荧光染料Dye Reagents 7-Amino-4-(trifluoromethyl)coumarin;(Synonyms: 香豆素151; Coumarin 151; AFC) 纯度: 99.10%
7-Amino-4-(trifluoromethyl)coumarin (Coumarin 151) 是荧光标记物,可用来灵敏的检测蛋白酶。它的激发和发射波长分别为400和500 nm。
7-Amino-4-(trifluoromethyl)coumarin Chemical Structure
CAS No. : 53518-15-3
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7-Amino-4-(trifluoromethyl)coumarin 相关产品
bull;相关化合物库:
- Bioactive Compound Library Plus
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- Medicine Food Homology Compound Library
生物活性 |
7-Amino-4-(trifluoromethyl)coumarin (Coumarin 151) is a fluorescent marker for the sensitive detection of proteinases. The excitation and emission wavelengths are 400 and 490 nm, respectively. |
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体外研究 (In Vitro) |
Amino acid and peptide derivatives of 7-amino-4-(trifluoromethyl)coumarin are used to monitor peptidase activities. Caspase activation is measured using the fluorogenic compound N-acetyl-asp-glu-val-asp-7-Amino-4-(trifluoromethyl)coumarin (Ac-DEVD-AFC). This substrate rapidly enters cells where it is efficiently cleaved at the aspartate residue by specific caspases, yielding the fluorescent compound 7-Amino-4-(trifluoromethyl)coumarin (AFC). Following cell disruption, released 7-Amino-4-(trifluoromethyl)coumarin is separated on HPLC and detected by fluorescence. The appearance of 7-Amino-4-(trifluoromethyl)coumarin in cells is blocked by the pancaspase inhibitor benzyloxycarbonyl-val-ala-asp-fluoromethylketone, thus establishing that intracellular caspases are responsible for the cleavage[1]. γ-glutamyl-7-amino-4-(trifluoro-methyl)coumarin is synthesized and used as a substrate for fluirimetric assay of γ-gluta-myltranspeptidase. The reaction product 7-amino-4-(trifluoromethyl)coumarin is fluorescent at neutral pH values and with excitation and emission wavelengths of 400 and 490 nm, respectively, concentration is linearly related to fluorescence over the range of 10 to 300 pmol/3 mL reaction mixture[2]. MCE has not independently confirmed the accuracy of these methods. They are for reference only. |
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分子量 |
229.16 |
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Formula |
C10H6F3NO2 |
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CAS 号 |
53518-15-3 |
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中文名称 |
香豆素151;7-氨基-4-氟甲醇 |
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运输条件 |
Room temperature in continental US; may vary elsewhere. |
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储存方式 |
4deg;C, protect from light *In solvent : -80deg;C, 6 months; -20deg;C, 1 month (protect from light) |
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溶解性数据 |
In Vitro:;
DMSO : 100 mg/mL (436.38 mM; Need ultrasonic) 配制储备液
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请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液;一旦配成溶液,请分装保存,避免反复冻融造成的产品失效。 In Vivo:
请根据您的实验动物和给药方式选择适当的溶解方案。以下溶解方案都请先按照 In Vitro 方式配制澄清的储备液,再依次添加助溶剂: ——为保证实验结果的可靠性,澄清的储备液可以根据储存条件,适当保存;体内实验的工作液,建议您现用现配,当天使用; 以下溶剂前显示的百
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参考文献 |
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