Ginkgetin(Synonyms: 银杏双黄酮)

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天然产物 黄酮类 Flavonoids

Ginkgetin (Synonyms: 银杏双黄酮) 纯度: 98.37%

Ginkgetin 是一种从银杏叶中分离得到的双黄酮。Ginkgetin 具有抗肿瘤，抗炎，神经保护，抗真菌的作用。Ginkgetin 也是一种有效的 Wnt 信号抑制剂，IC50 值为 5.92 μM。

Ginkgetin(Synonyms: 银杏双黄酮)

Ginkgetin Chemical Structure

CAS No. : 481-46-9

规格 价格 是否有货 数量
10 mM * 1 mL in DMSO ￥3116 In-stock
5 mg ￥2500 In-stock
10 mg ￥4200 In-stock
50 mg   询价  
100 mg   询价  

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生物活性

Ginkgetin, a biflavone, is isolated from Ginkgo biloba leaves. Ginkgetin exhibit anti-tumor, anti-inflammatory, neuroprotective, anti-fungal activities. Ginkgetin is also a potent inhibitor of Wnt signaling, with an IC50 of 5.92 μΜ[1][2][3][4][5].

IC50 & Target[2]

Wnt

5.92 μM (IC50)

体外研究
(In Vitro)

Ginkgetin (2.5-20 μM; 48 h) inhibits the growth of Daoy and D283 cell lines, and induces G2/M cell cycle arrest in Daoy cells[2].
Ginkgetin (20-40 μM; 24 h) significantly activates the apoptosis of osteosarcoma cells in a concentration-dependent manner[3].
Ginkgetin (10-20 μM; 3-24 h) down-regulated the expression of Wnt target genes without affecting the expression of β-catenin in medulloblastoma cells[2].
Ginkgetin (1-10 μM; 24 or 48 h) significantly inhibits the VEGF-induced endothelial cell proliferation, migration, and wound recovery in a concentration-dependent manner[1].
Ginkgetin (5-10 μM; 48 h) induces autophagy responsible for cell death in A549[5].

Shanghai Jinpan Biotech Co Ltd has not independently confirmed the accuracy of these methods. They are for reference only.

Cell Viability Assay[2]

Cell Line: Daoy and D283 cell lines
Concentration: 2.5, 5, 10, 20 μM
Incubation Time: 48 hours
Result: Inhibited the cell growth, with IC50s of 14.65 and 15.81 μM for Daoy and D283 cells, respectively.

Apoptosis Analysis[3]

Cell Line: Osteosarcoma cells
Concentration: 20, 30, 40 μM
Incubation Time: 24 hours
Result: Markedly induced the apoptosis of osteosarcoma cells in a concentration-dependent manner.

Cell Cycle Analysis[2]

Cell Line: Daoy cells
Concentration: 2.5, 5, 10, 20 μM
Incubation Time: 24 hours
Result: Increased G2/M phase, compared with that of control, indicating a G2/M cell phase arrest.

Cell Cycle Analysis[2]

Cell Line: Daoy and D283 cell lines
Concentration: 10, 20 μM
Incubation Time: 3, 6, 12, 24 hours
Result: Attenuated the expression of Wnt target genes, Axin2, cyclin D1 and survivin at 20 μM for 24 h in Daoy cells.
Unaffected the level of total β-catenin and diminished the level of β-catenin phosphorylation in a time- and concentration-dependent manner.

体内研究
(In Vivo)

Ginkgetin (25-100 mg/kg; i.p. 2 hours after the onset of ischemia) exerts anti-inflammatory effects on cerebral ischemia/reperfusion-induced injury in a rat model via the TLR4/NF-κB signaling pathway[4].
Ginkgetin (30 mg/kg; intragastrically once per day for 42 d) suppresses tumor growth in A549 cells bearing nude mice[5].

Shanghai Jinpan Biotech Co Ltd has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model: Male Sprague-Dawley rats (200-220 g)[4]
Dosage: 25, 50, 100 mg/kg
Administration: I.p. 2 hours after the onset of ischemia
Result: Reduced the neurological deficit score.
Suppressed the expression of NF-κB, TLR4 and IκBαin ischemic penumbra cortex, and inhibited the degradation of IκBα.
Decreased the expressions of ICAM-1, COX-2, and iNOS.
Downregulated downstream inflammatory factor PGE2 and TNF-α expression.
Decreased IL-1β, IL-6, IL-8, and IL-10 protein expression.

分子量

566.51

Formula

C32H22O10
CAS 号

481-46-9
中文名称

银杏素；白果双黄酮；银杏双黄酮；银杏黄素
运输条件

Room temperature in continental US; may vary elsewhere.
储存方式
Powder -20°C 3 years
4°C 2 years
In solvent -80°C 6 months
-20°C 1 month
溶解性数据
In Vitro: 

DMSO : 125 mg/mL (220.65 mM; Need ultrasonic)

配制储备液
浓度 溶剂体积 质量 1 mg 5 mg 10 mg
1 mM 1.7652 mL 8.8260 mL 17.6519 mL
5 mM 0.3530 mL 1.7652 mL 3.5304 mL
10 mM 0.1765 mL 0.8826 mL 1.7652 mL

*

请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液；一旦配成溶液，请分装保存，避免反复冻融造成的产品失效。
储备液的保存方式和期限：-80°C, 6 months; -20°C, 1 month。-80°C 储存时，请在 6 个月内使用，-20°C 储存时，请在 1 个月内使用。
参考文献

  • [1]. Hu WH, et, al. Synergy of Ginkgetin and Resveratrol in Suppressing VEGF-Induced Angiogenesis: A Therapy in Treating Colorectal Cancer. Cancers (Basel). 2019 Nov 20;11(12):1828.

    [2]. Ye ZN, et, al. Biflavone Ginkgetin, a Novel Wnt Inhibitor, Suppresses the Growth of Medulloblastoma. Nat Prod Bioprospect. 2015 Mar 29;5(2):91-97.

    [3]. Xiong M, et, al. Ginkgetin exerts growth inhibitory and apoptotic effects on osteosarcoma cells through inhibition of STAT3 and activation of caspase-3/9. Oncol Rep. 2016 Feb;35(2):1034-40.

    [4]. Li Q, et, al. Ginkgetin exerts anti-inflammatory effects on cerebral ischemia/reperfusion-induced injury in a rat model via the TLR4/NF-κB signaling pathway. Biosci Biotechnol Biochem. 2019 Apr;83(4):675-683.

    [5]. Lou J, et, al. Ginkgetin induces autophagic cell death through p62/SQSTM1-mediated autolysosome formation and redox setting in non-small cell lung cancer. Oncotarget. 2017 Oct 16;8(54):93131-93148.