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Products > Optimize Reagents > Optimize – Buffers > Tris Buffer

Tris Buffer

Hampton蛋白结晶试剂盒Tris Buffer/HR2-589/HR2-725

CAT NO

HR2-589

NAME

1.0 M Tris

DESCRIPTION

100 mL

PRICE

$60.00

CAT NO

HR2-725

NAME

1.0 M Tris pH 8.5

DESCRIPTION

100 mL

PRICE

$60.00

应用

  • Crystallization grade Tris for formulating screens or for optimization
  • 结晶级 Tris 用于配制筛选或优化

特征

  • Sterile filtered
  • Formulated in Type 1+ ultrapure water: 18.2 megaohm-cm resistivity at 25°C, < 5 ppb Total Organic Carbon, bacteria free (<1 Bacteria (CFU/ml)), pyrogen free (<0.03 Endotoxin (EU/ml)), RNase-free (< 0.01 ng/mL) and DNase-free (< 4 pg/µL)
  • 无菌过滤
    在 1+ 型超纯水中配制:25°C 时电阻率为 18.2 兆欧厘米,总有机碳 < 5 ppb,无细菌(<1 细菌 (CFU/ml)),无热原(<0.03 内毒素 (EU/ml)) , 无 RNase (< 0.01 ng/mL) 和无 DNase (< 4 pg/µL)

描述Tris

Synonyms: Trizma base or 2-Amino-2-(hydroxymethyl)-1,3-propanediol or THAM or Tris base or Tris(hydroxymethyl)aminomethane or Trometamol
C4H11NO3
NH2C(CH2OH)3
Mr 121.14
CAS Number [77-86-1]
EC Number 201-064-4
Beilstein Registry Number 741883
Merck 14,9772
RTECS TY2900000
MDL Number MFCD00004679
PubChem Substance ID 24889854
Purity 99.8 – 100.1%
pKa = 8.1 at 25°C

Useful pH range of Tris: 7.0 – 9.0

Titrate HR2-589 to the desired pH using HCl
HR2-589 Measured pH Range: 10.9 – 11.1 at 25°C
HR2-589 Measured Conductivity Range: 193.3 – 236.7 µS/cm at 25°C
HR2-589 Measured Refractive Index: 1.34991 – 1.35058 at 20°C

HR2-725 is titrated to pH 8.5 using HCl
HR2-725 Measured Conductivity Range: 18.1 – 22.7 mS/cm at 25°C
HR2-725 Measured Refractive Index: 1.35310 – 1.35369 at 20°C

同义词: Trizma 碱或 2-Amino-2-(羟甲基)-1,3-丙二醇或 THAM 或 Tris 碱或 Tris(羟甲基)氨基甲烷或氨丁三醇
C4H11NO3
NH2C(CH2OH)3
121.14 先生
CAS 编号 [77-86-1]
欧盟编号 201-064-4
贝尔斯坦注册号 741883
默克 14,9772
RTECS TY2900000
MDL 编号 MFCD00004679
PubChem 物质 ID 24889854
纯度 99.8 – 100.1%
pKa = 8.1 在 25°C

Tris 的有用 pH 值范围:7.0 – 9.0

使用 HCl 将 HR2-589 滴定至所需的 pH 值
HR2-589 测得的 pH 范围:25°C 时为 10.9 – 11.1
HR2-589 测量的电导率范围:25°C 时为 193.3 – 236.7 µS/cm
HR2-589 实测折射率:20°C 时为 1.34991 – 1.35058

使用 HCl 将 HR2-725 滴定至 pH 8.5
HR2-725 测量的电导率范围:25°C 时为 18.1 – 22.7 mS/cm
HR2-725 实测折射率:20°C 时为 1.35310 – 1.35369

Insoluble Matter 0.005% max
DNase None Detected
RNase None Detected
Protease None Detected
Heavy Metals 5 ppm max
As  ≤ 5 ppm max
Ca  ≤ 5 ppm max
Cu  ≤ 5 ppm max
Fe  ≤ 5 ppm max
Pb  ≤ 5 ppm max
Mg ≤ 5 ppm max

Trizma is a registered trademark of Sigma-Aldrich Co.

支持材料

Hampton蛋白结晶试剂盒Tris Buffer/HR2-589/HR2-725 HR2-589 Tris SDSHampton蛋白结晶试剂盒Tris Buffer/HR2-589/HR2-725 HR2-725 Tris pH 8.5 SDSHampton蛋白结晶试剂盒Tris Buffer/HR2-589/HR2-725 Titration Table

相关项目

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    个人股票期权 Tris 试剂

参考

1. Purification and crystallization of Pseudomonas aeruginosa chloramphenicol acetyltransferase. Tian, Y; Beaman, TW; Roderick, SL; Proteins 28, 298- 300, 1997.

1. 铜绿假单胞菌氯霉素乙酰转移酶的纯化与结晶。 田,Y; 比曼,台湾; 罗德里克,SL; 蛋白质 28, 298-300, 1997。