Methoxy-PMS(Synonyms: 1-Methoxy PMS; 1-Methoxyphenazine methosulfate)

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生化分析试剂 Biochemical Assay Reagents
Methoxy-PMS;(Synonyms: 1-Methoxy PMS; 1-Methoxyphenazine methosulfate) 纯度: 98.34%

Methoxy-PMS (1-Methoxy PMS) 是一个稳定的电子传递中介。是活性氧生成的诱导剂。

Methoxy-PMS(Synonyms: 1-Methoxy PMS; 1-Methoxyphenazine methosulfate)

Methoxy-PMS Chemical Structure

CAS No. : 65162-13-2

规格 价格 是否有货 数量
Free Sample (0.1-0.5 mg) ; Apply now ;
10;mM;*;1 mL in DMSO ￥770 In-stock
50 mg ￥700 In-stock
100 mg ￥1100 In-stock
200 mg ￥1500 In-stock
500 mg ￥3200 In-stock
1 g ; 询价 ;
5 g ; 询价 ;

* Please select Quantity before adding items.

Methoxy-PMS 相关产品

bull;相关化合物库:

  • Bioactive Compound Library Plus
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生物活性

Methoxy-PMS (1-Methoxy PMS), an active oxygen formation inducer, is stable electron-transport mediator between NAD(P)H and tetrazolium dyes[1][2].

体外研究
(In Vitro)

Methoxy-PMS has no cytotoxicity in the cell culture media. Methoxy-PMS receives an electron from NADH or NADPH at the membrane or inside of the cell and passes the electron to the WST-8 that is around the outer cell membrane[2].

MCE has not independently confirmed the accuracy of these methods. They are for reference only.
分子量

336.36

Formula

C15H16N2O5S
CAS 号

65162-13-2
运输条件

Room temperature in continental US; may vary elsewhere.
储存方式

4deg;C, sealed storage, away from moisture and light

*In solvent : -80deg;C, 6 months; -20deg;C, 1 month (sealed storage, away from moisture and light)
溶解性数据
In Vitro:;

DMSO : 30 mg/mL (89.19 mM; Need ultrasonic and warming)

H2O : 6.67 mg/mL (19.83 mM; Need ultrasonic)

配制储备液
浓度 溶剂体积 质量 1 mg 5 mg 10 mg
1 mM 2.9730 mL 14.8650 mL 29.7301 mL
5 mM 0.5946 mL 2.9730 mL 5.9460 mL
10 mM 0.2973 mL 1.4865 mL 2.9730 mL

*

请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液；一旦配成溶液，请分装保存，避免反复冻融造成的产品失效。
储备液的保存方式和期限：-80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light)。-80°C 储存时，请在 6 个月内使用，-20°C 储存时，请在 1 个月内使用。

In Vivo:

请根据您的实验动物和给药方式选择适当的溶解方案。以下溶解方案都请先按照 In Vitro 方式配制澄清的储备液，再依次添加助溶剂：

——为保证实验结果的可靠性，澄清的储备液可以根据储存条件，适当保存；体内实验的工作液，建议您现用现配，当天使用； 以下溶剂前显示的百
分比是指该溶剂在您配制终溶液中的体积占比；如在配制过程中出现沉淀、析出现象，可以通过加热和/或超声的方式助溶

  • 1.

    请依序添加每种溶剂：;10% DMSO ;; 40% PEG300 ;; 5% Tween-80 ;; 45% saline

    Solubility: ≥ 2.25 mg/mL (6.69 mM); Clear solution

    此方案可获得 ≥ 2.25 mg/mL (6.69 mM，饱和度未知) 的澄清溶液。

    以 1 mL 工作液为例，取 100 μL 22.5 mg/mL 的澄清 DMSO 储备液加到 400 μL PEG300 中，混合均匀；向上述体系中加入50 μL Tween-80，混合均匀；然后继续加入 450 μL生理盐水定容至 1 mL。

    将 0.9 g 氯化钠，完全溶解于 100 mL ddH₂O 中，得到澄清透明的生理盐水溶液

  • 2.

    请依序添加每种溶剂：;10% DMSO ;; 90% (20% SBE-β-CD in saline)

    Solubility: ≥ 2.25 mg/mL (6.69 mM); Clear solution

    此方案可获得 ≥ 2.25 mg/mL (6.69 mM，饱和度未知) 的澄清溶液。

    以 1 mL 工作液为例，取 100 μL 22.5 mg/mL 的澄清 DMSO 储备液加到 900 μL 20% 的 SBE-β-CD 生理盐水水溶液中，混合均匀。

    将 2 g 磺丁基醚 β-环糊精加入 5 mL 生理盐水中，再用生理盐水定容至 10 mL，完全溶解，澄清透明
*以上所有助溶剂都可在 MCE 网站选购。
参考文献

  • [1]. De Niz M et al. Tools for mass screening of G6PD deficiency: validation of the WST8/1-methoxy-PMS enzymatic assay in Uganda. Malar J. 2013 Jun 19;12:210.

    [2]. Arakawa H et al. Chemiluminescence assay for tetrahydrobiopterin based on the generation of hydrogen peroxide using isoluminol-microperoxidase in the presence of 1-methoxy PMS. Luminescence. 2007 May-Jun;22(3):245-50.