标签归档：蛙皮素

Magainin 2(Synonyms: 蛙皮素 2 Magainin II)

发表于由

Magainin 2;(Synonyms: 蛙皮素 2; Magainin II) 纯度: 99.34%

Magainin 2 (Magainin II) 是一种抗菌肽 (AMP)，从非洲爪蟾皮肤中分离得到。Magainin 2 显示抗生素活性对许多革兰氏阴性和革兰氏阳性细菌。Magainin 2 对原生动物也有活性。Magainin 2 通过优先与细菌膜中丰富的阴离子磷脂相互作用而发挥细胞毒性作用。

Magainin 2amp;;(Synonyms: 蛙皮素 2; Magainin II)

Magainin 2 Chemical Structure

CAS No. : 108433-95-0

规格 价格 是否有货 数量
500 μg ￥900 In-stock
1 mg ￥1600 In-stock
5 mg ￥6400 In-stock
10 mg ￥9900 In-stock
50 mg ; 询价 ;
100 mg ; 询价 ;

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Magainin 2 相关产品

bull;相关化合物库:

  • Natural Product Library Plus
  • Bioactive Compound Library Plus
  • Peptide Library

生物活性

Magainin 2 (Magainin II) is an antimicrobial peptide (AMP) isolated from the skin of the African clawed frog Xenopus laevis. Magainin 2 displays antibiotic activity against numerous gram-negative and gram-positive bacteria. Magainin 2 also is active against protozoa[1]. Magainin 2 exerts its cytotoxicity effects by preferential interactions with anionic phospholipids abundant in bacterial membranes[2].

体外研究
(In Vitro)

Magainin 2 exhibits bactericidal effects and induces morphological changes in Escherichia coli regarding early apoptosis. Magainin 2 induces the expression of a bacterial protein with affinity for the caspase substrate and effects the expression of RecA as a caspase-like protein[1]. Magainin 2 kill bacteria by permeabilizing the cell membranes without exhibiting significant toxicity against mammalian cells. The main target of the peptide is considered to be the lipid matrix of the membranes. Application of 10 μg /mL magainin 2 to Paramecium caudatum, a protozoan, in pond water caused an osmotic swelling of the cell and a subsequent cell burst, suggesting that the peptide could perturb membrane functions responsible for osmotic balance[2]. Magainin 2 permeabilizes bacterial and mammalian membranes in significantly different ways. The peptide forms pores with a diameter of about 2.8 nm (less than 6.6 nm) in B. megaterium, and translocates into the cytosol. In contrast, the peptide significantly perturbes the membrane of CHO-K1 cells, permitting the entry of a large molecule (larger than 23 nm) into the cytosol, accompanied by membrane budding and lipid flip-flop, mainly accumulating in mitochondria and nuclei[3].

MCE has not independently confirmed the accuracy of these methods. They are for reference only.
分子量

2466.90

Formula

C114H180N30O29S
CAS 号

108433-95-0
Sequence

Gly-Ile-Gly-Lys-Phe-Leu-His-Ser-Ala-Lys-Lys-Phe-Gly-Lys-Ala-Phe-Val-Gly-Glu-Ile-Met-Asn-Ser
Sequence Shortening

GIGKFLHSAKKFGKAFVGEIMNS
中文名称

蛙皮素 2
运输条件

Room temperature in continental US; may vary elsewhere.
储存方式

Protect from light

Powder -80deg;C 2 years
-20deg;C 1 year

*In solvent : -80deg;C, 6 months; -20deg;C, 1 month (protect from light)
参考文献

  • [1]. M Zasloff, et al. Magainins, a class of antimicrobial peptides from Xenopus skin: isolation, characterization of two active forms, and partial cDNA sequence of a precursor. Proc Natl Acad Sci U S A. 1987 Aug;84(15):5449-53.

    [2]. K Matsuzaki, et al. Magainins as paradigm for the mode of action of pore forming polypeptides. Biochim Biophys Acta. 1998 Nov 10;1376(3):391-400.
Cell Assay
[1]

Escherichia coli cells are incubated with magainin 2 (50 μg /mL). The cultures are acquired after incubation for 0, 2, 4, 6, and 8 h, respectively, and spread onto LB agar plates, and then the colony-forming units are counted after incubation for 24 h at 37°C. The percentage survival is determined relative to the control treatment[1].

MCE has not independently confirmed the accuracy of these methods. They are for reference only.
参考文献

  • [1]. M Zasloff, et al. Magainins, a class of antimicrobial peptides from Xenopus skin: isolation, characterization of two active forms, and partial cDNA sequence of a precursor. Proc Natl Acad Sci U S A. 1987 Aug;84(15):5449-53.

    [2]. K Matsuzaki, et al. Magainins as paradigm for the mode of action of pore forming polypeptides. Biochim Biophys Acta. 1998 Nov 10;1376(3):391-400.