Room temperature in continental US; may vary elsewhere.
储存方式
Powder
-80deg;C
2 years
-20deg;C
1 year
In solvent
-80deg;C
6 months
-20deg;C
1 month
Solvent Solubility
In Vitro:;
H2O
Peptide Solubility and Storage Guidelines:
1.;;Calculate the length of the peptide.
2.;;Calculate the overall charge of the entire peptide according to the following table:
;
Contents
Assign value
Acidic amino acid
Asp (D), Glu (E), and the C-terminal -COOH.
-1
Basic amino acid
Arg (R), Lys (K), His (H), and the N-terminal -NH2
+1
Neutral amino acid
Gly (G), Ala (A), Leu (L), Ile (I), Val (V), Cys (C), Met (M), Thr (T), Ser (S), Phe (F), Tyr (Y), Trp (W), Pro (P), Asn (N), Gln (Q)
0
3.;;Recommended solution:
Overall charge of peptide
Details
Negative (lt;0)
1.;;Try to dissolve the peptide in water first. 2.;;If water fails, add NH4OH (lt;50 μL). 3.;;If the peptide still does not dissolve, add DMSO (50-100 μL) to solubilize the peptide.
Positive (gt;0)
1.;;Try to dissolve the peptide in water first. 2.;;If water fails, try dissolving the peptide in a 10%-30% acetic acid solution. 3.;;If the peptide still does not dissolve, try dissolving the peptide in a small amount of DMSO.
Zero (=0)
1.;;Try to dissolve the peptide in organic solvent (acetonitrile, methanol, etc.) first. 2.;;For very hydrophobic peptides, try dissolving the peptide in a small amount of DMSO, and then dilute the solution with water to the desired concentration.
Boc-Ile-Glu-Gly-Arg-AMC (IEGR-AMC) 是一种激活因子X (FXa) 特异性的荧光肽底物，用于因子 VIII 的测定。
Boc-Ile-Glu-Gly-Arg-AMC Chemical Structure
CAS No. : 65147-06-0
规格
是否有货
100 mg
;
询价
;
250 mg
;
询价
;
500 mg
;
询价
;
* Please select Quantity before adding items.
生物活性
Boc-Ile-Glu-Gly-Arg-AMC (IEGR-AMC) is an activated factor X (FXa) specific fluorogenic peptide substrate used for Factor VIII determination[1].
分子量
730.81
Formula
C34H50N8O10
CAS 号
65147-06-0
Sequence
Boc-Ile-Glu-Gly-Arg-AMC
Sequence Shortening
Boc-IEGR-AMC
运输条件
Room temperature in continental US; may vary elsewhere.
储存方式
Please store the product under the recommended conditions in the Certificate of Analysis.
参考文献
[1]. Eich S, et al. Factor VIII determination in patient’s plasma and concentrates: a novel test equally suited for both matrices. Blood Coagul Fibrinolysis. 2003 Jun;14(4):347-53.
Ac-Ile-Glu-Thr-Asp-pNA is a substrate for caspase-8. Caspase-8 binds to and cleaves the Ile-Glu-Thr-Asp (IETD) peptide sequence to release p-nitroalinide, which can be quantified by colorimetric detection at 405 nm as a measure of enzyme activity[1].
分子量
638.62
Formula
C27H38N6O12
CAS 号
219138-21-3
Sequence Shortening
Ac-IETD-pNA
运输条件
Room temperature in continental US; may vary elsewhere.
储存方式
Please store the product under the recommended conditions in the Certificate of Analysis.
参考文献
[1]. Smith GK, et al. Expression, preparation, and high-throughput screening of caspase-8: discovery of redox-based and steroid diacid inhibition. Arch Biochem Biophys. 2002;399(2):195-205.
H-Ile-Pro-Pro-OH, a milk-derived peptide[1], inhibits angiotensin-converting enzyme (ACE)[1] with an IC50 of 5 μM[2]. Antihypertensive tripeptides[1].
IC50 Target
IC50: 5 μM (ACE)[2]
体外研究 (In Vitro)
Ile-Pro-Pro (IPP; 1 nM, 0.1 μM, and 10 μM) increases nitric oxide (NO) production in human umbilical vein endothelial cells (HUVECs)[1].
MCE has not independently confirmed the accuracy of these methods. They are for reference only.
体内研究 (In Vivo)
Ile-Pro-Pro (IPP; 30 mg/kg per day; 0.3 g/L) attenuates arterial dysfunction in L-NAME-treated rats[1] Ile-Pro-Pro decreases blood pressure in spontaneously hypertensive rats (SHR) [1]. Ile-Pro-Pro attenuates the development of atherosclerosis in apolipoprotein E-deficient (apoE(-/-)) mice[1].
MCE has not independently confirmed the accuracy of these methods. They are for reference only.
分子量
325.40
Formula
C16H27N3O4
CAS 号
26001-32-1
运输条件
Room temperature in continental US; may vary elsewhere.
储存方式
Please store the product under the recommended conditions in the Certificate of Analysis.
参考文献
[1]. Nonaka A, et al. The milk-derived peptides Val-Pro-Pro and Ile-Pro-Pro attenuate arterial dysfunction in L-NAME-treated rats. Hypertens Res. 2014 Aug;37(8):703-7.
[2]. Nakamura Y, et al. Purification and characterization of angiotensin I-converting enzyme inhibitors from sour milk. J Dairy Sci. 1995 Apr;78(4):777-83.
H-Ile-Pro-Pro-OH hydrochloride, a milk-derived peptide[1], inhibits angiotensin-converting enzyme (ACE)[1] with an IC50 of 5 μM[2]. Antihypertensive tripeptides[1].
IC50 Target
IC50: 5 μM (ACE)[2]
体外研究 (In Vitro)
Ile-Pro-Pro (IPP; 1 nM, 0.1 μM, and 10 μM) increases nitric oxide (NO) production in human umbilical vein endothelial cells (HUVECs)[1].
MCE has not independently confirmed the accuracy of these methods. They are for reference only.
体内研究 (In Vivo)
Ile-Pro-Pro (IPP; 30 mg/kg per day; 0.3 g/L) attenuates arterial dysfunction in L-NAME-treated rats[1] Ile-Pro-Pro decreases blood pressure in spontaneously hypertensive rats (SHR) [1]. Ile-Pro-Pro attenuates the development of atherosclerosis in apolipoprotein E-deficient (apoE(-/-)) mice[1].
MCE has not independently confirmed the accuracy of these methods. They are for reference only.
分子量
361.86
Formula
C16H28ClN3O4
CAS 号
1208862-61-6
运输条件
Room temperature in continental US; may vary elsewhere.
储存方式
Sealed storage, away from moisture and light
Powder
-80deg;C
2 years
-20deg;C
1 year
*In solvent : -80deg;C, 6 months; -20deg;C, 1 month (sealed storage, away from moisture and light)
溶解性数据
In Vitro:;
H2O : 55 mg/mL (151.99 mM; Need ultrasonic)
DMSO : 36.67 mg/mL (101.34 mM; Need ultrasonic)
配制储备液
浓度溶剂体积质量
1 mg
5 mg
10 mg
1 mM
2.7635 mL
13.8175 mL
27.6350 mL
5 mM
0.5527 mL
2.7635 mL
5.5270 mL
10 mM
0.2763 mL
1.3817 mL
2.7635 mL
*
请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液；一旦配成溶液，请分装保存，避免反复冻融造成的产品失效。 储备液的保存方式和期限：-80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light)。-80°C 储存时，请在 6 个月内使用，-20°C 储存时，请在 1 个月内使用。
In Vivo:
请根据您的实验动物和给药方式选择适当的溶解方案。以下溶解方案都请先按照 In Vitro 方式配制澄清的储备液，再依次添加助溶剂：
[1]. Nonaka A, et al. The milk-derived peptides Val-Pro-Pro and Ile-Pro-Pro attenuate arterial dysfunction in L-NAME-treated rats. Hypertens Res. 2014 Aug;37(8):703-7.
[2]. Nakamura Y, et al. Purification and characterization of angiotensin I-converting enzyme inhibitors from sour milk. J Dairy Sci. 1995 Apr;78(4):777-83.
Diprotin A TFA;(Synonyms: Ile-Pro-Ile TFA) 纯度: 98.80%
Diprotin A TFA (Ile-Pro-Ile TFA) 是二肽基肽酶 IV (DPP-IV) 的抑制剂。
Diprotin A TFA Chemical Structure
CAS No. : 209248-71-5
规格
价格
是否有货
数量
10;mM;*;1 mL in Water
￥715
In-stock
5 mg
￥650
In-stock
10 mg
￥990
In-stock
25 mg
￥2100
In-stock
50 mg
￥2900
In-stock
100 mg
;
询价
;
200 mg
;
询价
;
* Please select Quantity before adding items.
Diprotin A TFA 相关产品
bull;相关化合物库:
Bioactive Compound Library Plus
Metabolism/Protease Compound Library
Peptidomimetic Library
Diabetes Related Compound Library
Peptide Library
生物活性
Diprotin A TFA (Ile-Pro-Ile TFA) is an inhibitor of dipeptidyl peptidase IV (DPP-IV)[1].
IC50 Target
DPP-IV[1].
体外研究 (In Vitro)
Diprotin A increases the phosphorylation of Src and vascular endothelial-cadherin (VE-cadherin) in human endothelial cells and disrupts endothelial cell-to-cell junctions, which are attenuated by CXCR4 (receptor of SDF-1α)-blocker or Src-inhibitor[1].
MCE has not independently confirmed the accuracy of these methods. They are for reference only.
体内研究 (In Vivo)
In the model of retinopathy of prematurity, Diprotin A increases not only retinal vascularity but also leakage. Additionally, in the murine diabetic retinopathy model, Diprotin A increases the phosphorylation of Src and VE-cadherin and aggravates vascular leakage in the retinas. Collectively, Diprotin A induces vascular leakage by augmenting the SDF-1α/CXCR4/Src/VE-cadherin signaling pathway[1].
MCE has not independently confirmed the accuracy of these methods. They are for reference only.
分子量
455.47
Formula
C19H32F3N3O6
CAS 号
209248-71-5
运输条件
Room temperature in continental US; may vary elsewhere.
