美国密理博Millipore Amicon Ultra超滤离心管
简要描述:
Amicon® Ultra离心超滤管
• 高回收率 Ultracel 再生纤维素膜具有一系列截留分子量
• 高回收率> 90%
• 垂直结构的膜减少了浓差极化,加快离心速度,快达 5 分钟
• 热密封膜将下游溶出物污染可能性降到zui低
• 100% 完整性检测确保性能可靠
• 透明外壳和体积刻度方便样品察看
• 直接吸取样品减少处理步骤
• 高浓缩倍数:80–100 倍
应用
• 生物样品浓缩:包括抗原、、酶、核酸或微生物
• 组织培养提取物或细胞裂解液中大分子组分的纯化
• 对稀释或从柱洗脱液预先纯化的蛋白质进行浓缩
• 脱盐和缓冲液更换
典型蛋白质回收
膜NMWL,Da |
蛋白质溶质 |
蛋白质截留物体积,mL |
蛋白质截留物,回收率,% |
3,000 |
细胞色素 c,12,400 Da(0.25mg/mL) |
0.19 |
98.7 |
10,000 |
细胞色素 c,12,400 Da(0.25mg/mL) |
0.20 |
95 |
30,000 |
BSA,67,000 Da(1mg/mL) |
0.20 |
95 |
50,000 |
BSA,67,000 Da(1mg/mL) |
0.08 |
94 |
100,000 |
IgG,156,000 Da(1mg/mL) |
0.34 |
91 |
4 mL 起始量,固定角度转子,7,500 x g,25°C。离心时间:3K (30 min);10 和 30K (10 min);50K (5 min);100K (15 min) |
Amicon Ultra-0.5系列
货号 |
产品 |
NMWL,kDa |
包装数量 |
UFC500308 |
Amicon Ultra-0.5, Ultracel-3 Membrane, 3 kDa |
3 |
8 |
UFC500324 |
Amicon Ultra-0.5, Ultracel-3 Membrane, 3 kDa |
3 |
24 |
UFC500396 |
Amicon Ultra-0.5, Ultracel-3 Membrane, 3 kDa |
3 |
96 |
UFC5003BK |
Amicon Ultra-0.5, Ultracel-3 Membrane, 3 kDa |
3 |
500 |
UFC501008 |
Amicon Ultra-0.5, Ultracel-10 Membrane, 10 kDa |
10 |
8 |
UFC501024 |
Amicon Ultra-0.5, Ultracel-10 Membrane, 10 kDa |
10 |
24 |
UFC501096 |
Amicon Ultra-0.5, Ultracel-10 Membrane, 10 kDa |
10 |
96 |
UFC5010BK |
Amicon Ultra-0.5, Ultracel-10 Membrane, 10 kDa |
10 |
500 |
UFC503008 |
Amicon Ultra-0.5, Ultracel-30 Membrane, 30 kDa |
30 |
8 |
UFC503024 |
Amicon Ultra-0.5, Ultracel-30 Membrane, 30 kDa |
30 |
24 |
UFC503096 |
Amicon Ultra-0.5, Ultracel-30 Membrane, 30 kDa |
30 |
96 |
UFC5030BK |
Amicon Ultra-0.5, Ultracel-30 Membrane, 30 kDa |
30 |
500 |
UFC505008 |
Amicon Ultra-0.5, Ultracel-50 Membrane, 50 kDa |
50 |
8 |
UFC505024 |
Amicon Ultra-0.5, Ultracel-50 Membrane, 50 kDa |
50 |
24 |
UFC505096 |
Amicon Ultra-0.5, Ultracel-50 Membrane, 50 kDa |
50 |
96 |
UFC5050BK |
Amicon Ultra-0.5, Ultracel-50 Membrane, 50 kDa |
50 |
500 |
UFC510008 |
Amicon Ultra-0.5, Ultracel-100 Membrane, 100 kDa |
100 |
8 |
UFC510024 |
Amicon Ultra-0.5, Ultracel-100 Membrane, 100 kDa |
100 |
24 |
UFC510096 |
Amicon Ultra-0.5, Ultracel-100 Membrane, 100 kDa |
100 |
96 |
UFC5100BK |
Amicon Ultra-0.5, Ultracel-100 Membrane, 100 kDa |
100 |
500 |