SH30256.01B-GE 实验试剂 Hyclone PBS磷酸盐缓冲液(1x) 500ml

产品型号SH30256.01B

品       牌沃特曼

厂商性质代理商

产品简介

GE 实验试剂 Hyclone PBS磷酸盐缓冲液(1x) 500ml,PH值:7.0-7.2,0.0067M(PO4),不含钙、镁,0.1微米无菌过滤。

详情介绍

Hyclone磷酸盐缓冲液(1x) SH30256.01B

PH值:7.0-7.2
0.0067M(PO4)
不含钙、镁 0.1微米无菌过滤
液体培养基在使用的过程中要注意以下问题:
运输中要避光冷藏,瓶口保持向上,切勿剧烈振荡;
开瓶后不要长时间暴露在空气和强光下,以保持培养基的稳定性;
开瓶后能在一周内用完,短时间内用不完可分装冷藏保存,但PH值会在随后的时间里慢慢变碱,属于正常现象,请放心使用;
所有液体培养基均经100纳米无菌过滤和出厂严格检测,除非需要,请不要私自进行二次过滤和任何形式的无菌检测。

圣斯特Sciencetool实验室瓶顶式缓冲液真空抽滤装置FS300PMF

圣斯特Sciencetool实验室瓶顶式缓冲液真空抽滤装置FS300PMF

产品型号: FS300PMF 品  牌: 圣斯特Sciencetool

简介:
FS300PMF实验室缓冲液瓶顶式真空过滤装置是一款设计用于对各类缓冲液、流动相试剂进行除杂质澄清过滤的产品。其配置的过滤漏斗可以直接和普通的GL45螺纹口蓝盖玻璃试剂瓶相连,使过滤完后的液体可以直接进行保存,更方便减少了不必要的操作步骤。

特点:
1.整组过滤瓶采用瓶顶式结构,过滤漏斗可以直接和采用GL45规制的螺纹口蓝盖试剂瓶相连接,便于过滤完后直接拧上瓶盖保存,免去中间倒出滤液的麻烦,避免二次污染。
2.过滤漏斗采用PSF材质制作,具有良好的化学耐受性能,适宜于各类缓冲液、流动相溶剂的过滤。
3.标配包含自动进样盖:用户在过滤时,将进液管投入待过滤的液体中,开机抽真空形成内外压差后,待过滤液体自动吸入过滤杯并通过滤膜,免去了手工倒液的麻烦和操作时间。

4.抽滤泵采用无油式,插电即可使用,免维护,使用方便。进口端配有四合一套件,可以实现防滤液倒吸、阻挡粉尘颗粒物、观察真空度、调节压力和过滤速度的功能。
5.可以搭配的滤膜种类广泛,只需要直径为47mm,任意品牌、孔径和材质的过滤膜都可以配套使用。

应用领域:适宜于各类实验室溶剂、溶液的除菌、除杂质或澄清过滤,尤其是各类缓冲液、液相色谱流动相的过滤。

技术参数:
产品名称:实验室缓冲液瓶顶式真空过滤装置
品牌:圣斯特Sciencetool
型号:FS300PMF
●配置:含R300真空抽滤泵1台、300ml塑料瓶顶式过滤漏斗1支、漏斗杯盖1组、1000ml蓝盖试剂瓶1只、密封瓶盖1只、调节阀1组、缓冲杯1只、阻尘滤芯1只、带防倒吸保护器的真空泵管1组。
集液瓶种类:GL45螺纹口试剂瓶
漏斗材质:PSF
集液瓶材质:硼硅玻璃
真空度:0.089Mpa≈670mmHg
抽速:20 L/min
适合滤膜规格:直径-47mm
                        孔径-不限
                        材质-不限
调压调速方式:无级旋钮式
真空度显示方式:表针式真空计
防倒吸保护:疏水性阻水保护器
阻水保护器内芯材料:PTFE聚四氟乙烯
过滤漏斗容量:300ml
集液瓶容量:1000ml
泵管规格:耐压,无粉尘,总长1m
电压:220V/50Hz
噪音:50.0dB
功率:60W
外形尺寸:272×121×164mm
保修期:3年

更多过滤产品推介:

FS410V粘稠、高固体颗粒物含量快速真空抽滤装置:用于解决传统抽滤装置滤膜尺寸小易堵塞、抽滤泵真空度低吸力小的问题,可以大幅提升过滤效率。

VF204-SB1含1L缓冲瓶的无油过滤真空泵:外壳全不锈钢材质,由于表面光滑所以擦拭清洁滴溅在表面的溶剂或溶液。将缓冲瓶和过滤泵主机集合在一个机体中,起到防倒吸效果同时,还能缩减台面占用空间。

FU-CH全塑料溶剂过滤器:全塑料材质制作,既保持了塑料的耐摔、轻便易于使用的特性,同时由于采用了具有一定耐化学性能的PSF和PP材质,所以可以用于过滤PBS缓冲液、流动相溶剂、氢氟酸、磷酸等。

 

Bis-Tris

生化分析试剂 Biochemical Assay Reagents
Bis-Tris;

Bis-Tris 是一种常用的缓冲液。

Bis-Tris

Bis-Tris Chemical Structure

CAS No. : 6976-37-0

规格 价格 是否有货 数量
Free Sample (0.1-0.5 mg) ; Apply now ;
25 g ¥500 In-stock
50 g ; 询价 ;

* Please select Quantity before adding items.

Bis-Tris 相关产品

bull;相关化合物库:

  • Bioactive Compound Library Plus

生物活性

Bis-Tris is a commonly used buffer[1].

分子量

209.24

Formula

C8H19NO5

CAS 号

6976-37-0

运输条件

Room temperature in continental US; may vary elsewhere.

储存方式
Powder -20deg;C 3 years
4deg;C 2 years
In solvent -80deg;C 6 months
-20deg;C 1 month
溶解性数据
In Vitro:;

H2O : 500 mg/mL (2389.60 mM; Need ultrasonic)

配制储备液
浓度 溶剂体积 质量 1 mg 5 mg 10 mg
1 mM 4.7792 mL 23.8960 mL 47.7920 mL
5 mM 0.9558 mL 4.7792 mL 9.5584 mL
10 mM 0.4779 mL 2.3896 mL 4.7792 mL

*

请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液;一旦配成溶液,请分装保存,避免反复冻融造成的产品失效
储备液的保存方式和期限:-80°C, 6 months; -20°C, 1 month。-80°C 储存时,请在 6 个月内使用,-20°C 储存时,请在 1 个月内使用。

参考文献
  • [1]. Oh, Soon Jin, et al. Structure and phosphodiesterase activity of Bis-Tris coordinated lanthanide(III) complexes.

HEPPSO

生化分析试剂 Biochemical Assay Reagents
HEPPSO; 纯度: ge;98.0%

HEPPSO 是一种两性离子缓冲剂。HEPPSO 缓冲液的工作 pH 范围是 7.1-8.5。HEPPSO 显示出较高的结合铜 (II) 的能力,在 2.0 mM 缓冲液浓度下的 pKa 值为 7.84。

HEPPSO

HEPPSO Chemical Structure

CAS No. : 68399-78-0

规格 价格 是否有货 数量
Free Sample (0.1-0.5 mg) ; Apply now ;
10;mM;*;1 mL in DMSO ¥550 In-stock
500 mg ¥500 In-stock
1 g ; 询价 ;
5 g ; 询价 ;

* Please select Quantity before adding items.

HEPPSO 相关产品

bull;相关化合物库:

  • Bioactive Compound Library Plus

生物活性

HEPPSO is a zwitterionic buffer. The working pH range of HEPPSO buffer is 7.1-8.5. HEPPSO displays relatively high ability to bind copper(II), has a pKa of 7.84 at 2.0 mM buffer concentration[1][2].

分子量

268.33

Formula

C9H20N2O5S

CAS 号

68399-78-0

运输条件

Room temperature in continental US; may vary elsewhere.

储存方式
Powder -20deg;C 3 years
4deg;C 2 years
In solvent -80deg;C 6 months
-20deg;C 1 month
溶解性数据
In Vitro:;

H2O : 250 mg/mL (931.69 mM; Need ultrasonic)

DMSO : 250 mg/mL (931.69 mM; Need ultrasonic)

配制储备液
浓度 溶剂体积 质量 1 mg 5 mg 10 mg
1 mM 3.7268 mL 18.6338 mL 37.2675 mL
5 mM 0.7454 mL 3.7268 mL 7.4535 mL
10 mM 0.3727 mL 1.8634 mL 3.7268 mL

*

请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液;一旦配成溶液,请分装保存,避免反复冻融造成的产品失效
储备液的保存方式和期限:-80°C, 6 months; -20°C, 1 month。-80°C 储存时,请在 6 个月内使用,-20°C 储存时,请在 1 个月内使用。

In Vivo:

请根据您的实验动物和给药方式选择适当的溶解方案。以下溶解方案都请先按照 In Vitro 方式配制澄清的储备液,再依次添加助溶剂:

——为保证实验结果的可靠性,澄清的储备液可以根据储存条件,适当保存;体内实验的工作液,建议您现用现配,当天使用; 以下溶剂前显示的百
分比是指该溶剂在您配制终溶液中的体积占比;如在配制过程中出现沉淀、析出现象,可以通过加热和/或超声的方式助溶

  • 1.

    请依序添加每种溶剂:;PBS

    Solubility: 110 mg/mL (409.94 mM); Clear solution; Need ultrasonic

  • 2.

    请依序添加每种溶剂:;10% DMSO ;; 40% PEG300 ;; 5% Tween-80 ;; 45% saline

    Solubility: ≥ 4.17 mg/mL (15.54 mM); Clear solution

    此方案可获得 ≥ 4.17 mg/mL (15.54 mM,饱和度未知) 的澄清溶液。

    以 1 mL 工作液为例,取 100 μL 41.7 mg/mL 的澄清 DMSO 储备液加到 400 μL PEG300 中,混合均匀;向上述体系中加入50 μL Tween-80,混合均匀;然后继续加入 450 μL生理盐水定容至 1 mL。

    将 0.9 g 氯化钠,完全溶解于 100 mL ddH₂O 中,得到澄清透明的生理盐水溶液

  • 3.

    请依序添加每种溶剂:;10% DMSO ;; 90% (20% SBE-β-CD in saline)

    Solubility: ≥ 4.17 mg/mL (15.54 mM); Clear solution

    此方案可获得 ≥ 4.17 mg/mL (15.54 mM,饱和度未知) 的澄清溶液。

    以 1 mL 工作液为例,取 100 μL 41.7 mg/mL 的澄清 DMSO 储备液加到 900 μL 20% 的 SBE-β-CD 生理盐水水溶液中,混合均匀。

    将 2 g 磺丁基醚 β-环糊精加入 5 mL 生理盐水中,再用生理盐水定容至 10 mL,完全溶解,澄清透明
  • 4.

    请依序添加每种溶剂:;10% DMSO ;; 90% corn oil

    Solubility: ≥ 4.17 mg/mL (15.54 mM); Clear solution

    此方案可获得 ≥ 4.17 mg/mL (15.54 mM,饱和度未知) 的澄清溶液,此方案不适用于实验周期在半个月以上的实验。

    以 1 mL 工作液为例,取 100 μL 41.7 mg/mL 的澄清 DMSO 储备液加到 900 μL玉米油中,混合均匀。

  • 5.

    请依序添加每种溶剂:;10% DMSO ;; 40% PEG300 ;; 5% Tween-80 ;; 45% saline

    Solubility: ≥ 2.5 mg/mL (9.32 mM); Clear solution

    此方案可获得 ≥ 2.5 mg/mL (9.32 mM,饱和度未知) 的澄清溶液。

    以 1 mL 工作液为例,取 100 μL 25.0 mg/mL 的澄清 DMSO 储备液加到 400 μL PEG300 中,混合均匀;向上述体系中加入50 μL Tween-80,混合均匀;然后继续加入 450 μL生理盐水定容至 1 mL。

    将 0.9 g 氯化钠,完全溶解于 100 mL ddH₂O 中,得到澄清透明的生理盐水溶液

  • 6.

    请依序添加每种溶剂:;10% DMSO ;; 90% (20% SBE-β-CD in saline)

    Solubility: ≥ 2.5 mg/mL (9.32 mM); Clear solution

    此方案可获得 ≥ 2.5 mg/mL (9.32 mM,饱和度未知) 的澄清溶液。

    以 1 mL 工作液为例,取 100 μL 25.0 mg/mL 的澄清 DMSO 储备液加到 900 μL 20% 的 SBE-β-CD 生理盐水水溶液中,混合均匀。

    将 2 g 磺丁基醚 β-环糊精加入 5 mL 生理盐水中,再用生理盐水定容至 10 mL,完全溶解,澄清透明
  • 7.

    请依序添加每种溶剂:;10% DMSO ;; 90% corn oil

    Solubility: ≥ 2.5 mg/mL (9.32 mM); Clear solution

    此方案可获得 ≥ 2.5 mg/mL (9.32 mM,饱和度未知) 的澄清溶液,此方案不适用于实验周期在半个月以上的实验。

    以 1 mL 工作液为例,取 100 μL 25.0 mg/mL 的澄清 DMSO 储备液加到 900 μL玉米油中,混合均匀。

*以上所有助溶剂都可在 MCE 网站选购。
参考文献
  • [1]. Vasconcelos MT, et, al. Copper(II) complexation properties and surfactant activity of 3-[N, N-bis(2-hydroxyethyl)amino]-2-hydroxypropanesulfonic acid and N-(2-Hydroxyethyl)piperazine-N’-2-hydroxypropanesulfonic acid pH buffers which may affect trace metal speciation in in vitro studies. Anal Biochem. 1998 Dec 15; 265(2): 193-201.

    [2]. Mash HE, et, al. Complexation of copper by zwitterionic aminosulfonic (good) buffers. Anal Chem. 2003 Feb 1; 75(3): 671-7.

土壤DNA萃取的选择缓冲液 Lysis Solution BB SP1

土壤DNA萃取的选择缓冲液
Lysis Solution BB SP1

  • 产品特性
  • 相关资料
  • Q&A
  • 参考文献

土壤DNA萃取的选择缓冲液土壤DNA萃取的选择缓冲液                              Lysis Solution BB SP1

Lysis Solution BB SP1


◆原理


  LYSIS Solution BB SP1 是是 ISOIL For Beads Beating 专用的选择性缓冲液。使用 ISOIL For Beads Beating,在如黑土这些含水铝石英很多的土壤中萃取 DNA 时 ISOIL For Beads Beating 能够代替试剂盒附属的 LYSIS Solution BB 使用。使用本产品能够增加含水铝石英很多的黑土的 DNA 萃取量。

产品列表
产品编号 产品名称 产品规格 产品等级 备注
313-06221 Lysis Solution BB SP1 
土壤DNA 萃取的选择缓冲液
50mL

GE 实验试剂 Hyclone PBS磷酸盐缓冲液(1x) 500mlSH30256.01B

GE 实验试剂 Hyclone PBS磷酸盐缓冲液(1x) 500ml

简要描述:

PH值:7.0-7.2
0.0067M(PO4)
不含钙、镁 0.1微米无菌过滤
液体滤膜在使用的过程中要注意以下问题:
运输中要避光冷藏,瓶口保持向上,切勿剧烈振荡;
开瓶后不要长时间暴露在空气和强光下,以保持滤膜的稳定性;
开瓶后能在一周内用完,短时间内用不完可分装冷藏保存,但PH值会在随后的时间里慢慢变碱,属于正常现象,请放心使用;
所有液体滤膜均经100纳米无菌过滤和出厂严格检测,除非需要,请不要私自进行二次过滤和任何形式的无菌检测。

新型 PCR 扩增缓冲液 Ampdirect®Gene Amplification

新型 PCR 扩增缓冲液
Ampdirect®Gene Amplification

  • 产品特性
  • 相关资料
  • Q&A
  • 参考文献

Ampdirect® Gene Amplification新型 PCR 扩增缓冲液                              Ampdirect®Gene Amplification

新型 PCR 扩增缓冲液

  ——无需抽提 DNA,直接 PCR 扩增!



新型 PCR 扩增缓冲液                              Ampdirect®Gene Amplification

  Ampdirect® Gene Amplification 是一款新开发的 PCR 缓冲液,可有效降低 PCR 反应体系里的蛋白质、糖类等各种 PCR 抑制物对 PCR 扩增的影响。应用广泛,适合对昆虫、植物、微生物、土壤、血液、石蜡切片等各类样品直接进行 PCR 扩增。



◆优势

● 省时省力省钱

   无需 DNA 抽提,直接进行 PCR 反应,节省时间

● 适用于微量样品

   因为不需要 DNA 抽提,不会产生样品损失,故此十分适用于微量样品

● 热启动酶

   厂家自行开发的高性能聚合酶 BIOTAQ ™

● 支持后续实验操作

   扩增产物可用于后续的测序和 RFLP 等片段分析



◆实验流程


新型 PCR 扩增缓冲液                              Ampdirect®Gene Amplification



◆原理

新型 PCR 扩增缓冲液                              Ampdirect®Gene Amplification



◆示例

新型 PCR 扩增缓冲液                              Ampdirect®Gene Amplification


新型 PCR 扩增缓冲液                              Ampdirect®Gene Amplification

注:动物样本,比如血液和黏膜细胞等可以直接加入 PCR 反应液,不需消化处理。固体样本,比如植物和动物组织需要在含有 SDS 和蛋白酶 K 的

       消化液中处理后再进行 PCR 反应,或者使用 FTA 卡收集植物组织中的 DNA,取带有 DNA 的 FTA 卡加入 PCR 反应液进行PCR 反应。



◆其他应用


● 血清中病毒检出

● 小鼠尾巴裂解液检测基因分型

● 植物、血液/ 滤纸血液、土壤

● 昆虫、石蜡切片等微量样品

   其他应用的详细内容请看相关资料!


相关资料


Ampdirect 推荐使用酶与不推荐使用酶


新型 PCR 扩增缓冲液                              Ampdirect®Gene Amplification

◆其他应用


新型 PCR 扩增缓冲液                              Ampdirect®Gene Amplification

Ampdiret 相关的FAQ


◆有关样品以及样品前处理

1.采集后长期保存的血液作为模板,能进行 PCR 吗?

实验证明,采集后冻存了长达五年时间的血或纸血(干燥血)作为模板,依然能进行PCR。必须长期稳定保存血液时,可使用 Whatman 公司的 FTA Card(核酸保存用卡)进行保存。关于血液(全血)对 PCR 的抑制作用,直接采集的血液(新鲜血)对 PCR 抑制作用更强。

另外,长久以来的经验证明,采用干燥血作为 PCR 模板时,对比用一般的实验纸保存,使用 FTACard 保存的血液进行 PCR 的结果更稳定,因此推荐使用 FTA Card 保存的血作为 PCR 模板。


2从粪便中抽提的 DNA 模板,可用 PCR 检测出细菌吗?

粪便抽提的 DNA 对 PCR 有抑制作用,因此用稀释后的 DNA 反应。从粪便样品中简便进行 PCR 的应用例子,在“使用实时 PCR 法高灵敏度检测 Vero Toxin”中有介绍。将10%的粪便悬浊液热处理(95℃,5 分钟)后,将离心后的上清液作为 PCR 模板。实验证明,使用这个方法,大部分的粪便样品都能稳定进行 PCR 。


3Ampdirect 可应用于 RT-PCR 吗?粪便里存在的 RNA 病毒也可以检测出来吗?

从粪便样品中简便检测 RNA 病毒的应用例子,可见“粪便中的 RNA 病毒检测”。将粪便悬浊液离心后的上清液,涂布在 Whatman 公司的 FTA Card(核酸保存用卡)上,干燥后,在卡上打(φ1.25 mm)就可作为模板使用。

Ampdirect Plus 可做为逆转录反应的反应液,逆转录酶选用的是 Invitrogen 公司的 M-MLVReverse Transcriptase(Cat No. 28025-013)。


4样品的溶解处理最后步骤要在95℃,进行5分钟的热处理,这个热处理步骤是必须的吗?

因为要使溶解液中的 Proteinase K 失活,所以必须热处理。如果不进行热处理,Proteinase K 仍保持活性的话,在往 PCR 反应液里加入溶解液时,Proteinase K 会将 PCR 酶(Taq DNA Polymerase)降解掉,从而不能进行稳定的 PCR。实际上,这是从大部分得不到目的 PCR 产物的例子上取得的经验。所以,必须在最后对溶解液进行热处理(95℃,5 分钟)。


5从植物的叶片抽提的 DNA 作为 PCR 模板进行反应,但得不到目的 PCR 产物。考虑到是植物里所含的糖类抑制了 PCR,使用 Ampdirect 可以改善这个情况吗?

至今为止,已有非常多客户反映,从实验植物、谷物、蔬菜、果蔬的叶片取样,用 Ampdirect 进行 PCR,都取得了良好的结果。不同植物对 PCR 的抑制作用也不尽相同,可参照“从植物取样的简便 PCR 实例”。在 PCR 前用溶解液进行前处理。如担心因为 PCR 被抑制而得不到目的 PCR 产物,用溶解处理液稀释5~10 倍,会对此情况有很大改善。


6小鼠尾部用溶解液溶解了大约1个小时,还没完全溶解。可作为 PCR 的模板使用吗?

将小鼠尾巴1~5mm 浸入 100 μL 溶解液内,55℃下溶解约1小时后,如小鼠尾巴还未完全溶解,对 Proteinase K 进行失活处理(95℃,5 分钟)后,从溶解液中提取出来的 DNA 量(拷贝数)应该足以作为 PCR 模板,大部分情况下进行 PCR 没有问题。

与此对照,如果是将大条的小鼠尾巴(5 mm 以上)过夜处理以至完全溶解,溶解液会变成高粘度状态。这种情况下,过剩的 DNA 反而会抑制 PCR 反应,请用蒸馏水或 TE 将溶解液稀释 10 倍左右。请注意尽量不要让小鼠尾巴过度溶解。

另外,溶解处理后的溶解液不需离心,只需取上清液就可作为 PCR 模板使用。从以往经验得知,经溶解处理过的溶解液在冷藏保存条件下保存2~3年内皆可作为 PCR 模板使用。


7不对唾液进行前处理就直接作为 PCR 模板是不可行的吧?“取样自口腔粘膜的 PCR”这个应用,是对口腔黏膜细胞用溶解液进行溶解处理,如果是用唾液的话,也必须用溶解液进行溶解处理吗?

目前,也有不少客户为了检测唾液中的微生物,不进行前处理,直接将唾液当成 PCR 模板使用。当作为模板进入 PCR 反应液的唾液中(数 μL)有大量微生物,可获得良好的 PCR 产物。与之相反,若微生物数量较少,根据唾液中的蛋白等物质对 PCR 抑制的不同程度,有可能得不到稳定的 PCR 产物。因此,推荐对溶液用溶解液进行溶解处理,再作为 PCR 模板。对唾液进行了溶解处理的话,可得到以下两种结果,①“抽提微生物的DNA”和②“降低唾液对 PCR 的抑制作用(分解唾液中的蛋白)”,也可得到稳定的 PCR 结果。


8如何用 PCR 检测血浆、血清样品中的微生物?

血浆、血清这些全血,对PCR 有较强抑制作用,所以不能将全血直接用作 PCR 模板。因此,要用于“检测血清中的病毒”时,将血浆、血清用溶解液进行溶解处理,分解掉血浆、血清中的蛋白,降低对PCR 的抑制作用。

请注意,本方法适用于检测 DNA 病毒、细菌,不可用于检测 RNA 病毒。检测 RNA 病毒时,在溶解处理步骤,因为血浆、血清中存在 RNA 分解酶,从病毒外壳裸露出来的 RNA 就会被分解。


9、使用 FTA Card 和实验纸作为模板进行多样品 PCR 时,因为使用打孔器等工具可能会引起污染。有没有相应的对策呢?

采集血液样品时,首次打孔取样后,在下一次取样前,用打孔器在 FTA Card 或实验纸上没有血样的空白处空打3个孔,这样可保证打孔器上不会残留前一样品的模板(血样是白血球 DNA)。因为从客户处得知,“每次用打孔器取样时,用金伯利(Kimwipe)浸渍酒精擦拭打孔器的打孔部分,这个方法反而引起污染”,所以推荐空打孔的方法。但是,检测拷贝数多的 DNA,如线粒体 DNA 和质粒 DNA 等时,本方法不能防止污染。

参考文献:


Zoophilic feeding behaviour of phlebotomine sand flies in the endemic areas of cutaneous leishmaniasis of Sindh Province, Pakistan. Tiwananthagorn S, et al.

The sequestrate genus Rosbeeva T.Lebel & Orihara gen. nov. (Boletaceae) from Australasia and Japan: new species and new combinations. Lebel T, et al.

Tandem repeat inserts in 13S globulin subunits, the major allergenic storage protein of common buckwheat (Fagopyrum esculentum Moench) seeds. Khan N, et al.

Parasitol Res. 2012 Jan 14

・     Zoophilic feeding behaviour of phlebotomine sand flies in the endemic areas of cutaneous leishmaniasis of Sindh Province, Pakistan.

     Tiwananthagorn S, et al.

样品:sand fly(沙蝇)、Leishmania(原虫)


Fungal Diversity (2012) 52: 49-71

・     The sequestrate genus Rosbeeva T.Lebel & Orihara gen. nov. (Boletaceae) from Australasia and Japan: new species and new combinations.

     Lebel T, et al.

样品:菌類、FTA card        


Food Chemistry Volume 133, Issue 1, 1 July 2012, Pages 29-37

・     Tandem repeat inserts in 13S globulin subunits, the major allergenic storage protein of common buckwheat (Fagopyrum esculentum Moench) seeds.

     Khan N, et al.

样品:荞麦(種子)

    

Acta Trop. 2012 Feb; 121(2): 93-8

・     Genotyping of sand fly species in Peruvian Andes where leishmaniasis is endemic.

     Fujita M, et al.

样品:sand fly(沙蝇)

      

Zool J Linn Soc. 2012 Feb; 164(2): 304-27

・     Revision of the Euthalia phemius complex (Lepidoptera: Nymphalidae) based on morphology and molecular analyses.

     Yago M, et al.

样品:昆虫(鱗翅目)

 

Limnology (3 November 2011), pp. 1-5

・     Surveillance of fish species composition using environmental DNA.

     Minamoto T, et al.

样品:Environmental water

    

Am. J. Pot Res (2011) 88: 500-10

・     Characterization of Crossability in the Crosses between Solanum demissum and S. tuberosum, and the F1 and BC1 Progenies.

     Sanetomo R, et al.

样品:土豆(種子) 

    

Systematic Botany (2011) 36(4): 836-44

・     A New Allotetraploid Species of Osmunda (Osmundaceae).

     Tsutsumi C, et al.

样品:蕨类植物(根、叶) 

Am. J. Pot Res

・     Germplasm Release: Saikai 35, a Male and Female Fertile Breeding Line Carrying Solanum Phureja-Derived Cytoplasm and Potato Cyst Nematode Resistance (H1) and Potato Virus Y Resistance (Rychc) Genes.

     Mori K, et al.

样品:土豆     


Neurochem Res. 2011 Nov;36(11):2127-35.

・     Ceruloplasmin protects against rotenone-induced oxidative stress and neurotoxicity.

     Hineno A, et al.

样品:小鼠   

 

Bone. 2011 Nov;49(5):1027-36.

・    Insertional mutation in the Golgb1 gene is associated with osteochondrodysplasia and systemic edema in the OCD rat.

     Katayama K, et al.

 

样品:大鼠  

  

Mycoscience

・    The genus Ponticulomyces (Physalacriaceae, Agaricales) from Japan.

     Ushijima S, et al.

样品:菌類、FTA card     

 

Trans R Soc Trop Med Hyg. 2011 Oct;105(10):561-7.

・    Leishmania species identification using FTA card sampling directly from patients’ cutaneous lesions in the state of Lara, Venezuela.

      Kato H, et al.

样品:原虫(Leishmania)、FTA card  

 

Anticancer Res. 2011 Oct;31(10):3607-13.

・    Identification of The Distinctive Type i/XhoI+ Strain of Epstein-Barr Virus in Gastric Carcinoma in Peru.

     Ordonez P, et al.

样品:EB病毒    

分析手法:PCR-RFLP法          

 

Mol Genet Metab. 2011 Sep 10.

・    Newborn screening for Pompe disease in Japan.

     Oda E, et al.

样品:人体血液/滤纸血 

 

Blood. 2011 Sep 29.

・    Developmental origins and impact of BCR-ABL1 fusion and IKZF1 deletions in monozygotic twins with Ph+ acute lymphoblastic leukaemia.

     Cazzaniga G, et al.

样品:滤纸血   

      

Mol Biol Evol. 2011 Sep 22.

・    Entangling Ancient Allotetraploidization in Asian Mitella: An Integrated Approach for Multilocus Combinations.

     Okuyama Y, et al.

样品:植物 

  

Parasitol Res. 2011 Jul 8.

・    Prevalence of Trypanosoma sp. in cattle from Tanzania estimated by conventional PCR and loop-mediated isothermal of amplification (LAMP).

     Laohasinnarong D, et al.

样品:牛血液、原虫(锥虫) 

 

J Agric Food Chem. 2011 Jul 13;59(13):6856-63.

・    Practicable group testing method to evaluate weight/weight GMO content in maize grains.

     Mano J, et al.

样品:植物(玉米)

 

J Parasitol. 2011 Jun 14.

・    Molecular prevalence of different genotypes of theileria orientalis detected from cattle and water buffaloes in thailand.

     Altangerel K, et al.

样品:牛、水牛血液、原虫 

  

Euphytica

・    Reciprocal differences in DNA sequence and methylation status of the pollen DNA between F1 hybrids of Solanum tuberosum × S. demissum.

     Sanetomo R, et al.

样品:植物花粉(土豆) 

 

J Vet Sci. 2011 Jun;12(2):191-3.

・    A simplified PCR assay for fast and easy mycoplasma mastitis screening in dairy cattle.

     Higuchi H, et al.

样品:牛乳、細菌  

 

Biol Pharm Bull. 2011;34(5):779-82.

・    Identification of dendrobium species used for herbal medicines based on ribosomal DNA internal transcribed spacer sequence.

     Takamiya T, et al.

样品:植物  

 

Zootaxa 2905: 33-56 (3 Jun. 2011)

・    A survey of morphological variation in adult Meristogenys amoropalamus (Amphibia, Anura, Ranidae), with a description of a new cryptic species.

     Shimada T, et al.

样品:青蛙

    

Plant Syst Evol (2011) 292: 177-188.

・    Phytogeographic aspects of Lysionotus pauciflorus sensu lato (Gesneriaceae) in the China, Japan and Taiwan regions: phylogenetic and morphological relationships and taxonomic consequences.

     Kokubugata G, et al.

样品:植物  

 

FEMS Microbiol Ecol. 2011 Jan 11.

・    Microbial diversity with dominance of 16S rRNA gene sequences with high GC contents at 74℃ and 98℃ subsurface crude oil deposits in Japan.

     Yamane K, et al.

样品:石油中采集的细菌   

 

J Mol Neurosci. 2011 Feb;43(2):217-24.

・    Decreased intake of sucrose solutions in orexin knockout mice.

     Matsuo E, et al.

样品:小鼠   

             

Glycoconj J. 2010 Dec 21.

・    Excretion into feces of asialo GM1 in the murine digestive tract and Lactobacillus johnsonii exhibiting binding ability toward asialo GM1. A possible role of epithelial glycolipids in the discharge of intestinal bacteria.

     Iwamori M, et al.

样品:細菌(Lactobacillus johnsonii、Lactobacillus casei)   

 

Mycologia. 2010 Dec 21.

・    Two species of Strobilomyces (Boletaceae, Boletales), S. seminudus and S. hongoi sp. nov. from Japan.

     Sato H, et al.

样品:菌類  

 

Molecular Ecology Resources. 2010 Oct 28.

・    A primer set to determine sex in the small Indian mongoose, Herpestes auropunctatus.

     Murata C, et al.

 样品:動物(猫鼬)

 

Entomological Science 2010 13: 303-310

・    An alien Sennertia mite (Acari: Chaetodactylidae) associated with an introduced Oriental bamboo-nesting large carpenter bee (Hymenoptera: Apidae: Xylocopa) invading the central Honshu Island, Japan.

     Kawazoe K, et al.

样品:木匠蜂螨

  

Vector Borne Zoonotic Dis. 2010 Oct 18.

・    Natural Infections of Man-Biting Sand Flies by Leishmania and Trypanosoma Species in the Northern Peruvian Andes.

     Kato H, et al.

样品:原虫(Leishmania and Trypanosoma Species) 

 

BMC Ecol. 2010 Oct 15;10:21.

・    Genetic structure of the oak wilt vector beetle Platypus quercivorus: inferences toward the process of damaged area expansion.

     Shoda-Kagaya E, et al.

样品:昆虫  

 

Mycoscience. 2010 Oct 30.

・    Rapid detection for sporeless trait from Pleurotus pulmonarius culture extracts by using real-time PCR.

     Okuda Y, et al.

样品:菌類  

分析手法:融解温度解析          

 

IBC 2010, vol.2, article no.12, pp.1-7

・    Genetic Quality Control of the Rat Strains at the National Bio Resource Project – Rat.

     Kuramoto T, et al.

样品:大鼠、FTA card     

分析手法:Amp-FTA method              

 

Int J Environ Res Public Health. 2010 Mar;7(3):814-26.

・    Molecular epidemiology for vector research on leishmaniasis.

     Kato H, et al.

样品:昆虫、原虫    

分析手法:PCR-RFLP法            

   

Am J Sports Med. 2010 Aug 19.

・    Cartilage Intermediate Layer Protein Gene Is Associated With Lumbar Disc Degeneration in Male, but Not Female, Collegiate Athletes.

     Min SK, et al.

样品:人口腔粘膜細胞              

   

BMC Evol Biol. 2010 Jun 18;10:185.

・    Geographic variation in the damselfish-red alga cultivation mutualism in the Indo-West Pacific.

     Hata H, et al.

样品:植物(紅藻)           

 

 

      

J Biochem. 2010 Aug 10.

・    NF-kappaB regulates the expression of Nucling, a novel apoptosis regulator, with involvement of proteasome and caspase for its degradation.

     Tran NH, et al.

样品:小鼠           

 

Med Mycol. 2010 Jun;48(4):665-8.

・    Nourseothricin acetyltransferase: a new dominant selectable marker for the dermatophyte Trichophyton mentagrophytes.

     Alshahni MM, et al.

样品:真菌           

 

J Plant Res. 2010 May 15.

・    Molecular database for classifying Shorea species (Dipterocarpaceae) and techniques for checking the legitimacy of timber and wood products.

     Tsumura Y, et al.

样品:植物           

 

Forensic Toxicology 2010 July Volume 28, Number 2, 1-7

・    Chemical constituents and DNA sequence analysis of a psychotropic herbal product.

     Kikuchi H, et al.

样品:植物           

 

J Clin Microbiol. 2010 Aug 18.

・    Use of FTA Cards for Direct Sampling of Patients' Lesions in the Ecological Study of Cutaneous Leishmaniasis.

     Kato H, et al.

样品:原虫、FTA card         

   

Vet Parasitol. 2010 Aug 4;171(3-4):207-15.

・    Generation of IFN-gamma-producing cells that recognize the major piroplasm surface protein in Theileria orientalis-infected bovines.

     Yamaguchi T, et al.

样品:牛血液、原虫             

 

Jpn J Infect Dis. 2010 May;63(3):173-80.

・    Development of triplex SYBR green real-time PCR for detecting Mycoplasma pneumoniae, Chlamydophila pneumoniae, and Legionella spp. without extraction of DNA.

     Kerdsin A, et al.

样品:    細菌 (Mycoplasma pneumoniae、Chlamydophila pneumoniae、Legionella spp.)

分析手法:    real-time PCR    

 

Am J Physiol Renal Physiol. 2010 Jun;298(6):F1341-50.

・    Phosphaturic action of fibroblast growth factor 23 in Npt2 null mice.

     Tomoe Y, et al.

样品:小鼠           

 

Exp Eye Res. 2010 Jul;91(1):26-33.

・    A novel middle-wavelength opsin (M-opsin) null-mutation in the retinal cone dysfunction rat.

     Xie B, et al.

样品:小鼠、FTA card         

  

Am. J. Botany 2010 97: 373-387.

・    Developmental morphology of seedling and shoot and phylogenetic relationship of Diplobryum koyamae (Podostemaceae).

     Koi S, et al.

样品:植物           

 

Food Control Volume 21, Issue 5, May 2010, Pages 599-605

・    Meat species identification based on the loop mediated isothermal amplification and electrochemical DNA sensor.

     Ahmed MU, et al.

样品:食肉           

 

J Virol Methods. 2010 Feb;163(2):282-6.

・    Detection of noroviruses in fecal specimens by direct RT-PCR without RNA purification.

     Nishimura N, et al.

样品:RNA病毒          

   

Theor Appl Genet. 2010 Jan;120(2):205-14.

・    DNA methylation in diploid inbred lines of potatoes and its possible role in the regulation of heterosis.

     Nakamura S, et al.

 

样品:植物(potato)     

分析手法:RAPD法          

 

Genes Genet Syst. 2009 Oct;84(5):371-8.

・    Comparative differentiation in mitochondrial and chloroplast DNA among cultivated potatoes and closely related wild species.

     Hosaka K, et al.

样品:植物(土豆)     

分析手法:PCR-RFLP法          

   

J Fish Dis. 2009 Oct;32(10):857-64.

・    Distribution of the introduced cyprinid herpesvirus 3 in a wild population of common carp, Cyprinus carpio L.

     Uchii K, et al.

样品:魚類(鲤鱼)、DNA病毒        

     

Plant Physiol. 2009 Dec;151(4):2046-57.

・    The phytochrome-interacting factor PIF7 negatively regulates DREB1 expression under circadian control in Arabidopsis.

     Kidokoro S, et al.

样品:植物(拟南芥)              

  

Divers. Distrib. 15 (6), 917-927 (2009)

・    Chloroplast DNA phylogeography of the endangered Japanese red maple (Acer pycnanthum): the spatial configuration of wetlands shapes genetic diversity.

     Saeki I, et al.

样品:植物(枫树)           

 

Indian Journal of Science and Technology Vol.2 No. 10 (Oct 2009)

・    Development of activated sludge adapted to high concentrations of phenol and enhancement of its phenol removal ability by addition of a processed lignite.

     Ohtsuki T, et al.

样品:活性汚泥        

分析手法:PCR-DGGE法         

 

Mol Ecol. 2009 Dec;18(23):4904-11.

・    Hybridization involving independent gametophytes in the Vandenboschia radicans complex (Hymenophyllaceae): a new perspective on the distribution of fern hybrids.

     Ebihara A, et al.

样品:植物(蕨类)     

分析手法:PCR-SSCP法          

  

Appl Physiol Nutr Metab. 2009 Oct;34(5):926-32.

・    Is there a gender difference between ACE gene and race distance?

     Min SK, et al.

样品:人 口腔粘膜細胞            

 

Int J Sports Med. 2009 Sep;30(9):691-4.

・    The cartilage intermediate layer protein gene is associated with lumbar disc degeneration in collegiate judokas.

     Min SK, et al.

样品:人口腔粘膜細胞              

 

Biosci Biotechnol Biochem. 2009 Nov;73(11):2452-9.

・    Genome-wide identification, structure and expression studies, and mutant collection of 22 early nodulin-like protein genes in Arabidopsis.

     Mashiguchi K, et al.

样品:植物(拟南芥)              

 

Plant Biotechnology 26(4), 435-441, 2009

・    Modification of the surface carbohydrate composition of tobacco protoplasts transformed with the human UDP-galactose transporter gene hUGT1

     Horibe T, et al.

样品:植物(烟草)           

  

Genes Brain Behav. 2009 Oct;8(7):650-60.

・    A spontaneous mutation of the Wwox gene and audiogenic seizures in rats with lethal dwarfism and epilepsy.

     Suzuki H, et al.

样品:大鼠           

 

J Plant Res 2009; 122: 585-95

・    Genetic population structure of Osmunda japonica, rheophilous Osmunda lancea and their hybrids

     Yatabe Y, et al.

样品:植物           

 

Am J Physiol Renal Physiol 2009; 297: F671-8

・    Npt2a and Npt2c in mice play distinct and synergistic roles in inorganic phosphate metabolism and skeletal development

     Segawa H, et al.

样品:小鼠           

 

J Immunol 2009;183: 3053-63

・    Crucial contribution of thymic Sirp alpha+ conventional dendritic cells to central tolerance against blood-borne antigens in a CCR2-dependent manner

     Baba T, et al.

样品:小鼠           

  

J Nat Med 2009; 63: 340-4

・    The botanical origin of kratom (Mitragyna speciosa; Rubiaceae) available as abused drugs in the Japanese markets

     Maruyama T, et al.

样品:植物         

分析手法:PCR-RFLP法          

 

Emerg Infect Dis 2009;15: 912-5

・    Bartonella quintana in body lice and head lice from homeless persons, San Francisco, California, USA

     Bonilla DL, et al.

样品:虱子、細菌              

  

Plant Cell Physiol 2009; 50: 1579-86

・    Arabidopsis bile acid:sodium symporter family protein 5 is involved in methionine-derived glucosinolate biosynthesis

     Sawada Y, et al.

样品:植物(拟南芥)              

 

Mol Genet Metab 2009; 97: 190-5

・    High frequency of acid alpha-glucosidase pseudodeficiency complicates newborn screening for glycogen storage disease type II in the Japanese population

     Kumamoto S, et al.

样品:滤纸血        

分析手法:ARMS-PCR法         

 

Laboratory Animal research 2009; 25: 75-8

・    Simple Genotyping Method Using Ampdirect Plus and FTA Technologies: Application to the Identification of Transgenic Animals and Their Routine Genetic Monitoring

     Nakanishi S, et al.

样品:小鼠-大鼠、FTA card               

 

Int. J. Environ. Res. Public Health 2009; 6: 999-1009

・    Association between a Polymorphism of Aminolevulinate Dehydrogenase (ALAD) Gene and Blood Lead Levels in Japanese Subjects

     Miyaki K, et al.

样品:人全血              

 

Jpn J Infect Dis 2009; 62: 164-7

・    Direct Colony PCR of Several Medically Important Fungi using Ampdirect(R) Plus

     Alshahni MM, et al.

样品:真菌(酵母・霉菌)              

 

Biochem. Eng J 2009; 45: 76-81

・    Characterization of bacterial population of raw milk from bovine mastitis by culture-independent

PCR-DGGE method

     Kuang Y, et al.

样品:牛乳、細菌     

分析手法:PCR-DGGE法           

 

Lab Chip 2009; 9: 1052-8

・    Microfluidic device using chemiluminescence and a DNA-arrayed thin film transistor photosensor for single nucleotide polymorphism genotyping of PCR amplicons from whole blood

     Hatakeyama K, et al.

样品:人全血      

分析手法:PCR-RFLP法          

 

Cancer Cell 2009; 15: 195-206

・    Cancer metastasis is accelerated through immunosuppression during Snail-induced EMT of cancer cells

     Kudo-Saito C, et al.

 

Vet Microbiol 2009; 135: 261-6

・    Detection of cyprinid herpesvirus 3 DNA in river water during and after an outbreak

     Minamoto T, et al.

样品:河川水、DNA病毒         

 

Methods Mol Biol 2009; 538: 7-27

・    Backtracking of leukemic clones to birth

     Wiemels J, et al.

样品:新生儿滤纸血           

 

Int J Plant Sci 2009; 170: 237-46

・    Phylogenetic relationship and morphology of Dalzellia gracilis (Podostemaceae, subfamily Tristichoideae)

with proposal of a new genus

     Koi S, et al.

样品:植物           

 

Infect Dis Clin Pract 2008; 16: 230-4

・    Association of the SLC11A1 Gene Polymorphisms With Susceptibility to Micobacterium Infections in a Japanese Population

     Asai S, et al.

 

J Vet Diagn Invest 2008; 20: 68-71

・    Molecular screening of canine GM1 gangliosidosis using blood smear specimens after prolonged storage:

detection of carriers among shiba dogs in northern Japan

     Yamato O, et al.

 

Biosci Biotechnol Biochem 2008; 72: 2831-9

・    Diversity and Similarity of Microbial Communities in Petroleum Crude Oils Produced in Asia

     Yamane K, et al.

 

Vector Borne Zoonotic Dis 2008; 8: 565-73

・    Detection of Trypanosoma brucei in field-captured tsetse flies and identification of host species fed on by the infected flies

     Konnai S, et al.

 

J. Pestic Sci 2008; 33: 122-7

・    Environmental distribution and novel high-throughput screening of APEO-degrading bacteria using matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-MS)

     Ichiki Y, et al.

 

Zootaxa 2008; 1746: 15-38

・    Molecular systematics and biogeography of the genus Zizina(Lepidoptera: Lycaenidae)

     Yago M, et al.

 

Extremophiles 2008; 12: 519-27

・    Phylogenetic and enzymatic diversity of deep subseafloor aerobic microorganisms in organics- and methane-rich sediments off Shimokita Peninsula

     Kobayashi T, et al.

 

Principles and Technical Aspects of PCR Amplification

・    pp91-101, Springer Netherlands, 2008

     Elizabeth van Pelt-Verkuil, et al.

 

Zoolog Sci 2008; 25: 838-42

・  Phylogenetic Position of the Endemic Large Carpenter Bee of the Ogasawara Islands, Xylocopa ogasawarensis (Matsumura, 1912) (Hymenoptera: Apidae), Inferred from Four Genes

     Kawazoe K, et al.

 

Mol Phylogenet Evol 2008; 49: 503-13

・    Redundant species, cryptic host-associated divergence, and secondary shift in Sennertia mites (Acari: Chaetodactylidae) associated with four large carpenter bees (Hymenoptera: Apidae: Xylocopa) in the Japanese island arc

     Kawazoe K, et al.

 

Odonatologica 2008; 37: 131-44

・    Population genetic differentiaiton in three sympatric damselfly species in a highly fragmented urban landscape

     Sato M, et al.

 

Blood 2008; 111: 376-8

・    NOTCH1 mutation can be an early, prenatal genetic event in T-ALL

     Eguchi-Ishimae M, et al.

      

Res Vet Sci 2007; 82: 54-60

・    Nonsense mutation of feline beta-hexosaminidase beta-subunit (HEXB) gene causing Sandhoff disease in a family of Japanese domestic cats

     Kanae Y, et al.

 

Leuk Res 2007; 31: 1633-40

・    Regulatory polymorphisms of multidrug resistance 1 (MDR1) gene are associated with the development of childhood acute lymphoblastic leukemia

     Hattori H, et al.

 

Microbiol Immunol 2007; 51: 507-17

・    Plasma levels of unactivated thrombin activatable fibrinolysis inhibitor (TAFI) are down-regulated in young

adult women: analysis of a normal Japanese population

     Akatsu H, et al.

    

Am J Botany 2007; 94: 1630-41

・    Cryptic species and host specificity in the ectomycorrhizal genus Strobilomyces (Strobilomycetaceae)

     Sato H, et al.

 

Botanical Journal of the Linnean Society 2007; 155, 1-27

・    A global molecular phylogeny of the fern genus Trichomanes (Hymenophyllaceae) with special reference to stem anatomy

     Ebihara A, et al.

 

Trop Anim Health Prod 2007; 39: 369-74

・    Comparison of polymerase chain reaction methods for the detection of Theileria equi infection using whole blood compared with pre-extracted DNA samples as PCR templates

     Alhassan A, et al.

  

Am J Trop Med Hyg 2007; 77: 324-9

・    Establishment of a mass screening method of sand fly vectors for Leishmania infection by molecular biological methods.

     Kato H, et al

 

Metabolism 2006; 55: 751-7

・    G-protein beta 3 subunit polymorphism C1429T and low-density lipoprotein receptor-related protein 5 polymorphism A1330V are risk factors for hypercholesterolemia in Japanese males–a prospective study over 5 years

     Suwazono Y, et al.

   

J Vet Med Sci 2006; 68: 27-33.

・    Sequence variation of bovine prion protein gene in Japanese cattle (Holstein and Japanese Black)

     Nakamitsu S, et al.

  

Ann Hum Genet 2006; 70: 767-77

・    G-protein beta3 subunit variant C825T is a risk factor for hypertension in Japanese females -a prospective cohort study over 5 years

     Suwazono Y, et al.

  

J Biosci Bioeng 2006; 102: 572-4

・    High-throughput genotyping of filamentous fungus Aspergillus oryzae based on colony direct polymerase chain reaction.

     Suzuki S, et al

 

Biosci Biotechnol Biochem 2006; 70: 2387-93

・    The molecular phylogeny of the genus Rhizopus based on rDNA sequences.

     Abe A, et al

 

Appl Environ Microbiol 2006; 72: 7912-5

・    Molecular detection of epiphytic Acaryochloris spp. on marine macroalgae.

     Ohkubo S, et al

 

Tohoku J Exp Med 2006; 209: 149-57

・    G-protein beta3 subunit gene variant is unlikely to have a significant influence on serum uric acid level in Japanese workers.

     Suwazono Y, et al

 

Ornithol Sci 2006; 5: 139-143

・    Usefulness of avian buccal cells for molecular sexing.

     Arima H, et al

 

Int J Mol Med 2006; 17: 77-82

・    The -1438A/G polymorphism in the 5-hydroxytryptamine receptor 2A gene is related to hyperuricemia, increased gamma-glutamyl transpeptidase and decreased high-density lipoprotein cholesterol level in the Japanese population: a prospective cohort study over 5 years.

     Suwazono Y, et al

 

Arch Virol 2005; 150: 1927-31

・    Rotavirus antigenemia in children with encephalopathy accompanied by rotavirus gastroenteritis

     Nakagomi T, et al.

    

Thromb Res 2005; 115: 191-7

・    Factor XII Shizuoka, a novel mutation (Ala392Thr) identified and characterized in a patient with congenital coagulation factor XII deficiency

     Oguchi S, et al.

     

Hemoglobin 2005; 29: 1-10

・     Hb KOCHI [beta141(H19)Leu–>Val (g.1404C–>G); 144-146(HC1-3)Lys-Tyr-His –>0 (g.1413 A–>T)]: a new variant with increased oxygen affinity

     Miyazaki A, et al.

    

J Epidemiol 2005; 15: 203-10

・     Increased risk of obesity resulting from the interaction between high energy intake and the Trp64Arg polymorphism of the beta3-adrenergic receptor gene in healthy Japanese men.

     Miyaki K, et al

     

Br J Pharmacol 2005; 145: 818-28

・     Inhibition of the antigen-induced activation of rodent mast cells by putative Janus kinase 3 inhibitors WHI-P131 and WHI-P154 in a Janus kinase 3-independent manner.

     Watchara Linwong, et al

      

Blood Coagul Fibrinolysis 2004; 15: 367-73

・    Genetic analyses and expression studies identified a novel mutation (W486C) as a molecular basis of congenital coagulation factor XII deficiency

     Ishii K, et al.

    

Genes Chromosomes Cancer 2004; 39: 335-40

・    Protracted postnatal natural histories in childhood leukemia

     Maia AT, et al.

   

Obes Res 2004; 12: 4-8

・     Lack of association between human G-protein beta3 subunit variant and overweight in Japanese workers.

     Suwazono Y, et al

  

Drug Matab. Pharmacokin. 2004; 19: 303-7

・    Genotyping of single nucleotide polymorphism (SNPs) influencing drug response by competitive allele-specific short oligonucleotide hybridization (CASSOH) with immunochromatographic strip.

     Hiratsuka M, et al

   

J Vet Diagn Invest 2004; 16: 469-72

・    Rapid and simple mutation screening of GM1 gangliosidosis in Shiba dogs by direct amplification of deoxyribonucleic acid from various forms of canine whole-blood specimens.

     Yamato O, et al

 

      

Blood Coagul Fibrinolysis 2003; 14: 663-70

・    Association of the -159 C –> T polymorphism in the CD14 promoter with variations in serum lipoproteins in healthy subjects.

     Eilertsen KE, et al.

   

GENES, Chromosomes & Cancer 2003; 37: 36-43

・    Prenatal origin of TEL-AMLI-positive acute lymphoblastic leukemia in children born in California.

     McHale CM, et al

      

Arch Virol 2002; 147: 2187-95

・    Molecular characterization of serotype G2 and G3 human rotavirus strains that have an apparently identical electropherotype of the short RNA pattern

     Nakagomi T, et al.

   

BLOOD;2002;99:3801-5

・    In utero origin of t(8;21) AML1-ETO translocations in childhood acute myeloid leukemia

     Wiemels JL, et al

   

Proc. Natl. Acad. Sci. USA 2002; 99: 15101-6

・    Site-specific translocation and evidence of postnatal origin of the t(1;19) E2A-PBX1 fusion in childhood acute lymphoblastic leukemia.

     Wiemels JL, et al

  

Clin Lab; 2002; 48: 377-84

・    Various applications of direct PCR using blood samples

     Nishimura N, et al

     

Proceedings of the Seventh International Colloquium on Paratuberculosis; 2002; 267-9

・    Efficiency of polymerase chain reaction using a novel method of DNA preparation and amplification for detection of Mycobacterium avium subsp. paratuberculosis in bovine fecal samples

     Kojima K, et al

     

Arch Virol 2001; 146: 557-70

・    Direct evidence for genome segment reassortment between concurrently-circulating human rotavirus strains

     Watanabe M, et al.

   

Transfusion 2000; 40: 1081-7

・    Elimination of both cell-free and cell-associated HIV infectivity in plasma by a filtration/methylene blue photoinactivation system

     Abe H, et al.

     

Microbiol Immunol 2000; 44: 957-61

・    Apparent re-emergence of serotype G9 in 1995 among rotaviruses recovered from Japanese children hospitalized with acute gastroenteritis

     Oka T, et al

   

J Clin Microbiol 2000; 38: 2649-54

・    Major change in the predominant type of “Norwalk-like viruses” in outbreaks of acute nonbacterial gastroenteritis in Osaka city, Japan, between April 1996 and March 1999

     Iritani N, et al

   

Blood; 2000; 96:264-8

・    Detection of clonotypic IGH and TCR rearrangements in the neonatal blood spots of infants and children with B-cell precursor acute lymphoblastic leukemia

     Yagi T, et al

     

Ann Clin Biochem; 2000; 37: 674-80

・    Direct PCR from whole blood without DNA isolation

     Nishimura N, et al

     

Stroke 2000; 31: 2661-4

・    Polymorphism in the promoter of lipopolysaccharide receptor CD14 and ischemic cerebrovascular disease

     Ito D, et al

  

Stroke 2000; 31: 936-9

・     C242T polymorphism of NADPH oxidase p22 PHOX gene and ischemic cerebrovascular disease in the Japanese population

     Ito D, et al

  

Stroke 2000; 31: 493-7

・    Association between platelet glycoprotein Iba genotype and ischemic cerebrovascular disease

     Sonoda A, et al

产品列表
产品编号 产品名称 产品规格 产品等级 备注
604-21469 Ampdirect®Plus(INT) 1 mL×5(20 μL 体系500 次)
602-21421 Ampdirect®PlusBIOTAQ ™ HS DNA Polymerase 1 mL×5(20 μL 体系500 次) 250 units (5 units/μL)

Orion pH缓冲液 Cubitainer

简要描述:带有易于倾倒龙头的颜色编码的 Thermo Scientific Orion pH 缓冲液 Cubitainer 可容纳 5 加仑 (19 L) pH 缓冲液。

产品型号: 9104CB

所属分类:Orion

详情介绍

Thermo Scientific Orion pH缓冲液 Cubitainer

带有易于倾倒龙头的颜色编码的 Thermo Scientific Orion pH 缓冲液 Cubitainer 可容纳 5 加仑 (19 L) pH 缓冲液。

9104CB

Orion pH缓冲液 Cubitainer


9107CB

Orion pH缓冲液 Cubitainer


9110CB

Orion pH缓冲液 Cubitainer


备货品牌:

Thermo fisher (试剂耗材)、NUNC、Nalgene、QSP、Orion(奥立龙)、优特、Epredia(原Thermo 病理)、Labserv、Corning、Medicom麦迪康、 Xybio试剂、MKbio


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原Thermo病理产品Epredia:4951Pluse   p4981 超优 优加刀片3053835 3052835   包埋剂   包埋盒  

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SeraCare (KPL) 5460-0016 10X TRIS-GLYCINE-SDS BUFFER, 5 L         旧货号51-11-02 10X Tris-甘氨酸-SDS缓冲液 1 Bottle x 5 L

上海金畔生物科技有限公司提供SeraCare (KPL) 5460-0016 10X TRIS-GLYCINE-SDS BUFFER, 5 L         旧货号51-11-02 10X Tris-甘氨酸-SDS缓冲液 1 Bottle x 5 L,欢迎访问官网了解更多产品信息和订购

品牌 货号 产品名称 规格 价格

SeraCare (KPL) 5460-0016 10X TRIS-GLYCINE-SDS BUFFER, 5 L         旧货号51-11-02 10X Tris-甘氨酸-SDS缓冲液 1 Bottle x 5 L 3386.00

SeraCare (KPL) 5460-0015 10X TRIS-GLYCINE-SDS BUFFER, 1 L         旧货号51-11-01 10X Tris-甘氨酸-SDS缓冲液 1 Bottle x 1.0 L 1513.00

SeraCare (KPL) 5460-0012 10X TRIS-GLYCINE TRANSFER BUFFER, 5 L    旧货号51-10-02 10X Tris-甘氨酸转膜液 1 Bottle x 5 L 3386.00

SeraCare (KPL) 5460-0011 10X TRIS-GLYCINE TRANSFER BUFFER, 1 L    旧货号51-10-01 10X Tris-甘氨酸转膜液 1 Bottle x 1.0 L 1082.00

SeraCare (KPL) 5450-0012 SILVER ENHANCER KIT FOR MEMBRANE         旧货号50-22-02 WB 银离子增强剂 1 x 250 mL Silver Enhancer MB Solution A, 1x 250 mL, Silver Enhancer MB Solution B 5153.00

SeraCare (KPL) 5450-0011 HRP GOAT ANTI-MOUSE IGG (H+L), 1.0 ML    旧货号474-1806 HRP标记抗小鼠二抗 1 Vial x 1.0 mL 2027.00

SeraCare (KPL) 5450-0010 HRP GOAT ANTI-RABBIT IGG (H+L), 1.0 ML   旧货号474-1506 HRP标记抗兔二抗 1 Vial x 1.0 mL 2027.00

SeraCare (KPL) 5450-0009 HRP GOAT ANTI-HUMAN IGG (H+L), 1.0 ML    旧货号474-1006 HRP标记抗人二抗 1 Vial x 1.0 mL 2027.00

SeraCare (KPL) 5430-0055 PHOSPHAGLO AP SUBSTRATE, 100 ML          旧货号55-60-04 WB AP 底物 1 Bottle x 100 mL 5446.00

SeraCare (KPL) 5430-0054 PHOSPHAGLO AP SUBSTRATE, 30 ML           旧货号55-60-03 WB AP 底物 1 Bottle x 30 mL 2043.00

SeraCare (KPL) 5430-0053 PHOSPHAGLO RESERVE AP SUBSTRATE, 100 ML  旧货号55-60-02 WB AP 高灵敏底物 1 Bottle x 100 mL 8090.00

SeraCare (KPL) 5430-0052 PHOSPHAGLO RESERVE AP SUBSTRATE, 30 ML   旧货号55-60-01 WB AP 高灵敏底物 1 Bottle x 30 mL 2800.00

SeraCare (KPL) 5430-0051 LUMIGLO RESERVE™ CHEMI. SUBSTRATE, 50 ML 旧货号54-71-02 LumiGLO 高灵敏度化学发光底物 1 x 100mL 20X Wash Solution, 1 x 17 mL LumiGLO Reserve Chemiluminescent Substarte A, 1 x 34 mL LumiGLO Reserve Chemiluminescent Substrate B 6493.00

SeraCare (KPL) 5430-0050 LUMIGLO RESERVE CHEMI. SUBSTRATE,600 CM2 旧货号54-71-01 LumiGLO 高灵敏度化学发光底物 2 x 100 mL Wash Buffer, 1 x 10mL Solution A, & 1 x 20mL Solution B 4071.00

SeraCare (KPL) 5430-0049 LUMIGLO RESERVE CHEMI. SUBSTRATE,2400CM2 旧货号54-71-00 LumiGLO 高灵敏度化学发光底物 2 x 200 mL Wash Buffer, 1 x 40mL Solution A, & 1 x 80mL Solution B 10856.00

SeraCare (KPL) 5430-0042 LUMIGLO CHEMI. SUBSTRATE, 60 ML          旧货号54-61-02 LumiGLO 化学发光底物 2*30 mL 1220.00

SeraCare (KPL) 5430-0041 LUMIGLO CHEMI. SUBSTRATE KIT, 720 ML     旧货号54-61-01 LumiGLO 化学发光底物 720ml 6682.00

SeraCare (KPL) 5430-0040 LUMIGLO CHEMI. SUBSTRATE, 240 ML         旧货号54-61-00 LumiGLO 化学发光底物 1 X 120 mL LumiGLO Chemiluminescent Substrate A & 1 x 120 mL LumiGLO Chemiluminescent Substrate B 3109.00

SeraCare (KPL) 5420-0040 FIREPHOS™ MEMBRANE AP SUBS.100ml *DISC   100ml 1193.00

SeraCare (KPL) 5420-0038 BCIP/NBT AP SUBSTRATE, 100 ML kit        旧货号50-81-18 WB AP BCIP/NBT 1组分显色底物 1 Bottle x 100 mL 1014.00

Orion 独立使用袋装pH缓冲液

简要描述:使用 Thermo Scientific Orion 独立使用袋装 pH 缓冲液,确保用于 pH 值校准的标准品为新鲜、即用型。缓冲液采用颜色分类,便于使用。所有 Orion pH 缓冲液均可溯源于 NIST 标准参比物质。

产品型号: 910425

所属分类:Orion

详情介绍

Thermo Scientific Orion 独立使用袋装pH缓冲液

使用 Thermo Scientific Orion 独立使用袋装 pH 缓冲液,确保用于 pH 值校准的标准品为新鲜、即用型。缓冲液采用颜色分类,便于使用。所有 Orion pH 缓冲液均可溯源于 NIST 标准参比物质。

Orion 独立使用袋装pH缓冲液

备货品牌:

Thermo fisher (试剂耗材)、NUNC、Nalgene、QSP、Orion(奥立龙)、优特、Epredia(原Thermo 病理)、Labserv、Corning、Medicom麦迪康、 Xybio试剂、MKbio


优势产品:

Fisherbrand:载玻片 盖玻片 生物危害垃圾袋 肝素化非肝素化毛细管  塑料比色皿  细胞筛网等

原Thermo病理产品Epredia:4951Pluse   p4981 超优 优加刀片3053835 3052835   包埋剂   包埋盒  

Labserv耗材:细胞培养皿 血清移液管 细胞培养瓶 离心管 8联管 EP微量离心管 细胞培养板