AgNO3 硅胶(10%)的配制


AgNO3 硅胶(10%)的配制

AgNO3 硅胶(10%)的配制

 

5.6g AgNO3溶于21mL超纯水中

逐滴加入到50g活化硅胶中(或者11.2g AgNO3+35mL水+100g硅胶)

用铝箔纸将装AgNO3硅胶的烧瓶全部包裹住,烧瓶口用铝箔纸疏松地盖住,置于干燥烘箱中

烘箱中30℃烘5hrs(至少5hrs)

升温到60℃停留3hrs

                      ↓中间慢点升温         

升温到180℃至少12hrs

干燥器中降温后加塞密闭(保存在棕色干燥器中备用)

 

Note:如果在制作过程中发现AgNO3硅胶颜色加深或者局部变为灰黑色,应该弃去此次配制。变色原因:升温过快。

Note:装AgNO3的小烧杯要尽快用超纯水洗净,否则AgNO3会粘到烧杯上,不容易洗干净。

Na2SO4: 660℃下马福炉中烘至少6 hrs(优级纯)

注意:提取筒洗过后要在马福炉里420℃烘一段时间

 

纯化注意事项:

1.所有纯化柱均采用干法装制;

2装好填料后轻拍柱子至填料表层平整;

3一旦加上洗脱液后,则不要再拍动柱子;

4一定量的hexane预淋洗进行稳定,并且除去部分污染杂质;

5预淋洗时流速控制在2滴/S;

6填料若出现断层则不能再使用;

7上柱样品后,用1mL正己烷清洗烧瓶;

8要等上一组分液面约1mm时再上样;

9若是做流出曲线,对体积要求就会严格一些,接流出物的量筒用铝箔包上,防止溶剂挥发。

 

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加拿大Silicycle硅胶填料SiliaBond Silver Nitrate (AgNO3), 40 – 63 µm, 60 Å (R23530B)

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SiliaBond Silver Nitrate (AgNO3), 40 – 63 µm, 60 Å (R23530B)

SiliaBond Silver Nitrate (AgNO3), 40 - 63 µm, 60 Å (R23530B)

Prices do not include applicable taxes, customs, duties and/or shipping.

Product Number Quantity  
R23530B-10G 10 g

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R23530B-25G 25 g

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R23530B-50G 50 g

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R23530B-100G 100 g

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R23530B-250G 250 g

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R23530B-500G 500 g

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R23530B-1KG 1 kg

SiliaBond Silver Nitrate (AgNO3), 40 - 63 µm, 60 Å (R23530B)   Loading…  

Details

Chromatography with silver nitrate is a well known and established methodology for the separation of non polar compounds with similar polarities. Generally, with a mixture of unsaturated compounds such as alkenes, lipids, steroids, terpenes, etc, standard separation procedures lack in efficiency. However, by using SiliaBond® Silver Nitrate for your column chromatography along with SiliaPlate Ag for your TLC, you will have the necessary tools to circumvent the problem. The theory behind this separation is based on the fact that silver ions can complex with unsaturated compounds (π bond). This kind of chromatography with silver nitrate impregnated silica gel is very easy to perform and is well documented.1-4

PRODUCT SPECIFICATIONS

MSDS-R23530B

RELATED PUBLICATIONS

  1. Tetrahedron, 57 (2001) 425
  2. J. Chromato. A, 715 (1995) 372
  3. J. Chromato. A, 38 (1968) 535
  4. Microchem. J., 9 (1965) 227

Additional Information

Particle Shape Irregular
Array Silver Nitrate (AgNO3)
Family / Formats SiliaBond® (Functionalized Silicas)
Particle Size 40 – 63 µm / 230 – 400 mesh
Pore Size 60 Å
Storage Condition Keep dry in the dark