Alvetex™ 3D细胞培养系统
◆Alvetex 孔板型
● 适用于在支架上半部或上部分层进行3D培养 ● 只能从支架顶部向细胞供料 ● 适用于需要限制细胞渗透的情况 ● 适用于昂贵细胞,减少细胞使用数量(如细胞转染) ● 支持高通量3D培养 |
◆Alvetex 插入式培养器型
● 可同时从上方和下方向细胞供料 ● 长期3D培养的理想选择 ● 细胞在气/液界面上生长 ● 可轻松地将3D培养物转移至新板 ● 支持共培养研究(2D+3D或3D+3D) |
◆Alvetex 插入式培养器支架
● 减少培养基更换频率,最多可更换 95 mL 培养基 ● 支持单个细胞的3D培养 ● 非常适合用于持续长达数周的长期3D培养实验 ● 如有需要,可使用磁力搅拌器促进培养基循环 |
◆Alvetex 灌注板
● 可交替互用的Luerlock设计,能适应各种大小的管径 ● 组织碎片、在Alvetex插入式培养器中的3D培养物和2D细胞可保存在灌注板中(对于2D细胞,塑料器皿可 能需要用适当的细胞培养试剂进行涂抹,以促进细胞粘附) ● 不同孔间单向流动,使得可根据旁分泌因子的释放来研究在不同孔生长的细胞群间的相互作用 |
Alvetex是什么?
Alvetex是一种多孔聚苯乙烯膜,可用于3D细胞培养。其90%的结构为惰性、一致的多孔结构,为细胞生长提供了最大的空间。
细胞在Alvetex支架内(或在Alvetex顶层)的3D空间中进行生长和分裂时,可保持其自然形状并以类似在组织中正常生长的方式形成复杂的相互作用。细胞可能会沉积细胞外基质,这通常会导致“微板”组织结构的形成。
一般哺乳动物的细胞大小约为10-25 μm,很少与另一个细胞的距离会超过0-50 μm,或通过毛细血管时与营养源的距离超过100-200 μm。Alvetex 3D细胞培养与传统的2D模型系统相比,可重现原生组织内细胞所处的复杂细胞组织和环境,能够更准确地研究细胞行为和功能。
Alvetex与广泛的标准分子、细胞和组织学技术兼容,并且可提供多种类型产品,包括多孔板和内置型培养皿。
图:通过各种技术可视化细胞在Alvetex中生长的示例。 A.细胞在Alvetex支架上生长的扫描电子显微照片。 B.鼠角质细胞在Alvetex支架中的生长;固定、包埋、石蜡切片、染色后通过明视野显微镜观察。 C.肝癌HepG2细胞在Alvetex支架上的生长。三重荧光染色后通过共聚焦显微镜观察。 D.Caco-2细胞在Alvetex顶层的3D生长情况。 |
◆产品列表
Alvetex 孔板
产品编号 | 产品名称 | 包装 |
AVP002-2 | Alvetex Scaffold 12 well plate×2 Alvetex Scaffold 12 孔板×2 |
1 set |
AVP002-10 | Alvetex Scaffold 12 well plate×10 Alvetex Scaffold 12 孔板×10 |
1 set |
AVP002-80 | Alvetex Scaffold 12 well plate×80 Alvetex Scaffold 12 孔板×80 |
1 set |
AVP006-2 | Alvetex Scaffold 24 well plate×2 Alvetex Scaffold 24 孔板×2 |
1 set |
AVP006-10 | Alvetex Scaffold 24 well plate×10 Alvetex Scaffold 24 孔板×10 |
1 set |
AVP006-80 | Alvetex Scaffold 24 well plate×80 Alvetex Scaffold 24 孔板×80 |
1 set |
AVP009-2 | Alvetex Scaffold 96 well plate×2 Alvetex Scaffold 96 孔板×2 |
1 set |
AVP009-10 | Alvetex Scaffold 96 well plate×10 Alvetex Scaffold 96 孔板×10 |
1 set |
AVP009-80 | Alvetex Scaffold 96 well plate×80 Alvetex Scaffold 96 孔板×80 |
1 set |
Alvetex 孔内置型
产品编号 | 产品名称 | 包装 |
AVP004-12 | Alvetex Scaffold 6 well insert×12 Alvetex Scaffold 6 孔内置型培养板×12 |
1 set |
AVP004-48 | Alvetex Scaffold 6 well insert×48 Alvetex Scaffold 6 孔内置型培养板×48 |
1 set |
AVP004-96 | Alvetex Scaffold 6 well insert×96 Alvetex Scaffold 6 孔内置型培养板×96 |
1 set |
AVP005-12 | Alvetex Scaffold 12 well insert×12 Alvetex Scaffold 12 孔内置型培养板×12 |
1 set |
AVP005-48 | Alvetex Scaffold 12 well insert×48 Alvetex Scaffold 12 孔内置型培养板×48 |
1 set |
AVP005-96 | Alvetex Scaffold 12 well insert×96 Alvetex Scaffold 12 孔内置型培养板×96 |
1 set |
AVP012-12 | Alvetex Scaffold 24 well insert×12 AlvetexScaffold 24 孔内置型培养板×12 |
1 set |
AVP012-48 | Alvetex Scaffold 24 well insert×48 AlvetexScaffold 24 孔内置型培养板×48 |
1 set |
AVP012-96 | Alvetex Scaffold 24 well insert×96 AlvetexScaffold 24 孔内置型培养板×96 |
1 set |
STP004-12 | Alvetex Strata 6 well insert×12 Alvetex Strata 6-孔内置型培养板×12 |
1 set |
STP004-48 | Alvetex Strata 6 well insert×48 Alvetex Strata 6-孔内置型培养板×48 |
1 set |
STP004-96 | Alvetex Strata 6 well insert×96 Alvetex Strata 6-孔内置型培养板×96 |
1 set |
STP005-12 | Alvetex Strata 12 well insert×12 Alvetex Strata 12-孔内置型培养板×12 |
1 set |
STP005-48 | Alvetex Strata 12 well insert×48 Alvetex Strata 12-孔内置型培养板×48 |
1 set |
STP005-96 | Alvetex Strata 12 well insert×96 Alvetex Strata 12-孔内置型培养板×96 |
1 set |
Alvetex 孔内置型支架
产品编号 | 产品名称 | 包装 |
AVP015-2 | Alvetex Well insert holder x 2 and deep Petri-dish×2 内置型支架×2/培养皿×2 |
1 set |
AVP015-10 | Alvetex Well insert holder x 10 and deep Petri-dish×10 内置型支架×10 /培养皿×10 |
1 set |
Alvetex 灌注板
产品编号 | 产品名称 | 包装 |
AVP011-2 | Alvetex Perfusion plate×2 灌注培养板 |
1 set |
AVP011-10 | Alvetex Perfusion plate×10 灌注培养板 |
1 set |
Alvetex Kits
产品编号 | 产品名称 | 包装 |
AVP-KIT-1 | Alvetex Scaffold Plate Starter Kit 平板套装 |
1 set |
AVP-KIT-2 | Alvetex Kit Scaffold Well Insert Starter Kit 内置型培养板套装 |
1 set |
STP-KIT-2 | Alvetex Kit Strata Well Insert Starter Kit | 1 set |
AVP-KIT-3 |
Alvetex Perfusion Plate Kit (Perfusion plate x 2 and Alvetex Scaffold 6 well insert×12) 灌注培养板*2/6 孔内置皿*12 |
1 set |
AVP-KIT-4 |
Alvetex Perfusion Plate Kit (Perfusion plate x 2 and Alvetex Scaffold 12 well insert×12) 灌注培养板*2/12 孔内置皿*12 |
1 set |
AVP-KIT-5 |
Alvetex Perfusion Plate Kit (Perfusion plate x 5 and Alvetex Scaffold 6 well insert×48) 灌注培养板*5/6 孔内置皿*48 |
1 set |
AVP-KIT-6 |
Alvetex Perfusion Plate Kit (Perfusion plate x 5 and Alvetex Scaffold 12 well insert×48) 灌注培养板*5/12 孔内置皿*48 |
1 set |