标签归档：货期

Speract(Synonyms: 精子活化肽)

发表于由

Speract;(Synonyms: 精子活化肽)

Speract 是一种调节精子活力的海胆蛋肽，也刺激精子线粒体代谢。

Speractamp;;(Synonyms: 精子活化肽)

Speract Chemical Structure

CAS No. : 76901-59-2

规格 价格 是否有货
1 mg ￥700 询问价格 货期
5 mg ￥2800 询问价格 货期
10 mg ￥4900 询问价格 货期

* Please select Quantity before adding items.

生物活性

Speract, a sea urchin egg peptide that regulates sperm motility, also stimulates sperm mitochondrial metabolism.

体外研究
(In Vitro)

Speract is a decapeptide from the outer jelly layer of theStrongylocentrotus purpuratus egg that upon binding to its receptor in the sperm, stimulates sperm motility, respiration and ion fluxes, among other physiological events. Speract increases NADH levels and depolarizes the sea urchin sperm mitochondrion[1].

MCE has not independently confirmed the accuracy of these methods. They are for reference only.
分子量

891.92

Formula

C38H57N11O14
CAS 号

76901-59-2
Sequence

Gly-Phe-Asp-Leu-Asn-Gly-Gly-Gly-Val-Gly
Sequence Shortening

GFDLNGGGVG
中文名称

精子活化肽
运输条件

Room temperature in continental US; may vary elsewhere.
储存方式

Please store the product under the recommended conditions in the Certificate of Analysis.
参考文献

  • [1]. García-Rincón J, et al. Speract, a sea urchin egg peptide that regulates sperm motility, also stimulates sperm mitochondrial metabolism. Biochim Biophys Acta. 2016 Apr;1857(4):415-26.

Exendin-3

发表于由

Exendin-3;

Exendin-3是从蜥蜴 Heloderma horridurn 毒液分离的生物活性肽。

Exendin-3amp;;

Exendin-3 Chemical Structure

CAS No. : 130357-25-4

规格 价格 是否有货
500 μg ￥2200 询问价格 货期
1 mg ￥3200 询问价格 货期
5 mg ￥9900 询问价格 货期

* Please select Quantity before adding items.

生物活性

Exendin-3 is a biologically active peptides isolated from venoms of the Gila monster lizards, Heloderma horridurn.

IC50 Target

VIP receptor, putative exendin receptor[1]
体外研究
(In Vitro)

Exendin-3 interacts with at least two receptors on guinea pig pancreatic acini; at high concentrations (>100 nM) the peptide interacts with VIP receptors, thereby causing a large increase in cAMP and stimulating amylase release; at lower concentrations (0.1-3 nM) the peptide interacts with a putative exendin receptor, thereby causing a smaller increase in cAMP of undetermined function[1].

MCE has not independently confirmed the accuracy of these methods. They are for reference only.
分子量

4202.57

Formula

C184H282N50O61S
CAS 号

130357-25-4
Sequence

His-Ser-Asp-Gly-Thr-Phe-Thr-Ser-Asp-Leu-Ser-Lys-Gln-Met-Glu-Glu-Glu-Ala-Val-Arg-Leu-Phe-Ile-Glu-Trp-Leu-Lys-Asn-Gly-Gly-Pro-Ser-Ser-Gly-Ala-Pro-Pro-Pro-Ser-NH2
Sequence Shortening

HSDGTFTSDLSKQMEEEAVRLFIEWLKNGGPSSGAPPPS-NH2
运输条件

Room temperature in continental US; may vary elsewhere.
储存方式

Please store the product under the recommended conditions in the Certificate of Analysis.
Solvent Solubility
In Vitro:;

H2O

Peptide Solubility and Storage Guidelines:

1.;;Calculate the length of the peptide.

2.;;Calculate the overall charge of the entire peptide according to the following table:

; Contents Assign value
Acidic amino acid Asp (D), Glu (E), and the C-terminal -COOH. -1
Basic amino acid Arg (R), Lys (K), His (H), and the N-terminal -NH2 +1
Neutral amino acid Gly (G), Ala (A), Leu (L), Ile (I), Val (V), Cys (C), Met (M), Thr (T), Ser (S), Phe (F), Tyr (Y), Trp (W), Pro (P), Asn (N), Gln (Q) 0

3.;;Recommended solution:

Overall charge of peptide Details
Negative (lt;0) 1.;;Try to dissolve the peptide in water first.
2.;;If water fails, add NH4OH (lt;50 μL).
3.;;If the peptide still does not dissolve, add DMSO (50-100 μL) to solubilize the peptide.
Positive (gt;0) 1.;;Try to dissolve the peptide in water first.
2.;;If water fails, try dissolving the peptide in a 10%-30% acetic acid solution.
3.;;If the peptide still does not dissolve, try dissolving the peptide in a small amount of DMSO.
Zero (=0) 1.;;Try to dissolve the peptide in organic solvent (acetonitrile, methanol, etc.) first.
2.;;For very hydrophobic peptides, try dissolving the peptide in a small amount of DMSO, and then dilute the solution with water to the desired concentration.
参考文献

  • [1]. Raufman JP, et al. Exendin-3, a novel peptide from Heloderma horridum venom, interacts with vasoactive intestinal peptide receptors and a newly described receptor on dispersed acini from guinea pig pancreas. Description of exendin-3(9-39) amide, a specific exendin receptor antagonist. J Biol Chem. 1991 Feb 15;266(5):2897-902.
Kinase Assay
[1]

Effect of increasing concentrations of two VIP receptor antagonists on the increase in amylase release are observed with VIP, secretin, or exendin-3. Male Hartley guinea pigs Acini were incubated with VIP (1 nM), secretin (1 μM), or Exendin-3 (1 μM) for 30 min at 37°C, alone or in combination with indicated concentrations of [AcTyr, D-Phe]GRF 1-29 amide (solid symbols) or [4-Cl-D-Phe,Leu] VIP (open symbols)[1].

MCE has not independently confirmed the accuracy of these methods. They are for reference only.
参考文献

  • [1]. Raufman JP, et al. Exendin-3, a novel peptide from Heloderma horridum venom, interacts with vasoactive intestinal peptide receptors and a newly described receptor on dispersed acini from guinea pig pancreas. Description of exendin-3(9-39) amide, a specific exendin receptor antagonist. J Biol Chem. 1991 Feb 15;266(5):2897-902.

TAT 2-4

发表于由

TAT 2-4;

TAT 2-4 是一种多肽，来源于 HIV-1 反式激活因子 (Tat)。

TAT 2-4amp;;

TAT 2-4 Chemical Structure

CAS No. : 1159916-66-1

规格 价格 是否有货
1 mg ￥900 询问价格 货期
5 mg ￥2200 询问价格 货期
10 mg ￥3500 询问价格 货期

* Please select Quantity before adding items.

生物活性

TAT 2-4 is a peptide derived from HIV-1 transactivator of transcription (Tat) protein.

分子量

3215.74

Formula

C132H240N66O29
CAS 号

1159916-66-1
Sequence

Tyr-Gly-Arg-Lys-Lys-Arg-Arg-Gln-Arg-Arg-Arg-Gly-Tyr-Gly-Arg-Lys-Lys-Arg-Arg-Gln-Arg-Arg-Arg-Gly
Sequence Shortening

YGRKKRRQRRRGYGRKKRRQRRRG
运输条件

Room temperature in continental US; may vary elsewhere.
储存方式

Please store the product under the recommended conditions in the Certificate of Analysis.
Solvent Solubility
In Vitro:;

H2O

Peptide Solubility and Storage Guidelines:

1.;;Calculate the length of the peptide.

2.;;Calculate the overall charge of the entire peptide according to the following table:

; Contents Assign value
Acidic amino acid Asp (D), Glu (E), and the C-terminal -COOH. -1
Basic amino acid Arg (R), Lys (K), His (H), and the N-terminal -NH2 +1
Neutral amino acid Gly (G), Ala (A), Leu (L), Ile (I), Val (V), Cys (C), Met (M), Thr (T), Ser (S), Phe (F), Tyr (Y), Trp (W), Pro (P), Asn (N), Gln (Q) 0

3.;;Recommended solution:

Overall charge of peptide Details
Negative (lt;0) 1.;;Try to dissolve the peptide in water first.
2.;;If water fails, add NH4OH (lt;50 μL).
3.;;If the peptide still does not dissolve, add DMSO (50-100 μL) to solubilize the peptide.
Positive (gt;0) 1.;;Try to dissolve the peptide in water first.
2.;;If water fails, try dissolving the peptide in a 10%-30% acetic acid solution.
3.;;If the peptide still does not dissolve, try dissolving the peptide in a small amount of DMSO.
Zero (=0) 1.;;Try to dissolve the peptide in organic solvent (acetonitrile, methanol, etc.) first.
2.;;For very hydrophobic peptides, try dissolving the peptide in a small amount of DMSO, and then dilute the solution with water to the desired concentration.

Kassinin(Synonyms: 肛褶蛙肽)

发表于由

Kassinin;(Synonyms: 肛褶蛙肽)

Kassinin 是一种衍生自 Kassina 蛙的肽。Kassinin属于速激肽家族的神经肽。Kassinin分泌作为防御信号，并参与神经肽信号传导。

Kassininamp;;(Synonyms: 肛褶蛙肽)

Kassinin Chemical Structure

CAS No. : 63968-82-1

规格 价格 是否有货
1 mg ￥770 询问价格 货期
5 mg ￥2300 询问价格 货期
10 mg ￥3850 询问价格 货期
25 mg ￥8200 询问价格 货期

* Please select Quantity before adding items.

生物活性

Kassinin is a peptide derived from the Kassina frog. It belongs to tachykinin family of neuropeptides. It is secreted as a defense response, and is involved in neuropeptide signalling.

体外研究
(In Vitro)

Amino acid composition and sequence of Kassinin, a tachykinin dodecapeptide from the skin of the African frog Kassina senegalensis. Methanol extracts of the skin of the African amphibian Kassina senegalensis contain a dodecapeptide, Kassinin, belonging to the family of tachykinins or physalaemin-like peptides. Kassinin, like all other natural tachykinins, possesses the characteristic C-terminal tripeptide Gly-Leu-Met-NH2 and a phenylalanine residue in position 5 from the C-terminus. The amino acid sequence in the N-moiety of the molecule differs sharply from that of the other tachykinins[1].

MCE has not independently confirmed the accuracy of these methods. They are for reference only.
分子量

1334.54

Formula

C59H95N15O18S
CAS 号

63968-82-1
Sequence

Asp-Val-Pro-Lys-Ser-Asp-Gln-Phe-Val-Gly-Leu-Met-NH2
Sequence Shortening

DVPKSDQFVGLM-NH2
中文名称

肛褶蛙肽
运输条件

Room temperature in continental US; may vary elsewhere.
储存方式

Please store the product under the recommended conditions in the Certificate of Analysis.
参考文献

  • [1]. Anastasi A, et al. Amino acid composition and sequence of kassinin, a tachykinin dodecapeptide from the skin of the African frog Kassina senegalensis. Experientia. 1977 Jul 15;33(7):857-8.

Ceratotoxin A

发表于由

Ceratotoxin A;

Ceratotoxin A 是由 29 个氨基酸组成的多肽，从副腺分泌液中分离到，具有抗细菌 (anti-bacterial) 的作用。

Ceratotoxin Aamp;;

Ceratotoxin A Chemical Structure

CAS No. : 150671-04-8

规格 价格 是否有货
1 mg ￥1100 询问价格 货期
5 mg ￥3500 询问价格 货期
10 mg ￥5500 询问价格 货期

* Please select Quantity before adding items.

生物活性

Ceratotoxin A, a 29-residue peptide isolated from the accessory gland secretion fluid, with strong anti-bacterial activity.

IC50 Target

Bacterial[1]
体外研究
(In Vitro)

Ceratotoxin A is a 29-residue peptide isolated from the accessory gland secretion fluid, shows anti-E.coli activity, and is heat stable[1]. Ceratotoxin A is effective against Escherichia coli ATCC 23739, Pseudomonas aeruginos ATCC 27853, and Bacillus subtilis ATCC 6633 with minimal inhibitory concentration (MIC) of 7, 7, and 3.5 µM[2].

MCE has not independently confirmed the accuracy of these methods. They are for reference only.
分子量

2868.59

Formula

C135H243N35O32
CAS 号

150671-04-8
Sequence

Ser-Ile-Gly-Ser-Ala-Leu-Lys-Lys-Ala-Leu-Pro-Val-Ala-Lys-Lys-Ile-Gly-Lys-Ile-Ala-Leu-Pro-Ile-Ala-Lys-Ala-Ala-Leu-Pro
Sequence Shortening

SIGSALKKALPVAKKIGKIALPIAKAALP
运输条件

Room temperature in continental US; may vary elsewhere.
储存方式

Please store the product under the recommended conditions in the Certificate of Analysis.
参考文献

  • [1]. Marchini D, et al. Purification and primary structure of ceratotoxin A and B, two antibacterial peptides from the female reproductive accessory glands of the medfly Ceratitis capitata (Insecta:Diptera). Insect Biochem Mol Biol. 1993 Jul;23(5):591-8.

    [2]. Marri L, et al. The novel antibacterial peptide ceratotoxin A alters permeability of the inner and outer membrane of Escherichia coli K-12. Curr Microbiol. 1996 Jul;33(1):40-3.

Somatostatin-25

发表于由

Somatostatin-25;

Somatostatin-25 是一种内源性神经肽激素，能够抑制生长激素的分泌。

Somatostatin-25amp;;

Somatostatin-25 Chemical Structure

CAS No. : 76461-17-1

规格 价格 是否有货
500 μg ￥2200 询问价格 货期
1 mg ￥3500 询问价格 货期
5 mg ￥9900 询问价格 货期

* Please select Quantity before adding items.

生物活性

Somatostatin-25 is a endogenous neuropeptide hormone that shows inhibitory activity against secretion of growth hormone.

体外研究
(In Vitro)

Somatostatin-25 (0.1 pM-1 nM) significantly inhibits secretion of growth hormone in the in vitro system, and is 3-14 times more effective than the tetradecapeptide. Somatostatin-25 (0.1 nM-1pM) also inhibits secretion of growth hormone induced by the addition of prostaglandin 2 (PGE2). However, Somatostatin-25 shows no effect on the secretion of prolactin[1].

MCE has not independently confirmed the accuracy of these methods. They are for reference only.
分子量

2876.30

Formula

C127H191N37O34S3
CAS 号

76461-17-1
Sequence

Ser-Asn-Pro-Ala-Met-Ala-Pro-Arg-Glu-Arg-Lys-Ala-Gly-Cys-Lys-Asn-Phe-Phe-Trp-Lys-Thr-Phe-Thr-Ser-Cys (Disulfide bridge: Cys14-Cys25)
Sequence Shortening

SNPAMAPRERKAGCKNFFWKTFTSC (Disulfide bridge: Cys14-Cys25)
运输条件

Room temperature in continental US; may vary elsewhere.
储存方式

Please store the product under the recommended conditions in the Certificate of Analysis.
Solvent Solubility
In Vitro:;

H2O

Peptide Solubility and Storage Guidelines:

1.;;Calculate the length of the peptide.

2.;;Calculate the overall charge of the entire peptide according to the following table:

; Contents Assign value
Acidic amino acid Asp (D), Glu (E), and the C-terminal -COOH. -1
Basic amino acid Arg (R), Lys (K), His (H), and the N-terminal -NH2 +1
Neutral amino acid Gly (G), Ala (A), Leu (L), Ile (I), Val (V), Cys (C), Met (M), Thr (T), Ser (S), Phe (F), Tyr (Y), Trp (W), Pro (P), Asn (N), Gln (Q) 0

3.;;Recommended solution:

Overall charge of peptide Details
Negative (lt;0) 1.;;Try to dissolve the peptide in water first.
2.;;If water fails, add NH4OH (lt;50 μL).
3.;;If the peptide still does not dissolve, add DMSO (50-100 μL) to solubilize the peptide.
Positive (gt;0) 1.;;Try to dissolve the peptide in water first.
2.;;If water fails, try dissolving the peptide in a 10%-30% acetic acid solution.
3.;;If the peptide still does not dissolve, try dissolving the peptide in a small amount of DMSO.
Zero (=0) 1.;;Try to dissolve the peptide in organic solvent (acetonitrile, methanol, etc.) first.
2.;;For very hydrophobic peptides, try dissolving the peptide in a small amount of DMSO, and then dilute the solution with water to the desired concentration.
参考文献

  • [1]. Brazeau P, et al. High biological activity of the synthetic replicates of somatostatin-28 and somatostatin-25. Regul Pept. 1981 Jan;1(4):255-64.

CEF4

发表于由

CEF4;

CEF4 是一种多肽，它与甲型流感病毒核衣壳蛋白的第 342-351 位氨基酸序列相对应。

CEF4amp;;

CEF4 Chemical Structure

CAS No. : 199727-68-9

规格 价格 是否有货
1 mg ￥800 询问价格 货期
5 mg ￥3200 询问价格 货期
10 mg ￥5600 询问价格 货期

* Please select Quantity before adding items.

生物活性

CEF4 is a peptide that corresponds to aa 342-351 of the influenza A virus nucleocapsid protein.

分子量

1148.40

Formula

C53H93N15O13
CAS 号

199727-68-9
Sequence

Arg-Val-Leu-Ser-Phe-Ile-Lys-Gly-Thr-Lys
Sequence Shortening

RVLSFIKGTK
运输条件

Room temperature in continental US; may vary elsewhere.
储存方式

Please store the product under the recommended conditions in the Certificate of Analysis.
Solvent Solubility
In Vitro:;

H2O

Peptide Solubility and Storage Guidelines:

1.;;Calculate the length of the peptide.

2.;;Calculate the overall charge of the entire peptide according to the following table:

; Contents Assign value
Acidic amino acid Asp (D), Glu (E), and the C-terminal -COOH. -1
Basic amino acid Arg (R), Lys (K), His (H), and the N-terminal -NH2 +1
Neutral amino acid Gly (G), Ala (A), Leu (L), Ile (I), Val (V), Cys (C), Met (M), Thr (T), Ser (S), Phe (F), Tyr (Y), Trp (W), Pro (P), Asn (N), Gln (Q) 0

3.;;Recommended solution:

Overall charge of peptide Details
Negative (lt;0) 1.;;Try to dissolve the peptide in water first.
2.;;If water fails, add NH4OH (lt;50 μL).
3.;;If the peptide still does not dissolve, add DMSO (50-100 μL) to solubilize the peptide.
Positive (gt;0) 1.;;Try to dissolve the peptide in water first.
2.;;If water fails, try dissolving the peptide in a 10%-30% acetic acid solution.
3.;;If the peptide still does not dissolve, try dissolving the peptide in a small amount of DMSO.
Zero (=0) 1.;;Try to dissolve the peptide in organic solvent (acetonitrile, methanol, etc.) first.
2.;;For very hydrophobic peptides, try dissolving the peptide in a small amount of DMSO, and then dilute the solution with water to the desired concentration.

Substance P (1-9)

发表于由

Substance P (1-9);

Substance P (1-9) 是一种九肽，能够降低豚鼠回肠和膀胱中 substance P 的非活化程度。

Substance P (1-9)amp;;

Substance P (1-9) Chemical Structure

CAS No. : 57468-17-4

规格 价格 是否有货
1 mg ￥600 询问价格 货期
5 mg ￥1800 询问价格 货期
10 mg ￥2800 询问价格 货期

* Please select Quantity before adding items.

生物活性

Substance P (1-9) is nonapeptide, which decreases the inactivation of substance P by the guinea-pig ileum and urinary bladder.

体外研究
(In Vitro)

Substance P (1-9) is a nonapeptide, which decreases the inactivation of substance P by the guinea-pig ileum and urinary bladder, but with no significant effects on bradykinin or SP-(6-11)[1].

MCE has not independently confirmed the accuracy of these methods. They are for reference only.
分子量

1104.26

Formula

C52H77N15O12
CAS 号

57468-17-4
Sequence

Arg-Pro-Lys-Pro-Gln-Gln-Phe-Phe-Gly
Sequence Shortening

RPKPQQFFG
运输条件

Room temperature in continental US; may vary elsewhere.
储存方式

Please store the product under the recommended conditions in the Certificate of Analysis.
参考文献

  • [1]. Growcott JW, et al. Effects of substance P-(1-9) nonapeptide amide on inactivation of substance P in vitro. Eur J Pharmacol. 1982 Oct 15;84(1-2):107-9.

CEF6

发表于由

CEF6;

CEF6 是由 9 个氨基酸构成的多肽，与流感病毒 H1N1 的核衣壳蛋白的第 418-426 位氨基酸序列相对应。

CEF6amp;;

CEF6 Chemical Structure

CAS No. : 913545-15-0

规格 价格 是否有货
1 mg ￥600 询问价格 货期
5 mg ￥1800 询问价格 货期
10 mg ￥2800 询问价格 货期

* Please select Quantity before adding items.

生物活性

CEF6 is a 9-aa-long peptide corresponding to aa 418-426 of the influenza A virus (H1N1) nucleocapsid protein.

分子量

1051.26

Formula

C48H78N10O14S1
CAS 号

913545-15-0
Sequence

Leu-Pro-Phe-Asp-Lys-Thr-Thr-Val-Met
Sequence Shortening

LPFDKTTVM
运输条件

Room temperature in continental US; may vary elsewhere.
储存方式

Please store the product under the recommended conditions in the Certificate of Analysis.
参考文献

  • [1]. Yu K, et al. shRNA?mediated NP knockdown inhibits the apoptosis of cardiomyocytes induced by H1N1pdm2009 influenza virus. Mol Med Rep. 2017 Aug;16(2):1376-1382.

Cerebellin

发表于由

Cerebellin;

Cerebellin是广泛分布于中枢神经系统的神经调节肽。

Cerebellinamp;;

Cerebellin Chemical Structure

CAS No. : 94071-26-8

规格 价格 是否有货
1 mg ￥2200 询问价格 货期
5 mg ￥6600 询问价格 货期
10 mg ￥9900 询问价格 货期

* Please select Quantity before adding items.

生物活性

Cerebellin is a neuromodulatory peptide widely distributed in the central nervous system.

体外研究
(In Vitro)

Cerebellin concentration-dependently (from 1 to 100 nM) increases norepinephrine (but not epinephrine) and cyclic-AMP production by adrenomedullary tissue in vitro[1].

MCE has not independently confirmed the accuracy of these methods. They are for reference only.
体内研究
(In Vivo)

Cerebellin potently stimulates norepinephrine release by rat adrenal medulla, acting through adenylate-cyclase/PKA-coupled receptors, and enhances adrenocortical steroid secretion in vivo (i.e. when the integrity of adrenal gland is preserved) through an indirect paracrine mechanism involving the release of medullary catecholamines[1]. Cerebellin reduces plasma insulin levels in rats after 1 and 2 h. Cerebellin decreases insulin secretion from isolated rat pancreatic islets at high (11 mM), but not at low (3.33 mM) glucose concentration. Cerebellin inhibits stimulated insulin secretion from clonal rat insulinoma (INS-1) cells, reduces forskolin-induced production of cAMP and intracellular calcium concentration[2].

MCE has not independently confirmed the accuracy of these methods. They are for reference only.
分子量

1632.78

Formula

C69H113N23O23
CAS 号

94071-26-8
Sequence

Ser-Gly-Ser-Ala-Lys-Val-Ala-Phe-Ser-Ala-Ile-Arg-Ser-Thr-Asn-His
Sequence Shortening

SGSAKVAFSAIRSTNH
运输条件

Room temperature in continental US; may vary elsewhere.
储存方式

Please store the product under the recommended conditions in the Certificate of Analysis.
Solvent Solubility
In Vitro:;

H2O

Peptide Solubility and Storage Guidelines:

1.;;Calculate the length of the peptide.

2.;;Calculate the overall charge of the entire peptide according to the following table:

; Contents Assign value
Acidic amino acid Asp (D), Glu (E), and the C-terminal -COOH. -1
Basic amino acid Arg (R), Lys (K), His (H), and the N-terminal -NH2 +1
Neutral amino acid Gly (G), Ala (A), Leu (L), Ile (I), Val (V), Cys (C), Met (M), Thr (T), Ser (S), Phe (F), Tyr (Y), Trp (W), Pro (P), Asn (N), Gln (Q) 0

3.;;Recommended solution:

Overall charge of peptide Details
Negative (lt;0) 1.;;Try to dissolve the peptide in water first.
2.;;If water fails, add NH4OH (lt;50 μL).
3.;;If the peptide still does not dissolve, add DMSO (50-100 μL) to solubilize the peptide.
Positive (gt;0) 1.;;Try to dissolve the peptide in water first.
2.;;If water fails, try dissolving the peptide in a 10%-30% acetic acid solution.
3.;;If the peptide still does not dissolve, try dissolving the peptide in a small amount of DMSO.
Zero (=0) 1.;;Try to dissolve the peptide in organic solvent (acetonitrile, methanol, etc.) first.
2.;;For very hydrophobic peptides, try dissolving the peptide in a small amount of DMSO, and then dilute the solution with water to the desired concentration.
参考文献

  • [1]. Albertin G, et al. Cerebellin stimulates the secretory activity of the rat adrenal gland: in vitro and in vivo studies. Neuropeptides. 2000 Feb;34(1):7-11.

    [2]. Strowski MZ, et al. Insulinostatic activity of cerebellin–evidence from in vivo and in vitro studies in rats. Regul Pept. 2009 Oct 9;157(1-3):19-24.
Kinase Assay
[1]

Some adrenomedullary samples are incubated with 10 μM H89, U-73122 or calphostin-C alone or in the presence of 0.1 μM cerebellin. When cyclic-AMP production is assayed, 100 μM 3-isobutyl-1-methylxanthine is added to prevent cyclic-AMP metabolism by phosphodiesterases. Incubation is carried out for 60 min (aldosterone or corticosterone production), 30 min (catecholamine production) or 10 min (cyclic-AMP production) in a shaking bath at 37°C in an atmosphere of 95% air -5% CO2. At the end of the experiments, the incubation tubes are centrifuged at 4°C, and media are collected and kept frozen at -80°C[1].

MCE has not independently confirmed the accuracy of these methods. They are for reference only.
Animal Administration
[2]

Rats[2]

Adult female Wistar rats (180–190 g body weight, 8–12 weeks of age) are injected daily with 0.2 mL of 0.9% NaCl (s.c.) for 14 days. On day 15, animals receive s.c. injections of 0.2 mL of 0.9% NaCl (control groups), while experimental groups are treated with 0.5 or 1.5 nmol/100 g body weight Cerebellin0.5 or 1.5 nmol/100 g body weight Cerebellin. Rats are decapitated 60 or 120 min after injection. Blood is collected into ice-cold tubes containing EDTA (1 mg/mL) and analyzed for the concentration of glucose. Plasma is separated and stored at −80 °C for the determination of the insulin and glucagon concentration[2].

MCE has not independently confirmed the accuracy of these methods. They are for reference only.
参考文献

  • [1]. Albertin G, et al. Cerebellin stimulates the secretory activity of the rat adrenal gland: in vitro and in vivo studies. Neuropeptides. 2000 Feb;34(1):7-11.

    [2]. Strowski MZ, et al. Insulinostatic activity of cerebellin–evidence from in vivo and in vitro studies in rats. Regul Pept. 2009 Oct 9;157(1-3):19-24.