Zinquin ethyl ester is a zinc ion-specific fluorophore. Zinquin ethyl ester is a lipophilic, zinc-sensitive, fluorescent derivative of Zinquin that is able to penetrate cell membranes[1].
分子量
414.47
Formula
C21H22N2O5S
CAS 号
181530-09-6
运输条件
Room temperature in continental US; may vary elsewhere.
储存方式
Please store the product under the recommended conditions in the Certificate of Analysis.
参考文献
[1]. Kevyn E Merten, et al. Zinc inhibits doxorubicin-activated calcineurin signal transduction pathway in H9c2 embryonic rat cardiac cells. Exp Biol Med (Maywood). 2007 May;232(5):682-9.
Cy5-SE (Cy5 NHS Ester) is a reactive dye for the labeling of amino-groups in peptides, proteins, and oligonucleotides. This dye requires small amount of organic co-solvent (such as DMF or DMSO) to be used in labeling reaction. This reagent is ideal for very cost-efficient labeling of soluble proteins, as well as all kinds of peptides and oligonucleotides. This reagent also works well in organic solvents for small molecule labeling. Excitation (nm):649, Emission (nm): 670.
体外研究 (In Vitro)
Guidelines (Following is our recommended protocol. This protocol only provides a guideline, and should be modified according to your specific needs). Conjugation of SELP Analogues with Cy5-SE. 1. 1 mL of each 100 μg/mL SELP solution in 100 mM sodium bicarbonate buffer (pH 8.3) is mixed with 10 μL of 1.2 mg/mL Cy5 mono NHS-ester in 10% DMSO and incubates for 2 hours on ice. 2. To quench the reaction, 50 μL of 1 M Tris-HCl (pH 8.0) is added to the reaction solution. 3. Reaction mixtures are loaded onto 1.5 mL Sephadex G-25 columns, and Cy5-conjugated SELPs are eluted by centrifugation for 3 minutes at 1050g[1].
MCE has not independently confirmed the accuracy of these methods. They are for reference only.
分子量
753.88
Formula
C37H43N3O10S2
CAS 号
146368-14-1
运输条件
Room temperature in continental US; may vary elsewhere.
储存方式
-20deg;C, sealed storage, away from moisture and light
*In solvent : -80deg;C, 6 months; -20deg;C, 1 month (sealed storage, away from moisture and light)
溶解性数据
In Vitro:;
DMSO : 25 mg/mL (33.16 mM; Need ultrasonic)
配制储备液
浓度溶剂体积质量
1 mg
5 mg
10 mg
1 mM
1.3265 mL
6.6324 mL
13.2647 mL
5 mM
0.2653 mL
1.3265 mL
2.6529 mL
10 mM
0.1326 mL
0.6632 mL
1.3265 mL
*
请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液;一旦配成溶液,请分装保存,避免反复冻融造成的产品失效。 储备液的保存方式和期限:-80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light)。-80°C 储存时,请在 6 个月内使用,-20°C 储存时,请在 1 个月内使用。
In Vivo:
请根据您的实验动物和给药方式选择适当的溶解方案。以下溶解方案都请先按照 In Vitro 方式配制澄清的储备液,再依次添加助溶剂:
将 2 g 磺丁基醚 β-环糊精加入 5 mL 生理盐水中,再用生理盐水定容至 10 mL,完全溶解,澄清透明
*以上所有助溶剂都可在 MCE 网站选购。
参考文献
[1]. Jeon HY, et al. Array-Based High-Throughput Analysis of Silk-Elastinlike Protein Polymer Degradation and C-Peptide Release by Proteases. Anal Chem. 2016;88(10):5398-5405.
Calcein tetraethyl ester is a fluorescent dye, is used in biology as it can be transported through the cellular membrane into live cells, which makes it useful for testing of cell viability and for short-term labeling of cells.
分子量
734.75
Formula
C38H42N2O13
CAS 号
1170856-93-5
中文名称
钙黄绿素四乙酯;钙黄绿素四乙基酯
运输条件
Room temperature in continental US; may vary elsewhere.
Calcein tetraethyl ester is a fluorescent dye, is used in biology as it can be transported through the cellular membrane into live cells, which makes it useful for testing of cell viability and for short-term labeling of cells.
分子量
734.75
Formula
C38H42N2O13
CAS 号
1170856-93-5
中文名称
钙黄绿素四乙酯;钙黄绿素四乙基酯
运输条件
Room temperature in continental US; may vary elsewhere.
Cyanine5 NHS ester chloride is a red emitting fluorescent dye for labeling of amino-groups in peptides, proteins, and oligonucleotides[1].
体外研究 (In Vitro)
Cyanine-5 NHS ester amine-reactive red emitting fluorescent dye is used to react with the free amino group on the cysteine amino acid present in terminal peptides used for end-capping Poly(β-amino esters) (pBAEs) backbones[1].
MCE has not independently confirmed the accuracy of these methods. They are for reference only.
分子量
616.19
Formula
C36H42ClN3O4
CAS 号
1032678-42-4
运输条件
Room temperature in continental US; may vary elsewhere.
储存方式
Please store the product under the recommended conditions in the Certificate of Analysis.
溶解性数据
In Vitro:;
DMSO : 250 mg/mL (405.72 mM; Need ultrasonic)
配制储备液
浓度溶剂体积质量
1 mg
5 mg
10 mg
1 mM
1.6229 mL
8.1144 mL
16.2288 mL
5 mM
0.3246 mL
1.6229 mL
3.2458 mL
10 mM
0.1623 mL
0.8114 mL
1.6229 mL
In Vivo:
请根据您的实验动物和给药方式选择适当的溶解方案。以下溶解方案都请先按照 In Vitro 方式配制澄清的储备液,再依次添加助溶剂:
5-TAMRA-SE is an amine-reactive fluorescent agent, its conjugates yield bright, pH-insensitive orange-red fluorescence (approximate excitation/emission maxima ~546/579) with good photostability.
分子量
527.52
Formula
C29H25N3O7
CAS 号
150810-68-7
中文名称
5-羧基四甲基罗丹明琥珀酰亚胺酯
运输条件
Room temperature in continental US; may vary elsewhere.
储存方式
4deg;C, protect from light, stored under nitrogen
*In solvent : -80deg;C, 6 months; -20deg;C, 1 month (protect from light, stored under nitrogen)
溶解性数据
In Vitro:;
DMF : 10 mg/mL (18.96 mM; Need ultrasonic)
DMSO : 5 mg/mL (9.48 mM; Need ultrasonic and warming)
配制储备液
浓度溶剂体积质量
1 mg
5 mg
10 mg
1 mM
1.8957 mL
9.4783 mL
18.9566 mL
5 mM
0.3791 mL
1.8957 mL
3.7913 mL
10 mM
0.1896 mL
0.9478 mL
1.8957 mL
*
请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液;一旦配成溶液,请分装保存,避免反复冻融造成的产品失效。 储备液的保存方式和期限:-80°C, 6 months; -20°C, 1 month (protect from light, stored under nitrogen)。-80°C 储存时,请在 6 个月内使用,-20°C 储存时,请在 1 个月内使用。
In Vivo:
请根据您的实验动物和给药方式选择适当的溶解方案。以下溶解方案都请先按照 In Vitro 方式配制澄清的储备液,再依次添加助溶剂:
请依序添加每种溶剂:;10% DMF ;; 90% (20% SBE-β-CD in saline)
Solubility: ≥ 1 mg/mL (1.90 mM); Clear solution
*以上所有助溶剂都可在 MCE 网站选购。
参考文献
[1]. Kierzek R, et al.5′-Amino Pyrene Provides a Sensitive, Nonperturbing Fluorescent Probe of RNA Secondary and Tertiary Structure Formation. J Am Chem Soc (1993) 115:4985-4985
[2]. Schmidt T, et al. Local Stoichiometries Determined by Counting Individual Molecules. Anal Chem (1996) 68:4397-4397
Scutellarin methyl ester is a constituent of Breviscapine which is a crude extract of several flavonoids of Erigeron breviscapus[1][2].
分子量
476.39
Formula
C22H20O12
CAS 号
119262-68-9
中文名称
灯盏花乙素甲酯; 野黄芩苷甲酯
运输条件
Room temperature in continental US; may vary elsewhere.
储存方式
Please store the product under the recommended conditions in the Certificate of Analysis.
参考文献
[1]. Zhao M, et al. Structural identification of related substances in Breviscapine by UPLC-QTOF-MS. Zhongguo Zhong Yao Za Zhi. 2018 Jul;43(14):2872-2877.
[2]. Gao J, et al. Therapeutic Effects of Breviscapine in Cardiovascular Diseases: A Review. Front Pharmacol. 2017 May 23;8:289.