DAPI dihydrochloride (4′,6-diamidino-2-phenylindole) is a fluorescent stain by binding in the minor grove of A-T rich sequences of DNA.
体外研究 (In Vitro)
DAPI (4′,6-diamidino-2-phenylindole) is a DNA-specific probe which forms a fluorescent complex by attaching in the minor grove of A-T rich sequences of DNA. It also forms nonfluorescent intercalative complexes with double-stranded nucleic acids. DAPI can be used as a DNA-specific probe for flow cytometry, chromosome staining, DNA visualization and quantitation in histochemistry and biochemistry[1].
MCE has not independently confirmed the accuracy of these methods. They are for reference only.
分子量
350.25
Formula
C16H17Cl2N5
CAS 号
28718-90-3
中文名称
4′,6-二脒基-2-苯基吲哚
运输条件
Room temperature in continental US; may vary elsewhere.
储存方式
-20deg;C, sealed storage, away from moisture and light
*In solvent : -80deg;C, 6 months; -20deg;C, 1 month (sealed storage, away from moisture and light)
溶解性数据
In Vitro:;
DMSO : 75 mg/mL (214.13 mM; Need ultrasonic)
H2O : 5.56 mg/mL (15.87 mM; ultrasonic and warming and heat to 60°C)
配制储备液
浓度溶剂体积质量
1 mg
5 mg
10 mg
1 mM
2.8551 mL
14.2755 mL
28.5510 mL
5 mM
0.5710 mL
2.8551 mL
5.7102 mL
10 mM
0.2855 mL
1.4276 mL
2.8551 mL
*
请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液;一旦配成溶液,请分装保存,避免反复冻融造成的产品失效。 储备液的保存方式和期限:-80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light)。-80°C 储存时,请在 6 个月内使用,-20°C 储存时,请在 1 个月内使用。
In Vivo:
请根据您的实验动物和给药方式选择适当的溶解方案。以下溶解方案都请先按照 In Vitro 方式配制澄清的储备液,再依次添加助溶剂:
荧光染料Dye Reagents TMB dihydrochloride;(Synonyms: BM blue dihydrochloride; Sure Blue TMB dihydrochloride) 纯度: 99.29%
TMB dihydrochloride (BM blue dihydrochloride) 是免疫组化和ELISA的显色底物。
TMB dihydrochloride Chemical Structure
CAS No. : 64285-73-0
规格
价格
是否有货
数量
Free Sample (0.1-0.5 mg)
;
Apply now
;
10;mM;*;1 mL in DMSO
¥500
In-stock
500 mg
¥400
In-stock
1 g
¥500
In-stock
5 g
¥1100
In-stock
10 g
;
询价
;
50 g
;
询价
;
* Please select Quantity before adding items.
TMB dihydrochloride 相关产品
bull;相关化合物库:
Bioactive Compound Library Plus
生物活性
TMB dihydrochloride (BM blue dihydrochloride) is a chromogenic substrate used in staining procedures in immunohistochemistry as well as being a visualising reagent used in enzyme-linked immunosorbent assays (ELISA).
分子量
313.27
Formula
C16H22Cl2N2
CAS 号
64285-73-0
运输条件
Room temperature in continental US; may vary elsewhere.
储存方式
4deg;C, sealed storage, away from moisture and light
*In solvent : -80deg;C, 6 months; -20deg;C, 1 month (sealed storage, away from moisture and light)
溶解性数据
In Vitro:;
DMSO : ≥ 33 mg/mL (105.34 mM)
H2O : 11.11 mg/mL (35.46 mM; Need ultrasonic)
*“≥” means soluble, but saturation unknown.
配制储备液
浓度溶剂体积质量
1 mg
5 mg
10 mg
1 mM
3.1921 mL
15.9607 mL
31.9213 mL
5 mM
0.6384 mL
3.1921 mL
6.3843 mL
10 mM
0.3192 mL
1.5961 mL
3.1921 mL
*
请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液;一旦配成溶液,请分装保存,避免反复冻融造成的产品失效。 储备液的保存方式和期限:-80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light)。-80°C 储存时,请在 6 个月内使用,-20°C 储存时,请在 1 个月内使用。
荧光染料Dye Reagents TMB dihydrochloride;(Synonyms: BM blue dihydrochloride; Sure Blue TMB dihydrochloride) 纯度: 99.29%
TMB dihydrochloride (BM blue dihydrochloride) 是免疫组化和ELISA的显色底物。
TMB dihydrochloride Chemical Structure
CAS No. : 64285-73-0
规格
价格
是否有货
数量
Free Sample (0.1-0.5 mg)
;
Apply now
;
10;mM;*;1 mL in DMSO
¥500
In-stock
500 mg
¥400
In-stock
1 g
¥500
In-stock
5 g
¥1100
In-stock
10 g
;
询价
;
50 g
;
询价
;
* Please select Quantity before adding items.
TMB dihydrochloride 相关产品
bull;相关化合物库:
Bioactive Compound Library Plus
生物活性
TMB dihydrochloride (BM blue dihydrochloride) is a chromogenic substrate used in staining procedures in immunohistochemistry as well as being a visualising reagent used in enzyme-linked immunosorbent assays (ELISA).
分子量
313.27
Formula
C16H22Cl2N2
CAS 号
64285-73-0
运输条件
Room temperature in continental US; may vary elsewhere.
储存方式
4deg;C, sealed storage, away from moisture and light
*In solvent : -80deg;C, 6 months; -20deg;C, 1 month (sealed storage, away from moisture and light)
溶解性数据
In Vitro:;
DMSO : ≥ 33 mg/mL (105.34 mM)
H2O : 11.11 mg/mL (35.46 mM; Need ultrasonic)
*“≥” means soluble, but saturation unknown.
配制储备液
浓度溶剂体积质量
1 mg
5 mg
10 mg
1 mM
3.1921 mL
15.9607 mL
31.9213 mL
5 mM
0.6384 mL
3.1921 mL
6.3843 mL
10 mM
0.3192 mL
1.5961 mL
3.1921 mL
*
请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液;一旦配成溶液,请分装保存,避免反复冻融造成的产品失效。 储备液的保存方式和期限:-80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light)。-80°C 储存时,请在 6 个月内使用,-20°C 储存时,请在 1 个月内使用。
o-Dianisidine dihydrochloride is a colorimetric peroxidase substrate suitable for use in ELISA procedures. The compound has been used as a substrate to measure the oxidase activity of copper. o-Dianisidine dihydrochloride has also been used for a paper based-microfluidic device to semi-quantitatively measure lactate, uric acid, and glucose.
将 2 g 磺丁基醚 β-环糊精加入 5 mL 生理盐水中,再用生理盐水定容至 10 mL,完全溶解,澄清透明
*以上所有助溶剂都可在 MCE 网站选购。
参考文献
[1]. Iskra M et al. Copper and zinc concentrations and the activities of ceruloplasmin and superoxide dismutase inatherosclerosis obliterans. Biol Trace Elem Res. 2000 Jan;73(1):55-65.
[2]. Dungchai W et al. Use of multiple colorimetric indicators for paper-based microfluidic devices. Anal Chim Acta. 2010 Aug 3;674(2):227-33.