上海金畔生物科技有限公司代理日本同仁化学 DOJINDO代理商全线产品,欢迎访问官网了解更多信息
C16H18N2Na2O14S2
572.43
上海金畔生物科技有限公司代理日本同仁化学 DOJINDO代理商全线产品,欢迎访问官网了解更多信息
C16H18N2Na2O14S2
572.43
上海金畔生物科技有限公司代理日本同仁化学 DOJINDO代理商全线产品,欢迎访问官网了解更多信息
上海金畔生物代理Hampton research品牌蛋白结晶试剂耗材工具等,我们将竭诚为您服务,欢迎访问Hampton research官网或者咨询我们获取更多相关Hampton research品牌产品信息。
Products > Optimize Reagents > Optimize – Buffers > BIS-TRIS Buffer
Synonyms: 2,2-Bis(hydroxymethyl)-2,2′,2″”-nitrilotriethanol, or 2-Bis(2-hydroxyethyl)amino-2-(hydroxymethyl)-1,3-propanediol, or Bis(2-hydroxyethyl)amino-tris(hydroxymethyl)methane.
C8H19NO5
Mr 209.24
CAS Number [6976-37-0]
EC Number 230-237-7
Beilstein Registry Number 2205275
MDL Number MFCD00002853
Purity ≥ 98.0%
pKa = 6.4 at 20°C
pKa = 6.5 at 25°C
HR2-781 is titrated to pH 5.5 using HCl
Measured Conductivity Range of HR2-781: 38.4 – 43.1 mS/cm at 25°C
Measured Refractive Index of HR2-781: 1.37069 – 1.37150 at 20°C
HR2-783 is titrated to pH 6.5 using HCl
Measured Conductivity Range of HR2-783: 22.8 – 28.7 mS/cm at 25°C
Measured Refractive Index of HR2-783: 1.36817 – 1.36926 at 20°C
同义词:2,2-双(羟甲基)-2,2′,2″”-次氮基三乙醇,或 2-双(2-羟乙基)氨基-2-(羟甲基)-1,3-丙二醇,或双(2- 羟乙基)氨基-三(羟甲基)甲烷。
C8H19NO5
209.24 先生
CAS 编号 [6976-37-0]
欧盟编号 230-237-7
贝尔斯坦注册号 2205275
MDL 编号 MFCD00002853
纯度≥98.0%
pKa = 6.4 在 20°C
pKa = 6.5 在 25°C
使用 HCl 将 HR2-781 滴定至 pH 5.5
HR2-781 的测量电导率范围:25°C 时为 38.4 – 43.1 mS/cm
HR2-781 的实测折射率:20°C 时为 1.37069 – 1.37150
HR2-783 使用 HCl 滴定至 pH 6.5
HR2-783 的测量电导率范围:22.8 – 28.7 mS/cm 在 25°C
HR2-783 的实测折射率:20°C 时为 1.36817 – 1.36926
Al ≤0.0005%
Ca ≤0.0005%
Cl ≤0.05%
Cu ≤0.0005%
Fe ≤0.0005%
K ≤0.005%
Mg ≤0.0005%
NH4 ≤0.05%
Na ≤0.005%
Pb ≤0.001%
SO4 ≤0.05%
Zn ≤0.0005%
BIS-TRIS
HR2-781
100 mL
$169.00
HR2-783
100 mL
$169.00
1. Atomic structure of the degraded procapsid particle of the bacteriophage G4: induced structural changes in the presence of calcium ions and functional implications. McKenna, R; Bowman, BR; Ilag, LL; Rossmann, MG; Fane, BA. J Mol Biol 256, 736- 750, 1996.
2. Expression, crystallization and preliminary X-ray diffraction study of FtsY, the docking protein of the signal recognition particle of E. coli. Montoya, G; Svensson, C; Luirink, J; Sinning, I. Proteins 28, 285- 288, 1997.
1. 噬菌体 G4 降解前衣壳颗粒的原子结构:在钙离子存在下诱导结构变化和功能影响。 麦肯纳,R; 鲍曼,BR; 伊拉格,LL; 罗斯曼,MG; 法恩,文学士。 分子生物学杂志 256, 736-750, 1996。
2.大肠杆菌信号识别颗粒对接蛋白FtsY的表达、结晶和初步X射线衍射研究。 蒙托亚,G; 斯文森,C; 卢林克,J; Sinning, I. 蛋白质 28, 285-288, 1997。
上海金畔生物科技有限公司代理日本同仁化学 DOJINDO代理商全线产品,欢迎访问官网了解更多信息
上海金畔生物代理Hampton research品牌蛋白结晶试剂耗材工具等,我们将竭诚为您服务,欢迎访问Hampton research官网或者咨询我们获取更多相关Hampton research品牌产品信息。
Products > Optimize Reagents > Optimize – Buffers > BIS-TRIS propane Buffer
Synonyms: 1,3-Bis[tris(hydroxymethyl)methylamino]propane
C11H26N2O6
CH2[CH2NHC(CH2OH)3]2
Mr 282.34
CAS Number [64431-96-5]
EC Number 264-899-3
Beilstein Registry Number 1786109
MDL Number MFCD00004689
PubChem Substance ID 24891934
Purity ≥ 99.0%
pKa1 = 6.8 at 25°C
pKa2 = 9.0 at 25°C
HR2-795 is titrated to pH 7.0 using HCl
Measured Conductivity Range: 41.9 – 43.5 mS/cm at 25°C
Measured Refractive Index Range: 1.39110 – 1.39222 at 20°C
别名:1,3-双[三(羟甲基)甲氨基]丙烷
C11H26N2O6
CH2[CH2NHC(CH2OH)3]2
282.34 先生
CAS 编号 [64431-96-5]
欧盟编号 264-899-3
贝尔斯坦注册号 1786109
MDL 编号 MFCD00004689
PubChem 物质 ID 24891934
纯度≥99.0%
pKa1 = 6.8 在 25°C
pKa2 = 9.0 在 25°C
HR2-795 使用 HCl 滴定至 pH 7.0
测得的电导率范围:25°C 时 41.9 – 43.5 mS/cm
测得的折射率范围:20°C 时为 1.39110 – 1.39222
BIS-TRIS propane
HR2-795
100 mL
$108.00
1. Two-fold symmetry of crystalline DNA-EcoRI endonuclease recognition complexes. Grable, J; Frederick, CA; Samudzi, C; Jen-Jacobson, L; Lesser, D; Greene, P; Boyer, HW; Itakura, K; Rosenberg, JM. J Biomol Struct Dyn 1, 1149- 1160, 1984.
2. Non-native Local Interactions in Protein Folding and Stability: Introducing a Helical Tendency in the AII beta-Sheet alpha-Spectrin SH3 Domain. Prieto, J; Wilmans, M; Jimenez, MA; Rico, M; Serrano, L. J Mol Biol 268, 760- 778, 1997.
1. 结晶 DNA-EcoRI 核酸内切酶识别复合物的双重对称性。 格拉布尔,J; 加利福尼亚州弗雷德里克; 萨穆兹,C; 仁-雅各布森,L; 较小,D; 格林,P; 博耶,HW; 板仓,K; 罗森伯格,JM。 J Biomol Struct Dyn 1, 1149-1160, 1984。
2. 蛋白质折叠和稳定性中的非天然局部相互作用:在 AII β-片 α-Spectrin SH3 域中引入螺旋趋势。 普列托,J; 威尔曼斯,M; 马萨诸塞州希门尼斯; 里科,M; Serrano, L. J Mol Biol 268, 760-778, 1997。
上海金畔生物科技有限公司代理日本同仁化学 DOJINDO代理商全线产品,欢迎访问官网了解更多信息
上海金畔生物代理Hampton research品牌蛋白结晶试剂耗材工具等,我们将竭诚为您服务,欢迎访问Hampton research官网或者咨询我们获取更多相关Hampton research品牌产品信息。
Products > Optimize Reagents > Optimize – Buffers > Citric acid BIS-TRIS propane Buffer
Citrate BIS-TRIS propane (CBTP) is a buffer system useful across pH 2.5 to 9.5.
Using CBTP one can use a single buffer system to screen pH 2.5 to 9.5 by varying the ratio of Citric acid to BIS-TRIS propane. Although the two buffer reagents are made available separately for convenient, individual replacement, they are designed to be used together, with the supplied CBTP buffer titration table to create a crystallization buffer system covering pH 2.5 to 9.5.
HR2-831 Measured pH range: 1.2 – 1.5 at 25°C
HR2-831 Measured Conductivity Range: 7.7 – 8.7 mS/cm at 25°C
HR2-833 Measured pH range: 11.2 – 11.5 at 25°C
HR2-833 Measured Conductivity Range: 298 – 317 µS/cm at 25°C
HR2-833 Measured Refractive Index Range: 1.38000 – 1.38076 at 20°C
柠檬酸盐 BIS-TRIS 丙烷 (CBTP) 是一种适用于 pH 2.5 至 9.5 的缓冲系统。
使用 CBTP,可以使用单一缓冲系统通过改变柠檬酸与 BIS-TRIS 丙烷的比例来筛选 pH 2.5 至 9.5。 虽然这两种缓冲试剂是分开提供的,以便于单独更换,但它们被设计为一起使用,与提供的 CBTP 缓冲液滴定表一起创建一个涵盖 pH 2.5 至 9.5 的结晶缓冲系统。
HR2-831 测得的 pH 值范围:25°C 时为 1.2 – 1.5
HR2-831 测量电导率范围:25°C 时 7.7 – 8.7 mS/cm
HR2-833 测得的 pH 范围:25°C 时为 11.2 – 11.5
HR2-833 测量的电导率范围:298 – 317 µS/cm,25°C
HR2-833 测量的折射率范围:20°C 时为 1.38000 – 1.38076
Citric acid
HR2-831
100 mL
$45.00
HR2-833
100 mL
$162.00
荧光染料Dye Reagents Hydroxystilbamidine bis(methanesulfonate); 纯度: ge;96.0%
Hydroxystilbamidine bis(methanesulfonate) 是一种能够结合 DNA 和 RNA 的染料, 也被发现是细胞核糖核酸酶的强效抑制剂。
Hydroxystilbamidine bis(methanesulfonate) Chemical Structure
CAS No. : 223769-64-0
规格 | 价格 | 是否有货 | 数量 |
---|---|---|---|
1 mg | ¥850 | In-stock | |
5 mg | ; | 询价 | ; |
10 mg | ; | 询价 | ; |
* Please select Quantity before adding items.
生物活性 |
Hydroxystilbamidine bis(methanesulfonate), a dye capable of binding to both DNA and RNA, has been found to be a powerful inhibitor of cellular ribonucleases. |
---|---|
体外研究 (In Vitro) |
It is found that the trypanocidal dye Hydroxystilbamidine bis(methanesulfonate) permits the recovery of mRNA after polysome released with Nonidet P-40 (NP-40). Sucrose gradient analysis of detergent-lysed postnuclear supernates is used to analyze the size distribution of NP-40-released polysomes. The heparin gradient shows some polyribosomes, whereas the Hydroxystilbamidine bis(methanesulfonate) gradient shows a remarkably large peak of very heavy polyribosomes. This peak is obtained reproducibly if Hydroxystilbamidine bis(methanesulfonate) is present before the addition of NP-40[1]. MCE has not independently confirmed the accuracy of these methods. They are for reference only. |
体内研究 (In Vivo) |
Hydroxystilbamidine bis(methanesulfonate) is an effective suppressor of the plaque-forming cell (PFC) response when given before sheep erythrocytes (SRBC) stimulation. Hydroxystilbamidine bis(methanesulfonate) depresses the plaque response of the treated mice. Fewer PFC are observed in Hydroxystilbamidine bis(methanesulfonate)-treated mice throughout the experiment, but the level of suppression decreases with time. By day 14, the number of PFC observed in both the Hydroxystilbamidine bis(methanesulfonate) treated mice and the control group is essentially at the background level[2]. MCE has not independently confirmed the accuracy of these methods. They are for reference only. |
分子量 |
472.54 |
Formula |
C18H24N4O7S2 |
CAS 号 |
223769-64-0 |
运输条件 |
Room temperature in continental US; may vary elsewhere. |
储存方式 |
-20deg;C, sealed storage, away from moisture and light *In solvent : -80deg;C, 6 months; -20deg;C, 1 month (sealed storage, away from moisture and light) |
参考文献 |
|
Kinase Assay [1] |
Silkwork larvae are used in this study. Larvae on the fourth or fifth day of the fifth instar (~4.2 g body weight) are injected with 35 μL of a solution of 10 mg/mL cyclobeximidein H2O. After 5 min, the animals are immobilized in ice, and posterior silk glands are dissected and washed in ice-cold 0.15 M NaCl, 0.015 M Na citrate, 100 μg/mL cycloheximide. Washed glands from two larvae are placed in a homogenizer containing 4.7 mL of 40 mM triethanolamine-HCl, pH 7.5, 0.15 M sucrose, 0.1 M KCI, 3 mM MgCl2, 2 mM reduced glutathione, 10 μg/mL cycloheximide, 750 μg/mL Escherichia coli tRNA, and an appropriate concentration of RNase inhibitor (sodium heparin, 1.5 μg/mL or Hydroxystilbamidine bis(methanesulfonate), 1.5 mM)[1]. MCE has not independently confirmed the accuracy of these methods. They are for reference only. |
---|---|
Animal Administration [2] |
Mice (six per group) are given various doses of Hydroxystilbamidine bis(methanesulfonate) (HSB) 3, 2, and 1 day before antigen. Other groups are given Hydroxystilbamidine bis(methanesulfonate) 1 or 2 days after the injection of antigen. Another group of mice receive antigen and Hydroxystilbamidine bis(methanesulfonate) simultaneously. A control group receives only antigen. The antigen dose consists of 2×108 sheep erythrocytes (SRBC). Four days after the injection of SRBC, the mice are sacrificed and spleens are removed and assayed for plaque-forming cell (PFC) by the plaque assay[2]. MCE has not independently confirmed the accuracy of these methods. They are for reference only. |
参考文献 |
|
荧光染料Dye Reagents Hydroxystilbamidine bis(methanesulfonate); 纯度: ge;96.0%
Hydroxystilbamidine bis(methanesulfonate) 是一种能够结合 DNA 和 RNA 的染料, 也被发现是细胞核糖核酸酶的强效抑制剂。
Hydroxystilbamidine bis(methanesulfonate) Chemical Structure
CAS No. : 223769-64-0
规格 | 价格 | 是否有货 | 数量 |
---|---|---|---|
1 mg | ¥850 | In-stock | |
5 mg | ; | 询价 | ; |
10 mg | ; | 询价 | ; |
* Please select Quantity before adding items.
生物活性 |
Hydroxystilbamidine bis(methanesulfonate), a dye capable of binding to both DNA and RNA, has been found to be a powerful inhibitor of cellular ribonucleases. |
---|---|
体外研究 (In Vitro) |
It is found that the trypanocidal dye Hydroxystilbamidine bis(methanesulfonate) permits the recovery of mRNA after polysome released with Nonidet P-40 (NP-40). Sucrose gradient analysis of detergent-lysed postnuclear supernates is used to analyze the size distribution of NP-40-released polysomes. The heparin gradient shows some polyribosomes, whereas the Hydroxystilbamidine bis(methanesulfonate) gradient shows a remarkably large peak of very heavy polyribosomes. This peak is obtained reproducibly if Hydroxystilbamidine bis(methanesulfonate) is present before the addition of NP-40[1]. MCE has not independently confirmed the accuracy of these methods. They are for reference only. |
体内研究 (In Vivo) |
Hydroxystilbamidine bis(methanesulfonate) is an effective suppressor of the plaque-forming cell (PFC) response when given before sheep erythrocytes (SRBC) stimulation. Hydroxystilbamidine bis(methanesulfonate) depresses the plaque response of the treated mice. Fewer PFC are observed in Hydroxystilbamidine bis(methanesulfonate)-treated mice throughout the experiment, but the level of suppression decreases with time. By day 14, the number of PFC observed in both the Hydroxystilbamidine bis(methanesulfonate) treated mice and the control group is essentially at the background level[2]. MCE has not independently confirmed the accuracy of these methods. They are for reference only. |
分子量 |
472.54 |
Formula |
C18H24N4O7S2 |
CAS 号 |
223769-64-0 |
运输条件 |
Room temperature in continental US; may vary elsewhere. |
储存方式 |
-20deg;C, sealed storage, away from moisture and light *In solvent : -80deg;C, 6 months; -20deg;C, 1 month (sealed storage, away from moisture and light) |
参考文献 |
|
Kinase Assay [1] |
Silkwork larvae are used in this study. Larvae on the fourth or fifth day of the fifth instar (~4.2 g body weight) are injected with 35 μL of a solution of 10 mg/mL cyclobeximidein H2O. After 5 min, the animals are immobilized in ice, and posterior silk glands are dissected and washed in ice-cold 0.15 M NaCl, 0.015 M Na citrate, 100 μg/mL cycloheximide. Washed glands from two larvae are placed in a homogenizer containing 4.7 mL of 40 mM triethanolamine-HCl, pH 7.5, 0.15 M sucrose, 0.1 M KCI, 3 mM MgCl2, 2 mM reduced glutathione, 10 μg/mL cycloheximide, 750 μg/mL Escherichia coli tRNA, and an appropriate concentration of RNase inhibitor (sodium heparin, 1.5 μg/mL or Hydroxystilbamidine bis(methanesulfonate), 1.5 mM)[1]. MCE has not independently confirmed the accuracy of these methods. They are for reference only. |
---|---|
Animal Administration [2] |
Mice (six per group) are given various doses of Hydroxystilbamidine bis(methanesulfonate) (HSB) 3, 2, and 1 day before antigen. Other groups are given Hydroxystilbamidine bis(methanesulfonate) 1 or 2 days after the injection of antigen. Another group of mice receive antigen and Hydroxystilbamidine bis(methanesulfonate) simultaneously. A control group receives only antigen. The antigen dose consists of 2×108 sheep erythrocytes (SRBC). Four days after the injection of SRBC, the mice are sacrificed and spleens are removed and assayed for plaque-forming cell (PFC) by the plaque assay[2]. MCE has not independently confirmed the accuracy of these methods. They are for reference only. |
参考文献 |
|
Bis-ANS dipotassium;
Bis-ANS dipotassium 是一种蛋白质荧光探针,Bis-ANS 结合到 tubulin,Kd 为 2 μM。Bis-ANS dipotassium 是一种有效的蛋白质液-液相分离 (LLPS) 的双相调节剂。Bis-ANS dipotassium 低浓度促进 LLPS,高浓度抑制 LLPS。
Bis-ANS dipotassium Chemical Structure
CAS No. : 65664-81-5
规格 | 是否有货 | ||
---|---|---|---|
100 mg | ; | 询价 | ; |
250 mg | ; | 询价 | ; |
500 mg | ; | 询价 | ; |
* Please select Quantity before adding items.
生物活性 |
Bis-ANS dipotassium is a fluorescent probe of hydrophobic protein. Bis-ANS binds to tubulin with a Kd of 2 μM[1]. Bis-ANS dipotassium is a potent biphasic modulator of protein liquid-liquid phase separation (LLPS). Bis-ANS dipotassium promotes LLPS at low concentrations but suppresses LLPS at high concentrations[2]. |
---|---|
体外研究 (In Vitro) |
Bis-ANS is a fluorescent molecule that has been used to probe exposed hydrophobic patches in proteins and to monitor the formation of protein aggregates. Bis-ANS is a bivalent naphthalene sulfonate with aniline moieties. Bis-ANS modulates the liquid-liquid phase separation of TDP-43 low complexity domain (LCD)[2]. MCE has not independently confirmed the accuracy of these methods. They are for reference only. |
分子量 |
672.85 |
Formula |
C32H22K2N2O6S2 |
CAS 号 |
65664-81-5 |
运输条件 |
Room temperature in continental US; may vary elsewhere. |
储存方式 |
Please store the product under the recommended conditions in the Certificate of Analysis. |
参考文献 |
|
生化分析试剂 Biochemical Assay Reagents
Bis-Tris;
Bis-Tris 是一种常用的缓冲液。
Bis-Tris Chemical Structure
CAS No. : 6976-37-0
规格 | 价格 | 是否有货 | 数量 |
---|---|---|---|
Free Sample (0.1-0.5 mg) | ; | Apply now | ; |
25 g | ¥500 | In-stock | |
50 g | ; | 询价 | ; |
* Please select Quantity before adding items.
生物活性 |
Bis-Tris is a commonly used buffer[1]. |
||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
分子量 |
209.24 |
||||||||||||||||
Formula |
C8H19NO5 |
||||||||||||||||
CAS 号 |
6976-37-0 |
||||||||||||||||
运输条件 |
Room temperature in continental US; may vary elsewhere. |
||||||||||||||||
储存方式 |
|
||||||||||||||||
溶解性数据 |
In Vitro:;
H2O : 500 mg/mL (2389.60 mM; Need ultrasonic) 配制储备液
*
请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液;一旦配成溶液,请分装保存,避免反复冻融造成的产品失效。 |
||||||||||||||||
参考文献 |
|
品牌:AIST
CAS No.:725-89-3 储存条件:室温 纯度:– |
产品编号
(生产商编号) |
等级 | 规格 | 运输包装 | 零售价(RMB) | 库存情况 | 参考值 |
634-29181 |
– | 200 mg | – | 咨询 | – | – |
* 干冰运输、大包装及大批量的产品需酌情添加运输费用
* 零售价、促销产品折扣、运输费用、库存情况、产品及包装规格可能因各种原因有所变动,恕不另行通知,确切详情请联系上海金畔生物科技有限公司。
品牌:AIST
CAS No.:16956-91-5 储存条件:2-10℃ 纯度:– |
产品编号
(生产商编号) |
等级 | 规格 | 运输包装 | 零售价(RMB) | 库存情况 | 参考值 |
639-44151 |
– | 100 mg | – | 咨询 | – | – |
* 干冰运输、大包装及大批量的产品需酌情添加运输费用
* 零售价、促销产品折扣、运输费用、库存情况、产品及包装规格可能因各种原因有所变动,恕不另行通知,确切详情请联系上海金畔生物科技有限公司。