标签归档：devd

Z-DEVD-AMC

发表于由

Z-DEVD-AMC;

Z-DEVD-AMC 是一种选择性 caspase-3 底物，可通过荧光光谱法测量。 AMC 可用作基于 AMC 的酶底物 (包括基于 AMC 的半胱天冬酶底物) 的荧光参考标准。

Z-DEVD-AMCamp;;

Z-DEVD-AMC Chemical Structure

规格 是否有货
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生物活性

Z-DEVD-AMC is a selective caspase-3 substrate that can be measured by fluorescence spectrometry. AMC can be used as a fluorescence reference standard for AMC-based enzyme substrates including AMC-based caspase substrates[1].

分子量

767.74

Formula

C36H41N5O14
运输条件

Room temperature in continental US; may vary elsewhere.
储存方式

Please store the product under the recommended conditions in the Certificate of Analysis.
参考文献

  • [1]. Mauro C X Pinto, et al. Discovery of cytotoxic and pro-apoptotic compounds against leukemia cells: Tert-butyl-4-[(3-nitrophenoxy) methyl]-2,2-dimethyloxazolidine-3-carboxylate. Life Sci. 2011 Nov 21;89(21-22):786-94.

Z-DEVD-AFC

发表于由

Z-DEVD-AFC;

Z-DEVD-AFC 是细胞渗透的 caspase-3 的底物，它会导致 AFC 荧光团的荧光向上移动。Z-DEVD-AFC 可用于检测 caspase-3 样酶的活性。

Z-DEVD-AFCamp;;

Z-DEVD-AFC Chemical Structure

CAS No. : 1135416-11-3

规格 是否有货
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250 mg ; 询价 ;
500 mg ; 询价 ;

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生物活性

Z-DEVD-AFC is a cell-permeant substrate for caspase-3, which causes a shift in fluorescence uponcleavage of the AFC fluorophore. Z-DEVD-AFC can be used to detect caspase-3-like enzymes activity[1].

分子量

821.71

Formula

C36H38F3N5O14
CAS 号

1135416-11-3
Sequence Shortening

zDEVD-AFC
运输条件

Room temperature in continental US; may vary elsewhere.
储存方式

Please store the product under the recommended conditions in the Certificate of Analysis.
参考文献

  • [1]. Santiago AR, et al. High glucose induces caspase-independent cell death in retinal neural cells. Neurobiol Dis. 2007;25(3):464-472.

荧光染料FITC-C6-DEVD-FMK

发表于由

荧光染料Dye Reagents FITC-C6-DEVD-FMK;

FITC-C6-DEVD-FMK 是一种 Caspase-3 荧光标记抑制剂。可用于检测哺乳动物细胞凋亡中的活性 caspase-3。Z-DEVD-FMK 是一种特异性 caspase-3 抑制剂。

FITC-C6-DEVD-FMK

FITC-C6-DEVD-FMK Chemical Structure

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100 mg ; 询价 ;
250 mg ; 询价 ;
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生物活性

FITC-C6-DEVD-FMK, a fluorescently labeled caspase-3 inhibitor, can be used for detection of active caspase-3 in mammalian cells undergoing apoptosis. FITC-C6-DEVD-FMK provides a convenient means for sensitive detection of activated caspase-3 in living cells. Z-DEVD-FMK is a specific caspase-3 inhibitor[1].

分子量

994.99

Formula

C46H51FN6O16S
运输条件

Room temperature in continental US; may vary elsewhere.
储存方式

Please store the product under the recommended conditions in the Certificate of Analysis.
参考文献

  • [1]. Kanthasamy AG, et al. A novel peptide inhibitor targeted to caspase-3 cleavage site of a proapoptotic kinase protein kinase C delta (PKCdelta) protects against dopaminergic neuronal degeneration in Parkinson’s disease models. Free Radic Biol Med. 2006 Nov

Ac-DEVD-AMC

发表于由

Ac-DEVD-AMC; 纯度: 99.45%

Ac-DEVD-AMC 是 Caspase-3 的底物。

Ac-DEVD-AMCamp;;

Ac-DEVD-AMC Chemical Structure

CAS No. : 169332-61-0

规格 价格 是否有货 数量
1 mg ￥500 In-stock
5 mg ￥1500 In-stock
10 mg ; 询价 ;
50 mg ; 询价 ;

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Ac-DEVD-AMC 相关产品

bull;相关化合物库:

  • Bioactive Compound Library Plus
  • Peptidomimetic Library
  • Peptide Library

生物活性

Ac-DEVD-AMC is the Caspase-3 substrate.

体外研究
(In Vitro)

Ac-DEVD-AMC is the caspase-3 substrate and could be used to monitor intracellular caspase-3 activity[1].The AMC moiety is highest at 2 h (area =5.6±1.8), the AMC peak area for Ac-DEVD-AMC incubated without lung specimen (substrate alone) for 6 h is relatively small[2].

MCE has not independently confirmed the accuracy of these methods. They are for reference only.
分子量

675.64

Formula

C30H37N5O13
CAS 号

169332-61-0
Sequence

N-Acetyl-Asp-Glu-Val-Asp-7-amido-4-Methylcoumarin
Sequence Shortening

Ac-DEVD-7-amido-4-Methylcoumarin
运输条件

Room temperature in continental US; may vary elsewhere.
储存方式

Protect from light

Powder -80deg;C 2 years
-20deg;C 1 year

*In solvent : -80deg;C, 6 months; -20deg;C, 1 month (protect from light)
溶解性数据
In Vitro:;

H2O : 0.67 mg/mL (0.99 mM; Need ultrasonic)
参考文献

  • [1]. Ahmed R Alsuwaidi, et al. Bioenergetics of murine lungs infected with respiratory syncytial virus. Virol J. 2013; 10: 22.

    [2]. Ahmed R Alsuwaidi, et al. Lung tissue bioenergetics and caspase activity in rodents. BMC Res Notes. 2013; 6: 12.
Cell Assay
[2]

Lung fragments (about 20 mg each) are collected from rats and mice. The samples are incubated at 37°C in 50 mL KH buffer or MEM (continuously gass with 95% O2 : 5% CO2) for up to 6 h. At specific time points, samples are incubated in oxygenated KH buffer or MEM with 32 μM zVAD-fmk or 15 μL DMSO for 20 min (f/v=1.0 mL). Ac-DEVD-AMC (37 μM) is then added and the incubation continues for additional 20 min. At the end of incubation, the tissue is disrupted by vigorous homogenization for 2 min, sonication for 3 min and 10 passages through a 27-G needle. This disruption procedure quenches the Ac-DEVD-AMC cleavage reaction due to dilution. The supernatants are collected by centrifugation (~6,300g for 90 min) through microcentrifuge filter, separateed on HPLC, and analyzed for the fluorogenic AMC moiety[2].

MCE has not independently confirmed the accuracy of these methods. They are for reference only.
参考文献

  • [1]. Ahmed R Alsuwaidi, et al. Bioenergetics of murine lungs infected with respiratory syncytial virus. Virol J. 2013; 10: 22.

    [2]. Ahmed R Alsuwaidi, et al. Lung tissue bioenergetics and caspase activity in rodents. BMC Res Notes. 2013; 6: 12.