BAMBANKER^® 是一种无血清细胞冻存液。可在 -80℃ 长期保存细胞（肿瘤细胞和常规细胞）。

P3U1

（小鼠骨髓瘤细胞系）

K562

（人白血病细胞系）

human γδT cells

（人γδT细胞）

 Daudi

（人 B细胞系）

PC12

（大鼠源肾上腺嗜铬细胞瘤）

human B cell line 

（人B细胞系）

OKT4

（小鼠杂交瘤细胞）

B细胞系

(猴）

3T3‐L1（小鼠前脂肪细胞系）

A431（人扁平上皮癌细胞系）

BAEC（牛主动脉血管内皮细胞系）

Balb/3T3（小鼠成纤维细胞系）

C2C12（小鼠骨骼肌细胞系）

 Daudi（人B细胞系）

ECV304（人脐静脉内皮细胞系）

H295R（肾上腺皮质细胞）

HEK293（人胚胎肾细胞系）

HEK293T（人胚胎肾细胞系）

HeLa（人子宫颈癌细胞系）

HeLa S3（人子宫颈癌细胞系）

HepG2（人肝癌细胞系）

HFF（人正常成纤维细胞系）

Huh7（人肝癌细胞系）

Jurkat（人白血病T细胞系）

K562（人慢性骨髓性白血病细胞系）

KATOIII （人胃癌上皮细胞系）

KLM‐1（人胰腺癌细胞系）

MDCK（犬肾小管上皮细胞系）

MEF（小鼠胚胎成纤维细胞）

NIH3T3（小鼠胚胎皮肤细胞）

OKT4（小鼠杂交瘤细胞）

OP9（小鼠骨髓基质细胞）

P3U1（小鼠骨髓瘤细胞系）

PANC‐1（人胰腺癌细胞系）

PC12（大鼠源肾上腺嗜铬细胞瘤）

RPE（人视网膜上皮细胞系）

SNL（小鼠胚胎成纤维细胞）

TSU‐Pr1（人前列腺癌细胞系）

Vero（非洲绿猴肾细胞系）

human γδT cells （人γδT细胞）

human B cell line （人B细胞系）

HS-RMS-2

（多形性横纹肌肉瘤细胞系）

LNCaP clone FGC

（人前列腺癌细胞）

MEF-BL/6-1

（小鼠胚胎成纤维细胞）

猴B细胞系

人外周血活化淋巴细胞

永生化人肌肉细胞

小鼠脾脏活化淋巴细胞

小鼠ES细胞系

人胃上皮细胞

BAMBANKER^® 无血清细胞冻存液

Wako BAMBANKER冻存液（新手册）

细胞种类列举

BAMBANKER细胞冻存液

–冻存解冻步骤说明

（终端）.pdf

１.

Q：为什么我使用了 BAMBANKER^® 来保存细胞，但是存活率依然不高？

A：请冻存前确保细胞处于生长对数期，并且冻存时细胞数目控制在 5×10⁵~1×10⁷/mL 冻存液。

2.

Q：我们实验室已经有固定的冻存程序了，换了你们的 BAMBANKER^® 可以还继续用原来程序降温的方法冻存吗？

A：虽然本产品可以无需程序降温冻存细胞，但如果通过程序降温盒等适当控制了温度下降的速度，效果更佳。

3.

Q：哪些细胞株（系）适合使用BAMBANKER^® 来进行细胞冷冻保存？

A：几乎所有细胞株（系）都可以使用 BAMBANKER^® 进行冻存。对于较为宝贵的 ES/iPS 细胞的保存尤其适用。官网上所列举的细胞系均已经过测试验证。

但也并不排除可能有某些细胞株是不适合使用 BAMBANER^® 来进行冻存的，用户在没有确认是否可使用时，建议在进行正式细胞冻存之前先进行预实验。

4.

Q：无血清冻存液相比传统含血清冻存液有什么优势？

A：无血清冻存液因不含有动物血清，质量更稳定，批间差小；同时未知生物成分或感染性物质污染细胞的几率也极低，尤其对于 ES/iPS 细胞等有可能用于再生医疗的细胞安全得以严格保障；可以直接冻存无血清培养的细胞，免去无血清再驯化的步骤；另外 BAMBANKER^® 无血清细胞冻存液不需要像传统的血清冻存液需要程序降温，减少了用户的繁琐操作，节省了时间。

5.

Q：BAMBANKER^® 在冷冻保存细胞的过程中起什么作用？

A：BAMBANKER^® 无血清细胞冻存液使用了 DMSO 等作为保护剂，在冻存细胞时能以１℃/min 左右的温度下降而逐渐冻结，在此过程中，细胞内的水分子被置换成冻结保护剂，抑制胞内和细胞周边的冰晶的形成，防止细胞膜和细胞器结构损伤，防止蛋白变质。

6.

Q：未使用的BAMBANKER^® 无血清细胞冻存液应该如何保存？

A：2-10℃ 避光保存。开封后尽快使用。请注意保质期为自生产日期起 24 个月。

7.

Q：BAMBANKER^® 能否使用于医疗领域?

A：BAMBANKER^® 仅供科研使用，不能使用于人体或医疗领域。

[1]

Zhang C., Seo J., Nakamura T.   (2018) Cellular Approaches in Investigating Argonaute2-Dependent RNA   Silencing. In: Okamura K., Nakanishi K. (eds) Argonaute Proteins. Methods in   Molecular Biology, vol 1680. Humana Press, New York, NY.

[2]

Sharma, A., M¨ucke, M., &   Seidman, C. E. (2018). Human induced pluripotent stem cell production and   expansion from blood using a non-integrating viral reprogramming vector.   Current Protocols in Molecular Biology,122, e58. doi: 10.1002/cpmb.58.

[3]

Souta Motoike, Mikihito Kajiya,   Nao Komatsu, et al. Cryopreserved clumps of mesenchymal stem   cell/extracellular matrix complexes retain osteogenic capacity and induce   bone regeneration. Stem Cell Res Ther. 2018; 9: 73. Published online 2018 Mar   21. doi: 10.1186/s13287-018-0826-0.

[4]

Konuma T1, Kohara C1, Watanabe   E2, et al. Monocyte subsets and their phenotypes during treatment with   BCR-ABL1 tyrosine kinase inhibitors for Philadelphia chromosome-positive   leukemia. Hematol Oncol. 2018 Apr;36(2):451-456. doi: 10.1002/hon.2497. Epub   2018 Feb 12.

[5]

Srijaya Thekkeparambil   Chandrabose, Sandhya Sriram, et al. Amenable epigenetic traits of dental pulp   stem cells underlie high capability of xeno-free episomal reprogramming.

Stem Cell Research & Therapy 2018 9:68.

[6]

Evans, Michael A. et al.   "Macrophage-Mediated Delivery of Light Activated Nitric Oxide Prodrugs with   Spatial, Temporal and Concentration Control." Chemical Science (2018): n.   pag. Web. doi:10.1039/C8SC00015H.

[7]

Jauregui, C.; Yoganarasimha, S.;   Madurantakam, P. Mesenchymal Stem Cells Derived from Healthy and Diseased   Human Gingiva Support Osteogenesis on Electrospun Polycaprolactone Scaffolds.   Bioengineering 2018, 5, 8.

[8]

Khamaikawin, Wannisa et al.   Modeling Anti-HIV-1 HSPC-Based Gene Therapy in Humanized Mice Previously   Infected with HIV-1. Molecular Therapy – Methods & Clinical Development ,   Volume 9,23-32.

[9]

Masako Okumura, Toyoaki Natsume,   Masato T Kanemaki, Tomomi Kiyomitsu. Optogenetic reconstitution reveals that   Dynein-Dynactin-NuMA clusters generate cortical spindle-pulling forces as a   multi-arm ensemble. bioRxiv 277202; doi: https://doi.org/10.1101/277202

[10]

https://labchem.wako-chem.co.jp/journal/docs/proup10.pdf<链接>

[11]

Ince T A, Aster J C. In vitro   culture conditions for T-cell acute lymphoblastic leukemia/lymphoma: U.S.   Patent 9,683,217[P]. 2017-6-20.

[12]

Morris C D, Azadnia P, de Val N,   et al. Differential Antibody Responses to Conserved HIV-1 Neutralizing   Epitopes in the Context of Multivalent Scaffolds and Native-Like gp140   Trimers[J]. mBio, 2017, 8(1): e00036-17.<链接>

[13]

Lee K, Saetern O C, Nguyen A, et   al. Derivation of Leptomeninges Explant Cultures from Postmortem Human Brain   Donors[J]. JoVE (Journal of Visualized Experiments), 2017 (119):   e55045-e55045.<链接>

[14]

Buenrostro J D, Corces R, Wu B,   et al. Single-cell epigenomics maps the continuous regulatory landscape of   human hematopoietic differentiation[J]. bioRxiv, 2017: 109843.<链接>

[15]

Edmonds R E, Garvican E R, Smith   R K W, et al. Influence of commonly used pharmaceutical agents on equine bone   marrow‐derived mesenchymal stem cell viability[J]. Equine veterinary journal,   2017, 49(3): 352-357.<链接>

[16]

Jitraruch S, Dhawan A, Hughes R   D, et al. Cryopreservation of Hepatocyte Microbeads for Clinical   Transplantation[J]. Cell transplantation, 2017.<链接>

[17]

Usarek E, Barańczyk-Kuźma A,   Kaźmierczak B, et al. Validation of qPCR reference genes in lymphocytes from   patients with amyotrophic lateral sclerosis[J]. PloS one, 2017, 12(3):   e0174317.<链接>

[18]

Gagnon E, Connolly A, Dobbins J,   et al. Studying Dynamic Plasma Membrane Binding of TCR-CD3 Chains During   Immunological Synapse Formation Using Donor-Quenching FRET and FLIM-FRET[J].   The Immune Synapse: Methods and Protocols, 2017: 259-289.<链接>

[19]

Foster K, Chaddock J, Penn C, et   al. Non-cytotoxic protein conjugates: U.S. Patent 9,474,807[P]. 2016-10-25.

[20]

Araki N, Iida M, Machida K.   Bioassay method for detecting physiologically active substance: U.S. Patent   9,316,588[P]. 2016-4-19.

[21]

Sazinsky S, Michaelson J S,   Sathyanarayanan S, et al. Antibodies to icos: U.S. Patent Application   15/076,867[P]. 2016-3-22

[22]

李凯. Studies on Innate Immune   Activation by HBV Infection and Its Sensing Mechanism in Hepatocytes[J].   2016.

[23]

Ip L R H. Effect of INPP4B loss   on DNA repair and treatment strategies in ovarian cancer[D]. UCL (University   College London), 2016.

[24]

Thakkar A. Novel hormonal   combination therapy for triple negative breast cancer[D]. University of   Miami, 2016.

[25]

Pakdaman Y. In-vitro   characterization of STUB1 mutations in recessively inherited spinocerebellar   ataxia-16[D]. The University of Bergen, 2016.

[26]

Caxaria S. Induced pluripotent   stem cells (iPSCs) for research and therapy: induction of hepatic   differentiation in iPSCs and evaluation of their quality as a model of in   vivo development in the context of coagulation[D]. UCL (University College   London), 2016.<链接>

[27]

Bayne R A, Donnachie D J,   Kinnell H L, et al. BMP signalling in human fetal ovary somatic cells is   modulated in a gene-specific fashion by GREM1 and GREM2[J]. MHR: Basic   science of reproductive medicine, 2016, 22(9): 622-633.<链接>

[28]

Friedrich D. HIF-1 [alpha]   Drives Fungal Immunity in Human Macrophages[D]. Universität zu Lübeck,   2016.<链接>

[29]

Yasuda M, Kawabata J,   Akieda-Asai S, et al. Guanylyl cyclase C and guanylin reduce fat droplet   accumulation in cattle mesenteric adipose tissue[J]. The Journal of   Veterinary Science, 2016.<链接>

[30]

Campa M J, Moody M A, Zhang R,   et al. Interrogation of individual intratumoral B lymphocytes from lung   cancer patients for molecular target discovery[J]. Cancer Immunology,   Immunotherapy, 2016, 65(2): 171-180.<链接>

[31]

Kobayashi T, Yagi Y, Nakamura T.   Development of Genome Engineering Tools from Plant-Specific PPR Proteins   Using Animal Cultured Cells[J]. Chromosome and Genomic Engineering in Plants:   Methods and Protocols, 2016: 147-155.<链接>

[32]

Shikata H, Kaku M, Kojima S I,   et al. The effect of magnetic field during freezing and thawing of rat bone   marrow-derived mesenchymal stem cells[J]. Cryobiology, 2016, 73(1):   15-19.<链接>

[33]

Hirakawa M, Matos T, Liu H, et   al. Low-dose IL-2 selectively activates subsets of CD4+ Tregs and NK   cells[J]. JCI insight, 2016, 1(18).<链接>

[34]

Durruthy-Durruthy J, Sebastiano   V, Wossidlo M, et al. The primate-specific noncoding RNA HPAT5 regulates   pluripotency during human preimplantation development and nuclear   reprogramming[J]. Nature genetics, 2016, 48(1): 44-52.<链接>

[35]

Caxaria S, Arthold S, Nathwani A   C, et al. Generation of integration-free patient specific iPS cells using   episomal plasmids under feeder free conditions[J]. Patient-Specific Induced   Pluripotent Stem Cell Models: Generation and Characterization, 2016: 355-366.<链接>

[36]

Nonomura Y, Otsuka A, Nakashima   C, et al. Peripheral blood Th9 cells are a possible pharmacodynamic biomarker   of nivolumab treatment efficacy in metastatic melanoma patients[J].   Oncoimmunology, 2016, 5(12): e1248327.<链接>

[37]

Burridge P W, Diecke S, Matsa E,   et al. Modeling cardiovascular diseases with patient-specific human   pluripotent stem cell-derived cardiomyocytes[J]. Patient-Specific Induced   Pluripotent Stem Cell Models: Generation and Characterization, 2016: 119-130.<链接>

[38]

Mendonça M C P, Soares E S, de   Jesus M B, et al. PEGylation of Reduced Graphene Oxide Induces Toxicity in   Cells of the Blood–Brain Barrier: An in Vitro and in Vivo Study[J]. Molecular   pharmaceutics, 2016, 13(11): 3913-3924.<链接>

[39]

Eldaim A, Hashimoto O, Ohtsuki   H, et al. Expression of uncoupling protein 1 in bovine muscle cells[J].   Journal of animal science, 2016, 94(12): 5097-5104.<链接>

[40]

Zhen A, Rezek V, Youn C, et al.   Stem-cell based engineered immunity against HIV infection in the humanized   mouse model[J]. JoVE (Journal of Visualized Experiments), 2016 (113):   e54048-e54048.<链接>

[41]

Bastian N A, Bayne R A,   Hummitzsch K, et al. Regulation of fibrillins and modulators of TGFβ in fetal   bovine and human ovaries[J]. Reproduction, 2016, 152(2): 127-137.<链接>

[42]

Eto K, Takayama N, Nakamura S,   et al. Method for producing differentiated cells: U.S. Patent 9,200,254[P].   2015-12-1.

[43]

Eto K, Takayama N, Nakamura S,   et al. Novel Method for Producing Differentiated Cells: U.S. Patent   Application 14/925,508[P]. 2015-10-28.

[44]

Yamashita J, Takeda M.   Cd82-positive cardiac progenitor cells: U.S. Patent Application   15/308,147[P]. 2015-4-16.

[45]

Cai Y, Sugimoto C, Arainga M, et   al. Preferential Destruction of Interstitial Macrophages over Alveolar   Macrophages as a Cause of Pulmonary Disease in Simian Immunodeficiency   Virus–Infected Rhesus Macaques[J]. The Journal of Immunology, 2015, 195(10):   4884-4891.<链接>

[46]

Kojima S I, Kaku M, Kawata T, et   al. Cranial suture-like gap and bone regeneration after transplantation of   cryopreserved MSCs by use of a programmed freezer with magnetic field in   rats[J]. Cryobiology, 2015, 70(3): 262-268.<链接>

[47]

Egawa E Y, Kitamura N, Nakai R,   et al. A DNA hybridization system for labeling of neural stem cells with SPIO   nanoparticles for MRI monitoring post-transplantation[J]. Biomaterials, 2015,   54: 158-167.<链接>

[48]

Durruthy J D, Sebastiano V.   Derivation of GMP-Compliant Integration-Free hiPSCs Using Modified mRNAs[J].   Stem Cells and Good Manufacturing Practices: Methods, Protocols, and   Regulations, 2015: 31-42.<链接>

[49]

Sato Y, Sasaki T, Takahashi S,   et al. Development of a highly reproducible system to evaluate inhibition of   cytochrome P450 3A4 activity by natural medicines[J]. Journal of Pharmacy   & Pharmaceutical Sciences, 2015, 18(4): 316-327.<链接>

[50]

Burridge P W, Holmström A, Wu J   C. Chemically defined culture and cardiomyocyte differentiation of human   pluripotent stem cells[J]. Current protocols in human genetics, 2015: 21.3.   1-21.3. 15.<链接>

[51]

Käding N. Hypoxia Regulates Host   Cell Metabolism and Thereby Enhancing Clamydia Pneumonia Growth[D]. Zentrale   Hochschulbibliothek Lübeck, 2015.<链接>

[52]

Lu S. Calcium Dependent   Regulatory Mechanism in Wolfram Syndrome: A Dissertation[J]. 2015.

[53]

Garvican E R, Cree S, Bull L, et   al. Viability of equine mesenchymal stem cells during transport and   implantation[J]. Stem cell research & therapy, 2014, 5(4): 1.<链接>

[54]

Deng X, Terunuma H, Nieda M.   Method for producing nk cell-enriched blood preparation: U.S. Patent   Application 14/508,745[P]. 2014-10-7.

[55]

Foster K, Chaddock J, Penn C, et   al. Non-cytotoxic protein conjugates: U.S. Patent 8,778,634[P]. 2014-7-15.

[56]

Ramathal C Y, Dumuthy-Durruthy   J, Pera R A R, et al. Generation of male germ cells: U.S. Patent Application   14/904,396[P]. 2014-7-10

[57]

Ince T A. Assays, methods and   kits for analyzing sensitivity and resistance to anti-cancer drugs,   predicting a cancer patient's prognosis, and personalized treatment   strategies: U.S. Patent Application 14/894,595[P]. 2014-6-4.

[58]

Nishio M, Saeki K.   Differentiation of human pluripotent stem cells into highly functional   classical brown adipocytes[J]. Methods Enzymol, 2014, 537: 177-197.<链接>

[59]

Durruthy-Durruthy J, Briggs S F,   Awe J, et al. Rapid and efficient conversion of integration-free human   induced pluripotent stem cells to GMP-grade culture conditions[J]. PloS one,   2014, 9(4): e94231.<链接>

[60]

Koido S, Homma S, Okamoto M, et   al. Treatment with Chemotherapy and Dendritic Cells Pulsed with Multiple   Wilms' Tumor 1 (WT1)–Specific MHC Class I/II–Restricted Epitopes for   Pancreatic Cancer[J]. Clinical Cancer Research, 2014, 20(16):   4228-4239.<链接>

[61]

Patz Jr E F. Antibodies   Expressed by Intratumoral B Cells as the Basis for a Diagnostic Test for Lung   Cancer[R]. DUKE UNIV DURHAM NC, 2014.<链接>

[62]

Kaku M, Shimasue H, Ohtani J, et   al. A case of tooth autotransplantation after long-term cryopreservation   using a programmed freezer with a magnetic field[J]. The Angle Orthodontist,   2014, 85(3): 518-524.<链接>

[63]

Kaku M, Koseki H, Kojima S, et   al. Cranial bone regeneration after cranioplasty using cryopreserved   autogenous bone by a programmed freezer with a magnetic field in rats[J].   CryoLetters, 2014, 35(6): 451-461.<链接>

[64]

Koido S, Kinoshita S, Mogami T,   et al. Immunological assessment of cryotherapy in breast cancer patients[J].   Anticancer research, 2014, 34(9): 4869-4876.<链接>

[65]

Sazuka S, Katsuno T, Nakagawa T,   et al. Fibrocytes are involved in inflammation as well as fibrosis in the   pathogenesis of Crohn's disease[J]. Digestive diseases and sciences, 2014,   59(4): 760-768.<链接>

[66]

Lin S L, Lee S Y, Lin Y C, et   al. Evaluation of mechanical and histological properties of cryopreserved   human premolars under short-term preservation: A preliminary study[J].   Journal of Dental Sciences, 2014, 9(3): 244-248.<链接>

[67]

Poole E, Reeves M, Sinclair J H.   The use of primary human cells (fibroblasts, monocytes, and others) to assess   human cytomegalovirus function[J]. Human Cytomegaloviruses: Methods and   Protocols, 2014: 81-98.<链接>

[68]

Garvican E R, Dudhia J, Alves A   L, et al. Mesenchymal stem cells modulate release of matrix proteins from   tendon surfaces in vitro: a potential beneficial therapeutic effect[J].   Regenerative medicine, 2014, 9(3): 295-308.<链接>

[69]

Skinner J A, Zurawski S M,   Sugimoto C, et al. Immunologic characterization of a rhesus macaque H1N1   challenge model for candidate influenza vaccine assessment[J]. Clinical and   Vaccine Immunology, 2014: CVI. 00547-14.<链接>

[70]

Terunuma H, Deng X, Nieda M.   Method for producing nk cell-enriched blood preparation: U.S. Patent   Application 14/780,394[P]. 2013-3-27.

[71]

Cho M, Yamazaki T, Endo M, et   al. Anti-Phospholipase D4 Antibody: U.S. Patent Application 14/375,266[P].   2013-1-31.

[72]

Bhandari S. Radiological,   clinical and laboratory based studies in the pathogenesis of desmoid tumours   in familial adenomatous polyposis[J]. 2013.

[73]

Gonzàlez Juncà A. Study of   molecular mechanisms implicated in the TGF-beta oncogenic effect in   Glioma[J]. 2013.

[74]

Koseki H, Kaku M, Kawata T, et   al. Cryopreservation of osteoblasts by use of a programmed freezer with a   magnetic field[J]. CryoLetters, 2013, 34(1): 10-19.<链接>

[75]

Naito H, Yoshimura M, Mizuno T,   et al. The advantages of three‐dimensional culture in a collagen hydrogel for   stem cell differentiation[J]. Journal of Biomedical Materials Research Part   A, 2013, 101(10): 2838-2845.<链接>

[76]

Stec M, Baran J, Szatanek R, et   al. Properties of monocytes generated from haematopoietic CD34+ stem cells   from bone marrow of colon cancer patients[J]. Cancer Immunology,   Immunotherapy, 2013, 62(4): 705-713.<链接>

[77]

Müller L, Brighton L E, Carson J   L, et al. Culturing of human nasal epithelial cells at the air liquid   interface[J]. Journal of visualized experiments: JoVE, 2013 (80).<链接>

[78]

Kalaszczynska I, Ruminski S,   Platek A E, et al. Substantial differences between human and ovine   mesenchymal stem cells in response to osteogenic media: how to explain and   how to manage?[J]. BioResearch open access, 2013, 2(5): 356-363.<链接>

[79]

Tamai Y, Hasegawa A, Takamori A,   et al. Potential Contribution of a Novel Tax Epitope–Specific CD4+ T Cells to   Graft-versus-Tax Effect in Adult T Cell Leukemia Patients after Allogeneic   Hematopoietic Stem Cell Transplantation[J]. The Journal of Immunology, 2013,   190(8): 4382-4392.<链接>

[80]

Kasai K, Nakashima H, Liu F, et   al. Toxicology and biodistribution studies for MGH2. 1, an oncolytic virus   that expresses two prodrug-activating genes, in combination with prodrugs[J].   Molecular Therapy-Nucleic Acids, 2013, 2: e113.<链接>

[81]

Deng X, Terunuma H, Nieda M.   Method for producing nk cell-enriched blood preparation: U.S. Patent   Application 13/980,777[P]. 2012-1-17.

[82]

Somm E, Bonnet N, Martinez A, et   al. A botulinum toxin–derived targeted secretion inhibitor downregulates the   GH/IGF1 axis[J]. The Journal of clinical investigation, 2012, 122(9):   3295.<链接>

[83]

Takaoka E, Sonobe H, Akimaru K,   et al. Multiple sites of highly amplified DNA sequences detected by molecular   cytogenetic analysis in HS-RMS-2, a new pleomorphic rhabdomyosarcoma cell   line[J]. American journal of cancer research, 2012, 2(2): 141.<链接>

[84]

Fahlbusch F B, Dawood Y, Hartner   A, et al. Cullin 7 and Fbxw 8 expression in trophoblastic cells is regulated   via oxygen tension: implications for intrauterine growth restriction?[J]. The   Journal of Maternal-Fetal & Neonatal Medicine, 2012, 25(11): 2209-2215.<链接>

[85]

Aloé S, Weber F, Behr B, et al.   Modulatory effects of bovine seminal plasma on uterine inflammatory   processes[J]. Reproduction in domestic animals, 2012, 47(1): 12-19.<链接>

[86]

Gupta A, Bhakta S. An integrated   surrogate model for screening of drugs against Mycobacterium tuberculosis[J].   Journal of antimicrobial chemotherapy, 2012, 67(6): 1380-1391.<链接>

[87]

Saeki K. Feeder-Free Culture for   High Efficiency Production of Subculturable Vascular Endothelial Cells from   Human Embryonic Stem Cells[J]. Human Embryonic and Induced Pluripotent Stem   Cells: Lineage-Specific Differentiation Protocols, 2012: 277-294.<链接>

[88]

Yamazaki T, Okabe H, Kobayashi   S, et al. Cancer stem cell mass and process for production thereof: U.S.   Patent Application 13/878,181[P]. 2011-10-6.

[89]

Deng X, Terunuma H, Nieda M.   Method for producing nk cell-enriched blood product: U.S. Patent Application   13/577,476[P]. 2011-2-4.

[90]

Sugii S, Kida Y, Berggren W T,   et al. Feeder-independent ips cell derivation from human and mouse adipose   stem cells[J]. Nature protocols, 2011, 6(3): 346.<链接>

[91]

Shinada T, Akimoto T, Zhu Y, et   al. Modulation of viability of live cells by focused ion‐beam exposure[J].   Biotechnology and bioengineering, 2011, 108(1): 222-225.<链接>

[92]

Huang M S, Chang W J, Huang H M,   et al. Effects of transportation time after extraction on the magnetic   cryopreservation of pulp cells of rat dental pulp[J]. Journal of Dental   Sciences, 2011, 6(1): 48-52.<链接>

[93]

Sato D, Suzuki Y, Kano T, et al.   Tonsillar TLR9 expression and efficacy of tonsillectomy with steroid pulse   therapy in IgA nephropathy patients[J]. Nephrology Dialysis Transplantation,   2011, 27(3): 1090-1097.<链接>

[94]

Kamada H, Kaku M, Kawata T, et   al. In-vitro and in-vivo study of periodontal ligament cryopreserved with a   magnetic field[J]. American Journal of Orthodontics and Dentofacial   Orthopedics, 2011, 140(6): 799-805.<链接>

[95]

Bui H T, Wakayama S, Mizutani E,   et al. Essential role of paternal chromatin in the regulation of   transcriptional activity during mouse preimplantation development[J].   Reproduction, 2011, 141(1): 67-77.<链接>

[96]

Takata Y, Kishine H, Sone T, et   al. Generation of iPS cells using a BacMam multigene expression system[J].   Cell structure and function, 2011, 36(2): 209-222.<链接>

[97]

Benko Z, Zhao R Y. Zeocin for   selection of bleMX6 resistance in fission yeast[J]. Biotechniques, 2011,   51(1): 57-60.<链接>

[98]

Abedini S, Kaku M, Kawata T, et   al. Effects of cryopreservation with a newly-developed magnetic field   programmed freezer on periodontal ligament cells and pulp tissues[J].   Cryobiology, 2011, 62(3): 181-187.<链接>

[99]

Oshima-Sudo N, Li Q, Hoshino Y,   et al. Optimized method for culturing outgrowth endothelial progenitor   cells[J]. Inflammation and Regeneration, 2011, 31(2): 219-227.<链接>

[100]

Araki N. Bioassay method for   antibody against thyroid-stimulating hormone receptor, measurement kit for   the antibody, and novel genetically modified cell for use in the bioassay   method or the measurement kit: U.S. Patent Application 13/381,402[P]. 2010-6-24.

[101]

Foster K, Chaddock J, Marks P,   et al. Fusion proteins: U.S. Patent 7,659,092[P]. 2010-2-9.

[102]

Mieno S, Boodhwani M, Robich M   P, et al. Effects of diabetes mellitus on VEGF‐induced proliferation response   in bone marrow derived endothelial progenitor cells[J]. Journal of cardiac   surgery, 2010, 25(5): 618-625.<链接>

[103]

Kaku M, Kamada H, Kawata T, et   al. Cryopreservation of periodontal ligament cells with magnetic field for   tooth banking[J]. Cryobiology, 2010, 61(1): 73-78.<链接>

[104]

Kawata T, Kaku M, Fujita T, et   al. Water molecule movement by a magnetic field in freezing for tooth   banking[J]. Biomedical Research, 2010, 21(4).<链接>

[105]

Lee S Y, Chiang P C, Tsai Y H,   et al. Effects of cryopreservation of intact teeth on the isolated dental   pulp stem cells[J]. Journal of Endodontics, 2010, 36(8): 1336-1340.<链接>

[106]

Huang Y H, Yang J C, Wang C W,   et al. Dental stem cells and tooth banking for regenerative medicine[J].   Journal of Experimental & Clinical Medicine, 2010, 2(3):   111-117.<链接>

[107]

Kwon H J, Enomoto T, Shimogawara   M, et al. Benchmarks[J]. Biotechniques, 2010, 48: 460-462.<链接>

[108]

Shimizu Y, Takamori A,   Utsunomiya A, et al. Impaired Tax‐specific T‐cell responses with insufficient   control of HTLV‐1 in a subgroup of individuals at asymptomatic and smoldering   stages[J]. Cancer science, 2009, 100(3): 481-489.<链接>

[109]

Park H S, Cho S G, Park M J, et   al. Bone marrow T cells are superior to splenic T cells to induce chimeric   conversion after non-myeloablative bone marrow transplantation[J]. The Korean   journal of internal medicine, 2009, 24(3): 252.<链接>

[110]

Enosawa S, Miyamoto Y, Ikeya T.   Frozen cell immobilized product, primary hepatocyte culture tool, and method   for producing primary hepatocyte culture tool: U.S. Patent Application   12/738,809[P]. 2008-9-11.

[111]

DePinho R A, Stommel J M.   Receptor tyrosine kinase profiling: U.S. Patent Application 12/450,820[P].   2008-4-11.

[112]

Mieno S, Clements R T, Boodhwani   M, et al. Characteristics and Function of Cryopreserved Bone Marrow–Derived   Endothelial Progenitor Cells[J]. The Annals of thoracic surgery, 2008, 85(4):   1361-1366.<链接>

[113]

Warren C. The Response of HN4   Cells to Porphyromonas gingivalis DNA[D]. , 2008.

[114]

Hikichi T, Wakayama S, Mizutani   E, et al. Differentiation potential of parthenogenetic embryonic stem cells   is improved by nuclear transfer[J]. Stem Cells, 2007, 25(1): 46-53.<链接>

[115]

Zaidi S K, Pande S, Pratap J, et   al. Runx2 deficiency and defective subnuclear targeting bypass senescence to   promote immortalization and tumorigenic potential[J]. Proceedings of the   National Academy of Sciences, 2007, 104(50): 19861-19866.<链接>

[116]

Hikichi T, Wakayama S, Mizutani   E, et al. Differentiation potential of parthenogenetic embryonic stem cells   is improved by nuclear transfer[J]. Stem Cells, 2007, 25(1): 46-53.<链接>

[117]

Liu D G, Kobayashi T, Onishi A,   et al. Relation between human decay‐accelerating factor (hDAF) expression in   pig cells and inhibition of human serum anti‐pig cytotoxicity: value of   highly expressed hDAF for xenotransplantation[J]. Xenotransplantation, 2007,   14(1): 67-73.<链接>

[118]

Ishii H, Iinuma A, Osumi K, et   al. Canine tumor treatment method, pharmaceutical formulation applied   thereto, and method of cryogenically preserving cells used therewith: U.S.   Patent Application 11/465,892[P]. 2006-8-21.

[119]

Hatoya S, Sugiyama Y, Torii R,   et al. Effect of co-culturing with embryonic fibroblasts on IVM, IVF and IVC   of canine oocytes[J]. Theriogenology, 2006, 66(5): 1083-1090.<链接>

[120]

Sasaki M, Kato Y, Yamada H, et   al. Development of a novel serum‐free freezing medium for mammalian cells   using the silk protein sericin[J]. Biotechnology and applied biochemistry,   2005, 42(2): 183-188.<链接>

[121]

Haynes J E. Pseudonyms of   Authors: Including Anonyms and Initialisms[M]. JE Haynes, 1882.<链接>