Bak BH3 | Cytiva思拓凡-Whatman滤纸滤膜滤器

Bak BH3;

Bak BH3 是一种多肽，源于 Bak 蛋白的 BH3 结构域，在细胞中，能够抑制 Bcl-xL 的活性。

Bak BH3 Chemical Structure

规格	价格	是否有货
1 mg	￥1100	询问价格货期
5 mg	￥3300	询问价格货期
10 mg	￥5500	询问价格货期

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生物活性

Bak BH3 is derived from the BH3 domain of Bak, can antagonize the function of Bcl-xL in cells.

IC₅₀ Target^[1]

Bcl-xL

;

体外研究
(In Vitro)

Bak BH3 peptide antagonize the protective effects of microinjected Bcl-xL in α-Fas-treated HeLa cells, whereas a mutant Bak BH3 peptide that no longer binds Bcl-xL is inactive^[1].

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

分子量

1724.90

Formula

C₇₂H₁₂₅N₂₅O₂₄

Sequence

Gly-Gln-Val-Gly-Arg-Gln-Leu-Ala-Ile-Ile-Gly-Asp-Asp-Ile-Asn-Arg

Sequence Shortening

GQVGRQLAIIGDDINR

运输条件

Room temperature in continental US; may vary elsewhere.

储存方式

Please store the product under the recommended conditions in the Certificate of Analysis.

参考文献

[1]. Holinger EP, et al. Bak BH3 peptides antagonize Bcl-xL function and induce apoptosis through cytochrome c-independent activation of caspases. J Biol Chem. 1999 May 7;274(19):13298-304.

Cell Assay ^[1]	Cells are plated in complete DMEM in 96-well tissue culture plates at 4×10³/well. After 24 h, cells are washed with PBS and treated with peptides (50 μM) in SF-DMEM. Cell viability is determined by staining unfixed cells with calcein AM/ethidium homodimer, followed by microscopic analysis of cell staining and cellular morphology on a Nikon Diaphot 300 inverted microscope equipped with a fluorescence module. MCE has not independently confirmed the accuracy of these methods. They are for reference only.
参考文献	[1]. Holinger EP, et al. Bak BH3 peptides antagonize Bcl-xL function and induce apoptosis through cytochrome c-independent activation of caspases. J Biol Chem. 1999 May 7;274(19):13298-304.

Cell Assay
^[1]

Cells are plated in complete DMEM in 96-well tissue culture plates at 4×10³/well. After 24 h, cells are washed with PBS and treated with peptides (50 μM) in SF-DMEM. Cell viability is determined by staining unfixed cells with calcein AM/ethidium homodimer, followed by microscopic analysis of cell staining and cellular morphology on a Nikon Diaphot 300 inverted microscope equipped with a fluorescence module.

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

参考文献

[1]. Holinger EP, et al. Bak BH3 peptides antagonize Bcl-xL function and induce apoptosis through cytochrome c-independent activation of caspases. J Biol Chem. 1999 May 7;274(19):13298-304.