上海金畔生物科技有限公司代理UELANDY荧光染料全系系列产品
DAPI染色液（即用型）
产品货号: D4080
产品规格: 10 mL
目录价（元）:100
推荐仪器：荧光显微镜
大包装询价
产品概述：
产品参数
Ex/Em（结合 DNA）=360/460 nm
CAS 号：28718-90-3
分子量：350.25
分子式：C16H17Cl2N5
储存条件
-20℃ 避光保存，有效期见外包装。
产品介绍
DAPI 是一种可对 DNA 染色的细胞核染色试剂，它在嵌入双链 DNA 后释放蓝色荧光，亮度增强约 20 倍。DAPI 常用于细胞凋亡检测，染色后用荧光显微镜观察或流式细胞仪检测。DAPI 具有很高的光漂白承受水平，能用来检测酵母线粒体 DNA、叶绿体 DNA、病毒 DNA 以及染色 DNA 等。
注意事项
1. 本染色液的浓度经过优化的，可以满足各种常规染色的需要。如需调整使用浓度，请选择 D4054，自行配置合适的工作液浓度；
2. DAPI 对人体有害，请注意适当防护；
3. 荧光染料均存在淬灭问题，请尽量注意避光，以减缓淬灭。
说明书：
UE-D4080
使用本产品的文献：
1.Extracellular matrix stiffness controls osteogenic differentiation of mesenchymal stem cells mediated by integrin α5
Meiyu Sun, Guangfan Chi, Juanjuan Xu, Ye Tan, Jiayi Xu, Shuang Lv, Ziran Xu, Yuhan Xia, Lisha Li and Yulin Li
Stem Cell Research &Therapy (2018)
应用方向：复染
2.Transcriptomic and Quantitative Proteomic Analyses Provide Insights Into the Phagocytic Killing of Hemocytes in the Oyster Crassostrea gigas
Shuai Jiang, Limei Qiu, Lingling Wang, Zhihao Jia, Zhao Lv, Mengqiang Wang,Conghui Liu, Jiachao Xu and Linsheng Song
Frontiers in Immunology (2018)
应用方向：复染
3.Overcoming the Reticuloendothelial System Barrier to Drug Delivery with a “Don’t-Eat-Us” Strategy
Yixuan Tang，Xiaoyou Wang，Jie Li，Yu Nie，Guojian Liao，Yang Yu，Chong Li
ACS NANO
应用方向：复染
参考文献
1.Assay for nanogram quantities of DNA in cellular homogenates
应用方向：DNA含量测定
2.A simple cytochemical techniqUE for demonstration of DNA in cells infected with mycoplasmas and viruses
应用方向：诊断细胞培养的支原体感染
3.Stage-specific differences in cell cycle control in Trypanosoma brucei revealed by RNA interference of a mitotic cyclin
应用方向：流式细胞术中细胞核含量测定及染色体分选
4.Loss of HSulf-1 up-regulates heparin-binding growth factor signaling in cancer
应用方向：评估细胞凋亡
5.Direct measurement of femtogram amounts of DNA in cells and chloroplasts by quantitative microspectrofluorometry.
应用方向：免疫荧光法和原位杂交法检测细胞核和细胞器DNA
6.SMAD2 and SMAD7 Involvement in the post-translational regulation of Muc4 via the transforming growth factor-β and interferon-γ pathways in rat mammary epithelial cells
应用方向：免疫荧光法和原位杂交法检测细胞核和细胞器DNA
7.A simple cytochemical techniqUE for demonstration of DNA in cells infected with mycoplasmas and viruses
应用方向：取代溴化乙锭对琼脂糖凝胶中的DNA进行染色
8.Comparison of ethidium bromide and 4′, 6′-diamidino-2-phenylindole as quantitative fluorescent stains for DNA in agarose gels
应用方向：取代溴化乙锭对琼脂糖凝胶中的DNA进行染色
9.Localization of polyphosphates in Saccharomyces fragilis, as revealed by 4´, 6-diamidino-2-phenylindole fluorescence
应用方向：DAPI可与多磷酸盐和其他聚阴离子，硫酸葡聚糖和SDS结合
10.InflUEnce of S-adenosylmethionine on 4´, 6-diamidino-2-phenylindole dihydrochloride (DAPI)-induced fluorescence of polyphosphate in the yeast vacuole
应用方向：DAPI可与多磷酸盐和其他聚阴离子，硫酸葡聚糖和SDS结合
11.Fluorescent Complexes of DNA With DAPI 4′,6-diamidine-2-phenyl indole.2HCl or DCI 4′,6-dicarboxyamide-2-phenyl Indole
应用方向：DAPI可与多磷酸盐和其他聚阴离子，硫酸葡聚糖和SDS结合