Polyethylene glycol 12-hydroxystearate | Cytiva思拓凡-Whatman滤纸滤膜滤器

生化分析试剂 Biochemical Assay Reagents
Polyethylene glycol 12-hydroxystearate;

Polyethylene glycol 12-hydroxystearate, 一种 Macrogol 15 羟基硬脂酸酯，是一种通透性增强剂。

Polyethylene glycol 12-hydroxystearate Chemical Structure

CAS No. : 61909-81-7

规格	价格	是否有货	数量
10 g	￥500	In-stock
50 g	￥800	In-stock
> 100 g	;	询价	;

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生物活性	Polyethylene glycol 12-hydroxystearate, a Macrogol 15 hydroxy stearate, is a permeability enhancer^[1].
体外研究 (In Vitro)	Polyethylene glycol 12-hydroxystearate is used to increase the aqueous solubility of Biopharmaceutical Classification System (BCS) Class 2 and 4 lipophilic molecules. Polyethylene glycol 12-hydroxystearate has shown an enhancement in permeability of medium sized biological drugs across epithelia^[1]. MCE has not independently confirmed the accuracy of these methods. They are for reference only.
Formula	C₂₀H₄₀O₄
CAS 号	61909-81-7
运输条件	Room temperature in continental US; may vary elsewhere.
储存方式	4deg;C, sealed storage, away from moisture
溶解性数据	In Vitro:; Ethanol : 100 mg/mL (Need ultrasonic)
参考文献	[1]. Shubber S, et al. Mechanism of mucosal permeability enhancement of CriticalSorb (Solutol HS15) investigated in vitro in cell cultures. Pharm Res. 2015 Feb;32(2):516-27.

Cell Assay ^[1]	For both MTS and LDH cell viability assays, cells are seeded at a density of 1×10⁴ cells on 96-well plates and cultured for a minimum of 24 h. Solutol HS-15 (in 25 mM HBSS:HEPES, pH 7.4) solutions are applied at concentrations above and below the critical micelle concentration (CMC) (0.01 mM to 20 mM) and cytotoxicity is determined using the MTS and LDH assays. 25 mM HBSS:HEPES is used as a negative control and Triton-X 100 (3 mM) as a positive control. Following 3 h incubation, the MTS reagent in media and LDH working solution are added to the cells and the cells are incubated for a further 3 h at 37°C^[1]. MCE has not independently confirmed the accuracy of these methods. They are for reference only.
参考文献	[1]. Shubber S, et al. Mechanism of mucosal permeability enhancement of CriticalSorb (Solutol HS15) investigated in vitro in cell cultures. Pharm Res. 2015 Feb;32(2):516-27.

Cell Assay
^[1]

For both MTS and LDH cell viability assays, cells are seeded at a density of 1×10⁴ cells on 96-well plates and cultured for a minimum of 24 h. Solutol HS-15 (in 25 mM HBSS:HEPES, pH 7.4) solutions are applied at concentrations above and below the critical micelle concentration (CMC) (0.01 mM to 20 mM) and cytotoxicity is determined using the MTS and LDH assays. 25 mM HBSS:HEPES is used as a negative control and Triton-X 100 (3 mM) as a positive control. Following 3 h incubation, the MTS reagent in media and LDH working solution are added to the cells and the cells are incubated for a further 3 h at 37°C^[1].

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

参考文献

[1]. Shubber S, et al. Mechanism of mucosal permeability enhancement of CriticalSorb (Solutol HS15) investigated in vitro in cell cultures. Pharm Res. 2015 Feb;32(2):516-27.