LBIS Human IL-7 ELISA Kit
短时间/微量样品/高灵敏度检测人血清(血浆)中的IL-7
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LBIS Human IL-7 ELISA Kit
短时间/微量样品/高灵敏度检测人血清(血浆)中的IL-7
FUJIFILM Wako Shibayagi Corporation的LBIS Cytokine ELISA Kit系列推出了新产品Human IL-7。对于现有产品因缺乏灵敏度而无法检测的正常样品和低浓度样品,新产品可以高灵敏度且重复性良好地进行检测。
IL-7主要由骨髓、胸腺、淋巴器官或组织的基质细胞等非造血细胞产生,是诱导干细胞分化为免疫细胞的细胞因子。成熟体由152个氨基酸组成。CD4+、CD8+两种细胞的增殖和发生、促进抗病毒活性,还与类风湿关节炎、慢性结肠炎的产生以及与嗜酸性粒细胞活性引起的哮喘有关。另外,作为抗癌药和免疫检查点相关药物联合治疗中的助推因子,高灵敏度IL-7的检测,在NK细胞、B细胞和T细胞的增强作用物质的探索和研究中也备受期待。
◆特点
● 不适用于卡塔赫纳生物安全议定书(不含杆状病毒)
● 微量样品的检测
● 可短时间检测(总反应时间:2 h 50 min)
● 使用环保防腐剂
● 准确性和重复性高
◆性能
样品 |
人血清/血浆(肝素/EDTA) |
校准曲线范围 |
1.50~236 pg/mL |
Assay内差异(5次检测,2个样品) |
平均C.V. 值:不足15% |
Assay间差异(3次检测,3个样品,4天) |
平均C.V. 值:不足15% |
◆数据
■ 人样品检测(例)
Sample No. |
检测值 |
Sample No. |
检测值 |
1 |
3.9 |
7 |
3.78 |
2 |
2.44 |
8 |
11 |
3 |
7.59 |
9 |
40 |
4 |
6.21 |
10 |
10 |
5 |
9.36 |
11 |
3.5 |
6 |
5.35 |
人正常血清,2重检测,单位:pg/mL
◆试剂盒组成
● 抗体固定96孔板···········································96 wells(8×12)×1张
● 人IL-7标准品······················································································1瓶
● 缓冲液···················································································60 mL×1瓶
● 生物素结合抗IL-7抗体······································································1瓶
● 过氧化物酶·亲和素结合物···············································150 μL×1瓶
● 显色液(TMB)··································································12 mL×1瓶
● 反应终止液(1 mol/L H2SO4)·····································12 mL×1瓶
● 浓缩清洗液(10×)···························································100 mL×1瓶
◆产品列表
产品编号 |
厂家编号 |
产品名称 |
包装 |
637-50441 |
AKH-IL7 |
LBIS Human IL-7 ELISA Kit |
96 tests |
相关产品
产品编号 |
厂家编号 |
产品名称 |
标准曲线范围(pg/mL) |
包装 |
635-42311 |
AKH-IL6 |
LBIS Human IL-6 ELISA Kit |
1.16~500 |
96 tests |
632-42321 |
AKH-IL8 |
LBIS Human IL-8 (CXCL8)ELISA Kit |
0.686~500 |
96 tests |
639-42331 |
AKHTNFA |
LBIS Human TNF-α ELISA Kit |
2.05~500 |
96 tests |
631-40831 |
AKHVEGF |
LBIS Human VEGF ELISA Kit |
1.10~800 |
96 tests |
631-47891 |
AKHIFNG |
LBIS Human IFN-γ ELISA Kit |
0.768~75.0 |
96 tests |