荧光染料Rhodamine 800(Synonyms: 罗丹明800)

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荧光染料Dye Reagents Rhodamine 800;(Synonyms: 罗丹明800) 纯度: ge;98.0%

Rhodamine 800 是一种近红外荧光染料。

Rhodamine 800(Synonyms: 罗丹明800)

Rhodamine 800 Chemical Structure

CAS No. : 137993-41-0

规格 价格 是否有货 数量
50 mg ￥1100 In-stock
100 mg ; 询价 ;
200 mg ; 询价 ;

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Rhodamine 800 相关产品

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  • Bioactive Compound Library Plus

生物活性

Rhodamine 800 is a near-infrared fluorescent dye.

体外研究
(In Vitro)

Rhodamine 800 has different optical absorption spectra in various organic solvents and in aqueous solution in the visible and near-infrared regions. The absorption maximum at 683 nm in acetone shifts to 696 nm in DMSO without a significant change of the overall spectral profile. The fluorescence intensity of Rhodamine 800 varies markedly with the solvent, and decreases to less than one-fifth when the solvent is changed from acetone to water without a significant change of the emission peak[1].

MCE has not independently confirmed the accuracy of these methods. They are for reference only.
分子量

495.95

Formula

C26H26ClN3O5
CAS 号

137993-41-0
中文名称

罗丹明800
运输条件

Room temperature in continental US; may vary elsewhere.
储存方式

4deg;C, sealed storage, away from moisture

*In solvent : -80deg;C, 6 months; -20deg;C, 1 month (sealed storage, away from moisture)
溶解性数据
In Vitro:;

DMSO : 83.33 mg/mL (168.02 mM; Need ultrasonic)

配制储备液
浓度 溶剂体积 质量 1 mg 5 mg 10 mg
1 mM 2.0163 mL 10.0817 mL 20.1633 mL
5 mM 0.4033 mL 2.0163 mL 4.0327 mL
10 mM 0.2016 mL 1.0082 mL 2.0163 mL

*

请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液；一旦配成溶液，请分装保存，避免反复冻融造成的产品失效。
储备液的保存方式和期限：-80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)。-80°C 储存时，请在 6 个月内使用，-20°C 储存时，请在 1 个月内使用。
参考文献

  • [1]. Sakanoue J, et al. Rhodamine 800 as a probe of energization of cells and tissues in the near-infrared region: a study with isolated rat liver mitochondria and hepatocytes. J Biochem. 1997 Jan;121(1):29-37.

    [2]. Jin T. et al. Near-infrared fluorescence detection of acetylcholine in aqueous solution using a complex ofrhodamine 800 and p-sulfonatocalix[8]arene. Sensors (Basel). 2010;10(3):2438-49.
Kinase Assay
[2]

Rhodamine 800 (Rh800) and indocyanine green (ICG) are dissolved in PBS and their concentrations are set to 40 nM. To 3 mL of Rhodamine 800 solution (40 nM/PBS), 100 μL of p-sulfonatocalix[n]arenes (S[n]) (1 mM) is added. To this solution, microliter aliquots of acetylcholine (ACh) (350 mM) are added under stirring. After 2 min stirring, fluorescence spectra are measured. For the competitive fluorophore displacement by other neurotransmitters, amino acids, ammonium chloride, and choline, similar procedure as the above method is applied[2].

MCE has not independently confirmed the accuracy of these methods. They are for reference only.
参考文献

  • [1]. Sakanoue J, et al. Rhodamine 800 as a probe of energization of cells and tissues in the near-infrared region: a study with isolated rat liver mitochondria and hepatocytes. J Biochem. 1997 Jan;121(1):29-37.

    [2]. Jin T. et al. Near-infrared fluorescence detection of acetylcholine in aqueous solution using a complex ofrhodamine 800 and p-sulfonatocalix[8]arene. Sensors (Basel). 2010;10(3):2438-49.