Dihydrorhodamine 123 (DHR 123) is a fluorescent probe (λ_ex=488 nm, λ_em=525 nm)^[1].

In the presence of 10 μM Dihydrorhodamine 123 (DHR 123) the stimulation of the neutrophil NADPH oxidase by the addition of 50 nM phorbol 12-myristate 13-acetat (PMA) resultes in an increase in the rate of rhodamine generation. The fluorescent intensity of the cells, in the presence of 10 μM Dihydrorhodamine 123, increases with time following the addition of 50 nM PMA. In the presence of 10 μM Dihydrorhodamine 123, induced HL60 cells show a sustained increase in fluorescence following the addition of 50 nM PMA^[1].

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

346.38

C₂₁H₁₈N₂O₃

109244-58-8

Room temperature in continental US; may vary elsewhere.

-20deg;C, protect from light

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In Vitro:;

DMSO : 100 mg/mL (288.70 mM; Need ultrasonic)

*

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• [1]. Lydia M. Henderson et al. Dihydrorhodamine 123: a fluorescent probe for superoxide generation? Eur.J.Biochem. 217, 973-980.

The HL60 cells are incubated at 6×10⁶ cells/mL in Krebs-Ringer buffer at 37°C containing 10 μM Dihydrorhodamine 123 (DHR). The generation of O₂^– is initiated by the addition of 50 nM phorbol 12-myristate 13-acetat (PMA) and the progress of the generation of rhodamine 123 is monitored in 50-μL aliquots (3×10⁵ cells) diluted tenfold before analysis. The uninduced HL60 cells are loaded with 5 μM carboxy SNARF-1 AM acetate (SNARF-AM) in the Na⁺ medium for 10 min at 37°C and washed by centrifugation and resuspension to remove unhydrolysed SNARF ester^[1].

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

• [1]. Lydia M. Henderson et al. Dihydrorhodamine 123: a fluorescent probe for superoxide generation? Eur.J.Biochem. 217, 973-980.