Phytochelatin 2 (PC2)

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Phytochelatin 2 (PC2);

Phytochelatin 2 是一种植物螯合素，是一种重要的重金属结合肽。Phytochelatins 是一系列螯合金属、防止金属毒性和在金属内环境稳定中发挥作用的植物化合物。

Phytochelatin 2 (PC2)amp;;

Phytochelatin 2 (PC2) Chemical Structure

CAS No. : 95014-75-8

规格 是否有货
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生物活性

Phytochelatin 2, a short phytochelatin, is a key plant peptide binding heavy metals. Phytochelatins are a diverse set of plant compounds that chelate metals, protect against metal toxicity and function in metal homeostasis[1][2].

体外研究
(In Vitro)

Phytochelatin synthase (PCS) uses the substrates glutathione (GSH, γGlu-Cys-Gly) and a cadmium (Cd)-bound GSH (Cd∙GS2) to produce the shortest phytochelatin product (PC2, (γGlu-Cys)2-Gly) through a ping-pong mechanism[3].
Phytochelatins and their glutathione (GSH) precursor are thiol-rich peptides that play an important role in heavy metal detoxification in plants and microorganisms[4].

MCE has not independently confirmed the accuracy of these methods. They are for reference only.
分子量

539.60

Formula

C18H29N5O10S2
CAS 号

95014-75-8
Sequence

gamma;Glu-Cys-gamma;Glu-Cys-Gly
Sequence Shortening

(gamma;E-C)2-G
运输条件

Room temperature in continental US; may vary elsewhere.
储存方式

Please store the product under the recommended conditions in the Certificate of Analysis.
Solvent Solubility
In Vitro:;

H2O

Peptide Solubility and Storage Guidelines:

1.;;Calculate the length of the peptide.

2.;;Calculate the overall charge of the entire peptide according to the following table:

; Contents Assign value
Acidic amino acid Asp (D), Glu (E), and the C-terminal -COOH. -1
Basic amino acid Arg (R), Lys (K), His (H), and the N-terminal -NH2 +1
Neutral amino acid Gly (G), Ala (A), Leu (L), Ile (I), Val (V), Cys (C), Met (M), Thr (T), Ser (S), Phe (F), Tyr (Y), Trp (W), Pro (P), Asn (N), Gln (Q) 0

3.;;Recommended solution:

Overall charge of peptide Details
Negative (lt;0) 1.;;Try to dissolve the peptide in water first.
2.;;If water fails, add NH4OH (lt;50 μL).
3.;;If the peptide still does not dissolve, add DMSO (50-100 μL) to solubilize the peptide.
Positive (gt;0) 1.;;Try to dissolve the peptide in water first.
2.;;If water fails, try dissolving the peptide in a 10%-30% acetic acid solution.
3.;;If the peptide still does not dissolve, try dissolving the peptide in a small amount of DMSO.
Zero (=0) 1.;;Try to dissolve the peptide in organic solvent (acetonitrile, methanol, etc.) first.
2.;;For very hydrophobic peptides, try dissolving the peptide in a small amount of DMSO, and then dilute the solution with water to the desired concentration.
参考文献

  • [1]. phytochelatin, heavy, metals, chelate, homeostasis, glutathione, microorganisms, toxicity

    [2]. Dennis KK, et al. Phytochelatin database: a resource for phytochelatin complexes of nutritional and environmental metals. Database (Oxford). 2019;2019:baz083.

    [3]. Chia JC, et al. Tentative identification of the second substrate binding site in Arabidopsis phytochelatin synthase. PLoS One. 2013;8(12):e82675. Published 2013 Dec 5.

    [4]. Jacquart A, et al. Cd2+ and Pb2+ complexation by glutathione and the phytochelatins. Chem Biol Interact. 2017;267:2-10.