CFSE;(Synonyms: 5(6)-Carboxyfluorescein diacetate succinimidyl ester; CFDA-SE; 5(6)-CFDA N-succinmidyl ester) 纯度: 99.01%
CFSE (5(6)-Carboxyfluorescein diacetate succinimidyl ester) 是一种细胞内荧光染料,可追踪细胞增殖。共价结合的 CFSE 在子细胞之间平均分配,从而可以区分连续的细胞分裂。
CFSE Chemical Structure
CAS No. : 150347-59-4
规格 | 价格 | 是否有货 | 数量 |
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5 mg | ¥800 | In-stock | |
10 mg | ¥1400 | In-stock | |
50 mg | ; | 询价 | ; |
100 mg | ; | 询价 | ; |
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CFSE 相关产品
bull;相关化合物库:
- Bioactive Compound Library Plus
生物活性 |
CFSE (5(6)-Carboxyfluorescein diacetate succinimidyl ester) is an intracellular fluorescent dye which can track proliferating cells. Covalently bound CFSE is divided equally between daughter cells, allowing discrimination of successive rounds of cell division[1][2]. |
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体外研究 (In Vitro) |
CFSE is a fluorescent dye which can track the cell division[1]. To examine the ability of immune cells to migrate into live slices, a single-cell suspension of autologous splenocytes or PBMC is isolated, labeling them with CFSE, and adding them to the top of the slices on day 2 of culture. After 6 additional days of culture, the CFSE-labeling immune cells (green) are found to have migrated throughout the slices. No positive cells are found in the slices without the addition of CFSE-labeling immune cells[2]. MCE has not independently confirmed the accuracy of these methods. They are for reference only. |
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分子量 |
557.46 |
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Formula |
C58H38N2O22 |
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CAS 号 |
150347-59-4 |
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中文名称 |
5(6)-羧基二乙酸荧光素 N-琥珀酰亚胺酯 |
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运输条件 |
Room temperature in continental US; may vary elsewhere. |
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储存方式 |
-20deg;C, sealed storage, away from moisture and light *In solvent : -80deg;C, 6 months; -20deg;C, 1 month (sealed storage, away from moisture and light) |
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溶解性数据 |
In Vitro:;
DMSO : 50 mg/mL (89.69 mM; Need ultrasonic) 配制储备液
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请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液;一旦配成溶液,请分装保存,避免反复冻融造成的产品失效。 In Vivo:
请根据您的实验动物和给药方式选择适当的溶解方案。以下溶解方案都请先按照 In Vitro 方式配制澄清的储备液,再依次添加助溶剂: ——为保证实验结果的可靠性,澄清的储备液可以根据储存条件,适当保存;体内实验的工作液,建议您现用现配,当天使用; 以下溶剂前显示的百
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参考文献 |
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Cell Assay [2] |
Single-cell suspensions of splenocytes (or PBMC) are stained with 1 µM CFSE in PBS for 9 min at 37°C, combined with 20 mL of 10% FBS RPMI-1640 medium at RT for 2 min, centrifuged, washed, and counted. 2×106 CFSE-labeling splenocytes are added to the top of the slices in 24-well membrane culture insert[2]. MCE has not independently confirmed the accuracy of these methods. They are for reference only. |
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参考文献 |
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