Cellmatrix^® 是拥有30年以上历史的胶原蛋白研究试剂，其中TypeⅠ-A可快速凝胶化且可调整高透明度，是非常适合用于培养胶原蛋白凝胶的产品。

除此之外，通过3D细胞培养技术还可实现接近生物体内环境的条件下进行培养。

【可应用的研究领域】细胞再生医疗研究、药学/药理学研究、三维器官、生物材料等

Cellmatrix^® Type I-A

 ●　猪腱来源，酸可溶性的Type-I胶原蛋白

 ●　浓度3.0 mg/mL、pH3的无菌溶液

 ●　高凝胶强度，适合胶原蛋白・凝胶包埋培养

 ●　凝胶的透明度高，容易显微镜观察

 ※　产品有效期：自生产日期起1年

 Cellmatrix^® Type I-P

 ●　猪腱来源的胃蛋白酶可溶化的Type-I胶原蛋白

 ●　浓度3.0 mg/mL、pH3的无菌溶液

 ●　粘性低、容易处理

 ●　形成凝胶

 ※　产品有效期：自生产日期起1年

 Cellmatrix^® Type I-C

 ●　猪皮来源的胃蛋白酶可溶化的Type-I胶原蛋白

 ●　浓度3.0 mg/mL、 pH3的无菌溶液

 ●　低粘度，适合包被

 ●　几乎不会凝胶化

 ※　产品有效期：自生产日期起2年

 Cellmatrix^® Type Ⅳ

 ●　用胃蛋白酶处理牛晶状体前囊精制的Type-Ⅳ胶原蛋白

 ●　低粘度、适合包被

 ●　浓度3.0 mg/mL、pH3的无菌溶液

 ●　不会凝胶化

 ※　产品有效期：自生产日期起2年

Cellmatrix产品

凝胶性能

包被性

Type Ⅰ-A

◎

△

Type Ⅰ-P

〇

〇

Type Ⅰ-C

△

◎

Type Ⅳ

×

◎

此套装产品中包含的实验所必要的试剂，让刚开始使用Cellmatrix^®Ⅰ-A进行凝胶培养的用户也能轻松上手。

套装组成

●　Cellmatrix Type I-A ——20 mL×1

●　Ham F-12培养液——5 mL×1

●　MEM Hanks培养液——5 mL×1

●　重构缓冲液——4mL×5

本产品是为了以胶原蛋白凝胶培养为目的配制的浓缩培养液。

系列产品

Ham F-12培养液（10倍浓缩）

MEM Hanks培养液（10倍浓缩）

DF培养液（DME：F-12＝1：1）（10倍浓缩）

199培养液（10倍浓缩）

DME培养液（5倍浓缩）

RPMI-1640培养液（5倍浓缩）

保存条件：冷藏保存（4℃～8℃）

产品有效期：4个月

缓冲液组成

氢氧化钠——50 mM

碳酸氢钠——260 mM

HEPES——200 mM

保存条件：冷藏保存（4℃～8℃）

产品有效期：4个月

Cellmatrix^® 胶原蛋白用细胞培养指南

Nitta-Gelatin Product Guide

Matsunaga, M., Hatta, K., Nagafuchi, A., & Takeichi, M. (1988). Guidance of optic nerve fibres by n-cadherin adhesion molecules. Nature, 334(6177), 62-4.

【Cellmatrix^® Type I-A. IF:43.07】

Morishita, A. , Kumabe, S. , Nakatsuka, M. , & Iwai, Y. . (2014). A histological study of mineralised tissue formation around implants with 3d culture of hms0014 cells in cellmatrix type i-a collagen gel scaffold in vitro. Okajimas Folia Anatomica Japonica, 91(3), 57-71.

【Cellmatrix^® Type I-A】

Nakao, K., Itoh, M., Tomita, Y., Tomooka, Y., & Tsuji, T. (2004). Fgf-2 potently induces both proliferation and dsp expression in collagen type i gel cultures of adult incisor immature pulp cells. Biochemical & Biophysical Research Communications, 325(3), 1052-1059.

【Cellmatrix^® Type I-A. IF:2.705】

Udagawa, N., Takahashi, N., Akatsu, T., Tanaka, H., Sasaki, T., & Nishihara, T., et al. (1990). Origin of osteoclasts: mature monocytes and macrophages are capable of differentiating into osteoclasts under a suitable microenvironment prepared by bone marrow-derived stromal cells. Proceedings of the National Academy of Sciences of the United States of America, 87(18), 7260-7264.

【Cellmatrix^® Type I-A. IF:9.58】

Koide, N., Sakaguchi, K., Koide, Y., Asano, K., Kawaguchi, M., & Matsushima, H., et al. (1990). Formation of multicellular spheroids composed of adult rat hepatocytes in dishes with positively charged surfaces and under other nonadherent environments. Experimental Cell Research, 186(2), 0-235.

【Cellmatrix^® Type I-A ，Cellmatrix® Type III，Cellmatrix® Type IV. IF:3.329】

Jabaji, Z., Brinkley, G. J., Khalil, H. A., Sears, C. M., Nan, Y. L., & Lewis, M., et al. (2014). Type i collagen as an extracellular matrix for the in vitro growth of human small intestinal epithelium. Plos One, 9(9), e107814.

【Cellmatrix^® Type I-A. IF:2.776】

Shakado, S., Sakisaka, S., Noguchi, K., Yoshitake, M., Harada, M., & Mimura, Y., et al. (2010). Effects of extracellular matrices on tube formation of cultured rat hepatic sinusoidal endothelial cells. Hepatology, 22(3), 969-973.

【Cellmatrix^® Type I-A，Cellmatrix^® Type IV. IF:14.971】

Tamura, T., Udagawa, N., Takahashi, N., Miyaura, C., Tanaka, S., & Yamada, Y., et al. (1993). Soluble interleukin-6 receptor triggers osteoclast formation by interleukin 6. Proceedings of the National Academy of Sciences of the United States of America, 90(24), 11924-11928.

【Cellmatrix^® Type I-A. IF:9.58】

Takahashi, N., Udagawa, N., Akatsu, T., Tanaka, H., Isogai, Y., & Suda, T. (1991). Deficiency of osteoclasts in osteopetrotic mice is due to a defect in the local microenvironment provided by osteoblastic cells*. Endocrinology, 128(4), 1792-1796.

【Cellmatrix^® Type I-A. IF:3.8】

Mano, H. . (1996). Mammalian mature-osteoclasts as estrogen target cells. Biochemical & Biophysical Research Communications, 223(3), 637-642.

【Cellmatrix^® Type I-A. IF:2.705】

Udagawa, N., Takahashi, N., Jimi, E., Matsuzaki, K., Tsurukai, T., & Itoh, K., et al. (1999). Osteoblasts/stromal cells stimulate osteoclast activation through expression of osteoclast differentiation factor/rankl but not macrophage colony-stimulating factor: receptor activator of nf-kappa b ligand. Bone, 25(5), 517-523.

【Cellmatrix^® Type I-A. IF:4.36】

12．

Woo, J. T., Kasai, S., Stern, P. H., & Nagai, K. (2010). Compactin suppresses bone resorption by inhibiting the fusion of prefusion osteoclasts and disrupting the actin ring in osteoclasts. Journal of Bone & Mineral Research, 15(4), 650-662.

【Cellmatrix^® Type I-A. IF:5.711】

Ishida, K., Murofushi, M., Nakao, K., Morita, R., Ogawa, M., & Tsuji, T. (2011). The regulation of tooth morphogenesis is associated with epithelial cell proliferation and the expression of sonic hedgehog through epithelial–mesenchymal interactions. Biochemical & Biophysical Research Communications, 405(3), 455-461.

【Cellmatrix^® Type I-A. IF:2.705】

Nakagawa, H. , Wachi, M. , Woo, J. T. , Kato, M. , Kasai, S. , & Takahashi, F. , et al. (2002). Fenton reaction is primarily involved in a mechanism of (?)-epigallocatechin-3-gallate to induce osteoclastic cell death. Biochemical and Biophysical Research Communications, 292(1), 94-101.

【Cellmatrix^® Type I-A. IF:2.705】

Onodera, K. I., Fukatsu, T., Kawai, N., Yoshioka, Y., Okamoto, T., & Nakamura, H., et al. (2004). Zooxanthellactone, a novel γ-lactone-type oxylipine from dinoflagellates of symbiodinium sp.: structure, distribution, and biological activity. Bioscience Biotechnology & Biochemistry, 68(4), 848-852.

【Cellmatrix^® Type I-A. IF:1.297】

Torisawa, Y. S., Shiku, H., Yasukawa, T., Nishizawa, M., & Matsue, T. (2005). Multi-channel 3-d cell culture device integrated on a silicon chip for anticancer drug sensitivity test. Biomaterials, 26(14), 2165-2172.

【Cellmatrix^® Type I-A. IF:10.273】

Murakami, H., Takahashi, N., Tanaka, S., Nakamura, I., Udagawa, N., & Nakajo, S., et al. (1997). Tiludronate inhibits protein tyrosine phosphatase activity in osteoclasts. Bone, 20(5), 399-404.

【Cellmatrix^® Type I-A. IF:4.36】

Fujimoto, M., & Mihara, S. I. (1991). Two states of the l-type ca 2+ channel in pc12 cells: different sensitivity to 1,4-dihydropyridines. Neuroscience Letters, 122(1), 9-12.

【Cellmatrix^® Type I-A. IF:2.173】

Kawamura, S., Wakitani, S., Kimura, T., Maeda, A., Caplan, A. I., & Shino, K., et al. (1998). Articular cartilage repair: rabbit experiments with a collagen gel-biomatrix and chondrocytes cultured in it. Acta Orthop Scand, 69(1), 56-62.

【Cellmatrix^® Type I-A】

Tilney, L. G., Derosier, D. J., & Mulroy, M. J. (1980). The organization of actin filaments in the stereocilia of cochlear hair cells. Journal of Cell Biology, 86(1), 244-259.

【Cellmatrix^® Type III，Cellmatrix^® Type IV. IF:8.891】

Yoshii, C., Ueda, Y., Okamoto, M., & Araki, M. (2007). Neural retinal regeneration in the anuran amphibian xenopus laevis post-metamorphosis: transdifferentiation of retinal pigmented epithelium regenerates the neural retina. Developmental Biology, 303(1), 45-56.

【Cellmatrix^® Type I-C. IF:2.936】

Aoki, K. , & Matsuda, M. . (2009). Visualization of small gtpase activity with fluorescence resonance energy transfer-based biosensors. NATURE PROTOCOLS, 4(11), 1623-1631.

【Cellmatrix^® Type I-C. IF:11.334】

Minakawa, M., Miura, Y., & Yagasaki, K. (2012). Piceatannol, a resveratrol derivative, promotes glucose uptake through glucose transporter 4 translocation to plasma membrane in l6 myocytes and suppresses blood glucose levels in type 2 diabetic model db/db mice. Biochemical & Biophysical Research Communications, 422(3), 469-475.

【Cellmatrix^® Type I-C. IF:2.705】

Koide, T., Homma, D. L., Asada, S., & Kitagawa, K. (2005). Self-complementary peptides for the formation of collagen-like triple helical supramolecules. Bioorganic & Medicinal Chemistry Letters, 15(23), 5230-5233.

【Cellmatrix^® Type I-C. IF:2.448】

Izuta, H., Shimazawa, M., Tsuruma, K., Araki, Y., Mishima, S., & Hara, H. (2009). Bee products prevent vegf-induced angiogenesis in human umbilical vein endothelial cells. Bmc Complementary & Alternative Medicine, 9(1), 45.

【Cellmatrix^® Type I-C. IF:2.479】

Umigai, & Naofumi. (2012). Crocetin, a carotenoid derivative, inhibits vegf-induced angiogenesis via suppression of p38 phosphorylation. Current Neurovascular Research, 9(2), 102-109.

【Cellmatrix^® Type I-C. IF:1.811】

Isaji, M., Miyata, H., Ajisawa, Y., Takehana, Y., & Yoshimura, N. (2010). Tranilast inhibits the proliferation, chemotaxis and tube formation of human microvascular endothelial cells in vitro and angiogenesis in vivo. British Journal of Pharmacology, 122(6), 1061-1066.

【Cellmatrix^® Type I-P. IF:6.583】

Tokunaga, T., Kiso, T., Namikawa, T., & Ohtsubo, Y. (1999). Camp-independent chloride secretion activated by a vasoactive intestinal peptide in a monolayer culture of human bronchial epithelial cells. Biological & Pharmaceutical Bulletin, 22(7), 745-748.

【Cellmatrix^® Type I-C. IF:1.54】

Aoki, Y. , Satoh, K. , Sato, K. , & Suzuki, K. T. . (1992). Induction of glutathioner, sr, -transferase p-form in primary cultured rat liver parenchymal cells by co-planar polychlorinated biphenyl congeners. Biochemical Journal, 281(2), 539-543.

【Cellmatrix® Type I-P. IF:4.331】

Yamamoto, M., Kato, K., & Ikada, Y. (2015). Ultrastructure of the interface between cultured osteoblasts and surface-modified polymer substrates. Journal of Biomedical Materials Research, 37(1), 29-36.

【Cellmatrix^® Type I-P】

Ishihara, S., Haga, H. M., Mizutani, T., Kawabata, K., Shirato, H., & Nishioka, T. (2010). Integrin beta1-dependent invasive migration of irradiation-tolerant human lung adenocarcinoma cells in 3d collagen matrix. Biochemical & Biophysical Research Communications, 396(3), 651-655.

【Cellmatrix^® Type I-C，Cellmatrix^® Type I-P. IF:2.705】

Suzuki, M., Ichikawa, K., Sakoda, A., & Sakai, Y. (1993). Long-term culture of primary rat hepatocytes with high albumin secretion using membrane-supported collagen sandwich. Cytotechnology, 11(3), 213.

【Cellmatrix^® Type I-P. IF:1.672】

Nakaoka, R. , Tsuchiya, T. , Kato, K. , Ikada, Y. , & Nakamura, A. . (1997). Studies on tumor-promoting activity of polyethylene: inhibitory activity of metabolic cooperation on polyethylene surfaces is markedly decreased by surface modification with collagen but not with rgds peptide. Journal of biomedical materials research, 35(3), 391-397.

【Cellmatrix^® Type I-P】