Eledoisin Related Peptide;(Synonyms: Eledoisin-Related Peptide; Eledoisin RP)
Eledoisin Related Peptide是物质P的类似物,刺激神经元并触发行为反应。它也是 tachykinin receptor 的配体。
Eledoisin Related Peptide Chemical Structure
CAS No. : 2990-43-4
规格
价格
是否有货
1 mg
¥770
询问价格 货期
5 mg
¥3100
询问价格 货期
10 mg
¥4900
询问价格 货期
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生物活性
Eledoisin Related Peptide is a Substance P analog that excites neurons and triggers behavioral responses. Eledoisin Related Peptide is also a tachykinin receptor ligand.
IC50 Target
Tachykinin receptor[1]
体内研究 (In Vivo)
Eledoisin Related Peptide shares with Substance P (SP) a common N-terminal amino acid sequence and has been shown by to have SP-like activity in the periphery (gut and salivary glands) and the CNS. Eledoisin-related peptide seems to be roughly equipotent with Substance P at identical ejection currents on the single-cell activity of neurons in this nucleus[2]. Both glutamate and substance P (and its analogue, eledoisin-related peptide) have excitatory effects on the activity of respiratory neurons and reflex interneurons[3].
MCE has not independently confirmed the accuracy of these methods. They are for reference only.
分子量
706.94
Formula
C34H58N8O6S
CAS 号
2990-43-4
Sequence
Lys-Phe-Ile-Gly-Leu-Met-NH2
Sequence Shortening
KFIGLM-NH2
运输条件
Room temperature in continental US; may vary elsewhere.
储存方式
Please store the product under the recommended conditions in the Certificate of Analysis.
Solvent Solubility
In Vitro:;
H2O
Peptide Solubility and Storage Guidelines:
1.;;Calculate the length of the peptide.
2.;;Calculate the overall charge of the entire peptide according to the following table:
;
Contents
Assign value
Acidic amino acid
Asp (D), Glu (E), and the C-terminal -COOH.
-1
Basic amino acid
Arg (R), Lys (K), His (H), and the N-terminal -NH2
+1
Neutral amino acid
Gly (G), Ala (A), Leu (L), Ile (I), Val (V), Cys (C), Met (M), Thr (T), Ser (S), Phe (F), Tyr (Y), Trp (W), Pro (P), Asn (N), Gln (Q)
0
3.;;Recommended solution:
Overall charge of peptide
Details
Negative (lt;0)
1.;;Try to dissolve the peptide in water first. 2.;;If water fails, add NH4OH (lt;50 μL). 3.;;If the peptide still does not dissolve, add DMSO (50-100 μL) to solubilize the peptide.
Positive (gt;0)
1.;;Try to dissolve the peptide in water first. 2.;;If water fails, try dissolving the peptide in a 10%-30% acetic acid solution. 3.;;If the peptide still does not dissolve, try dissolving the peptide in a small amount of DMSO.
Zero (=0)
1.;;Try to dissolve the peptide in organic solvent (acetonitrile, methanol, etc.) first. 2.;;For very hydrophobic peptides, try dissolving the peptide in a small amount of DMSO, and then dilute the solution with water to the desired concentration.
参考文献
[1]. Severini C, et al. The tachykinin peptide family. Pharmacol Rev. 2002 Jun;54(2):285-322.
[2]. Guyenet PG, et al. Excitation of neurons in the nucleus locus coeruleus by substance P and related peptides. Brain Res. 1977 Nov 4;136(1):178-84.
[3]. Henry JL, et al. Effects of glutamate, substance P and eledoisin-related peptide on solitary tract neurones involved in respiration and respiratory reflexes. Neuroscience. 1985 Mar;14(3):863-73.
Animal Administration [2]
Rats[2]
Twenty-two male albino rats (230-260 g) are anesthetized with chloral hydrate (400 mg/kg, i.p.) and prepared for recording. Briefly, a singlebarrel recording pipette (tip 1 μm) is glued alongside a conventional five-barrel micropipette (tip 15-25/μm) then filled with 2 M NaCI saturated with Fast Green (impedance 4-7 M). The distance between the tip of the recording electrode and that of the five-barrel micropipette is 15-25/zm. Fast Green is ejected at the end of the experiment to identify the recording site. One side barrel of the five-barrel micropipette is loaded with 4 M NaCI for automatic current balancing and the others with three of the following solutions: L-epinephrine bitartrate (0. l M, pH 4.0), L-norepinephrine bitartrate (0.1 M, pH 4.0), Substance P (2.75 mM), physalaemin (2.6 mM), substance P 4-11 octapeptide (3.1 mM), eledoisin-related peptide (20 mM), neurotensin, bradykinin triacetate (15 mM), met-enkephalin (6.5 mM), TRH (48 mM). Spontaneously active cells are recorded in the locus coeruleus or in the nearby mesencephalic nucleus of the fifth nerve whose cells are easily identified by their increased activity upon manipulation of the jaw[2].
MCE has not independently confirmed the accuracy of these methods. They are for reference only.
参考文献
[1]. Severini C, et al. The tachykinin peptide family. Pharmacol Rev. 2002 Jun;54(2):285-322.
[2]. Guyenet PG, et al. Excitation of neurons in the nucleus locus coeruleus by substance P and related peptides. Brain Res. 1977 Nov 4;136(1):178-84.
[3]. Henry JL, et al. Effects of glutamate, substance P and eledoisin-related peptide on solitary tract neurones involved in respiration and respiratory reflexes. Neuroscience. 1985 Mar;14(3):863-73.
Eledoisin (Eledone peptide) is a specific agonist of NK2 and NK3 receptors.
IC50 Target
NK2 and NK3 receptors[1].
体外研究 (In Vitro)
Eledoisin (Eledone peptide) increases the value recorded under basal conditions by 24.5±3.7%; this stimulation is significantly (P<0.01) lowered to 13.1±1.9% by the simultaneous presence of CP99994. The same protocol is also used to characterize the sensitivity of Eledoisin stimulation to 0.1 μM SR48968 or 0.1 μM SB222200. SR48968 significantly (P < 0.01) lower the stimulation by Eledoisin, while SB222200 has no effect. Eledoisin stimulation is reduced by CP99994 and SR48968, NK1 and NK2 antagonists, respectively[1].
MCE has not independently confirmed the accuracy of these methods. They are for reference only.
体内研究 (In Vivo)
Eledoisin (Eledone peptide; 0.1-1 nmol/kg) injected into rats produces a biphasic cardiovascular response that consists of an initial fall of systemic blood pressure (8-15 mm Hg) followed by a rise (20-22 mm Hg). Intracerebroventricular injection of Eledoisin produces an enhancement of grooming and scratching behavior in mice[2].
MCE has not independently confirmed the accuracy of these methods. They are for reference only.
分子量
1188.40
Formula
C54H85N13O15S
CAS 号
69-25-0
Sequence
{Glp}-Pro-Ser-Lys-Asp-Ala-Phe-Ile-Gly-Leu-Met-NH2
Sequence Shortening
{Glp}-PSKDAFIGLM-NH2
中文名称
章鱼唾腺精
运输条件
Room temperature in continental US; may vary elsewhere.
储存方式
Powder
-80deg;C
2 years
-20deg;C
1 year
In solvent
-80deg;C
6 months
-20deg;C
1 month
溶解性数据
In Vitro:;
H2O : 18.15 mg/mL (15.27 mM; Need ultrasonic and warming)