荧光染料PL553 | Cytiva思拓凡-Whatman滤纸滤膜滤器

荧光染料Dye Reagents PL553;

PL553 是一种特异性的，具有高度亲和性的白三烯 A4 水解酶 (Leukotriene A4 hydrolase) 荧光发生底物，最大吸收光和发射光分别为 210 nm 和 410 nm。

PL553 Chemical Structure

CAS No. : 1456872-74-4

规格	价格	是否有货
5 mg	￥3500	询问价格货期
10 mg	￥6000	询问价格货期
25 mg	￥12000	询问价格货期

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生物活性	PL553 is a specific and high-affinity fluorigenic substrate of Leukotriene A4 hydrolase, with a λ_max of 210 nm and λ_em of 410 nm.
体外研究 (In Vitro)	PL553 is a specific and high-affinity fluorigenic substrate of Leukotriene A4 hydrolase (LTA4H), with a maximum absorption (λ_max) of 210 nm and maximum emission (λ_em) of 410 nm. PL553 is a better LTA4H substrate than (l)-Ala-β-naphthylamide, and resistant to cleavage by other aminopeptidases, but can be cleaved by FAAH. PL553 (40 μM) is used to evaluate the potencies of known inhibitors toward LTA4H^[1]. MCE has not independently confirmed the accuracy of these methods. They are for reference only.
分子量	508.49
Formula	C₂₈H₂₃F₃N₂O₄
CAS 号	1456872-74-4
运输条件	Room temperature in continental US; may vary elsewhere.
储存方式	Please store the product under the recommended conditions in the Certificate of Analysis.
参考文献	[1]. Poras H, et al. A sensitive fluorigenic substrate for selective in vitro and in vivo assay of leukotriene A4 hydrolase activity. Anal Biochem. 2013 Oct 15;441(2):152-61.

Kinase Assay ^[1]	The ability of PL553 to discriminate LTA4H from APN enzymatic activity is tested using the (l)-Ala-β-naphthylamide and PL553 peptide substrates at 40 μM with either APN from porcine kidney (0.33 mU/mL) or recombinant human LTA4H (0.6 μg/mL). The enzymatic reactions proceeds for 1 h at 37°C in a final volume of 100 μL of 50 mM Tris-HCl (pH 7.4) or 50 mM Tris-HCl (pH 7.4) and 100 mM NaCl for APN or LTA4H, respectively^[1]. MCE has not independently confirmed the accuracy of these methods. They are for reference only.
参考文献	[1]. Poras H, et al. A sensitive fluorigenic substrate for selective in vitro and in vivo assay of leukotriene A4 hydrolase activity. Anal Biochem. 2013 Oct 15;441(2):152-61.

Kinase Assay
^[1]

The ability of PL553 to discriminate LTA4H from APN enzymatic activity is tested using the (l)-Ala-β-naphthylamide and PL553 peptide substrates at 40 μM with either APN from porcine kidney (0.33 mU/mL) or recombinant human LTA4H (0.6 μg/mL). The enzymatic reactions proceeds for 1 h at 37°C in a final volume of 100 μL of 50 mM Tris-HCl (pH 7.4) or 50 mM Tris-HCl (pH 7.4) and 100 mM NaCl for APN or LTA4H, respectively^[1].

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

参考文献

[1]. Poras H, et al. A sensitive fluorigenic substrate for selective in vitro and in vivo assay of leukotriene A4 hydrolase activity. Anal Biochem. 2013 Oct 15;441(2):152-61.