标签归档：diacetate

Cetrorelix diacetate(Synonyms: SB-75 diacetate)

发表于由

Cetrorelix diacetate;(Synonyms: SB-75 diacetate)

Cetrorelix diacetate (SB-075 diacetate)是 GnRH 受体的强效拮抗剂，其 IC50 值为 1.21 nM。

Cetrorelix diacetateamp;;(Synonyms: SB-75 diacetate)

Cetrorelix diacetate Chemical Structure

CAS No. : 130143-01-0

规格 是否有货
100 mg ; 询价 ;
250 mg ; 询价 ;
500 mg ; 询价 ;

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Cetrorelix diacetate 的其他形式现货产品:

Cetrorelix Acetate

生物活性

Cetrorelix diacetate (SB-075 diacetate) is a potent gonadotropin-releasing hormone (GnRH) receptor antagonist with an IC50 of 1.21 nM[1].

IC50 Target

IC50: 1.21 nM GnRH[1].
体外研究
(In Vitro)

Cetrorelix diacetate inhibits growth of ES-2 cell line at 1000 ng/ml. Cetrorelix diacetate has comparable antiproliferative effects as GnRH-I agonists indicating that the dichotomy of GnRH-I agonists and antagonists might not apply to the GnRH-I system in cancer cells[2].

MCE has not independently confirmed the accuracy of these methods. They are for reference only.
Clinical Trial

分子量

1551.14

Formula

C74H100ClN17O18
CAS 号

130143-01-0
Sequence Shortening

Ac-{2-Naph-Ala}-{Cl-Phe}-{3Py-Ala}-SY-{Cit}-LRPA-NH2
中文名称

二醋酸西曲瑞克
运输条件

Room temperature in continental US; may vary elsewhere.
储存方式

Please store the product under the recommended conditions in the Certificate of Analysis.
参考文献

  • [1]. Beckers T et al. Characterization of gonadotropin-releasing hormone analogs based on a sensitive cellular luciferase reporter gene assay. Anal Biochem. 1997 Aug 15;251(1):17-23.

    [2]. Gründker C et al. Role of gonadotropin-releasing hormone (GnRH) in ovarian cancer. Reprod Biol Endocrinol. 2003 Oct 7;1:65.

Splenopentin diacetate(Synonyms: 醋酸脾脏五肽)

发表于由

Splenopentin diacetate;(Synonyms: 醋酸脾脏五肽)

Splenopentin diacetate 是一种合成的免疫调节性五肽，对应于脾脏激素脾脏蛋白的残基 32-36。Splenopentin diacetate 可影响早期的 T 细胞和 B 细胞分化，从而增加 γ 射线照射后小鼠体内抗体形成细胞的数量。

Splenopentin diacetateamp;;(Synonyms: 醋酸脾脏五肽)

Splenopentin diacetate Chemical Structure

CAS No. : 105184-37-0

规格 是否有货
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250 mg ; 询价 ;
500 mg ; 询价 ;

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生物活性

Splenopentin diacetate is a synthetic immunomodulating pentapeptide corresponding to the residues 32-36 of the splenic hormone splenin. Splenopentin diacetate influences both early T and B cell differentiation, to increase the number of antibody-forming cells in mice after gamma irradiation[1][2].

体外研究
(In Vitro)

Splenopentin (1-100 μM) augments human NK cells and has no cytotoxicity[2].
Splenopentin (0.1 ng/mL-10 μg/mL; 3 h) induces phenotypic differentiation of both T- and B-cell precursor cells[3].

MCE has not independently confirmed the accuracy of these methods. They are for reference only.
体内研究
(In Vivo)

Splenopentin (1 mg/kg; i.p. three times a week for 7-90 d) accelerates Langerhans cell recruitment and leads to pretreatment levels of Langerhans cell (LC) density in the skin of mice[4].
Splenopentin (0.01-100 μg per mouse; i.p.) has no demonstrable effect on neuromuscular transmission of mice[3].

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model: Female Balb/c/Bln mice were injected Cyclophosphamide or Dexamethasone for 5 consecutive days[4]
Dosage: 1 mg/kg
Administration: I.p. three times a week for 7-90 days
Result: Reached the normal number of epidermal LCs at 28 days after the beginning of Cyclophosphamide treatment.
Accelerated restoration process and reached the normal LC density in the skin after 70 days in Dexamethasone treatment grops.

分子量

813.90

Formula

C35H59N9O13
CAS 号

105184-37-0
中文名称

醋酸脾脏五肽
运输条件

Room temperature in continental US; may vary elsewhere.
储存方式

Please store the product under the recommended conditions in the Certificate of Analysis.
Solvent Solubility
In Vitro:;

H2O

Peptide Solubility and Storage Guidelines:

1.;;Calculate the length of the peptide.

2.;;Calculate the overall charge of the entire peptide according to the following table:

; Contents Assign value
Acidic amino acid Asp (D), Glu (E), and the C-terminal -COOH. -1
Basic amino acid Arg (R), Lys (K), His (H), and the N-terminal -NH2 +1
Neutral amino acid Gly (G), Ala (A), Leu (L), Ile (I), Val (V), Cys (C), Met (M), Thr (T), Ser (S), Phe (F), Tyr (Y), Trp (W), Pro (P), Asn (N), Gln (Q) 0

3.;;Recommended solution:

Overall charge of peptide Details
Negative (lt;0) 1.;;Try to dissolve the peptide in water first.
2.;;If water fails, add NH4OH (lt;50 μL).
3.;;If the peptide still does not dissolve, add DMSO (50-100 μL) to solubilize the peptide.
Positive (gt;0) 1.;;Try to dissolve the peptide in water first.
2.;;If water fails, try dissolving the peptide in a 10%-30% acetic acid solution.
3.;;If the peptide still does not dissolve, try dissolving the peptide in a small amount of DMSO.
Zero (=0) 1.;;Try to dissolve the peptide in organic solvent (acetonitrile, methanol, etc.) first.
2.;;For very hydrophobic peptides, try dissolving the peptide in a small amount of DMSO, and then dilute the solution with water to the desired concentration.
参考文献

  • [1]. Singh VK, et, al. Thymopentin and splenopentin as immunomodulators. Current status. Immunol Res. 1998;17(3):345-68.

    [2]. Rastogi A, et, al. Augmentation of human natural killer cells by splenopentin analogs. FEBS Lett. 1993 Feb 8;317(1-2):93-5.

    [3]. Audhya T, et, al. Contrasting biological activities of thymopoietin and splenin, two closely related polypeptide products of thymus and spleen. Proc Natl Acad Sci U S A. 1984 May;81(9):2847-9.

    [4]. Gruner S, et, al. Stimulation of the recruitment of epidermal Langerhans cells by splenopentin. Arch Dermatol Res. 1990;281(8):526-9.

Tuftsin diacetate

发表于由

Tuftsin diacetate;

Tuftsin diacetate 是一种四肽，是巨噬细胞/小胶质细胞 (macrophage/microglial) 的激活剂。

Tuftsin diacetateamp;;

Tuftsin diacetate Chemical Structure

CAS No. : 72103-53-8

规格 是否有货
100 mg ; 询价 ;
250 mg ; 询价 ;
500 mg ; 询价 ;

* Please select Quantity before adding items.

Tuftsin diacetate 的其他形式现货产品:

Tuftsin

生物活性

Tuftsin diacetate, a tetrapeptide, is a macrophage/microglial activator.

体外研究
(In Vitro)

Tuftsin is a tetrapeptide, Thr-Lys-Pro-Arg, which resides in the Fc-domain of the heavy chain of immunoglobulin G. Tuftsin possesses a broad spectrum of activities related primarily to the immune system function and exerts on phagocytic cells, notably on macrophages. Tuftsin’s capacity to augment cellular activation is mediated by specific receptors that are identified, characterized, and recently isolated from rabbit peritoneal granulocytes[1]. Tuftsin, a macrophage/microglial activator, dramatically improves the clinical course of experimental autoimmune encephalomyelitis (EAE), a well-established animal model for MS. Tuftsin administration correlates with upregulation of the immunosuppressive Helper-2 Tcell (Th2) cytokine transcription factor GATA-3. Tuftsin promotes phagocytic activity for cells of monocytic origin, such as neutrophils, macrophages and microglia, all of which are thought to express Tuftsin receptors[2].

MCE has not independently confirmed the accuracy of these methods. They are for reference only.
分子量

620.70

Formula

C25H48N8O10
CAS 号

72103-53-8
Sequence

Thr-Lys-Pro-Arg
Sequence Shortening

TKPR
运输条件

Room temperature in continental US; may vary elsewhere.
储存方式

Please store the product under the recommended conditions in the Certificate of Analysis.
Solvent Solubility
In Vitro:;

H2O

Peptide Solubility and Storage Guidelines:

1.;;Calculate the length of the peptide.

2.;;Calculate the overall charge of the entire peptide according to the following table:

; Contents Assign value
Acidic amino acid Asp (D), Glu (E), and the C-terminal -COOH. -1
Basic amino acid Arg (R), Lys (K), His (H), and the N-terminal -NH2 +1
Neutral amino acid Gly (G), Ala (A), Leu (L), Ile (I), Val (V), Cys (C), Met (M), Thr (T), Ser (S), Phe (F), Tyr (Y), Trp (W), Pro (P), Asn (N), Gln (Q) 0

3.;;Recommended solution:

Overall charge of peptide Details
Negative (lt;0) 1.;;Try to dissolve the peptide in water first.
2.;;If water fails, add NH4OH (lt;50 μL).
3.;;If the peptide still does not dissolve, add DMSO (50-100 μL) to solubilize the peptide.
Positive (gt;0) 1.;;Try to dissolve the peptide in water first.
2.;;If water fails, try dissolving the peptide in a 10%-30% acetic acid solution.
3.;;If the peptide still does not dissolve, try dissolving the peptide in a small amount of DMSO.
Zero (=0) 1.;;Try to dissolve the peptide in organic solvent (acetonitrile, methanol, etc.) first.
2.;;For very hydrophobic peptides, try dissolving the peptide in a small amount of DMSO, and then dilute the solution with water to the desired concentration.
参考文献

  • [1]. Fridkin M, et al. Tuftsin: its chemistry, biology, and clinical potential. Crit Rev Biochem Mol Biol. 1989;24(1):1-40.

    [2]. Wu M, et al. Tuftsin promotes an anti-inflammatory switch and attenuates symptoms in experimentalautoimmune encephalomyelitis. PLoS One. 2012;7(4):e34933.

日本同仁化学FDA试剂货号：F209 Fluorescein diacetate| DOJINDO

发表于由

上海金畔生物科技有限公司代理日本同仁化学 DOJINDO代理商全线产品，欢迎访问官网了解更多信息

FDA试剂货号：F209
Fluorescein diacetate
Fluorescein diacetate
商品信息
储存条件：0-5度保存，避光
运输条件：室温
分子式：

C24H16O7

分子量：

416.38

特点：

 

● 激发和发射波长分别为488 nm和530 nm

● 可用于流式细胞仪

SDS下载

选择规格：
1mg

期货

细胞染色

FDA试剂货号：F209 Fluorescein diacetate

FDA试剂货号：F209 Fluorescein diacetate

规格性状
产品概述
荧光特性
参考文献
常见问题Q&A

规格性状

规格

特性：该产物为白色结晶粉末或固体，可溶于二甲基亚砜。

可溶于二甲基亚砜：试验成功

NMR光谱：试验成功

产品概述

FDA可透过细胞膜并作为荧光素积蓄在活细胞内。由于荧光素较BCECF或Calcein的亲水性低，因此荧光素从细胞中渗漏的量也高。FDA也可用于流式细胞仪。荧光素的激发和发射波长分别为488 nm和530 nm。

荧光特性

荧光特性：λex=488 nm, λem=530 nm

参考文献

1) B. Rotman and B. W. Papermaster, “Membrane Properties of Living Mammalian Cells as Studied by Enzymatic Hydrolysis of Fluorogenic Esters”, Proc. Natl. Acad. Sci. USA, 1966, 55, 134.

2) H. R. Hulett, W. A. Bonner, J. Barrett and L. A. Herzenberg, “Cell Sorting: Automated Separation of Mammalian Cells as a Function of Intercellular Fluorescence”, Science, 1969, 166, 747.

3) K. H. Jones and J. A. Senft, “An Improved Method to Determine Cell Viability by Simultaneous Staining with Fluorescein Diacetate-Propidium Iodide”,J.Histochem.Cytochem., 1985, 33, 77.

4) K. McGinnes, G. Chapman, R. Marks and R. Penny, “A Fluorescence NK Assay Using Flow Cytometry”, J. Immunol. Methods, 1986, 86, 7.

5) W. M. J. Vuist, F. V. Buitenen, M. A. De Rie, A. Hekman, P. Ruemke and C. J. M. Melief, “Potentiation by Interleukin 2 of Burkitt’s Lymphoma Therapy with Anti-Pan B (Anti-CD19) Monoclonal Antibodies in a Mouse Xenotransplantation Model”, Cancer Res., 1989, 49, 3783.

6) E. Prosperi, “Intracellular Turnover of Fluorescein Diacetate. Influence of Membrane Ionic Gradients on Fluorescein Efflux”, Histochem.J., 1990, 22, 227.

常见问题Q&A

Q1:如何使用FDA。

 
 

 

 

 

A1:下面给出一个例子。请根据细胞的种类，观察条件讨论细胞的固定和试剂浓度。

【例1】HeLa细胞的形态观察

将1mg的FDA溶解在2ml的DMSO中(储存溶液)

用PBS(-)溶液稀释，设为2μmol/L。(用5ml PBS(-)稀释8μl贮存溶液)

1)用胰蛋白酶-EDTA等回收HaLa细胞，制备细胞悬浊液。

2)离心分离细胞悬浊液(1000rpm, 3min)

3)除去上清，添加PBS(-)(此时细胞数为105 ~ 106cells /mL)

4)通过粘贴等充分分散。

*因为培养液中的血清等有使背景上升的危险重复②~④的操作充分清洗。

5)在1.5 mL微管中添加用4)得到的30μL细胞悬浊液。

6)在此加入15μL的FDA溶液。

7)盖上微管，37℃下孵育15min。

8)在玻璃罩上放上7)的溶液10μL，从上面再盖上一层玻璃，夹入染色的细胞悬浊液。

9)将盖杯置于荧光显微镜下，用490nm滤光器激发，观察发出黄绿色荧光的活细胞。

例2:C57BL/6小鼠脾脏细胞的双重染色，使用FDA和PI。

k.h.j ones, j.a. senft, J.Histochem.Cytochem.， 33, 77 (1985).

将5mg的FDA溶解在1ml的丙酮中(储备溶液)

将上述0.04 mL溶液稀释为10ml PBS(-)。

PI将1mg溶解在50ml PBS(-)中。

在0.2 mL D-PBS溶液中混浊的细胞(2×106 cells/mL)溶液中添加0.1 mL的FDA溶液和0.03 mL的PI溶液。

孵化后观察。

【例3】使用HeLa细胞的细胞膜离子梯度对Fluorescein溶出的影响

·E.Prosperi, Histochem. J.， 22, 227 (1990).

将5mg的FDA溶解在1ml的丙酮中(储备溶液)

用PBS(-)，终浓度2.4μmol/L染色。
 

Q2：细胞染料的激发波长和发射波长是多少。
A2：

FDA试剂货号：F209 Fluorescein diacetate

Betulin diacetate(Synonyms: Betulin 3,28-diacetate)

发表于由

上海金畔生物科技有限公司提供天然产物萜类及其苷类Terpenoids and Glycosides。

Betulin diacetate (Synonyms: Betulin 3,28-diacetate) 纯度: ≥95.0%

Betulin diacetate 是一个三萜类化合物和 Betulin 衍生物，具有抗 AID 和抗癌活性。

Betulin diacetate(Synonyms: Betulin 3,28-diacetate)

Betulin diacetate Chemical Structure

CAS No. : 1721-69-3

规格 价格 是否有货 数量
10 mg ￥500 In-stock
25 mg ￥900 In-stock
50 mg ￥1400 In-stock
100 mg ￥2100 In-stock
200 mg   询价  
500 mg   询价  

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Betulin diacetate 相关产品

•相关化合物库:

  • Natural Product Library Plus
  • Bioactive Compound Library Plus

生物活性

Betulin diacetate, a triterpene and derivative of Betulin, is an anti-AID agent and also possesses anti-cancer activity[1][2].

分子量

526.79

Formula

C34H54O4
CAS 号

1721-69-3
运输条件

Room temperature in continental US; may vary elsewhere.
储存方式

4°C, protect from light

*In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)
溶解性数据
In Vitro: 

Ethanol : 5 mg/mL (9.49 mM; Need ultrasonic)

DMSO : < 1 mg/mL (ultrasonic;warming;heat to 60°C) (insoluble or slightly soluble)

配制储备液
浓度 溶剂体积 质量 1 mg 5 mg 10 mg
1 mM 1.8983 mL 9.4914 mL 18.9829 mL
5 mM 0.3797 mL 1.8983 mL 3.7966 mL
10 mM — — —

*

请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液；一旦配成溶液，请分装保存，避免反复冻融造成的产品失效。
储备液的保存方式和期限：-80°C, 6 months; -20°C, 1 month (protect from light)。-80°C 储存时，请在 6 个月内使用，-20°C 储存时，请在 1 个月内使用。
参考文献

  • [1]. I C Sun, et al. Anti-AIDS agents. 34. Synthesis and structure-activity relationships of betulin derivatives as anti-HIV agents. J Med Chem. 1998 Nov 5;41(23):4648-57.

    [2]. T P Shakhtshneider, et al. New composites of betulin esters with arabinogalactan as highly potent anti-cancer agents. Nat Prod Res. 2016 Jun;30(12):1382-7.

Fluorescein Diacetate(Synonyms: 荧光素二乙酸酯; 3,6-Diacetoxyfluoran; Di-O-acetylfluorescein)

发表于由

生化分析试剂 Biochemical Assay Reagents
Fluorescein Diacetate;(Synonyms: 荧光素二乙酸酯; 3,6-Diacetoxyfluoran; Di-O-acetylfluorescein) 纯度: 99.91%

Fluorescein diacetate 是一种细胞渗透性的酯酶底物。Fluorescein diacetate 可作为人谷胱甘肽硫转移酶 Pi (hGSTP1-1) 的荧光底物。

Fluorescein Diacetate(Synonyms: 荧光素二乙酸酯; 3,6-Diacetoxyfluoran; Di-O-acetylfluorescein)

Fluorescein Diacetate Chemical Structure

CAS No. : 596-09-8

规格 价格 是否有货 数量
Free Sample (0.1-0.5 mg) ; Apply now ;
10;mM;*;1 mL in DMSO ￥500 In-stock
500 mg ￥400 In-stock
5 g ￥500 In-stock
10 g ; 询价 ;
50 g ; 询价 ;

* Please select Quantity before adding items.

Fluorescein Diacetate 相关产品

bull;相关化合物库:

  • Bioactive Compound Library Plus

生物活性

Fluorescein diacetate is a cell permeable esterase-substrate. Fluorescein diacetate can be used as a fluorogenic substrate for hGSTP1-1.

体外研究
(In Vitro)

Fluorescein diacetate (FDA) is an acetylated derivative of the green fluorescent dye fluorescein[1]. Fluorescein diacetate (FDA), a fluorescent probe used for vital staining, is a fluorescently activated by esterolytic activity of human Pi-class glutathione S-transferase (hGSTP1) selectively among various cytosolic GSTs. Fluorescence activation of Fluorescein diacetate susceptible to GST inhibitors is observed in MCF7 cells exogenously overexpressing hGSTP1, but not in cells overexpressing hGSTA1 or hGSTM1. Fluorescein diacetate can be used as a fluorogenic substrate for hGSTP1-1. To investigate whether the fluorescence activation is due to hGSTP1 activity, Fluorescein diacetate is incubated with recombinant hGSTP1-1 and GSH in vitro. Remarkable fluorescence activation is observed in the presence of both hGSTP1-1 and GSH, whereas only slight activation is observed in the absence of either of them or when the enzyme is heat inactivated. This suggests that the fluorescence activation of Fluorescein diacetate depends on hGSTP1-1 activity. From the linear relationship between the rate of increase in fluorescence and the hGSTP1-1 concentration, the specific activity of the enzyme for 1 μM Fluorescein diacetate is determined to be 79±15 nmol/min/mg protein. Fluorescein diacetate is applicable as a fluorogenic substrate for evaluating inhibitors of GSTP1-1 in vitro. For Fluorescein diacetate as a substrate, both Ethacrynic acid (EA) and NBDHEX suppress the hGSTP1-1-dependent fluorescent increase in a concentration-dependent manner, with IC50s of 3.3±0.5 μM and 0.61±0.04 μM, respectively[2].

MCE has not independently confirmed the accuracy of these methods. They are for reference only.
分子量

416.38

Formula

C24H16O7
CAS 号

596-09-8
中文名称

二乙酸荧光素；荧光素二乙酸盐；荧光素二乙酸酯
运输条件

Room temperature in continental US; may vary elsewhere.
储存方式

-20deg;C, protect from light

*In solvent : -80deg;C, 6 months; -20deg;C, 1 month (protect from light)
溶解性数据
In Vitro:;

DMSO : 26.67 mg/mL (64.05 mM; Need ultrasonic)

配制储备液
浓度 溶剂体积 质量 1 mg 5 mg 10 mg
1 mM 2.4017 mL 12.0083 mL 24.0165 mL
5 mM 0.4803 mL 2.4017 mL 4.8033 mL
10 mM 0.2402 mL 1.2008 mL 2.4017 mL

*

请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液；一旦配成溶液，请分装保存，避免反复冻融造成的产品失效。
储备液的保存方式和期限：-80°C, 6 months; -20°C, 1 month (protect from light)。-80°C 储存时，请在 6 个月内使用，-20°C 储存时，请在 1 个月内使用。

In Vivo:

请根据您的实验动物和给药方式选择适当的溶解方案。以下溶解方案都请先按照 In Vitro 方式配制澄清的储备液，再依次添加助溶剂：

——为保证实验结果的可靠性，澄清的储备液可以根据储存条件，适当保存；体内实验的工作液，建议您现用现配，当天使用； 以下溶剂前显示的百
分比是指该溶剂在您配制终溶液中的体积占比；如在配制过程中出现沉淀、析出现象，可以通过加热和/或超声的方式助溶

  • 1.

    请依序添加每种溶剂：;10% DMSO ;; 90% (20% SBE-β-CD in saline)

    Solubility: ≥ 2.08 mg/mL (5.00 mM); Clear solution

    此方案可获得 ≥ 2.08 mg/mL (5.00 mM，饱和度未知) 的澄清溶液。

    以 1 mL 工作液为例，取 100 μL 20.8 mg/mL 的澄清 DMSO 储备液加到 900 μL 20% 的 SBE-β-CD 生理盐水水溶液中，混合均匀。

    将 2 g 磺丁基醚 β-环糊精加入 5 mL 生理盐水中，再用生理盐水定容至 10 mL，完全溶解，澄清透明
  • 2.

    请依序添加每种溶剂：;10% DMSO ;; 90% corn oil

    Solubility: ≥ 2.08 mg/mL (5.00 mM); Clear solution

    此方案可获得 ≥ 2.08 mg/mL (5.00 mM，饱和度未知) 的澄清溶液，此方案不适用于实验周期在半个月以上的实验。

    以 1 mL 工作液为例，取 100 μL 20.8 mg/mL 的澄清 DMSO 储备液加到 900 μL玉米油中，混合均匀。

*以上所有助溶剂都可在 MCE 网站选购。
参考文献

  • [1]. Boyd V, et al. Limitations in the Use of Fluorescein Diacetate/Propidium Iodide (FDA/PI) and Cell PermeableNucleic Acid Stains for Viability Measurements of Isolated Islets of Langerhans. Curr Trends Biotechnol Pharm. 2008 Mar;2(2):66-84.

    [2]. Fujikawa Y, et al. Fluorescein diacetate (FDA) and its analogue as substrates for Pi-class glutathione S-transferase (GSTP1) and their biological application. Talanta. 2018 Mar 1;179:845-852.
Cell Assay
[2]

MCF7 cells (2-3×105) are seeded in a 35-mm glass bottom dish before the experiment. Prior to imaging, cells are washed with 1 mL PBS, then incubated in 1 mL Hanks’ Balanced Salt solution (HBSS(+) without phenol red) containing 1 μM Fluorescein diacetate (0.1% DMSO as a cosolvent) for 5 min at 37°C. Cells are washed twice with 1 mL PBS and 1 mL HBSS is added before imaging. Fluorescence images of fluorescein and DsRed-Express2 were acquired in the FITC channel (excitation at 473 nm) and the DsRed channel (excitation 559 nm). The 16-bit images obtained are analysed[2].

MCE has not independently confirmed the accuracy of these methods. They are for reference only.
参考文献

  • [1]. Boyd V, et al. Limitations in the Use of Fluorescein Diacetate/Propidium Iodide (FDA/PI) and Cell PermeableNucleic Acid Stains for Viability Measurements of Isolated Islets of Langerhans. Curr Trends Biotechnol Pharm. 2008 Mar;2(2):66-84.

    [2]. Fujikawa Y, et al. Fluorescein diacetate (FDA) and its analogue as substrates for Pi-class glutathione S-transferase (GSTP1) and their biological application. Talanta. 2018 Mar 1;179:845-852.

5-CFDA(Synonyms: 5-羧基荧光素二乙酸酯 5-Carboxyfluorescein diacetate)

发表于由

5-CFDA;(Synonyms: 5-羧基荧光素二乙酸酯; 5-Carboxyfluorescein diacetate) 纯度: ge;95.0%

5-CFDA(5-Carboxyfluorescein diacetate)是一种可渗入细胞并被水解为5-carboxyfluorescein，用于标记人椎间盘细胞以便体外荧光显微镜观察。

5-CFDAamp;;(Synonyms: 5-羧基荧光素二乙酸酯; 5-Carboxyfluorescein diacetate)

5-CFDA Chemical Structure

CAS No. : 79955-27-4

规格 价格 是否有货 数量
10;mM;*;1 mL in DMSO ￥550 In-stock
50 mg ￥500 In-stock
100 mg ; 询价 ;
200 mg ; 询价 ;

* Please select Quantity before adding items.

5-CFDA 相关产品

bull;相关化合物库:

  • Bioactive Compound Library Plus

生物活性

5-CFDA(5-Carboxyfluorescein diacetate) is membrane-permeant and can be loaded into cells via incubation; hydrolyzed by intracellular esterases to 5-carboxyfluorescein; used for labeling human intervertebral disk cells in vitro for fluorescence microscopy.

分子量

460.39

Formula

C25H16O9
CAS 号

79955-27-4
中文名称

5-羧基荧光素二乙酸酯
运输条件

Room temperature in continental US; may vary elsewhere.
储存方式

-20deg;C, protect from light

*In solvent : -80deg;C, 6 months; -20deg;C, 1 month (protect from light)
溶解性数据
In Vitro:;

DMSO : ≥ 100 mg/mL (217.21 mM)

* “≥” means soluble, but saturation unknown.

配制储备液
浓度 溶剂体积 质量 1 mg 5 mg 10 mg
1 mM 2.1721 mL 10.8604 mL 21.7207 mL
5 mM 0.4344 mL 2.1721 mL 4.3441 mL
10 mM 0.2172 mL 1.0860 mL 2.1721 mL

*

请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液；一旦配成溶液，请分装保存，避免反复冻融造成的产品失效。
储备液的保存方式和期限：-80°C, 6 months; -20°C, 1 month (protect from light)。-80°C 储存时，请在 6 个月内使用，-20°C 储存时，请在 1 个月内使用。

In Vivo:

请根据您的实验动物和给药方式选择适当的溶解方案。以下溶解方案都请先按照 In Vitro 方式配制澄清的储备液，再依次添加助溶剂：

——为保证实验结果的可靠性，澄清的储备液可以根据储存条件，适当保存；体内实验的工作液，建议您现用现配，当天使用； 以下溶剂前显示的百
分比是指该溶剂在您配制终溶液中的体积占比；如在配制过程中出现沉淀、析出现象，可以通过加热和/或超声的方式助溶

  • 1.

    请依序添加每种溶剂：;10% DMSO ;; 40% PEG300 ;; 5% Tween-80 ;; 45% saline

    Solubility: ≥ 2.5 mg/mL (5.43 mM); Clear solution

    此方案可获得 ≥ 2.5 mg/mL (5.43 mM，饱和度未知) 的澄清溶液。

    以 1 mL 工作液为例，取 100 μL 25.0 mg/mL 的澄清 DMSO 储备液加到 400 μL PEG300 中，混合均匀；向上述体系中加入50 μL Tween-80，混合均匀；然后继续加入 450 μL生理盐水定容至 1 mL。

    将 0.9 g 氯化钠，完全溶解于 100 mL ddH₂O 中，得到澄清透明的生理盐水溶液

  • 2.

    请依序添加每种溶剂：;10% DMSO ;; 90% (20% SBE-β-CD in saline)

    Solubility: ≥ 2.5 mg/mL (5.43 mM); Clear solution

    此方案可获得 ≥ 2.5 mg/mL (5.43 mM，饱和度未知) 的澄清溶液。

    以 1 mL 工作液为例，取 100 μL 25.0 mg/mL 的澄清 DMSO 储备液加到 900 μL 20% 的 SBE-β-CD 生理盐水水溶液中，混合均匀。

    将 2 g 磺丁基醚 β-环糊精加入 5 mL 生理盐水中，再用生理盐水定容至 10 mL，完全溶解，澄清透明
  • 3.

    请依序添加每种溶剂：;10% DMSO ;; 90% corn oil

    Solubility: ≥ 2.5 mg/mL (5.43 mM); Clear solution

    此方案可获得 ≥ 2.5 mg/mL (5.43 mM，饱和度未知) 的澄清溶液，此方案不适用于实验周期在半个月以上的实验。

    以 1 mL 工作液为例，取 100 μL 25.0 mg/mL 的澄清 DMSO 储备液加到 900 μL玉米油中，混合均匀。

*以上所有助溶剂都可在 MCE 网站选购。

6-CFDA(Synonyms: 6-羧基荧光素二醋酸 6-Carboxyfluorescein diacetate)

发表于由

6-CFDA;(Synonyms: 6-羧基荧光素二醋酸; 6-Carboxyfluorescein diacetate) 纯度: 98.09%

6-CFDA (6-Carboxyfluorescein diacetate) 是一种可用于流式细胞术和荧光显微镜的荧光探针。6-CFDA 也可以用于活体多光子显微镜研究动物肝胆代谢。

6-CFDAamp;;(Synonyms: 6-羧基荧光素二醋酸; 6-Carboxyfluorescein diacetate)

6-CFDA Chemical Structure

CAS No. : 3348-03-6

规格 价格 是否有货 数量
10;mM;*;1 mL in DMSO ￥660 In-stock
10 mg ￥600 In-stock
50 mg ￥800 In-stock
100 mg ￥1400 In-stock
200 mg ; 询价 ;
500 mg ; 询价 ;

* Please select Quantity before adding items.

6-CFDA 相关产品

bull;相关化合物库:

  • Bioactive Compound Library Plus

生物活性

6-CFDA (6-Carboxyfluorescein diacetate) is a fluorescent probe that can be used in flow cytometry and fluorescence microscopy. 6-CFDA also can be applied in intravital multiphoton microscopy to research hepatobiliary metabolism in animals[1][2][3][4].

分子量

460.39

Formula

C25H16O9
CAS 号

3348-03-6
中文名称

6-羧基荧光素二醋酸
运输条件

Room temperature in continental US; may vary elsewhere.
储存方式

-20deg;C, protect from light

*In solvent : -80deg;C, 6 months; -20deg;C, 1 month (protect from light)
溶解性数据
In Vitro:;

DMSO : 100 mg/mL (217.21 mM; Need ultrasonic)

配制储备液
浓度 溶剂体积 质量 1 mg 5 mg 10 mg
1 mM 2.1721 mL 10.8604 mL 21.7207 mL
5 mM 0.4344 mL 2.1721 mL 4.3441 mL
10 mM 0.2172 mL 1.0860 mL 2.1721 mL

*

请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液；一旦配成溶液，请分装保存，避免反复冻融造成的产品失效。
储备液的保存方式和期限：-80°C, 6 months; -20°C, 1 month (protect from light)。-80°C 储存时，请在 6 个月内使用，-20°C 储存时，请在 1 个月内使用。

In Vivo:

请根据您的实验动物和给药方式选择适当的溶解方案。以下溶解方案都请先按照 In Vitro 方式配制澄清的储备液，再依次添加助溶剂：

——为保证实验结果的可靠性，澄清的储备液可以根据储存条件，适当保存；体内实验的工作液，建议您现用现配，当天使用； 以下溶剂前显示的百
分比是指该溶剂在您配制终溶液中的体积占比；如在配制过程中出现沉淀、析出现象，可以通过加热和/或超声的方式助溶

  • 1.

    请依序添加每种溶剂：;10% DMSO ;; 40% PEG300 ;; 5% Tween-80 ;; 45% saline

    Solubility: ≥ 2.5 mg/mL (5.43 mM); Clear solution

    此方案可获得 ≥ 2.5 mg/mL (5.43 mM，饱和度未知) 的澄清溶液。

    以 1 mL 工作液为例，取 100 μL 25.0 mg/mL 的澄清 DMSO 储备液加到 400 μL PEG300 中，混合均匀；向上述体系中加入50 μL Tween-80，混合均匀；然后继续加入 450 μL生理盐水定容至 1 mL。

    将 0.9 g 氯化钠，完全溶解于 100 mL ddH₂O 中，得到澄清透明的生理盐水溶液

  • 2.

    请依序添加每种溶剂：;10% DMSO ;; 90% (20% SBE-β-CD in saline)

    Solubility: ≥ 2.5 mg/mL (5.43 mM); Clear solution

    此方案可获得 ≥ 2.5 mg/mL (5.43 mM，饱和度未知) 的澄清溶液。

    以 1 mL 工作液为例，取 100 μL 25.0 mg/mL 的澄清 DMSO 储备液加到 900 μL 20% 的 SBE-β-CD 生理盐水水溶液中，混合均匀。

    将 2 g 磺丁基醚 β-环糊精加入 5 mL 生理盐水中，再用生理盐水定容至 10 mL，完全溶解，澄清透明
  • 3.

    请依序添加每种溶剂：;10% DMSO ;; 90% corn oil

    Solubility: ≥ 2.5 mg/mL (5.43 mM); Clear solution

    此方案可获得 ≥ 2.5 mg/mL (5.43 mM，饱和度未知) 的澄清溶液，此方案不适用于实验周期在半个月以上的实验。

    以 1 mL 工作液为例，取 100 μL 25.0 mg/mL 的澄清 DMSO 储备液加到 900 μL玉米油中，混合均匀。

*以上所有助溶剂都可在 MCE 网站选购。
参考文献

  • [1]. Hong-Liang Gao, et al. Vielanin P enhances the cytotoxicity of doxorubicin via the inhibition of PI3K/Nrf2-stimulated MRP1 expression in MCF-7 and K562 DOX-resistant cell lines. Phytomedicine. 2019 May;58:152885.

    [2]. Nobuyasu Yamaguchi, et al. Rapid On-Site Monitoring of Bacteria in Freshwater Environments Using a Portable Microfluidic Counting System. Biol Pharm Bull. 2020;43(1):87-92.

    [3]. Jay Sibbitts , et al. Measuring stimulation and inhibition of intracellular nitric oxide production in SIM-A9 microglia using microfluidic single-cell analysis. Anal Methods. 2020 Oct 8;12(38):4665-4673.

    [4]. Feng-Chieh Li, et al. Dynamic visualization of the recovery of mouse hepatobiliary metabolism to acetaminophen-overdose damage. J Biophotonics. 2019 Mar;12(3):e201800296.

Dihydrofluorescein diacetate(Synonyms: 二氢荧光素二乙酸酯)

发表于由

Dihydrofluorescein diacetate;(Synonyms: 二氢荧光素二乙酸酯) 纯度: 99.13%

Dihydrofluorescein diacetate是用于无细胞系统和细胞模型中测量氧化应激的荧光探针。

Dihydrofluorescein diacetateamp;;(Synonyms: 二氢荧光素二乙酸酯)

Dihydrofluorescein diacetate Chemical Structure

CAS No. : 35340-49-9

规格 价格 是否有货 数量
Free Sample (0.1-0.5 mg) ; Apply now ;
10;mM;*;1 mL in DMSO ￥790 In-stock
50 mg ￥600 In-stock
100 mg ; 询价 ;
200 mg ; 询价 ;

* Please select Quantity before adding items.

Dihydrofluorescein diacetate 相关产品

bull;相关化合物库:

  • Bioactive Compound Library Plus
  • Anti-Aging Compound Library
  • Oxygen Sensing Compound Library
  • Anti-Parkinson’s Disease Compound Library
  • Neurodegenerative Disease-related Compound Library

生物活性

Dihydrofluorescein diacetate is a fluorimetric probe mainly used for oxidative stress measurements, in both cell-free systems and cellular models.

体外研究
(In Vitro)

Dihydrofluorescein diacetate may be a superior fluorescent probe for many cell-based studies. It is a better fluorescent probe for detecting intracellular oxidants because it is more reactive toward specific oxidizing species. Dihydrofluorescein diacetate demonstrates fluorescence of linear structures, consistent with mitochondria, in reoxygenated endothelium[1]. Dihydrofluorescein diacetate is able to detect the presence of ROS in mitochondria. Dihydrofluorescein diacetate fluorescence was sharp and delineated thin filaments which corresponded in all details to TMRM-stained mitochondria. It enters mitochondria and reacts with ROS released in the matrix[2]. Dihydrofluorescein diacetate could be an useful and quantitative method for measuring the oxidative potential of nanoparticle-treated cells[3].

MCE has not independently confirmed the accuracy of these methods. They are for reference only.
分子量

418.40

Formula

C24H18O7
CAS 号

35340-49-9
中文名称

二氢荧光素二乙酸酯
运输条件

Room temperature in continental US; may vary elsewhere.
储存方式

4deg;C, protect from light

*In solvent : -80deg;C, 6 months; -20deg;C, 1 month (protect from light)
溶解性数据
In Vitro:;

DMSO : ≥ 106.5 mg/mL (254.54 mM)

* “≥” means soluble, but saturation unknown.

配制储备液
浓度 溶剂体积 质量 1 mg 5 mg 10 mg
1 mM 2.3901 mL 11.9503 mL 23.9006 mL
5 mM 0.4780 mL 2.3901 mL 4.7801 mL
10 mM 0.2390 mL 1.1950 mL 2.3901 mL

*

请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液；一旦配成溶液，请分装保存，避免反复冻融造成的产品失效。
储备液的保存方式和期限：-80°C, 6 months; -20°C, 1 month (protect from light)。-80°C 储存时，请在 6 个月内使用，-20°C 储存时，请在 1 个月内使用。
参考文献

  • [1]. Hempel SL, et al. Dihydrofluorescein diacetate is superior for detecting intracellular oxidants: comparison with 2′,7′-dichlorodihydrofluorescein diacetate, 5(and 6)-carboxy-2′,7′-dichlorodihydrofluorescein diacetate, and dihydrorhodamine 123. Free Radic Biol Med. 1999 Jul;27(1-2):146-59.

    [2]. Diaz G, et al. Mitochondrial localization of reactive oxygen species by dihydrofluorescein probes. Histochem Cell Biol. 2003 Oct;120(4):319-25.

    [3]. Aranda A, et al. Dichloro-dihydro-fluorescein diacetate (DCFH-DA) assay: a quantitative method for oxidative stress assessment of nanoparticle-treated cells. Toxicol In Vitro. 2013 Mar;27(2):954-63.
Cell Assay

Dihydrofluorescein diacetate is used at a final concentration of 5 μM for the cell-free studies. The excitation wavelength is 488 nm and the emission wavelength is 521 nm, the same as the excitation and emission wavelengths of the confocal microscope. All fluorescence measurements are performed on room temperature solutions. Human umbilical vein endothelium at first passage are cultured to confluence on 48 well plates. Cells are ished with PBS, then PBS with glucose, pH 7.4, is added to the cells. Dihydrofluorescein diacetate is added at a final concentration of 20 mM. Wells without cells are also loaded with fluorescent probes to serve as controls. Fluorescence measurements are made on a fluorescent platereader every 10 min at Exλ 488 nm and Emλ 510 (bandpass 5 nm) and 538 nm (bandpass 10 nm). The Emλ 510 nm and Emλ 538 nm values are added together[1].

MCE has not independently confirmed the accuracy of these methods. They are for reference only.
参考文献

  • [1]. Hempel SL, et al. Dihydrofluorescein diacetate is superior for detecting intracellular oxidants: comparison with 2′,7′-dichlorodihydrofluorescein diacetate, 5(and 6)-carboxy-2′,7′-dichlorodihydrofluorescein diacetate, and dihydrorhodamine 123. Free Radic Biol Med. 1999 Jul;27(1-2):146-59.

    [2]. Diaz G, et al. Mitochondrial localization of reactive oxygen species by dihydrofluorescein probes. Histochem Cell Biol. 2003 Oct;120(4):319-25.

    [3]. Aranda A, et al. Dichloro-dihydro-fluorescein diacetate (DCFH-DA) assay: a quantitative method for oxidative stress assessment of nanoparticle-treated cells. Toxicol In Vitro. 2013 Mar;27(2):954-63.

5(6)-CFDA(Synonyms: 5(6)-羧基荧光素二乙酸酯 5-(6)-Carboxyfluorescein diacetate CFDA)

发表于由

5(6)-CFDA;(Synonyms: 5(6)-羧基荧光素二乙酸酯; 5-(6)-Carboxyfluorescein diacetate; CFDA) 纯度: 98.05%

5(6)-CFDA (5-(6)-Carboxyfluorescein diacetate) 是一种细胞渗透性、胺反应型绿色荧光探针，用于细胞染色分析。(λex: 492 nm; λem: 517 nm)。

5(6)-CFDAamp;;(Synonyms: 5(6)-羧基荧光素二乙酸酯; 5-(6)-Carboxyfluorescein diacetate; CFDA)

5(6)-CFDA Chemical Structure

CAS No. : 124387-19-5

规格 价格 是否有货 数量
10;mM;*;1 mL in DMSO ￥880 In-stock
50 mg ￥800 In-stock
100 mg ￥1400 In-stock
200 mg ; 询价 ;
500 mg ; 询价 ;

* Please select Quantity before adding items.

5(6)-CFDA 相关产品

bull;相关化合物库:

  • Bioactive Compound Library Plus

生物活性

5(6)-CFDA (5-(6)-Carboxyfluorescein diacetate) is a cell-permeant, amine-reactive green fluorescent probe used to stain cells for analysis in applications. (λex: 492 nm; λem: 517 nm)[1].

分子量

460.39

Formula

C50H32O18
CAS 号

124387-19-5
中文名称

5(6)-羧基荧光素二乙酸酯；5-(6)-羧基荧光素双醋酸盐；5-(6)-羧基荧光素二醋酸盐
运输条件

Room temperature in continental US; may vary elsewhere.
储存方式

-20deg;C, protect from light

*In solvent : -80deg;C, 6 months; -20deg;C, 1 month (protect from light)
溶解性数据
In Vitro:;

DMSO : 125 mg/mL (271.51 mM; Need ultrasonic)

配制储备液
浓度 溶剂体积 质量 1 mg 5 mg 10 mg
1 mM 2.1721 mL 10.8604 mL 21.7207 mL
5 mM 0.4344 mL 2.1721 mL 4.3441 mL
10 mM 0.2172 mL 1.0860 mL 2.1721 mL

*

请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液；一旦配成溶液，请分装保存，避免反复冻融造成的产品失效。
储备液的保存方式和期限：-80°C, 6 months; -20°C, 1 month (protect from light)。-80°C 储存时，请在 6 个月内使用，-20°C 储存时，请在 1 个月内使用。

In Vivo:

请根据您的实验动物和给药方式选择适当的溶解方案。以下溶解方案都请先按照 In Vitro 方式配制澄清的储备液，再依次添加助溶剂：

——为保证实验结果的可靠性，澄清的储备液可以根据储存条件，适当保存；体内实验的工作液，建议您现用现配，当天使用； 以下溶剂前显示的百
分比是指该溶剂在您配制终溶液中的体积占比；如在配制过程中出现沉淀、析出现象，可以通过加热和/或超声的方式助溶

  • 1.

    请依序添加每种溶剂：;10% DMSO ;; 40% PEG300 ;; 5% Tween-80 ;; 45% saline

    Solubility: ≥ 2.08 mg/mL (4.52 mM); Clear solution

    此方案可获得 ≥ 2.08 mg/mL (4.52 mM，饱和度未知) 的澄清溶液。

    以 1 mL 工作液为例，取 100 μL 20.8 mg/mL 的澄清 DMSO 储备液加到 400 μL PEG300 中，混合均匀；向上述体系中加入50 μL Tween-80，混合均匀；然后继续加入 450 μL生理盐水定容至 1 mL。

    将 0.9 g 氯化钠，完全溶解于 100 mL ddH₂O 中，得到澄清透明的生理盐水溶液

  • 2.

    请依序添加每种溶剂：;10% DMSO ;; 90% (20% SBE-β-CD in saline)

    Solubility: ≥ 2.08 mg/mL (4.52 mM); Clear solution

    此方案可获得 ≥ 2.08 mg/mL (4.52 mM，饱和度未知) 的澄清溶液。

    以 1 mL 工作液为例，取 100 μL 20.8 mg/mL 的澄清 DMSO 储备液加到 900 μL 20% 的 SBE-β-CD 生理盐水水溶液中，混合均匀。

    将 2 g 磺丁基醚 β-环糊精加入 5 mL 生理盐水中，再用生理盐水定容至 10 mL，完全溶解，澄清透明
*以上所有助溶剂都可在 MCE 网站选购。
参考文献

  • [1]. Yang T, et al. A novel nonradioactive CFDA assay to monitor the cellular immune response in myeloid leukemia. Immunobiology. 2013 Apr;218(4):548-53.

    [2]. Card SD, et al. Assessment of fluorescein-based fluorescent dyes for tracing Neotyphodium endophytes in planta. Mycologia. 2013 Jan-Feb;105(1):221-9.

    [3]. Fang X, et al. Bone marrow-derived endothelial progenitor cells are involved in aneurysm repair in rabbits. J Clin Neurosci. 2012 Sep;19(9):1283-6.