标签归档：bh

Bim BH3, Peptide IV

发表于由

Bim BH3, Peptide IV;

Bim BH3, Peptide IV 是一种来自 BH3-only protein Bim 的 26 个残基肽，属于 Bcl-2 蛋白家族的促凋亡家族。

Bim BH3, Peptide IVamp;;

Bim BH3, Peptide IV Chemical Structure

CAS No. : 721885-31-0

规格 是否有货
100 mg ; 询价 ;
250 mg ; 询价 ;
500 mg ; 询价 ;

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生物活性

Bim BH3, Peptide IV is a 26-residue peptide from BH3-only protein Bim, which belongs to the pro-apoptotic group of the Bcl-2 family of proteins[1][2].

分子量

3269.65

Formula

C145H222N44O41S
CAS 号

721885-31-0
Sequence Shortening

DMRPEIWIAQELRRIGDEFNAYYARR
运输条件

Room temperature in continental US; may vary elsewhere.
储存方式

Please store the product under the recommended conditions in the Certificate of Analysis.
参考文献

  • [1]. Zhu Y, et al. Constitutive association of the proapoptotic protein Bim with Bcl-2-related proteins on mitochondriain T cells. Proc Natl Acad Sci U S A. 2004 May 18;101(20):7681-6.

    [2]. Lee EF, et al. High-resolution structural characterization of a helical alpha/beta-peptide foldamer bound to the anti-apoptotic protein Bcl-xL. Angew Chem Int Ed Engl. 2009;48(24):4318-22.

Angiopep-Bim BH3 hydrochloride

发表于由

Angiopep-Bim BH3 hydrochloride;

Angiopep-Bim BH3 hydrochloride 是能透过血脑屏障的多肽，可用于研究中枢神经系统的通透性。

Angiopep-Bim BH3 hydrochlorideamp;;

Angiopep-Bim BH3 hydrochloride Chemical Structure

规格 是否有货
100 mg ; 询价 ;
250 mg ; 询价 ;
500 mg ; 询价 ;

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生物活性

Angiopep-Bim BH3 hydrochloride, a BBB penetrated peptode, could be used to investigate the permeability of CNS therapeutics[1].

分子量

6273.28

Formula

C277H415ClN82O82S
Sequence Shortening

TFFYGGSRGKRNNFKTEEYLPSTGGGGGDMRPEIWIAQELRRIGDEFNAYYARR
运输条件

Room temperature in continental US; may vary elsewhere.
储存方式

Please store the product under the recommended conditions in the Certificate of Analysis.
Solvent Solubility
In Vitro:;

H2O

Peptide Solubility and Storage Guidelines:

1.;;Calculate the length of the peptide.

2.;;Calculate the overall charge of the entire peptide according to the following table:

; Contents Assign value
Acidic amino acid Asp (D), Glu (E), and the C-terminal -COOH. -1
Basic amino acid Arg (R), Lys (K), His (H), and the N-terminal -NH2 +1
Neutral amino acid Gly (G), Ala (A), Leu (L), Ile (I), Val (V), Cys (C), Met (M), Thr (T), Ser (S), Phe (F), Tyr (Y), Trp (W), Pro (P), Asn (N), Gln (Q) 0

3.;;Recommended solution:

Overall charge of peptide Details
Negative (lt;0) 1.;;Try to dissolve the peptide in water first.
2.;;If water fails, add NH4OH (lt;50 μL).
3.;;If the peptide still does not dissolve, add DMSO (50-100 μL) to solubilize the peptide.
Positive (gt;0) 1.;;Try to dissolve the peptide in water first.
2.;;If water fails, try dissolving the peptide in a 10%-30% acetic acid solution.
3.;;If the peptide still does not dissolve, try dissolving the peptide in a small amount of DMSO.
Zero (=0) 1.;;Try to dissolve the peptide in organic solvent (acetonitrile, methanol, etc.) first.
2.;;For very hydrophobic peptides, try dissolving the peptide in a small amount of DMSO, and then dilute the solution with water to the desired concentration.
参考文献

  • [1]. Sonja Bergmann, et al. Blood-brain-barrier organoids for investigating the permeability of CNS therapeutics. Nat Protoc. 2018 Dec;13(12):2827-2843.

Bax BH3 peptide (55-74), wild type

发表于由

Bax BH3 peptide (55-74), wild type;

Bax BH3 peptide (55-74), wild type 是一种含 20 个氨基酸的 Bax BH3 肽 (Bax 1)，能够在多种细胞系模型中诱导细胞凋亡。

Bax BH3 peptide (55-74), wild typeamp;;

Bax BH3 peptide (55-74), wild type Chemical Structure

CAS No. : 299946-20-6

规格 是否有货
100 mg ; 询价 ;
250 mg ; 询价 ;
500 mg ; 询价 ;

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生物活性

Bax BH3 peptide (55-74), wild type is a 20-amino acid Bax BH3 peptide (Bax 1) capable of inducing apoptosis in a variety of cell line models[1].

体外研究
(In Vitro)

In addition to disrupting Bax/Bcl-2 and Bax/Bcl-XL, Bax BH3 peptide (55-74), wild type can promote cytochrome c release from isolated mitochondria. Bax BH3 belongs to the Bcl-2 protein family which consists of both pro-apoptotic and anti-apoptotic members. Bax BH3 acts to regulate apoptosis via governance of the ‘intrinsic’ pathway of cell death[1].

MCE has not independently confirmed the accuracy of these methods. They are for reference only.
分子量

2267.60

Formula

C93H163N27O34S2
CAS 号

299946-20-6
Sequence

Ser-Thr-Lys-Lys-Leu-Ser-Glu-Cys-Leu-Lys-Arg-Ile-Gly-Asp-Glu-Leu-Asp-Ser-Asn-Met
Sequence Shortening

STKKLSECLKRIGDELDSNM
运输条件

Room temperature in continental US; may vary elsewhere.
储存方式

Please store the product under the recommended conditions in the Certificate of Analysis.
Solvent Solubility
In Vitro:;

H2O

Peptide Solubility and Storage Guidelines:

1.;;Calculate the length of the peptide.

2.;;Calculate the overall charge of the entire peptide according to the following table:

; Contents Assign value
Acidic amino acid Asp (D), Glu (E), and the C-terminal -COOH. -1
Basic amino acid Arg (R), Lys (K), His (H), and the N-terminal -NH2 +1
Neutral amino acid Gly (G), Ala (A), Leu (L), Ile (I), Val (V), Cys (C), Met (M), Thr (T), Ser (S), Phe (F), Tyr (Y), Trp (W), Pro (P), Asn (N), Gln (Q) 0

3.;;Recommended solution:

Overall charge of peptide Details
Negative (lt;0) 1.;;Try to dissolve the peptide in water first.
2.;;If water fails, add NH4OH (lt;50 μL).
3.;;If the peptide still does not dissolve, add DMSO (50-100 μL) to solubilize the peptide.
Positive (gt;0) 1.;;Try to dissolve the peptide in water first.
2.;;If water fails, try dissolving the peptide in a 10%-30% acetic acid solution.
3.;;If the peptide still does not dissolve, try dissolving the peptide in a small amount of DMSO.
Zero (=0) 1.;;Try to dissolve the peptide in organic solvent (acetonitrile, methanol, etc.) first.
2.;;For very hydrophobic peptides, try dissolving the peptide in a small amount of DMSO, and then dilute the solution with water to the desired concentration.
参考文献

  • [1]. Peter E Czabotar, et al. Mutation to Bax beyond the BH3 domain disrupts interactions with pro-survival proteins and promotes apoptosis. J Biol Chem. 2011 Mar 4;286(9):7123-31.

BH3 hydrochloride

发表于由

BH3 hydrochloride;

BH3 hydrochloride 是能透过血脑屏障的多肽，通过直接激活促凋亡 Bax/Bak 或通过中和抗凋亡 Bcl-2 蛋白 (Bcl-2、Bcl-XL、Bcl-w、mcl1和A-1)来诱导细胞凋亡，通过与BH3 结构域结合。

BH3 hydrochlorideamp;;

BH3 hydrochloride Chemical Structure

规格 是否有货
100 mg ; 询价 ;
250 mg ; 询价 ;
500 mg ; 询价 ;

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生物活性

BH3 hydrochloride, a BBB penetrated peptide, provoke apoptosis either by direct activation of pro-apoptotic Bax/Bak or by neutralizing anti-apoptotic Bcl-2 proteins (Bcl-2, Bcl-XL, Bcl-w, Mcl-1 and A-1) via their BH3 domian[1][2].

分子量

2677.45

Formula

C120H183ClN36O32
Sequence

Ile-Trp-Ile-Ala-Gln-Glu-Leu-Arg-Arg-Ile-Gly-Asp-Glu-Phe-Asn-Ala-Tyr-Tyr-Ala-Arg-Arg
Sequence Shortening

IWIAQELRRIGDEFNAYYARR
运输条件

Room temperature in continental US; may vary elsewhere.
储存方式

Please store the product under the recommended conditions in the Certificate of Analysis.
参考文献

  • [1]. Guang Yang, et al. Modulating Protein-Protein Interactions In Vivo via Peptide-Lanthanide-Derived Nanoparticles for Hazard-Free Cancer Therapy. J Biomed Nanotechnol. 2019 Sep 1;15(9):1937-1947.

    [2]. Shatrunajay Shukla, et al. BH3-only Protein BIM: An Emerging Target in Chemotherapy. Eur J Cell Biol. 2017 Dec;96(8):728-738.

PUMA BH3 TFA

发表于由

PUMA BH3 TFA;

PUMA BH3 (TFA) 是一种 p53 正向凋亡调控因子 (PUMA) BH3 结构域多肽，作为 Bak 的直接激活剂，Kd 值为 26 nM。

PUMA BH3 TFAamp;;

PUMA BH3 TFA Chemical Structure

规格 是否有货
100 mg ; 询价 ;
250 mg ; 询价 ;
500 mg ; 询价 ;

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生物活性

PUMA BH3 (TFA) is a p53 upregulated modulator of apoptosis (PUMA) BH3 domain peptide, acts as a direct activator of Bak, with a Kd of 26 nM[1].

IC50 Target[1]

Bak

26 nM (Kd)

分子量

3163.32

Formula

C130H203F3N42O45S
Sequence Shortening

EEQWAREIGAQLRRMADDLNAQYER
运输条件

Room temperature in continental US; may vary elsewhere.
储存方式

Please store the product under the recommended conditions in the Certificate of Analysis.
参考文献

  • [1]. Dai H, et al. Evaluation of the BH3-only protein Puma as a direct Bak activator. J Biol Chem. 2014 Jan 3;289(1):89-99.

Bim BH3, Peptide IV TFA

发表于由

Bim BH3, Peptide IV TFA;

Bim BH3, Peptide IV TFA 是一种来自 BH3-only protein Bim 的 26 个残基肽，属于 Bcl-2 蛋白家族的促凋亡家族。

Bim BH3, Peptide IV TFAamp;;

Bim BH3, Peptide IV TFA Chemical Structure

规格 是否有货
100 mg ; 询价 ;
250 mg ; 询价 ;
500 mg ; 询价 ;

* Please select Quantity before adding items.

生物活性

Bim BH3, Peptide IV TFA is a 26-residue peptide from BH3-only protein Bim, which belongs to the pro-apoptotic group of the Bcl-2 family of proteins[1][2].

分子量

3383.67

Formula

C147H223N44F3O43S
Sequence

Asp-Met-Arg-Pro-Glu-Ile-Trp-Ile-Ala-Gln-Glu-Leu-Arg-Arg-Ile-Gly-Asp-Glu-Phe-Asn-Ala-Tyr-Tyr-Ala-Arg-Arg
Sequence Shortening

DMRPEIWIAQELRRIGDEFNAYYARR
运输条件

Room temperature in continental US; may vary elsewhere.
储存方式

Please store the product under the recommended conditions in the Certificate of Analysis.
参考文献

  • [1]. Zhu Y, et al. Constitutive association of the proapoptotic protein Bim with Bcl-2-related proteins on mitochondriain T cells. Proc Natl Acad Sci U S A. 2004 May 18;101(20):7681-6.

    [2]. Lee EF, et al. High-resolution structural characterization of a helical alpha/beta-peptide foldamer bound to the anti-apoptotic protein Bcl-xL. Angew Chem Int Ed Engl. 2009;48(24):4318-22.

PUMA BH3

发表于由

PUMA BH3;

PUMA BH3 是一种 p53 正向凋亡调控因子 (PUMA) BH3结构域多肽，作为 Bak 的直接激活剂，Kd 值为 26 nM。

PUMA BH3amp;;

PUMA BH3 Chemical Structure

规格 价格 是否有货
1 mg ￥1500 询问价格 货期
5 mg ￥3500 询问价格 货期
10 mg ￥5500 询问价格 货期

* Please select Quantity before adding items.

生物活性

PUMA BH3 is a p53 upregulated modulator of apoptosis (PUMA) BH3 domain peptide, acts as a direct activator of Bak, with a Kd of 26 nM.

IC50 Target[1]

Bak

26 nM (Kd)

体外研究
(In Vitro)

PUMA BH3 is a p53 upregulated modulator of apoptosis (PUMA) BH3 domain peptide, acts as a direct activator of Bak, with a Kd of 26 nM. PUMA BH3 binds Bak in the canonical BH3-binding groove, and activates Bak[1].

MCE has not independently confirmed the accuracy of these methods. They are for reference only.
分子量

3049.30

Formula

C128H202N42O43S
Sequence Shortening

EEQWAREIGAQLRRMADDLNAQYER
运输条件

Room temperature in continental US; may vary elsewhere.
储存方式

Please store the product under the recommended conditions in the Certificate of Analysis.
参考文献

  • [1]. Dai H, et al. Evaluation of the BH3-only protein Puma as a direct Bak activator. J Biol Chem. 2014 Jan 3;289(1):89-99.

Bak BH3

发表于由

Bak BH3;

Bak BH3 是一种多肽，源于 Bak 蛋白的 BH3 结构域，在细胞中，能够抑制 Bcl-xL 的活性。

Bak BH3amp;;

Bak BH3 Chemical Structure

规格 价格 是否有货
1 mg ￥1100 询问价格 货期
5 mg ￥3300 询问价格 货期
10 mg ￥5500 询问价格 货期

* Please select Quantity before adding items.

生物活性

Bak BH3 is derived from the BH3 domain of Bak, can antagonize the function of Bcl-xL in cells.

IC50 Target[1]

Bcl-xL

;

体外研究
(In Vitro)

Bak BH3 peptide antagonize the protective effects of microinjected Bcl-xL in α-Fas-treated HeLa cells, whereas a mutant Bak BH3 peptide that no longer binds Bcl-xL is inactive[1].

MCE has not independently confirmed the accuracy of these methods. They are for reference only.
分子量

1724.90

Formula

C72H125N25O24
Sequence

Gly-Gln-Val-Gly-Arg-Gln-Leu-Ala-Ile-Ile-Gly-Asp-Asp-Ile-Asn-Arg
Sequence Shortening

GQVGRQLAIIGDDINR
运输条件

Room temperature in continental US; may vary elsewhere.
储存方式

Please store the product under the recommended conditions in the Certificate of Analysis.
参考文献

  • [1]. Holinger EP, et al. Bak BH3 peptides antagonize Bcl-xL function and induce apoptosis through cytochrome c-independent activation of caspases. J Biol Chem. 1999 May 7;274(19):13298-304.
Cell Assay
[1]

Cells are plated in complete DMEM in 96-well tissue culture plates at 4×103/well. After 24 h, cells are washed with PBS and treated with peptides (50 μM) in SF-DMEM. Cell viability is determined by staining unfixed cells with calcein AM/ethidium homodimer, followed by microscopic analysis of cell staining and cellular morphology on a Nikon Diaphot 300 inverted microscope equipped with a fluorescence module.

MCE has not independently confirmed the accuracy of these methods. They are for reference only.
参考文献

  • [1]. Holinger EP, et al. Bak BH3 peptides antagonize Bcl-xL function and induce apoptosis through cytochrome c-independent activation of caspases. J Biol Chem. 1999 May 7;274(19):13298-304.

BMf-BH3

发表于由

BMf-BH3;

BMf-BH3 属于 Bcl-2 凋亡介质家族。BH3-only protein，Bmf 是组蛋白脱乙酰基酶 (HDAC) 抑制剂介导的增强电离辐射诱导细胞死亡的关键因子。

BMf-BH3amp;;

BMf-BH3 Chemical Structure

规格 是否有货
100 mg ; 询价 ;
250 mg ; 询价 ;
500 mg ; 询价 ;

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生物活性

BMf-BH3 belongs to the Bcl-2 apoptosis mediator family. BH3-only protein, Bmf is a key molecule for histone deacetylase (HDAC) inhibitors mediated enhancing effect on ionizing radiation-induced cell death[1][2].

分子量

3012.50

Formula

C131H214N45O35S
Sequence Shortening

LQHRAEVQIARKLQCIADQFHRLHT
运输条件

Room temperature in continental US; may vary elsewhere.
储存方式

Please store the product under the recommended conditions in the Certificate of Analysis.
参考文献

  • [1]. Zhang Y, et al. Bmf contributes to histone deacetylase inhibitor-mediated enhancing effects on apoptosis after ionizing radiation. Apoptosis. 2006 Aug;11(8):1349-57.

    [2]. Puthalakath H, et al. Bmf: a proapoptotic BH3-only protein regulated by interaction with the myosin V actin motor complex, activated by anoikis. Science. 2001 Sep 7;293(5536):1829-32.

Cali-Bio CB501488.1mg Recombinant Human B-Cell Leukemia/Lymphoma 2 -BH1，Bcl-2 -BH1 1mg

发表于由

上海金畔生物科技有限公司提供Cali-Bio CB501488.1mg Recombinant Human B-Cell Leukemia/Lymphoma 2 -BH1，Bcl-2 -BH1 1mg，欢迎访问官网了解更多产品信息和订购

品牌 货号 产品名称 规格 价格

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