荧光染料Dye Reagents DAF-2DA;(Synonyms: 5,6-Diaminofluorescein diacetat)
DAF-2DA (5,6-Diaminofluorescein diacetat) 是测量 NO 最广泛使用的一种荧光探针。
DAF-2DA Chemical Structure
CAS No. : 205391-02-2
| 规格 | 是否有货 | ||
|---|---|---|---|
| 50 μg | 询价 |
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| 生物活性 |
DAF-2DA (5,6-Diaminofluorescein diacetat) is most widely probe for NO measurement. |
|---|---|
| 体外研究 (In Vitro) |
Nitric oxide is a free radical signal molecule. Various methods are available for measurement of NO. Out of all methods, fluorescent probes to localize NO is very widely used method. Diaminofl uorescein in diacetate form (DAF-2DA) is most widely probe for NO measurement. This method is based on application of 4,5-diaminofl uorescein diacetate (DAF-2DA) which is actively diffused into cells, once taken up by cells cytoplasmic esterases cleave the acetate groups to generate 4,5-diaminofl uorescein; DAF-2. The generated DAF-2 can readily react with N2O3, which is an oxidation product of NO to generate the highly fluorescent DAF-2T (triazolofl uorescein). MCE has not independently confirmed the accuracy of these methods. They are for reference only. |
| 分子量 |
446.41 |
| Formula |
C24H18N2O7 |
| CAS 号 |
205391-02-2 |
| 运输条件 |
Room temperature in continental US; may vary elsewhere. |
| 储存方式 |
Please store the product under the recommended conditions in the Certificate of Analysis. |
| 参考文献 |
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