有效的新型流式细胞仪检测和抗体　　

　　流式细胞仪是一个功能强大的工具，可使不同目标的表达进行单细胞分析。使用不同的荧光和探针可以收集到有意义的信息和完整的细胞特征。

●    CELLESTIAL^® Fluorescent Assays

      细胞存活、增殖和死亡分析的细胞基础检测

●    FLOWSCRIPT^® HPV E6/E7 Assay

      HPV癌蛋白的 mRNA单细胞检测

●    抗体

      超过600种抗体经过特异性验证，可用于流式细胞仪

CELLESTIAL® Fluorescent Assay 直接标记自噬泡

　　HeLa细胞进行饥饿，恢复，用CYTO-ID^®

　　Autophagy Dye标记

应用笔记

治疗性蛋白质制剂imagestreamx^® 流式细胞仪成像粒度分析和proteostat^® 蛋白聚集检测

Particle analysis of therapeutic protein formulations with ImageStreamX^® Imaging Flow Cytometry and the ProteoStat^® Protein Aggregation Assay

Christine Probst, Brian Hall, and David Basiji.
Amnis part of EMD Millipore, 645 Elliott Ave W, Suite 100, Seattle, WA 98119, USA
 

Abstract

The formation of protein aggregates (PA) and other subvisible particles within protein-based drugs is a major concern for the pharmaceutical industry because they can impact drug activity or cause unwanted immunogenicity in patients. It is critical to monitor the presence of these particulates to ensure the safety and efficacy of these drugs, especially since their formation can be highly sensitive to production and storage conditions. No currently accepted industry method meets requirements to simultaneously measure size, enumerate, and classify particles within the critical 1-100 um size range, thus illustrating the need to develop new approaches for this application. In this study we evaluate the potential for the Amnis ImageStreamX MKII^® (ISX) imaging flow cytometer, using the PROTEOSTAT^® Protein Aggregation Assay to address these criteria and enable comprehensive particulate characterization for therapeutic protein formulations.