标签归档：藏红花

Transcrocetin(Synonyms: 藏红花酸; trans-Crocetin)

发表于由

上海金畔生物科技有限公司提供天然产物萜类及其苷类Terpenoids and Glycosides。

Transcrocetin (Synonyms: 藏红花酸; trans-Crocetin) 纯度: 98.04%

Transcrocetin 从藏红花 (Crocus sativus L.) 中提取的，是一种可透过血脑屏障的，高亲和力的 NMDA 受体拮抗剂。

Transcrocetin(Synonyms: 藏红花酸; trans-Crocetin)

Transcrocetin Chemical Structure

CAS No. : 27876-94-4

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Transcrocetin 相关产品

•相关化合物库:

  • Natural Product Library Plus
  • Drug Repurposing Compound Library Plus
  • Clinical Compound Library Plus
  • Bioactive Compound Library Plus

生物活性

Transcrocetin (trans-Crocetin), extracted from saffron (Crocus sativus L.), acts as an NMDA receptor antagonist with high affinity[1]. Transcrocetin (trans-Crocetin) is capable of crossing the blood-brain barrier and reach the central nervous system (CNS)[2].

IC50 & Target

Human Endogenous Metabolite

 

体外研究
(In Vitro)

Transcrocetin (trans-Crocetin), a saffron metabolite originating from the crocin apocarotenoids, has been shown to exert strong NMDA receptor affinity and is thought to be responsible for the CNS activity of saffron.To ensure unchanged viability of Caco-2 cells throughout the transport experiments, cellular mitochondrial dehydrogenase activity of Caco-2 cells is measured by MTT assay after a 24 h incubation period with the test compounds: Hydroalcoholic saffron extract saffron extract (SE, 0.5-1 mg/mL) and crocin-1 (250-1000 µM) reveal no negative significant changes in cellular viability. Transcrocetin at 10 µM level does not change viability while higher concentrations (40-160 µM) reduces significantly cellular viability[1].

Shanghai Jinpan Biotech Co Ltd has not independently confirmed the accuracy of these methods. They are for reference only.
Clinical Trial

分子量

328.40

Formula

C20H24O4
CAS 号

27876-94-4
中文名称

藏红花酸；西红花酸；番红花酸
运输条件

Room temperature in continental US; may vary elsewhere.
储存方式
Powder -20°C 3 years
4°C 2 years

*该产品在溶液状态不稳定，建议您现用现配，即刻使用。
溶解性数据
In Vitro: 

DMSO : 1 mg/mL (3.05 mM; Need ultrasonic and warming)

配制储备液
浓度 溶剂体积 质量 1 mg 5 mg 10 mg
1 mM 3.0451 mL 15.2253 mL 30.4507 mL
5 mM — — —
10 mM — — —

*

请根据产品在不同溶剂中的溶解度，选择合适的溶剂配制储备液；该产品在溶液状态不稳定，建议您现用现配，即刻使用。
参考文献

  • [1]. Lautenschläger M, et al. Intestinal formation of trans-Crocetin from saffron extract (Crocus sativus L.) and in vitro permeation through intestinal and blood brain barrier. Phytomedicine. 2015 Jan 15;22(1):36-44.

    [2]. José Bagur M, et al. Saffron: An Old Medicinal Plant and a Potential Novel Functional Food. Molecules. 2017 Dec 23;23(1). pii: E30.
Cell Assay
[1]

Cytotoxicity of test compounds is determined by MTT assay using Caco-2 cells in 96 well plates at a density of 20.000 cells per well in 200 µl FBS-free medium, grown for 96 h and followed by 24 h contact time with the test compounds (100 µL of serum-free media containing SE 0.5, 1, and 2 mg/mL; trans-crocin-1 250, 500, and 1000 µM; Transcrocetin 10, 40, 80, and 160 µM) and incubation at 37°C/5% CO2. The incubation solutions are aspirated, each well is washed twice with 150 µL of PBS and 50 µL of MTT solution are added (2.5 mg/mL in PBS). Supernatants are discarded and the formed formazan is dissolved in 50 µL of DMSO. The absorption of the resulting solution is determined at λ=492 nm against reference wavelength λ=690 nm[1].

Shanghai Jinpan Biotech Co Ltd has not independently confirmed the accuracy of these methods. They are for reference only.
参考文献

  • [1]. Lautenschläger M, et al. Intestinal formation of trans-Crocetin from saffron extract (Crocus sativus L.) and in vitro permeation through intestinal and blood brain barrier. Phytomedicine. 2015 Jan 15;22(1):36-44.

    [2]. José Bagur M, et al. Saffron: An Old Medicinal Plant and a Potential Novel Functional Food. Molecules. 2017 Dec 23;23(1). pii: E30.