标签归档：biotin

Anti HLA class I-A,B,C-biotin, Mouse Monoclonal Antibody 品牌：Hokudo

发表于由

Anti HLA class I-A,B,C-biotin, Mouse Monoclonal Antibody

品牌：Hokudo
CAS No.：
储存条件：2-10℃
纯度：–
产品编号

(生产商编号)

 等级 规格 运输包装 零售价(RMB) 库存情况 参考值

304-97781

 – 100uL – 咨询 – –


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Hyaluronan Binding Protein, Recombinant Biotin Conjugate 品牌：Hokudo

发表于由

Hyaluronan Binding Protein, Recombinant Biotin Conjugate

品牌：Hokudo
CAS No.：
储存条件：-20℃
纯度：–
产品编号

(生产商编号)

 等级 规格 运输包装 零售价(RMB) 库存情况 参考值

BC41

 – 200 ul – 咨询 – –


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Biotin-Substance P

发表于由

Biotin-Substance P;

Biotin-Substance P (Neurokinin P) 是一种神经肽，在中枢神经系统中作为神经递质和神经调节剂。Substance P 的内源性受体是神经激肽1受体 (neurokinin 1 receptor，NK1R)

Biotin-Substance Pamp;;

Biotin-Substance P Chemical Structure

CAS No. : 87468-58-4

规格 是否有货
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250 mg ; 询价 ;
500 mg ; 询价 ;

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生物活性

Biotin-Substance P is the biotin tagged Substance P. Substance P (Neurokinin P) is a neuropeptide, acting as a neurotransmitter and as a neuromodulator in the CNS. The endogenous receptor for substance P is neurokinin 1 receptor (NK1-receptor, NK1R)[1].

体外研究
(In Vitro)

The neuropeptide substance P (SP) that are mediated by the neurokinin 1 receptor (NK1-R) desensitize and resensitize, which may be associated with NK1-R endocytosis and recycling. SP and the NK1-R are internalized into the same clathrin immunoreactive vesicles, and then sorted into different compartments. SP is intact at the cell surface and in early endosomes, but slowly degraded in perinuclear vesicles. SP induces clathrin-dependent internalization of the NK1-R. The SP/NK1-R complex dissociates in acidified endosomes. SP is degraded, whereas the NK1-R recycles to the cell surface. SP induces internalization of the NK1-R both in transfected epithelial cells[1].

MCE has not independently confirmed the accuracy of these methods. They are for reference only.
分子量

1574.90

Formula

C73H112N20O15S2
CAS 号

87468-58-4
Sequence Shortening

Biotin-RPKPQQFFGLM-NH2
运输条件

Room temperature in continental US; may vary elsewhere.
储存方式

Please store the product under the recommended conditions in the Certificate of Analysis.
Solvent Solubility
In Vitro:;

H2O

Peptide Solubility and Storage Guidelines:

1.;;Calculate the length of the peptide.

2.;;Calculate the overall charge of the entire peptide according to the following table:

; Contents Assign value
Acidic amino acid Asp (D), Glu (E), and the C-terminal -COOH. -1
Basic amino acid Arg (R), Lys (K), His (H), and the N-terminal -NH2 +1
Neutral amino acid Gly (G), Ala (A), Leu (L), Ile (I), Val (V), Cys (C), Met (M), Thr (T), Ser (S), Phe (F), Tyr (Y), Trp (W), Pro (P), Asn (N), Gln (Q) 0

3.;;Recommended solution:

Overall charge of peptide Details
Negative (lt;0) 1.;;Try to dissolve the peptide in water first.
2.;;If water fails, add NH4OH (lt;50 μL).
3.;;If the peptide still does not dissolve, add DMSO (50-100 μL) to solubilize the peptide.
Positive (gt;0) 1.;;Try to dissolve the peptide in water first.
2.;;If water fails, try dissolving the peptide in a 10%-30% acetic acid solution.
3.;;If the peptide still does not dissolve, try dissolving the peptide in a small amount of DMSO.
Zero (=0) 1.;;Try to dissolve the peptide in organic solvent (acetonitrile, methanol, etc.) first.
2.;;For very hydrophobic peptides, try dissolving the peptide in a small amount of DMSO, and then dilute the solution with water to the desired concentration.
参考文献

  • [1]. Grady EF, et al. Delineation of the endocytic pathway of substance P and its seven-transmembrane domain NK1 receptor. Mol Biol Cell. 1995 May;6(5):509-24.

Peanut Lectin, Biotin Labeled 花生凝集素，生物素标记 品牌：FUJIFILM Wako

发表于由

Peanut Lectin, Biotin Labeled

花生凝集素，生物素标记

品牌：FUJIFILM Wako
CAS No.：
储存条件：-20℃
纯度：–
产品编号

(生产商编号)

 等级 规格 运输包装 零售价(RMB) 库存情况 参考值

169-15671

 for Biochemistry 5 mg –

Peanut Lectin, Biotin Labeled 花生凝集素，生物素标记 品牌：FUJIFILM Wako

 – –


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Syk Kinase Peptide Substrate, Biotin labeled

发表于由

Syk Kinase Peptide Substrate, Biotin labeled;

Syk Kinase Peptide Substrate, Biotin labeled 是生物素标记的、Syk 激酶多肽底物。

Syk Kinase Peptide Substrate, Biotin labeledamp;;

Syk Kinase Peptide Substrate, Biotin labeled Chemical Structure

规格 是否有货
100 mg ; 询价 ;
250 mg ; 询价 ;
500 mg ; 询价 ;

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生物活性

Syk Kinase Peptide Substrate, Biotin labeled is a biotin-labled Syk kinase peptide substrate.

分子量

1689.90

Formula

C76H108N18O24S1
Sequence

Biotin-Lys-Glu-Asp-Pro-Asp-Tyr-Glu-Trp-Pro-Ser-Ala-Lys-NH2
Sequence Shortening

Biotin-KEDPDYEWPSAK-NH2
运输条件

Room temperature in continental US; may vary elsewhere.
储存方式

Please store the product under the recommended conditions in the Certificate of Analysis.
Solvent Solubility
In Vitro:;

H2O

Peptide Solubility and Storage Guidelines:

1.;;Calculate the length of the peptide.

2.;;Calculate the overall charge of the entire peptide according to the following table:

; Contents Assign value
Acidic amino acid Asp (D), Glu (E), and the C-terminal -COOH. -1
Basic amino acid Arg (R), Lys (K), His (H), and the N-terminal -NH2 +1
Neutral amino acid Gly (G), Ala (A), Leu (L), Ile (I), Val (V), Cys (C), Met (M), Thr (T), Ser (S), Phe (F), Tyr (Y), Trp (W), Pro (P), Asn (N), Gln (Q) 0

3.;;Recommended solution:

Overall charge of peptide Details
Negative (lt;0) 1.;;Try to dissolve the peptide in water first.
2.;;If water fails, add NH4OH (lt;50 μL).
3.;;If the peptide still does not dissolve, add DMSO (50-100 μL) to solubilize the peptide.
Positive (gt;0) 1.;;Try to dissolve the peptide in water first.
2.;;If water fails, try dissolving the peptide in a 10%-30% acetic acid solution.
3.;;If the peptide still does not dissolve, try dissolving the peptide in a small amount of DMSO.
Zero (=0) 1.;;Try to dissolve the peptide in organic solvent (acetonitrile, methanol, etc.) first.
2.;;For very hydrophobic peptides, try dissolving the peptide in a small amount of DMSO, and then dilute the solution with water to the desired concentration.

Pam3CSK4-Biotin(Synonyms: Pam3Cys-Ser-(Lys)4-Biotin)

发表于由

Pam3CSK4-Biotin;(Synonyms: Pam3Cys-Ser-(Lys)4-Biotin)

Pam3CSK4-Biotin 是生物素化的 Pam3CSK4。Pam3CSK4-Biotin 是 Toll 样受体 1/2 (TLR1/2) 激动剂。

Pam3CSK4-Biotinamp;;(Synonyms: Pam3Cys-Ser-(Lys)4-Biotin)

Pam3CSK4-Biotin Chemical Structure

规格 是否有货
100 mg ; 询价 ;
250 mg ; 询价 ;
500 mg ; 询价 ;

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生物活性

Pam3CSK4-Biotin is biotinylated Pam3CSK4. Pam3CSK4-Biotin is a Toll-like receptor 1/2 (TLR1/2) agonist[1].

IC50 Target

TLR1

;

TLR2

;

体外研究
(In Vitro)

Pam3CSK4-Biotin (100 ng/mL) induces NF-κB activation from endosomal compartments[1].

MCE has not independently confirmed the accuracy of these methods. They are for reference only.
分子量

1962.85

Formula

C103H192N14O17S2
Sequence Shortening

Pam3C-SKKKK
运输条件

Room temperature in continental US; may vary elsewhere.
储存方式

Please store the product under the recommended conditions in the Certificate of Analysis.
Solvent Solubility
In Vitro:;

H2O

Peptide Solubility and Storage Guidelines:

1.;;Calculate the length of the peptide.

2.;;Calculate the overall charge of the entire peptide according to the following table:

; Contents Assign value
Acidic amino acid Asp (D), Glu (E), and the C-terminal -COOH. -1
Basic amino acid Arg (R), Lys (K), His (H), and the N-terminal -NH2 +1
Neutral amino acid Gly (G), Ala (A), Leu (L), Ile (I), Val (V), Cys (C), Met (M), Thr (T), Ser (S), Phe (F), Tyr (Y), Trp (W), Pro (P), Asn (N), Gln (Q) 0

3.;;Recommended solution:

Overall charge of peptide Details
Negative (lt;0) 1.;;Try to dissolve the peptide in water first.
2.;;If water fails, add NH4OH (lt;50 μL).
3.;;If the peptide still does not dissolve, add DMSO (50-100 μL) to solubilize the peptide.
Positive (gt;0) 1.;;Try to dissolve the peptide in water first.
2.;;If water fails, try dissolving the peptide in a 10%-30% acetic acid solution.
3.;;If the peptide still does not dissolve, try dissolving the peptide in a small amount of DMSO.
Zero (=0) 1.;;Try to dissolve the peptide in organic solvent (acetonitrile, methanol, etc.) first.
2.;;For very hydrophobic peptides, try dissolving the peptide in a small amount of DMSO, and then dilute the solution with water to the desired concentration.
参考文献

  • [1]. Brandt KJ, et al. TLR2 ligands induce NF-κB activation from endosomal compartments of human monocytes. PLoS One. 2013 Dec 12;8(12):e80743.

Concanavalin A, Biotin Labeled 伴刀豆球蛋白A-生物素标记 品牌：FUJIFILM Wako

发表于由

Concanavalin A, Biotin Labeled

伴刀豆球蛋白A-生物素标记

品牌：FUJIFILM Wako
CAS No.：
储存条件： –
纯度：–
产品编号

(生产商编号)

 等级 规格 运输包装 零售价(RMB) 库存情况 参考值

031-14661

 – 5 mg –

Concanavalin A, Biotin Labeled 伴刀豆球蛋白A-生物素标记 品牌：FUJIFILM Wako

 – –


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Biotin-TAT (47-57)

发表于由

Biotin-TAT (47-57);

Biotin-TAT (47-57) 是生物素标记的 TAT 多肽，是转录的反式激活因子。Biotin-TAT (47-57) 是最广泛用于蛋白转导域 (PTDs) 的一个多肽，进入不同的原代细胞是依赖于 ATP 和温度的，表明参与内吞作用。

Biotin-TAT (47-57)amp;;

Biotin-TAT (47-57) Chemical Structure

CAS No. : 1231898-25-1

规格 是否有货
100 mg ; 询价 ;
250 mg ; 询价 ;
500 mg ; 询价 ;

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生物活性

Biotin-TAT (47-57), a biotin tagged TAT, is a transactivator of transcription. Biotin-TAT (47-57) is one of the most widely used protein transduction domains (PTDs) into different primary cells is ATP- and temperature-dependent, indicating the involvement of endocytosis[1][2].

分子量

1786.12

Formula

C74H132N34O16S
CAS 号

1231898-25-1
Sequence Shortening

{Biotin}-YGRKKRRQRRR
运输条件

Room temperature in continental US; may vary elsewhere.
储存方式

Please store the product under the recommended conditions in the Certificate of Analysis.
Solvent Solubility
In Vitro:;

H2O

Peptide Solubility and Storage Guidelines:

1.;;Calculate the length of the peptide.

2.;;Calculate the overall charge of the entire peptide according to the following table:

; Contents Assign value
Acidic amino acid Asp (D), Glu (E), and the C-terminal -COOH. -1
Basic amino acid Arg (R), Lys (K), His (H), and the N-terminal -NH2 +1
Neutral amino acid Gly (G), Ala (A), Leu (L), Ile (I), Val (V), Cys (C), Met (M), Thr (T), Ser (S), Phe (F), Tyr (Y), Trp (W), Pro (P), Asn (N), Gln (Q) 0

3.;;Recommended solution:

Overall charge of peptide Details
Negative (lt;0) 1.;;Try to dissolve the peptide in water first.
2.;;If water fails, add NH4OH (lt;50 μL).
3.;;If the peptide still does not dissolve, add DMSO (50-100 μL) to solubilize the peptide.
Positive (gt;0) 1.;;Try to dissolve the peptide in water first.
2.;;If water fails, try dissolving the peptide in a 10%-30% acetic acid solution.
3.;;If the peptide still does not dissolve, try dissolving the peptide in a small amount of DMSO.
Zero (=0) 1.;;Try to dissolve the peptide in organic solvent (acetonitrile, methanol, etc.) first.
2.;;For very hydrophobic peptides, try dissolving the peptide in a small amount of DMSO, and then dilute the solution with water to the desired concentration.
参考文献

  • [1]. Chen Sun, et al. Intracellular tracking of single native molecules with electroporation-delivered quantum dots. Anal Chem. 2014 Nov 18;86(22):11403-9.

    [2]. Jean Philippe Richard, et al. Cellular uptake of unconjugated TAT peptide involves clathrin-dependent endocytosis and heparan sulfate receptors. J Biol Chem. 2005 Apr 15;280(15):15300-6.

日本同仁化学SulfoBiotics- Biotin-HPDP(WS) solution试剂货号：SB17 生物素标记蛋白质巯基标记试剂（水溶性）| DOJINDO

发表于由

上海金畔生物科技有限公司代理日本同仁化学 DOJINDO代理商全线产品，欢迎访问官网了解更多信息

SulfoBiotics- Biotin-HPDP(WS) solution试剂货号：SB17
生物素标记蛋白质巯基标记试剂（水溶性）
SulfoBiotics- Biotin-HPDP(WS) solution
商品信息
储存条件：0-5度保存
运输条件：室温

下载说明书
SDS下载

选择规格：
500μl

期货

 
硫化氢

关联产品

SulfoBiotics- SSP4试剂
3′,6′-Di(O-thiosalicyl)fluorescein

SulfoBiotics- Biotin-HPDP(WS) solution试剂货号：SB17 生物素标记蛋白质巯基标记试剂（水溶性）
SulfoBiotics- HSip-1试剂
N-​[3′,​6′-Dihydroxy-​3-​oxo-3H-spiro(isobenzofur​an-​1,​9′-​xanthen)-​5-​yl]​-​(1,​4,​7,​10-​tetraazacyclododecan​e-​1-​acetamide-​κN1, κN4, κN7, κN10) ​copper(2+) sulfate

SulfoBiotics- Biotin-HPDP(WS) solution试剂货号：SB17 生物素标记蛋白质巯基标记试剂（水溶性）
SulfoBiotics- HSip-1 DA试剂
N-[3′,6′-Bis(acetyloxy)-3-oxo-3H-spiro(isobenzofuran-1,9′-xanthen)-5-yl]-(1,4,7,10-tetraazacyclododecane-1-acetamide-κN1,κN4,κN7,κN10copper(2+)sulfate

SulfoBiotics- Biotin-HPDP(WS) solution试剂货号：SB17 生物素标记蛋白质巯基标记试剂（水溶性）
SulfoBiotics- GYY4137试剂
(4-Methoxyphenyl)morpholinylphosphinodithioic acid, morpholine salt

SulfoBiotics- Biotin-HPDP(WS) solution试剂货号：SB17 生物素标记蛋白质巯基标记试剂（水溶性）
-SulfoBiotics- Protein Redox State Monitoring Kit Plus
-SulfoBiotics- Protein Redox State Monitoring Kit Plus

在线小工具

实验工具|稀释计算器|摩尔浓度计算器

日本同仁化学Biotin-PEAC5-maleimide试剂货号：B299 CAS号：374592-98-0| DOJINDO

发表于由

上海金畔生物科技有限公司代理日本同仁化学 DOJINDO代理商全线产品，欢迎访问官网了解更多信息

Biotin-PEAC5-maleimide试剂货号：B299
N-6-(Biotinylamino)hexanoyl-N’-[2-(N-maleimido)ethyl]piperazine, hydrochloride
CAS号：374592-98-0
商品信息
储存条件：-20度保存
运输条件：室温
分子式：

C26H41ClN6O5S

分子量：

585.16

下载说明书
宣传资料
SDS下载

选择规格：
10mg

期货

 
蛋白抗体标记检测方案

Biotin-PEAC5-maleimide试剂货号：B299 CAS号：374592-98-0

Biotin-PEAC5-maleimide试剂货号：B299 CAS号：374592-98-0

试剂盒内含
规格性状
产品概述
原理
操作步骤
参考文献

试剂盒内含

Biotin-PEAC5-maleimide试剂货号：B299 CAS号：374592-98-0

规格性状

性状：该产品为白色至微黄色粉末，溶于二甲基亚砜。

纯度（HPLC）：90.0％以上

二甲基亚砜可溶：测试合格

产品概述

已知生物素与抗生物素蛋白牢固结合，其特性已广泛用于高灵敏度分析。由于生物素与抗生物素蛋白之间的结合稳定性常数为1015，比抗原-抗体反应高3至4个数量级，因此一旦结合至抗生物素蛋白的生物素在生理条件下不会脱落。另外，生物素是具有羧基的小分子，可以容易地化学修饰，并且可以在不损害蛋白质等的活性的情况下进行标记。因此，已经开发出许多生物素化合物作为使用抗体进行免疫测定的标记剂。具有活性酯基的生物素通常用于与蛋白质的氨基（NH2基）结合，具有马来酰亚胺基的生物素用于巯基（SH基）。

根据目的，有必要选择在抗生物素蛋白识别的生物素头和反应性基团之间的间隔物的长度，但是通常，间隔物越长，当抗生物素蛋白结合时，对抗体的抗原识别活性越强。可以认为，间隔物的影响可能引起非特异性吸附，并且在测量期间的背景可能很高。因此，为了提高测量灵敏度，请查看S/N（信噪比）。必须选择适当长度的垫片。

间隔基通常使用氨基己酸，并且存在一种化合物，其中一个分子的氨基己酸通过酰胺键被引入生物素中，并且该化合物中的两个分子被引入。由于己酸间隔物变长，水溶性变差，因此通常将生物素标记试剂溶解在DMSO中并添加到含有蛋白质的缓冲液中的方法是普遍的。

当在用氨基标记蛋白质时不能使用有机溶剂时，可以使用具有磺酸基的活性酯化合物。此外，在与巯基的反应中，以相同的方式使用由DMSO制备的溶液，但是由于其在间隔部分具有哌嗪结构，因此通过质子化在中性区域显示水溶性。

用于还原糖的生物素酰肼化合物的水溶性低，需要在DMSO溶液中进行调节。

在ELISA（酶联免疫吸附测定）中，将酶标记的链霉亲和素与生物素标记的抗体结合的方法是常见的。与抗生蛋白链菌素相比，抗生物素蛋白便宜，但很少用于ELISA中。原因是背景极高，并且认为高PI是原因。抗生物素蛋白通常用于通过柱分离生物素结合蛋白等的目的。

原理

Biotin-PEAC5-maleimide试剂货号：B299 CAS号：374592-98-0

操作步骤

产品使用步骤:

1. 制备含巯基的抗体

1) 用PBS溶解DTT (浓度为200mmol/l)，制成DTT溶液。

2) 精确称量1.0-5.0mg抗体并记录，加入500ul 10mmol/l HEPES Buffer (pH8.0) 溶解蛋白质，制成抗体溶液。

3) 在抗体溶液加入2ul DTT溶液，充分混合。

2. 凝胶过滤纯化

1) 打开NAP-5层析柱上的盖子，弃去填充液。

2) 打开层析柱出口的盖子，用PBS分数次洗脱，每根层析柱收集大约10ml的洗脱液，使凝胶平衡。

3) 用移液器吸取500μl标记好的抗体溶液加入到层析柱中，弃去此时的流出液。

4) 在层析柱的出口处放置一根管子，在层析柱中加入1mlPBS，收集流出的抗体。

3. 生物素标记

1) 用DMSO溶解Biotin-PE-maleimide (浓度为50mmol/l，4.7mg/200ul或10mg/424ul)。

＊用Biotin-PEAC5-maleimide时浓度为5.9mg/200ul或10mg/342ul。

2) 在纯化好的抗体溶液中加入配制好的生物素标记试剂溶液，抗体：生物素标记试剂的混合摩尔比为1:10左右。

3) 充分混合后，在水浴恒温摇床 (25℃)中过夜培养。

4. 标记抗体的凝胶过滤纯化

1) 打开NAP-10层析柱上的盖子，弃去填充液。

2) 打开层析柱出口的盖子，用PBS分数次洗脱，每根层析柱收集大约15ml的洗脱液，使凝胶平衡。

3) 用移液器吸取1ml标记好的抗体溶液加入到层析柱中，弃去此时的流出液。

4) 在层析柱的出口处放置一根管子，在层析柱中加入1.5ml PBS，收集流出的生物素标记抗体。

参考文献

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2) E. Ishikawa, M. Imagawa, S. Hashida, S. Toshitake, Y. Hamaguchi and T. Ueno, “Enzyme-labeling of Antibodies and their Fragments for Enzyme Immunoassay and Immunohistochemical Staining”, J. Immunoassay, 1983, 4, 209.

3) H. -J. Friesen, P. Hermentin and P. Gronski, “Novel Maleimido-Biotins for the Selective Biotinylation of Sulfhydrils”, Protides Biol. Fluids, 1987, 34, 43.

4) E. Ishikawa, S. Hashida, T. Kohno, T. Kotani and S. Ohtani, “Modification of Monoclonal Antibodies with Enzymes, Biotin, and Fluorochromes and Their Applications”, Immunol. Ser., 1987, 33, 113.

5) R. B. del Rosalio and R. L. Wahl, “Disulfide Bond-targeted Radiolabeling : Tumor Specificity of a Streptavidine-biotinylated Monoclonal Antibody Complex”, Cancer Res. (Suppl.), 1990, 50, 804S.