium sativum L. stem extract on lipid metabolism and antioxidant status in obese mice fed a high fat diet. Kim I, Kim H-R, Kim J-H, Om A-S. Journal of the Science of Food and Agriculture, 2013.

104.

Intake of mulberry 1-deoxynojirimycin prevents diet-induced obesity through increases in adiponectin in mice. Tsuduki T, Kikuchi I, Kimura T, Nakagawa K, Miyazawa T. Food Chemistry, Vol.139(14), p16-23, 2013.

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Effect of Mukitake mushroom (Panellus serotinus) on the pathogenesis of lipid abnormalities in obese, diabetic ob/ob mice. Inoue N, Inafuku M, Shirouchi B, Nagao K and Yanagita T. Lipids in Health and Disease, Vol.12(18), 2013.

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634-01481 （AKRIN-011T）LBIS® Insulin-Mouse-T 96 tests – –

LBIS® 大鼠胰岛素 ELISA 试剂盒（RTU） LBIS® Rat Insulin ELISA KIT(RTU)

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LBIS® 大鼠胰岛素 ELISA 试剂盒（RTU）
LBIS® Rat Insulin ELISA KIT(RTU)

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LBIS® Rat Insulin ELISA KIT(RTU)LBIS® 大鼠胰岛素 ELISA 试剂盒（RTU） LBIS® Rat Insulin ELISA KIT(RTU)

LBIS® 大鼠胰岛素 ELISA试剂盒（RTU）

　　胰岛素是由胰脏内的胰岛β细胞分泌，分子量约 5800，等电点在 5.4 左右的一种蛋白质激素。

　　A6-A11、A7-B7、A20-B-19 之间形成二硫键，在酸性溶液或者不含 Zn 离子的中性水溶液中形成二聚体，在含锌离子的中性溶液中，则形成含２个 Zn 离子的六聚体。

肝脏、肌肉、脂肪组织是主要的靶组织，分别有以下的作用。

肝脏：促进糖原、蛋白质、脂肪酸合成、促进糖类的摄取和利用、抑制糖异生。

肌肉：糖类、氨基酸、Ｋ细胞膜通透性增大、促进糖原、蛋白质的合成、抑制蛋白质分解。

脂肪组织：葡萄糖细胞膜通透性增大、促进脂肪酸的合成。

胰岛素是细胞内的合成单链胰岛素原后通过二硫键结合一起形成的。在酶分解作用下被激活，Ｃ肽和胰岛素分离。

◆特点

 

•　测量范围广（100～12,000 pg/mL）

•　短时间测定（总的反应时间：两小时五十分钟）

•　微量样品（标准操作：10 μL）可测

•　使用对环境无害的防腐剂

•　全部试剂均为液体，可直接使用

•　精密的测定精度和高再现性

•　有效期限为 12 个月

◆构成


组成

状态

容量

(A)抗体固相化 96 孔板

洗净后使用

96 wells（8×12）/1 块

(B) 胰岛素标准液（大鼠）

①12,000　②4,800　③2,000　④800　⑤300　⑥100（pg/ml）

稀释后使用

各100 μL/1 瓶

(C) 缓冲液

即用

60 mL/1 瓶

(D) 生物素结合抗胰岛素抗体

稀释后使用

12 μL/1 瓶

(E) 过氧化物・抗生物素蛋白结合物

稀释后使用

12 μL/1 瓶

(F) 显色液（TMB）

即用

12 mL/1 瓶

(H) 反应终止液（1M H2SO4）※小心轻放

即用

12 mL/1 瓶

( I ) 浓缩洗净液（10×）

稀释后使用

100 mL/1 瓶

封板膜

3 张

使用说明书

1 份

◆样品信息

大鼠的血清•血浆•培养液

10 μL/well（标准操作）

※血浆采血建议使用肝素处理血液

◆测量范围

100～12,000 pg/mL（标准曲线范围）

◆Validation data

精度测试（组内变异）

样品

A

B

C

1

798

1233

2520

2

782

1309

2601

3

783

1298

2611

4

779

1234

2598

5

788

1255

2623

6

799

1264

2642

mean

788

1266

2599

SD

8.52

32.0

42.0

CV(%)

1.1

2.5

1.6

单位：pg/mL

重复性测试（组间变异）

测量日/样品

D

E

F

第0天

516

1034

2007

第1日

514

1021

2031

第2日

510

1037

2038

第3日

528

1042

2028

mean

517

1034

2026

SD

7.58

8.9

13.3

CV(%)

1.5

0.86

0.66

单位：pg/mL n=3

加标回收测试

样品G

添加量

实测值

回收量

回收率(%)

0.00

514

–

–

150

657

143

95.3

300

803

289

96.3

600

1113

599

99.8

1200

1729

1215

101

单位：pg/mL n=3

 

样品H

添加量

实测值

回收量

回收率(%)

0.00

1223

–

–

500

1721

498

99.6

1500

2764

1541

103

3000

4161

2938

97.9

4500

5620

4397

97.7

单位：pg/mL n=3

稀释直线性测试

 

用稀释缓冲液分４次连续稀释２个血清样品的测量结果，直线回归方程的 R2 在 0.998~0.999 之间。


相关资料


LBIS® 大鼠胰岛素 ELISA 试剂盒（RTU） LBIS® Rat Insulin ELISA KIT(RTU) LBIS® 大鼠胰岛素 ELISA 试剂盒（RTU） LBIS® Rat Insulin ELISA KIT(RTU)

ELISA试剂盒选择指南①②

 ELISA试剂盒选择指③④

产品列表
产品编号 产品名称 产品规格 产品等级 备注
636-24141 （AKRIN-010RU）LBIS Rat Insulin ELISA KIT(RTU) 96 tests – –

LBIS® 小鼠胰岛素 ELISA 试剂盒（H 型） LBIS® Insulin-Mouse (H type)

发表于由

LBIS® 小鼠胰岛素 ELISA 试剂盒（H 型）
LBIS® Insulin-Mouse (H type)

  • 产品特性
  • 相关资料
  • Q&A
  • 参考文献

LBIS® Insulin-Mouse (H type)LBIS® 小鼠胰岛素 ELISA 试剂盒（H 型） LBIS® Insulin-Mouse (H type)

LBIS® 小鼠胰岛素 ELISA 试剂盒（H 型）

　　胰岛素是由胰脏内的胰岛β细胞分泌，分子量约 5800，等电点在 5.4 左右的一种蛋白质激素。

　　A6-A11、A7-B7、A20-B-19 之间形成二硫键，在酸性溶液或者不含 Zn 离子的中性水溶液中形成二聚体，在含锌离子的中性溶液中，则形成含２个 Zn 离子的六聚体。


肝脏、肌肉、脂肪组织是主要的靶组织，分别有以下的作用。

肝脏：促进糖原、蛋白质、脂肪酸合成、促进糖类的摄取和利用、抑制糖异生。

肌肉：糖类、氨基酸、Ｋ细胞膜通透性增大、促进糖原、蛋白质的合成、抑制蛋白质分解。

脂肪组织：葡萄糖细胞膜通透性增大、促进脂肪酸的合成。

胰岛素是细胞内的合成单链胰岛素原通过二硫键结合一起形成的。在酶分解作用下被激活，Ｃ肽和胰岛素分离。

◆特点

●　有色缓冲液（蓝色）、容易确认分装后的孔

●　短时间测定（总的反应时间：３小时）

●　微量样品（标准操作：10 μL）可测

●　使用对环境无害的防腐剂

●　全部试剂均为液体，可直接使用

●　精密的测定精度和高再现性

●　操作简便，不需要特别的预处理

◆构成

组成部分

状态

容量

(A) 抗体固相化 96 孔板

洗净后使用

96 wells（8×12）/1 块

(B) 胰岛素标准溶液（小鼠）（200 ng/mL）

稀释后使用

300 μL/1 瓶

(C) 缓冲液（蓝色）

即用

60 mL/1 瓶

(D) 生物素结合抗胰岛素抗体

稀释后使用

200 μL/1 瓶

(E) 过氧化物・抗生物素蛋白结合物

稀释后使用

200 μL/1 瓶

(F) 显色液（TMB）

即用

12 mL/1 瓶

(H) 反应终止液（1M H2SO4）※小心轻放

即用

12 mL/1 瓶

( I ) 浓缩洗净液（10×）

稀释后使用

100 mL/1 瓶

封板膜

3 张

使用说明书

1 份

◆样品信息

小鼠的血清、血浆、培养液

10 μL/well（标准操作）

※血浆采血建议使用肝素处理血液

 


◆测定范围


0.5～100 ng/mL（标准曲线范围）


◆Validation data

精度测试（组内变异）

 

样品

A

B

C

1

0.749

10.6

81.9

2

0.749

10.7

80.9

3

0.749

10.6

82.3

4

0.711

10.8

82.9

5

0.711

10.8

81.9

mean

0.734

10.7

82.0

SD

0.021

0.11

0.72

CV(%)

2.8

1.0

0.88

单位：ng/mL

 

重复性测试（组间变异）

 

测量日/样品

F

G

H

第0天

5.61

37.3

78.9

第1天

5.85

37.9

77.9

第2天

5.65

37.6

78.2

mean

5.70

37.6

78.3

SD

0.126

0.287

0.544

CV(%)

2.2

0.8

0.7

单位：ng/mL  n=2

 

加标回收测试

 

样品D

 

添加量

实测值

回收量

回收率(%)

0

1.10

–

–

1.0

2.16

1.06

106

2.5

3.60

2.50

100

5.1

6.06

4.96

97.3

单位：ng/mL  n=2

 

样品E

 

添加量

实测值

回收量

回收率(%)

0

31.5

–

–

20

51.5

20.0

100

40

73.1

41.6

104

60

95.1

63.6

106

单位：ng/mL n=2

 

 

稀释直线性测试

 

用稀释缓冲液分4次连续稀释2个血清样品的测量结果，直线回归方程的R2在0.9997~1.0之间。

相关资料


LBIS® 小鼠胰岛素 ELISA 试剂盒（H 型） LBIS® Insulin-Mouse (H type) LBIS® 小鼠胰岛素 ELISA 试剂盒（H 型） LBIS® Insulin-Mouse (H type) LBIS® 小鼠胰岛素 ELISA 试剂盒（H 型） LBIS® Insulin-Mouse (H type)
说明书

ELISA试剂盒选择指南①②

 ELISA试剂盒选择指③④

参考文献

1.

Does a Treadmill Running Exercise Contribute to Preventing Deterioration of Bone Mineral Density and Bone Quality of the Femur in KK-Ay Mice, a Type 2 Diabetic Animal Model? Takagi S, Yamashita T, Miura T. Calcif Tissue Int. 2017 Aug 4. 

2.

Further characterization of diabetes mellitus and body weight loss in males of the congenic mouse strain DDD. Cg-Ay. Suto J, Satou K. The Journal of Veterinary Medical Science, 2015.

3.

Fat and carbohydrate in western diet contribute differently to hepatic lipid accumulation. Wu W, Tsuchida H, Kato T, Niwa H, Horikawa Y, Takeda J, Iizuka K. Biochem Biophys Res Commun. Vol.461(4), p681-6, Jun 2015.

4.

C-C Chemokine Receptor 2 Inhibitor Ameliorates Hepatic Steatosis by Improving ER Stress and Inflammation in a Type 2 Diabetic Mouse Model. Kim HM, Lee ES, Lee BR, Yadav D, Kim YM, Ko HJ, Park KS, Lee EY, Chung CH. PLoS One. 2015 Mar 27;10(3):e0120711.

5.

Viability and functional assessment of murine pancreatic islets after transportation between Korea and Japan. Lee S, Takahashi Y, Lee KM, Mizuno M, Nemeno JG, Takebe T, Lee JI. Transplant Proc. Vol.47(3), p738-41, Apr 2015.

6.

Calorie restriction-mediated restoration of hypothalamic signal transducer and activator of transcription 3 (STAT3) phosphorylation is not effective for lowering the body weight set point in IRS-2 knockout obese mice. Satoko Senda,  Atsushi Inoue,  Arshad Mahmood,  Ryo Suzuki,  Nozomu Kamei,  Naoto Kubota,  Taku Watanabe,  Masashi Aoyama,  Allah Nawaz,  Yoshiaki Ohkuma,  Koichi Tsuneyama,  Yukiko Koshimizu,  Isao Usui,  Kumiko Saeki,  Takashi Kadowaki,  Kazuyuki Tobe. Diabetology International February 2015

7.

Overweight in Mice and Enhanced Adipogenesis In Vitro are Associated with Lack of the Hedgehog Coreceptor Boc. Lee HJ, Jo SB, Romer AI, Lim HJ, Kim MJ, Koo SH, Krauss RS, Kang JS. Diabetes. 2015 Jan 9.

8.

Investigating the suspension culture on aggregation and function of mouse pancreatic β-cellsK.-C.Yang, C.-C.Wu, S.-H.Yang, C.-C.Chiu, S.Sumi, H.-S. Lee. Journal of Biomedical Materials Research Part A, Vol.101A(8), p2273-2282, Aug 2013.

9.

Microenvironment-regulated gene expression, morphology, and in vivo performance of mouse pancreatic β-cells. Chen P-Y., Wu C-C., Lu D-H., Sumi S., Lin F-H., Yange K-C. Process Biochemistry, Vol.48(1), p58-67, Jan 2013.

10.

The anti-ulcer agent, irsogladine, increases insulin secretion by MIN6 cells. T.Matsumoto，K.Sakurai, A.Tanaka, T.Ishibashi, K.Tachibana, K.Ishikawa, K.Yokote. European Journal of Pharmacology, Vol.685(1-3), p213-217, Jun 2012.

11.

Cell coupling regulates Ins1, Pdx-1 and MafA to promote insulin secretion in mouse pancreatic beta cells. K.-C. Yanga., Z. Qi., G. Yanai., Y. Shirouza., D.-H. Lu., H.-S. Lee., S. Sumi. Process Biochemistry, Vol.46(9), p1853-1860, 2011.

12.

Dietary Combination of Fish Oil and Taurine Decreases Fat Accumulation and Ameliorates Blood Glucose Levels in Type 2 Diabetic/Obese KK-Ay Mice. N. Mikami., M. Hosokawa., K. Miyashita. Journal of Food Science, Vol. 77(6), pH114-H120, Jun 2012.

13.

Cell coupling regulates Ins1, Pdx-1 and MafA to promote insulin secretion in mouse pancreatic beta cells. K.C. Yang., Z. Qi., G. Yanai., Y. Shirouza., D.H. Lu., H.S. Lee., and S. Sumi. Process Biochemistry.

产品列表
产品编号 产品名称 产品规格 产品等级 备注
634-10379 （AKRIN-011H）LBIS® Mouse Insulin ELISA Kit(H-type)
LBIS® 小鼠胰岛素 ELISA试剂盒（H型）		 96 tests – –

LBIS® 猪胰岛素 ELISA 试剂盒 LBIS® Insulin-Porcine

发表于由

LBIS® 猪胰岛素 ELISA 试剂盒
LBIS® Insulin-Porcine

  • 产品特性
  • 相关资料
  • Q&A
  • 参考文献

LBIS® Insulin-PorcineLBIS® 猪胰岛素 ELISA 试剂盒 LBIS® Insulin-Porcine

LBIS® 猪胰岛素 ELISA 试剂盒

胰岛素是由胰脏内的胰岛β细胞分泌，分子量约 5800，等电点在 5.4 左右的一种蛋白质激素。

A6-A11、A7-B7、A20-B-19 之间形成二硫键，在酸性溶液或者不含 Zn 离子的中性水溶液中形成二聚体，在含锌离子的中性溶液中，则形成含２个 Zn 离子的六聚体。

肝脏、肌肉、脂肪组织是主要的靶组织，分别有以下的作用。

肝脏：促进糖原、蛋白质、脂肪酸合成、促进糖类的摄取和利用、抑制糖异生。

肌肉：糖类、氨基酸、Ｋ细胞膜通透性增大、促进糖原、蛋白质的合成、抑制蛋白质分解。

脂肪组织：葡萄糖细胞膜通透性增大、促进脂肪酸的合成。

胰岛素是细胞内的合成单链胰岛素原通过二硫键结合一起形成的。在酶分解作用下被激活，Ｃ肽和胰岛素分离。

◆特点

●　短时间测定（总的反应时间：３小时）

●　微量样品（标准操作：10 μL）可测

●　使用对环境无害的防腐剂

●　全部试剂均为液体，可直接使用

●　精密的测定精度和高再现性

●　操作简便，不需要特别的预处理

◆构成

组成

状态

容量

(A)   抗体固相化 96 孔板

洗净后使用

96 wells（8×12）/1 块

(B) 胰岛素标准液（猪）（240 ng/mL）

稀释后使用

25 μL/1 瓶

(C) 缓冲液

即用

60 mL/1 瓶

(D) 生物素结合抗胰岛素抗体

稀释后使用

10 μL/1 瓶

(E) 过氧化物・抗生物素蛋白结合物

稀释后使用

20 μL/1 瓶

(F) 显色液（TMB）

即用

12 mL/1瓶

(H) 反应终止液（1M H2SO4）※小心轻放

即用

12 mL/1瓶

( I ) 浓缩洗净液（10×）

稀释后使用

100 mL/1瓶

封板膜

3 张

使用说明书

1 份

◆样品信息

猪的血清•血浆•培养液

10 μL/well（标准操作）

※血浆采血建议使用肝素处理血液

 

 

◆测定范围

0.188～12 ng/mL（标准曲线范围）

◆Validation data

精度测试（组内变异）

样品

A

B

C

mean

0.991

0.482

0.201

SD

0.0321

0.0175

0.0099

CV(%)

3.2

3.6

4.9

单位：ng/mL，n=5

重复性测试（组间变异）

测量日/样品

D

E

F

mean

1.452

0.901

0.346

SD

0.0562

0.0321

0.0162

CV(%)

3.9

3.6

4.7

3天内　单位：ng/mL，n=2

加标回收测试


样品G

添加量

实测值

回收量

回收率(%)

–

0.343

–

–

0.25

0.576

0.233

93.2

0.5

0.802

0.459

91.7

1

1.295

0.952

95.2

2

2.301

1.958

97.9

单位：ng/mL

样品H

添加量

实测值

回收量

回收率(%)

–

0.202

–

–

0.25

0.437

0.235

93.8

0.5

0.666

0.464

92.8

1

1.201

0.999

99.9

2

2.240

2.038

102

单位：ng/mL

相关资料


LBIS® 猪胰岛素 ELISA 试剂盒 LBIS® Insulin-Porcine LBIS® 猪胰岛素 ELISA 试剂盒 LBIS® Insulin-Porcine LBIS® 猪胰岛素 ELISA 试剂盒 LBIS® Insulin-Porcine
说明书

ELISA试剂盒选择指南①②

 ELISA试剂盒选择指③④

产品列表
产品编号 产品名称 产品规格 产品等级 备注
630-01461 （AKRIN-013T）LBIS® Insulin-Porcine 96 tests – –