N-Formyl-Met-Leu-Phe-Lys(Synonyms: fMLFK) | Cytiva思拓凡-Whatman滤纸滤膜滤器

N-Formyl-Met-Leu-Phe-Lys;(Synonyms: fMLFK)

N-Formyl-Met-Leu-Phe-Lys 是一种多肽，为有效、选择性的 FPR1 激动剂，对 FPR1，FPR2 和 FPR2-D281^7.32G 的 EC₅₀ 值分别为 3.5 nM，6.7 μM 和 0.88 μM。

N-Formyl-Met-Leu-Phe-Lysamp;;(Synonyms: fMLFK)

N-Formyl-Met-Leu-Phe-Lys Chemical Structure

CAS No. : 67247-11-4

规格		是否有货
100 mg	;	询价	;
250 mg	;	询价	;
500 mg	;	询价	;

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生物活性

N-Formyl-Met-Leu-Phe-Lys (fMLFK) is a peptide, acts as a potent and selective agonist of FPR1, with EC₅₀s of 3.5 nM, 6.7 μM and 0.88 μM for FPR1, FPR2 and FPR2-D281^7.32G, respectively^[1].

IC₅₀ Target

EC50: 3.5 nM (FPR1), 6.7 μM (FPR2), 0.88 μM (FPR2-D281^7.32G)^[1]

分子量

565.73

Formula

C₂₇H₄₃N₅O₆S

CAS 号

67247-11-4

Sequence

Formyl-Met-Leu-Phe-Lys

Sequence Shortening

Formyl-MLFK

运输条件

Room temperature in continental US; may vary elsewhere.

储存方式

Please store the product under the recommended conditions in the Certificate of Analysis.

Solvent Solubility

In Vitro:;

H₂O

Peptide Solubility and Storage Guidelines:

1.;;Calculate the length of the peptide.

2.;;Calculate the overall charge of the entire peptide according to the following table:

;	Contents	Assign value
Acidic amino acid	Asp (D), Glu (E), and the C-terminal -COOH.	-1
Basic amino acid	Arg (R), Lys (K), His (H), and the N-terminal -NH₂	+1
Neutral amino acid	Gly (G), Ala (A), Leu (L), Ile (I), Val (V), Cys (C), Met (M), Thr (T), Ser (S), Phe (F), Tyr (Y), Trp (W), Pro (P), Asn (N), Gln (Q)	0

3.;;Recommended solution:

Overall charge of peptide	Details
Negative (lt;0)	1.;;Try to dissolve the peptide in water first. 2.;;If water fails, add NH₄OH (lt;50 μL). 3.;;If the peptide still does not dissolve, add DMSO (50-100 μL) to solubilize the peptide.
Positive (gt;0)	1.;;Try to dissolve the peptide in water first. 2.;;If water fails, try dissolving the peptide in a 10%-30% acetic acid solution. 3.;;If the peptide still does not dissolve, try dissolving the peptide in a small amount of DMSO.
Zero (=0)	1.;;Try to dissolve the peptide in organic solvent (acetonitrile, methanol, etc.) first. 2.;;For very hydrophobic peptides, try dissolving the peptide in a small amount of DMSO, and then dilute the solution with water to the desired concentration.

参考文献

[1]. He HQ, et al. Structural determinants for the interaction of formyl peptide receptor 2 with peptide ligands. J Biol Chem. 2014 Jan 24;289(4):2295-306.